Proton reduction using cobalt glyoximes with isothiocyanate and aniline axial ligands

10.1016/j.poly.2015.04.030Polyhedron9638-4

Attraction of semiconductor nanowires: an in situ observation

In situ deformation transmission electron microscopy was used to study the attraction behavior of GaAs semiconductor nanowires (NWs). The NWs demonstrated an interesting phenomenon of either head-to-head or body-to-body attraction at distances that depend on the NW diameters. The NWs with a diameter of ∼25 nm attracted at a distance of ∼25 nm, while large-diameter NWs of ∼55 nm showed no obvious attraction. The underlying mechanism governing the attraction of the NWs is proposed and discussed with a mechanistic model. The diameter dependence on the NW attraction behavior is discussed. The finding provides an understanding of the Ampère force in nanostructured materials caused by an electron-beam-induced current while technologically it provides useful hints for designing NW-based devices according to the diameter-dependent attraction behavior of NWs

Conducting expeditionary operations in the contested littorals

Reissued 21 Oct 2015 to revise figure cross references in body text.The United States armed services have identified capability gaps in the areas of company-sized raid and sustainment operations in contested littoral environments. Multiple joint platform packages can be employed to provide the required mission capabilities to fill the gap. This thesis identifies the operational, functional, and physical architecture and effectiveness of mission packages necessary to provide capabilities associated with littoral sustainment operations. Physical architecture configurations are evaluated using discrete event modeling. Cost and performance estimates for the mission packages are presented in order to provide the decision maker tools for identifying which alternative provides the most cost-effective solution for the needs of a scenario’s stakeholders. This thesis report concludes by identifying potential assets that would provide cost-effective support of littoral operations. Feasible alternatives provide varying levels of effectiveness in terms of average deployment time and percentage of threats successfully affected.http://archive.org/details/conductingexpedi1094546910Approved for public release; distribution is unlimited

A comprehensive synthetic library of poly-N-acetyl glucosamines enabled vaccine against lethal challenges of Staphylococcus aureus

Abstract Poly-β-(1–6)-N-acetylglucosamine (PNAG) is an important vaccine target, expressed on many pathogens. A critical hurdle in developing PNAG based vaccine is that the impacts of the number and the position of free amine vs N-acetylation on its antigenicity are not well understood. In this work, a divergent strategy is developed to synthesize a comprehensive library of 32 PNAG pentasaccharides. This library enables the identification of PNAG sequences with specific patterns of free amines as epitopes for vaccines against Staphylococcus aureus (S. aureus), an important human pathogen. Active vaccination with the conjugate of discovered PNAG epitope with mutant bacteriophage Qβ as a vaccine carrier as well as passive vaccination with diluted rabbit antisera provides mice with near complete protection against infections by S. aureus including methicillin-resistant S. aureus (MRSA). Thus, the comprehensive PNAG pentasaccharide library is an exciting tool to empower the design of next generation vaccines

Inducing long lasting B cell and T cell immunity against multiple variants of SARS-CoV-2 through mutant bacteriophage Qβ – receptor binding domain conjugate

More than two years into the global pandemic, SARS-CoV-2 remains a significant threat to public health. Immunities acquired from infection or current vaccines fail to provide long term protection against subsequent infections, mainly due to their fast-waning nature and the emergence of variants of concerns (VOCs) such as Omicron. To overcome these limitations, SARS-CoV-2 Spike protein receptor binding domain (RBD)-based epitopes were investigated as conjugates with a powerful carrier, the mutant bacteriophage Qβ (mQβ). The epitope design was critical to eliciting potent antibody responses with the full length RBD being superior to peptide and glycopeptide antigens. The full length RBD with orientation-controlled conjugation with mQβ activated both humoral and cellular immune systems in vivo, inducing broad spectrum, persistent and comprehensive immune responses effective against multiple VOCs including Delta and Omicron variants, rendering it a promising vaccine candidate