Colonial Organization of Mine Labour in Charcas 1 (Present-Day Bolivia) and Its Consequences 2 (Sixteenth to the Seventeenth Centuries)

This article analyses the changes in the organization of labour during the sixteenth and seventeenth centuries in colonial Charcas, present-day Bolivia, focusing on the role that different colonial authorities played in this process and its consequences. The Spanish took advantage of the pre-Hispanic organization of labour from the beginning of their conquest. However, in a colonial context, labour relations changed significantly, and the architect of those alterations was Viceroy Francisco de Toledo. We examine the transformations in mine labour carried out by the Spanish colonial polity; these had a significant effect not only on mining, but also on all labour relations in the southern colonial Andes.Fil: Gil Montero, Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Instituto de Geografía, Historia y Ciencias Sociales. Universidad Nacional del Centro de la Provincia de Buenos Aires. Instituto de Geografía, Historia y Ciencias Sociales; ArgentinaFil: Zagalsky, Paula Cecilia. Universidad Nacional de Jujuy; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Hygiene and The Indian Problem : Ethnicity and Medicine in Bolivia, 1910-1920

In the first decades of the twentieth century, Bolivian intellectuals and politicians debated how the country\u27s Indian population should be incorporated into social and political life as the nation became increasingly integrated internally and forged stronger links to the world market. Public health was central to this discussion because of elite fears of contagion due to greater contact between Indians and non-Indians and the realization that if Indians were to be productive members of society, then their physical well-being had to be considered. This study examines the proposals of two Bolivian doctors, Jaime Mendoza and Nestor Morales, for improving the health of the native population in the context of the larger national debate about ethnicity and citizenship

PhosPhAt goes kinases--searchable protein kinase target information in the plant phosphorylation site database PhosPhAt

Reversible phosphorylation is a key mechanism for regulating protein function. Thus it is of high interest to know which kinase can phosphorylate which proteins. Comprehensive information about phosphorylation sites in Arabidopsis proteins is hosted within the PhosPhAt database (http://phosphat.mpimp-golm.mpg.de). However, our knowledge of the kinases that phosphorylate those sites is dispersed throughout the literature and very difficult to access, particularly for investigators seeking to interpret large scale and high-throughput experiments. Therefore, we aimed to compile information on kinase-substrate interactions and kinase-specific regulatory information and make this available via a new functionality embedded in PhosPhAt. Our approach involved systematic surveying of the literature for regulatory information on the members of the major kinase families in Arabidopsis thaliana, such as CDPKs, MPK(KK)s, AGC kinases and SnRKs, as well as individual kinases from other families. To date, we have researched more than 4450 kinase-related publications, which collectively contain information on about 289 kinases. Users can now query the PhosPhAt database not only for experimental and predicted phosphorylation sites of individual proteins, but also for known substrates for a given kinase or kinase family. Further developments include addition of new phosphorylation sites and visualization of clustered phosphorylation events, known as phosphorylation hotspots

Environment, Urbanization, and Public Health: The Bubonic Plague Epidemic of 1912 in San Juan, Puerto Rico

In this article I focus on the nexus of urbanization, the environment and public health that was exposed by the 1912 bubonic plague epidemic in San Juan, Puerto Rico. I utilize the concept of “urban health penalty,” developed by demographic historians of the industrial revolution in Europe, to account for the declining health of the working class in cities. While in Europe poor health among the urban poor was associated with industrialization, I argue that in San Juan chaotic, unplanned urbanization reflected the effects of colonialism. I further examine how the epidemic exacerbated the class and racial divisions that contributed to the disastrous living conditions, and speculate as to whether the environmental neglect of the neighborhood by authorities was due to the fact that it was home to many poor people of African descent. I also highlight the varying understandings of the causes and appropriate responses to plague.  En este artículo me centro en el nexo de la urbanización, el medio ambiente y la salud pública que fue expuesto por la epidemia de peste bubónica de 1912 en San Juan, Puerto Rico. Utilizo el concepto de “urban health penalty” desarrollado por los historiadores demográficos de la revolución industrial en Europa, para explicar la disminución de la salud de la clase trabajadora en las ciudades. Mientras que en Europa la mala salud de los pobres urbanos estaba asociada con la industrialización, sostengo que en San Juan la urbanización caótica y no planeada reflejaba los efectos del colonialismo. Examino además cómo la epidemia exacerbó las divisiones de clase y raza que contribuyeron a las desastrosas condiciones de vida y pregunto si la negligencia ambiental del vecindario por parte de las autoridades se debía al hecho de que era el hogar de muchos pobres de ascendencia africana. También resalto los diversos entendimientos de las causas y las respuestas apropiadas a la peste

