DIAGNÓSTICO DE BRUCELOSE EM BOVINOS NO ABATEDOURO MUNICIPAL DE IMPERATRIZ-MA

ABSTRACT: Through analysis of records of animals slaughtered and disposed of the municipal abattoir Imperatriz-MA we studied data from 39,955 animals slaughtered for consumption, and it was found that0.03% of the animals were discarded and the carcass incinerated due to contamination by Brucella abortus etiologic agent of brucellosis, one of the most serious zoonotic disease found in the country. The result was satisfactory thus demonstrating the effective control program against brucellosis carried out in the region.   KEY-WORDS: analysis, control, disposed, zoonosis.  RESUMO: Através de análise dos registros de animais abatidos no abatedouro municipal de Imperatriz-Ma, estudou-se os dados de 39.955 animais, e constatou-se que 0,03% dos animais tiveram a carcaça descartada e incinerada devido à contaminação por Brucela abortus, agente etiológico da brucelose, uma zoonose das mais graves constatadas no país. O resultado obtido foi satisfatório demonstrando assim o eficaz programa de controle contra brucelose realizado na região. PALAVRAS CHAVE: análise, controle, descartados, zoonose

FATORES DE RISCO PARA O CÂNCER DE PRÓSTATA: POPULAÇÃO DE UMA UNIDADE BÁSICA DE SAÚDE

The present investigation aimed to identify prostate cancer risk factors in men assisted in a basic health unit in the state of São Paulo in November 2015. It was a descriptive, cross-sectional and quantitative study, with a sample of 150 male users that went to the unit for a medical appointment and/or nursing care. The most relevant risk factors were age, level of education, sedentary lifestyle, consumption of alcohol, red meat, milk and fat and a high body mass index. The most used screening test was the prostate-specific antigen exam, followed by a rectal examination. The number of participants that presented at least one prostate cancer risk factor was high and that family history prompted men to seek prevention measures, such as appointments with urologists and screening exams.El estudio apuntó a identificar los factores de riesgo de cáncer de próstata entre hombres atendidos durante noviembre de 2015 en un Centro de Salud del Estado de São Paulo. Estudio descriptivo, transversal, de abordaje cuantitativo. Muestra integrada por 150 usuarios masculinos, que comparecieron en la unidad para realización de consulta y/o acogimiento de enfermería. Los factores de riesgo de mayor relevancia resultaron: edad, nivel de escolarización, sedentarismo, abuso de bebidas alcohólicas, ingesta de carnes rojas, leche y grasas, e Índice de Masa Corporal elevado. El examen de rastreo más realizado fue el de Antígeno Prostático Específico, seguido del tacto rectal. El número de participantes que presentó factores de riesgo de cáncer de próstata fue elevado. Se observó que la historia familiar de la enfermedad motiva a los hombres a buscar medidas preventivas, realizando consultas con el urólogo y análisis de rastreo.O estudo objetivou identificar os fatores de risco para o câncer de próstata entre homens atendidos durante o mês de novembro de 2015, em uma Unidade Básica de Saúde do estado de São Paulo. Estudo descritivo, transversal e de abordagem quantitativa, composta por 150 usuários homens que compareceram na unidade para realização de consulta e/ou acolhimento de enfermagem. Os fatores de risco com maior relevância foram idade, nível de escolaridade, sedentarismo, uso de bebida alcoólica, ingestão de carne vermelha, leite e gorduras e Índice de Massa Corporal aumentado. O exame de rastreio mais realizado foi o Antígeno Prostático Específico, seguido do toque retal. O número de participantes que apresentou algum fator de risco para o câncer de próstata foi elevado, observou-se que a história familiar da doença motiva os homens a procurarem por medidas de prevenção, com a realização de consulta com o urologista e exames de rastreio

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its 'Minimal Information for Studies of Extracellular Vesicles', which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly

COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study

Background: The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms. Methods: International, prospective observational study of 60 109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms. Results: ‘Typical’ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (≤ 18 years: 69, 48, 23; 85%), older adults (≥ 70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each P < 0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country. Interpretation: This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men

CHARACTERISTICS OF CARCASS OF INTACT CATTLE NELLORE AND F1 NELLORE X HOLSTEIN CARACTERÍSTICAS DE CARCAÇA DE NOVILHOS INTEIROS NELORE E F1 NELORE X HOLANDÊS

