La flore médicinale traditionnelle de la région de Béni- Mellal (Maroc)

Objectif : le but de cette étude est de mener une enquête ethnobotanique des plantes médicinales utilisées en phytothérapie traditionnelle dans le district de Béni-Mellal.Méthodologie et résultats : A l’aide de 200 fiches questionnaires, une série d’enquêtes  ethnobotaniques a été menée sur le terrain en 2014 en utilisant un échantillonnage aléatoire stratifié. Ces recherches, complétées par l’identification des échantillons sur le terrain et au laboratoire, nous ont permis de faire connaissance de 69 espèces médicinales réparties en 38 familles et 63 genres avec une importance relative des familles suivantes par leur nombre d’espèces : Lamiaceae (23,07%), Apiaceae (21,05%), Fabaceae (10,52%) et Asteraceae (7,69%).Conclusion et application de la recherche : Cette étude a conduit à l'élaboration d'un catalogue de plantes médicinales contenant les informations sur les utilisations thérapeutiques pratiquées par la population locale. Par ailleurs, Cet inventaire peut constituer une source d’informations très précieuse .Il contribue par sa part à la connaissance de la flore médicinale et la sauvegarde du savoir-faire populaire local.Mots clés : plantes médicinales, ethnobotanique, Béni-Mellal, flore médicinale.Objective: The aim of this study is to conduct an ethnobotanical survey of medicinal plants used in traditional herbal medicine in Beni-Mellal district.Methods and results: Using 320 questionnaires, a series of ethnobotanical surveys was conducted in the field in 2014 using stratified random sampling techniques. This research, completed by the identification of samples in the field and in the laboratory, has allowed us to get to know 69 species belonging to 63 genera and 38 families, with a predominance of Lamiaceae (23.07%), Apiaceae (21.05%), Fabaceae (10,52%) and Asteraceae (7.69% %).Conclusion and application of research: This study has led to the development of a catalog of  medicinal plants which has the information about the therapeutic uses practiced by the local population. This inventory can be a very valuable source of information .It contributes its share to the knowledge of medicinal flora and the safeguarding of local popular expertise.Keywords: Medicinal plants, ethnobotany, Beni-Mellal, medicinal flora

Study of Bryophytic Flora in the Ramsar Wetland of Merja Zerga (North-west of Morocco)

The research undertaken in the IBA and Ramsar wetland of Merja Zerga at Moulay Bousselham were interested only in the higher plants. Our study tends then to contribute to enrich the knowledge about the bryophytic flora of this area by filling the gaps in this field. Thus, we carried out a systematic sampling with a stop and a harvest in each encountered bryophyte population. This prospection enabled us to find 26 species of bryophytes including 22 species of mosses belonging to 15 genera and 9 families, and 4 species of liverworts belonging to 3 genera and 3 families. The relative low specificity of this wetland can be explained by the influence of sea spray and the strong anthropic disturbance. A comparison with previous studies allowed us to conclude that 11 species were observed for the first time in the area

Deux cas béninois d’entomophthoromycoses (basidiobolomycose et conidiobolomycose) diagnostiqués en 2010 au CHU d’Angers

National audienc

Conidiobolomycose rhinofaciale avec localisations cervicales, thoraciques et brachiales : à propos d’un cas nigérian

We report here the clinical case of a Nigerian adult patient who received medical care during October 2010, at the Center for Diagnosis and Treatment of Buruli ulcer in Pobè (Benin). He presented a massive facial tumor associated with several subcutaneous (cervical, thoracic and upper limbs) nodules, evolving since several years. Tissue samples collected at Pobè medical center were addressed to the mycology and histology laboratories of Angers University Hospital (France), according to the medical exchange agreement between the two institutions about the diagnosis and treatment of Buruli ulcer disease. Histological examination showed a Splendore-Hoeppli phenomenon, consisting of a granulomatous reaction made of eosinophilic polynuclear cells surrounding rare, large and irregular, non-septate hyphae. A filamentous fungus was isolated by cultivation of the clinical samples, which was identified as Conidiobolus coronatus. The patient was treated orally with daily doses of ketoconazole (400 mg per day). After 4 months of treatment, a marked regression of the facial lesion was obtained. A first constructive facial surgery was achieved, but the patient did not attend the second step. This case report allows us to remind the mycological diagnosis of this exotic mycosis, but also to emphasize the main difficulties encountered in medical management in the developing countries