Aufgaben und Herausforderungen einer vielfältigen und vielfaltsfördernden Pflanzenzüchtung. Endbericht zum TA-Projekt »Herausforderungen für die Pflanzenzüchtung – Auswirkungen des Strukturwandels in der Pflanzenzüchtung auf die genetische Diversität, die Sortenvielfalt und die Leistungsfähigkeit der heimischen Landwirtschaft«

Die Pflanzenzüchtung ist ein zentrales Element einer zukunftsfähigen, nachhaltigen Landwirtschaft. Deren Ausgestaltung ist eine der großen politischen und gesellschaftlichen Zukunftsaufgaben in einem komplexen Spannungsfeld verschiedener Kontroversen über ökologische, ökonomische, soziale und (tier)ethische Herausforderungen. Ein Gesamtkonzept für ein zukunftsfähiges, nachhaltiges Landwirtschafts- und Ernährungssystem wäre eine wichtige Voraussetzung dafür, dass die Pflanzenzüchtung als der Landwirtschaft vorgelagerter Sektor zur Lösung dieser Zukunftsaufgaben bestmöglich beitragen kann. Sowohl aus Gründen der Zukunftsvorsorge als auch in forschungs- und innovationspolitischer Hinsicht gilt die Förderung der Pflanzenzüchtung bzw. der Züchtungsforschung als wichtige öffentliche Aufgabe. Die vorliegende Untersuchung, die vom Ausschuss für Bildung, Forschung und Technikfolgenabschätzung auf Anregung des Ausschusses für Ernährung und Landwirtschaft in Auftrag gegeben wurde, gibt einen Überblick über Potenziale und Aufgaben, Stärken und Schwächen der deutschen (konventionellen und ökologischen) Pflanzenzüchtung im Kontext einer ressourcenschonenden, nachhaltigen Landwirtschaft angesichts von Klimawandel, Biodiversitätsverlust, Bedürfnissen einer weiter wachsenden Weltbevölkerung sowie dem Biomassebedarf einer zukünftigen Bioökonomie

Autophosphorylation-based calcium (Ca2+) sensitivity priming and Ca2+/Calmodulin inhibition of Arabidopsis thaliana Ca2+-dependent protein kinase 28 (CPK28)

Plant calcium (Ca2+) dependent protein kinases (CPKs) are composed of a dual specificity (Ser/Thr and Tyr) kinase domain tethered to a Calmodulin-like domain (CLD) via an autoinhibitory junction (J) and represent the primary Ca2+-dependent protein kinase activities in plant systems. While regulation of CPKs by Ca2+ has been extensively studied, the contribution of autophosphorylation in the control of CPK activity is less well understood. Furthermore, whether Calmodulin (CaM) contributes to CPK regulation, as is the case for Ca2+/CaM-dependent protein kinases (CaMKs) outside the plant lineage, remains an open question. We screened a subset of plant CPKs for CaM-binding and found that CPK28 is a high-affinity Ca2+/CaM-binding protein. Using synthetic peptides and native gel electrophoresis, we coarsely mapped the CaM-binding domain to a site within the CPK28 J domain that overlaps with the known site of intramolecular interaction between the J domain and CLD. Peptide kinase activity of fully dephosphorylated CPK28 was Ca2+-responsive and inhibited by Ca2+/CaM. Using in situ autophosphorylated protein, we expand on the known set of CPK28 autophosphorylation sites, and demonstrate that, unexpectedly, autophosphorylated CPK28 had enhanced activity at physiological concentrations of Ca2+ compared to dephosphorylated protein, suggesting that autophosphorylation functions to prime CPK28 for Ca2+-activation. Furthermore, CPK28 autophosphorylation substantially reduced sensitivity of the kinase to Ca2+/CaM inhibition. Overall, our analyses uncover new complexities in the control of CPK28 and provide mechanistic support for Ca2+ signaling specificity through Ca2+ sensor priming

Police interrogation practice in Slovenia

Interrogation techniques are well explored, but in Slovenia it has remained unknown what interrogation techniques are used and what the basic characteristics of suspect interrogations are. The Slovenian interrogation manual proposes some coercive interrogation techniques and neglects their weaknesses. The aim of the current study was to examine Slovenian police officers’ beliefs as to the basic characteristics of their interrogations and whether techniques proposed by the manual are used in practice to begin to provide some insight into what actually happens in such interrogations. A survey instrument was used to obtain selfreport data from a sample of criminal investigators. From 86 completed questionnaires it was found that a typical interrogation of a suspect lasts around 90 minutes and is not recorded. Interviewers typically use three interrogation techniques namely (i) conducting interrogations in isolation; (ii) identifying contradictions in the suspect’s story; and (iii) confronting the suspect with evidence. Findings suggest that some coercive interrogation techniques are used in practice (e.g. offering moral justifications, alluding to have evidence of guilt, good cop/bad cop routine, and minimization). The study is the first insight into the practices of Slovenian investigators when questioning suspects. Differences among general, white-collar and organized crime investigators are also discussed