<p class="MsoNormal">Results of 14 intact cattle, with first dentition, were analyzed distinguishing the effects of Nellore and F1 Nellore x Holstein genetic groups in completely randomized blocks. The animals had the feeding system in a pasture of Brachiaria decumbens with 300 and 369.42 kg of initial and final weigh, respectively. The results shown greater thigh length to Nellore group (P<0.05). However the groups were similars (P>0.05) to live weight, cold and hot carcass weight, cold and hot dressing rendiment, break in cold, carcass length, arm perimeter, cushion thickness, fat thickness (FT), longissumus muscle area. Likely, the values to hindquarter, side and forequarter cuts in relation to cold and hot weight were equals (P>0.05). The concerns of the work are that the Nellore cattle are taller than F1 Nellore x Holstein and that this last group can be used to meat production, once the appraised are appropriated. </p> Keys-words: Carcass, crossing, cuts, cushion, yield. <p class="MsoNormal">Dados de quatorze novilhos com dentição de leite foram utilizados e distribuídos em dois grupos genéticos (sete bovinos da raça Nelore, sete bovinos F<sub>1</sub> Nelore x Holandês). Manejaram-se os animais em pastagem diferida de Brachiaria decumbens, iniciando-se o período experimental com 300 kg, sendo abatidos com 369,42 kg. O delineamento experimental foi blocos ao acaso. Os bovinos Nelore possuem maior (P<0,05) comprimento de perna que os animais F1 Nelore x Holandês. Entretanto não houve diferença (P>0,05) entre os grupos genéticos para peso vivo, peso da carcaça quente e fria, rendimento da carcaça quente e fria, quebra de resfriamento, comprimento da carcaça, perímetro do braço, espessura do coxão, espessura de gordura de cobertura (EGC), área de olho do lombo (AOL). Da mesma forma, houve semelhança (P>0,05) para o rendimento do corte serrote, costilhar e dianteiro em re-lação ao peso da carcaça quente e fria. Os bovinos Nelore são mais altos que os F1 Nelore x Holandês, e os machos F1 Nelore x Holandês podem ser utilizados para produção de carne, uma vez que as características da carcaça estudadas são consideradas satisfatórias.</p> Palavras-chaves: Cortes, coxão, carcaça, cruzamento, rendimento

Mapping the human genetic architecture of COVID-19

The genetic make-up of an individual contributes to the susceptibility and response to viral infection. Although environmental, clinical and social factors have a role in the chance of exposure to SARS-CoV-2 and the severity of COVID-191,2, host genetics may also be important. Identifying host-specific genetic factors may reveal biological mechanisms of therapeutic relevance and clarify causal relationships of modifiable environmental risk factors for SARS-CoV-2 infection and outcomes. We formed a global network of researchers to investigate the role of human genetics in SARS-CoV-2 infection and COVID-19 severity. Here we describe the results of three genome-wide association meta-analyses that consist of up to 49,562 patients with COVID-19 from 46 studies across 19 countries. We report 13 genome-wide significant loci that are associated with SARS-CoV-2 infection or severe manifestations of COVID-19. Several of these loci correspond to previously documented associations to lung or autoimmune and inflammatory diseases3–7. They also represent potentially actionable mechanisms in response to infection. Mendelian randomization analyses support a causal role for smoking and body-mass index for severe COVID-19 although not for type II diabetes. The identification of novel host genetic factors associated with COVID-19 was made possible by the community of human genetics researchers coming together to prioritize the sharing of data, results, resources and analytical frameworks. This working model of international collaboration underscores what is possible for future genetic discoveries in emerging pandemics, or indeed for any complex human disease

COVID-19 symptoms at hospital admission vary with age and sex: results from the ISARIC prospective multinational observational study

Background: The ISARIC prospective multinational observational study is the largest cohort of hospitalized patients with COVID-19. We present relationships of age, sex, and nationality to presenting symptoms. Methods: International, prospective observational study of 60 109 hospitalized symptomatic patients with laboratory-confirmed COVID-19 recruited from 43 countries between 30 January and 3 August 2020. Logistic regression was performed to evaluate relationships of age and sex to published COVID-19 case definitions and the most commonly reported symptoms. Results: ‘Typical’ symptoms of fever (69%), cough (68%) and shortness of breath (66%) were the most commonly reported. 92% of patients experienced at least one of these. Prevalence of typical symptoms was greatest in 30- to 60-year-olds (respectively 80, 79, 69%; at least one 95%). They were reported less frequently in children (≤ 18 years: 69, 48, 23; 85%), older adults (≥ 70 years: 61, 62, 65; 90%), and women (66, 66, 64; 90%; vs. men 71, 70, 67; 93%, each P < 0.001). The most common atypical presentations under 60 years of age were nausea and vomiting and abdominal pain, and over 60 years was confusion. Regression models showed significant differences in symptoms with sex, age and country. Interpretation: This international collaboration has allowed us to report reliable symptom data from the largest cohort of patients admitted to hospital with COVID-19. Adults over 60 and children admitted to hospital with COVID-19 are less likely to present with typical symptoms. Nausea and vomiting are common atypical presentations under 30 years. Confusion is a frequent atypical presentation of COVID-19 in adults over 60 years. Women are less likely to experience typical symptoms than men

Association of Country Income Level With the Characteristics and Outcomes of Critically Ill Patients Hospitalized With Acute Kidney Injury and COVID-19