Study of the bryological flora at the archaeological site of Chellah, Morocco

The Chellah archaeological site in Rabat, listed as a cultural asset since 2012 on UNESCO's World Heritage List, is subject to significant biodeterioration. The aim of this study is to identify the bryophytes that have an important impact on the destruction of the substrate. For this purpose, three prospectionswere carried out in autumn 2014, spring 2015 and winter 2016. The systematic sampling carried out allowed us to identify 20 species of bryophytes belonging to 10 botanical families, of which 4are dominant with 13 species equivalent to 65% of the total. The four families are Pottiaceae, Brachyceciaceae, Funariaceae and Bryaceae; they belong to the class of Muscinae. The liverworts are represented by only 6 species, representing 30% of the total population. Among the 20 species inventoried, 3 are newly observed in the region of Rabat: Entosthodon pulchellus (H. Philib.) Brugués; Dydimodon Fallax Hedw. and Trichostomum crispulum Bruch. When bryophytes settle on substrates, a preliminary soilis initiated to the detriment of the quality of materials and their durability

Azapeptide activity-based probes for the SARS-CoV-2 main protease enable visualization of inhibition in infected cells

The COVID-19 pandemic has revealed the vulnerability of the modern, global society. With expected waves of future infections by SARS-CoV-2, treatment options for infected individuals will be crucial in order to decrease mortality and hospitalizations. The SARS-CoV-2 main protease is a validated drug target, for which the first inhibitor has been approved for use in patients. To facilitate future work on this drug target, we designed a solid-phase synthesis route towards azapeptide activity-based probes that are capped with a cysteine-reactive electrophile for covalent modification of the active site of Mpro. This design led to the most potent ABP for Mpro and one of the most potent inhibitors reported thus far. We demonstrate that this ABP can be used to visualize Mpro activity and target engagement by drugs in infected cells

Analysis of the Legionella longbeachae Genome and Transcriptome Uncovers Unique Strategies to Cause Legionnaires' Disease

Legionella pneumophila and L. longbeachae are two species of a large genus of bacteria that are ubiquitous in nature. L. pneumophila is mainly found in natural and artificial water circuits while L. longbeachae is mainly present in soil. Under the appropriate conditions both species are human pathogens, capable of causing a severe form of pneumonia termed Legionnaires' disease. Here we report the sequencing and analysis of four L. longbeachae genomes, one complete genome sequence of L. longbeachae strain NSW150 serogroup (Sg) 1, and three draft genome sequences another belonging to Sg1 and two to Sg2. The genome organization and gene content of the four L. longbeachae genomes are highly conserved, indicating strong pressure for niche adaptation. Analysis and comparison of L. longbeachae strain NSW150 with L. pneumophila revealed common but also unexpected features specific to this pathogen. The interaction with host cells shows distinct features from L. pneumophila, as L. longbeachae possesses a unique repertoire of putative Dot/Icm type IV secretion system substrates, eukaryotic-like and eukaryotic domain proteins, and encodes additional secretion systems. However, analysis of the ability of a dotA mutant of L. longbeachae NSW150 to replicate in the Acanthamoeba castellanii and in a mouse lung infection model showed that the Dot/Icm type IV secretion system is also essential for the virulence of L. longbeachae. In contrast to L. pneumophila, L. longbeachae does not encode flagella, thereby providing a possible explanation for differences in mouse susceptibility to infection between the two pathogens. Furthermore, transcriptome analysis revealed that L. longbeachae has a less pronounced biphasic life cycle as compared to L. pneumophila, and genome analysis and electron microscopy suggested that L. longbeachae is encapsulated. These species-specific differences may account for the different environmental niches and disease epidemiology of these two Legionella species

Preferential Paths of Air-water Two-phase Flow in Porous Structures with Special Consideration of Channel Thickness Effects.

Accurate understanding and predicting the flow paths of immiscible two-phase flow in rocky porous structures are of critical importance for the evaluation of oil or gas recovery and prediction of rock slides caused by gas-liquid flow. A 2D phase field model was established for compressible air-water two-phase flow in heterogenous porous structures. The dynamic characteristics of air-water two-phase interface and preferential paths in porous structures were simulated. The factors affecting the path selection of two-phase flow in porous structures were analyzed. Transparent physical models of complex porous structures were prepared using 3D printing technology. Tracer dye was used to visually observe the flow characteristics and path selection in air-water two-phase displacement experiments. The experimental observations agree with the numerical results used to validate the accuracy of phase field model. The effects of channel thickness on the air-water two-phase flow behavior and paths in porous structures were also analyzed. The results indicate that thick channels can induce secondary air flow paths due to the increase in flow resistance; consequently, the flow distribution is different from that in narrow channels. This study provides a new reference for quantitatively analyzing multi-phase flow and predicting the preferential paths of immiscible fluids in porous structures

Genome Sequence of the Saprophyte Leptospira biflexa Provides Insights into the Evolution of Leptospira and the Pathogenesis of Leptospirosis

Leptospira biflexa is a free-living saprophytic spirochete present in aquatic environments. We determined the genome sequence of L. biflexa, making it the first saprophytic Leptospira to be sequenced. The L. biflexa genome has 3,590 protein-coding genes distributed across three circular replicons: the major 3,604 chromosome, a smaller 278-kb replicon that also carries essential genes, and a third 74-kb replicon. Comparative sequence analysis provides evidence that L. biflexa is an excellent model for the study of Leptospira evolution; we conclude that 2052 genes (61%) represent a progenitor genome that existed before divergence of pathogenic and saprophytic Leptospira species. Comparisons of the L. biflexa genome with two pathogenic Leptospira species reveal several major findings. Nearly one-third of the L. biflexa genes are absent in pathogenic Leptospira. We suggest that once incorporated into the L. biflexa genome, laterally transferred DNA undergoes minimal rearrangement due to physical restrictions imposed by high gene density and limited presence of transposable elements. In contrast, the genomes of pathogenic Leptospira species undergo frequent rearrangements, often involving recombination between insertion sequences. Identification of genes common to the two pathogenic species, L. borgpetersenii and L. interrogans, but absent in L. biflexa, is consistent with a role for these genes in pathogenesis. Differences in environmental sensing capacities of L. biflexa, L. borgpetersenii, and L. interrogans suggest a model which postulates that loss of signal transduction functions in L. borgpetersenii has impaired its survival outside a mammalian host, whereas L. interrogans has retained environmental sensory functions that facilitate disease transmission through water

Mechanism of Heparin Acceleration of Tissue Inhibitor of Metalloproteases-1 (TIMP-1) Degradation by the Human Neutrophil Elastase

Heparin has been shown to regulate human neutrophil elastase (HNE) activity. We have assessed the regulatory effect of heparin on Tissue Inhibitor of Metalloproteases-1 [TIMP-1] hydrolysis by HNE employing the recombinant form of TIMP-1 and correlated FRET-peptides comprising the TIMP-1 cleavage site. Heparin accelerates 2.5-fold TIMP-1 hydrolysis by HNE. The kinetic parameters of this reaction were monitored with the aid of a FRET-peptide substrate that mimics the TIMP-1 cleavage site in pre-steady-state conditionsby using a stopped-flow fluorescence system. The hydrolysis of the FRET-peptide substrate by HNE exhibits a pre-steady-state burst phase followed by a linear, steady-state pseudo-first-order reaction. The HNE acylation step (k2 = 21±1 s−1) was much higher than the HNE deacylation step (k3 = 0.57±0.05 s−1). The presence of heparin induces a dramatic effect in the pre-steady-state behavior of HNE. Heparin induces transient lag phase kinetics in HNE cleavage of the FRET-peptide substrate. The pre-steady-state analysis revealed that heparin affects all steps of the reaction through enhancing the ES complex concentration, increasing k1 2.4-fold and reducing k−1 3.1-fold. Heparin also promotes a 7.8-fold decrease in the k2 value, whereas the k3 value in the presence of heparin was increased 58-fold. These results clearly show that heparin binding accelerates deacylation and slows down acylation. Heparin shifts the HNE pH activity profile to the right, allowing HNE to be active at alkaline pH. Molecular docking and kinetic analysis suggest that heparin induces conformational changes in HNE structure. Here, we are showing for the first time that heparin is able to accelerate the hydrolysis of TIMP-1 by HNE. The degradation of TIMP-1is associated to important physiopathological states involving excessive activation of MMPs