SUPERSHARP - Segmented Unfolding Primary for Exoplanet Research via Spectroscopic High Angular Resolution Photography

We propose to search for biosignatures in the spectra of reflected light from about 100 Earth-sized planets that are already known to be orbiting in their habitable zones (HZ). For a sample of G and K type hosts, most of these planets will be between 25 and 50 milli-arcsec (mas) from their host star and 1 billion to 10 billion times fainter. To separate the planet's image from that of its host star at the wavelength (763nm) of the oxygen biosignature we need a telescope with an aperture of 16 metres. Furthermore, the intensity of the light from the host star at the position in the image of the exoplanet must be suppressed otherwise the exoplanet will be lost in the glare. This presents huge technical challenges. The Earth's atmosphere is turbulent which makes it impossible to achieve the required contrast from the ground at 763nm. The telescope therefore needs to be in space and to fit the telescope in the rocket fairing it must be a factor of 4 or more times smaller when folded than when operational. To obtain spectroscopy of the planet's biosignature at 763nm we need to use an integral field spectrometer (IFS) with a field of view (FOV) of 1000 x 1000 milli-arcsec (mas) and a spectral resolution of 100. This is a device that simultaneously takes many pictures of the exoplanet each at a slightly different wavelength which are then recorded as a data cube with two spatial dimensions and one wavelength dimension. In every data cube wavelength slice, the background light from the host star at the location of the planet image must be minimised. This is achieved via a coronagraph which blocks the light from the host star and active/adaptive optics techniques which continuously maintain very high accuracy optical alignment to make the images as sharp as possible. These are the technical challenges to be addressed in a design study.Comment: A proposal in response to the ESA New Science Ideas call. Sept 2016. 25 page

SUPERSHARP - Segmented Unfolding Primary for Exoplanet Research via Spectroscopic High Angular Resolution Photography

A proposal in response to the ESA New Science Ideas call. Sept 2016. 25 pagesWe propose to search for biosignatures in the spectra of reflected light from about 100 Earth-sized planets that are already known to be orbiting in their habitable zones (HZ). For a sample of G and K type hosts, most of these planets will be between 25 and 50 milli-arcsec (mas) from their host star and 1 billion to 10 billion times fainter. To separate the planet's image from that of its host star at the wavelength (763nm) of the oxygen biosignature we need a telescope with an aperture of 16 metres. Furthermore, the intensity of the light from the host star at the position in the image of the exoplanet must be suppressed otherwise the exoplanet will be lost in the glare. This presents huge technical challenges. The Earth's atmosphere is turbulent which makes it impossible to achieve the required contrast from the ground at 763nm. The telescope therefore needs to be in space and to fit the telescope in the rocket fairing it must be a factor of 4 or more times smaller when folded than when operational. To obtain spectroscopy of the planet's biosignature at 763nm we need to use an integral field spectrometer (IFS) with a field of view (FOV) of 1000 x 1000 milli-arcsec (mas) and a spectral resolution of 100. This is a device that simultaneously takes many pictures of the exoplanet each at a slightly different wavelength which are then recorded as a data cube with two spatial dimensions and one wavelength dimension. In every data cube wavelength slice, the background light from the host star at the location of the planet image must be minimised. This is achieved via a coronagraph which blocks the light from the host star and active/adaptive optics techniques which continuously maintain very high accuracy optical alignment to make the images as sharp as possible. These are the technical challenges to be addressed in a design study

Characterization of auxin transporter PIN6 plasma membrane targeting reveals a function for PIN6 in plant bolting

Auxin gradients are sustained by series of influx and efflux carriers whose subcellular localization is sensitive to both exogenous and endogenous factors. Recently the localization of the Arabidopsis thaliana auxin efflux carrier PIN-FORMED (PIN) 6 was reported to be tissue-specific and regulated through unknown mechanisms. Here, we used genetic, molecular and pharmacological approaches to characterize the molecular mechanism(s) controlling the subcellular localization of PIN6. PIN6 localizes to endomembrane domains in tissues with low PIN6 expression levels such as roots, but localizes at the plasma membrane (PM) in tissues with increased PIN6 expression such as the inflorescence stem and nectary glands. We provide evidence that this dual localization is controlled by PIN6 phosphorylation and demonstrate that PIN6 is phosphorylated by mitogen-activated protein kinases (MAPKs) MPK4 and MPK6. The analysis of transgenic plants expressing PIN6 at PM or in endomembrane domains reveals that PIN6 subcellular localization is critical for Arabidopsis inflorescence stem elongation post-flowering (bolting). In line with a role for PIN6 in plant bolting, inflorescence stems elongate faster in pin6 mutant plants than in wild-type plants. We propose that PIN6 subcellular localization is under the control of developmental signals acting on tissue-specific determinants controlling PIN6-expression levels and PIN6 phosphorylation