Introduction: Acute kidney injury (AKI) has been identified as one of the most common and significant problems in hospitalized patients with COVID-19. However, studies examining the relationship between COVID-19 and AKI in low- and low-middle income countries (LLMIC) are lacking. Given that AKI is known to carry a higher mortality rate in these countries, it is important to understand differences in this population. Methods: This prospective, observational study examines the AKI incidence and characteristics of 32,210 patients with COVID-19 from 49 countries across all income levels who were admitted to an intensive care unit during their hospital stay. Results: Among patients with COVID-19 admitted to the intensive care unit, AKI incidence was highest in patients in LLMIC, followed by patients in upper-middle income countries (UMIC) and high-income countries (HIC) (53%, 38%, and 30%, respectively), whereas dialysis rates were lowest among patients with AKI from LLMIC and highest among those from HIC (27% vs. 45%). Patients with AKI in LLMIC had the largest proportion of community-acquired AKI (CA-AKI) and highest rate of in-hospital death (79% vs. 54% in HIC and 66% in UMIC). The association between AKI, being from LLMIC and in-hospital death persisted even after adjusting for disease severity. Conclusions: AKI is a particularly devastating complication of COVID-19 among patients from poorer nations where the gaps in accessibility and quality of healthcare delivery have a major impact on patient outcomes

Thrombotic and hemorrhagic complications of COVID-19 in adults hospitalized in high-income countries compared with those in adults hospitalized in low- and middle-income countries in an international registry

Background: COVID-19 has been associated with a broad range of thromboembolic, ischemic, and hemorrhagic complications (coagulopathy complications). Most studies have focused on patients with severe disease from high-income countries (HICs). Objectives: The main aims were to compare the frequency of coagulopathy complications in developing countries (low- and middle-income countries [LMICs]) with those in HICs, delineate the frequency across a range of treatment levels, and determine associations with in-hospital mortality. Methods: Adult patients enrolled in an observational, multinational registry, the International Severe Acute Respiratory and Emerging Infections COVID-19 study, between January 1, 2020, and September 15, 2021, met inclusion criteria, including admission to a hospital for laboratory-confirmed, acute COVID-19 and data on complications and survival. The advanced-treatment cohort received care, such as admission to the intensive care unit, mechanical ventilation, or inotropes or vasopressors; the basic-treatment cohort did not receive any of these interventions. Results: The study population included 495,682 patients from 52 countries, with 63% from LMICs and 85% in the basic treatment cohort. The frequency of coagulopathy complications was higher in HICs (0.76%-3.4%) than in LMICs (0.09%-1.22%). Complications were more frequent in the advanced-treatment cohort than in the basic-treatment cohort. Coagulopathy complications were associated with increased in-hospital mortality (odds ratio, 1.58; 95% CI, 1.52-1.64). The increased mortality associated with these complications was higher in LMICs (58.5%) than in HICs (35.4%). After controlling for coagulopathy complications, treatment intensity, and multiple other factors, the mortality was higher among patients in LMICs than among patients in HICs (odds ratio, 1.45; 95% CI, 1.39-1.51). Conclusion: In a large, international registry of patients hospitalized for COVID-19, coagulopathy complications were more frequent in HICs than in LMICs (developing countries). Increased mortality associated with coagulopathy complications was of a greater magnitude among patients in LMICs. Additional research is needed regarding timely diagnosis of and intervention for coagulation derangements associated with COVID-19, particularly for limited-resource settings

Thrombotic and hemorrhagic complications of COVID-19 in adults hospitalized in high-income countries compared with those in adults hospitalized in low- and middle-income countries in an international registry

Background: COVID-19 has been associated with a broad range of thromboembolic, ischemic, and hemorrhagic complications (coagulopathy complications). Most studies have focused on patients with severe disease from high-income countries (HICs). Objectives: The main aims were to compare the frequency of coagulopathy complications in developing countries (low- and middle-income countries [LMICs]) with those in HICs, delineate the frequency across a range of treatment levels, and determine associations with in-hospital mortality. Methods: Adult patients enrolled in an observational, multinational registry, the International Severe Acute Respiratory and Emerging Infections COVID-19 study, between January 1, 2020, and September 15, 2021, met inclusion criteria, including admission to a hospital for laboratory-confirmed, acute COVID-19 and data on complications and survival. The advanced-treatment cohort received care, such as admission to the intensive care unit, mechanical ventilation, or inotropes or vasopressors; the basic-treatment cohort did not receive any of these interventions. Results: The study population included 495,682 patients from 52 countries, with 63% from LMICs and 85% in the basic treatment cohort. The frequency of coagulopathy complications was higher in HICs (0.76%-3.4%) than in LMICs (0.09%-1.22%). Complications were more frequent in the advanced-treatment cohort than in the basic-treatment cohort. Coagulopathy complications were associated with increased in-hospital mortality (odds ratio, 1.58; 95% CI, 1.52-1.64). The increased mortality associated with these complications was higher in LMICs (58.5%) than in HICs (35.4%). After controlling for coagulopathy complications, treatment intensity, and multiple other factors, the mortality was higher among patients in LMICs than among patients in HICs (odds ratio, 1.45; 95% CI, 1.39-1.51). Conclusion: In a large, international registry of patients hospitalized for COVID-19, coagulopathy complications were more frequent in HICs than in LMICs (developing countries). Increased mortality associated with coagulopathy complications was of a greater magnitude among patients in LMICs. Additional research is needed regarding timely diagnosis of and intervention for coagulation derangements associated with COVID-19, particularly for limited-resource settings