This article analyses the changes in the organization of labour during the sixteenth and seventeenth centuries in colonial Charcas, present-day Bolivia, focusing on the role that different colonial authorities played in this process and its consequences. The Spanish took advantage of the pre-Hispanic organization of labour from the beginning of their conquest. However, in a colonial context, labour relations changed significantly, and the architect of those alterations was Viceroy Francisco de Toledo. We examine the transformations in mine labour carried out by the Spanish colonial polity; these had a significant effect not only on mining, but also on all labour relations in the southern colonial Andes.Fil: Gil Montero, Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Instituto de Geografía, Historia y Ciencias Sociales. Universidad Nacional del Centro de la Provincia de Buenos Aires. Instituto de Geografía, Historia y Ciencias Sociales; ArgentinaFil: Zagalsky, Paula Cecilia. Universidad Nacional de Jujuy; Argentina. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin