Idiomas sem fronteiras: Ensino, formação e pesquisa em línguas adicionais

O projeto “Idiomas sem Fronteiras: Ensino, formação e pesquisa em Línguas Adicionais” nasceu como um ramo do programa governamental “Ciências sem Fronteiras”, com o intuito de aperfeiçoar o ensino das línguas adicionais e internacionalizar o ensino superior brasileiro. Assim, a ação de extensão tem como objetivo, estudar o programa Idiomas sem Fronteiras e buscar maneiras mais dinâmicas e efetivas para sua implementação ao ensino de idiomas, de acordo com as necessidades dos alunos e servidores da UNILA, oferecendo cursos presenciais e online de inglês, espanhol, alemão e português para estrangeiros, valorizando os princípios da UNILA de multilinguismo, interculturalidade e integração latinoamericana. Essa nova dinâmica de aprendizagem tem sido desenvolvida juntamente com os professores e os alunos dos cursos do Idiomas sem Fronteiras, através de maior divulgação dessas atividades, que tem aumentado o número de participantes para melhor eficiência no desenvolvimento e melhoria de uma pedagogia de acordo com os ideais da UNILA e da Tríplice Fronteira

Adipocyte PU.1 knockout promotes insulin sensitivity in HFD-fed obese mice.

Insulin resistance is a key feature of obesity and type 2 diabetes. PU.1 is a master transcription factor predominantly expressed in macrophages but after HFD feeding PU.1 expression is also significantly increased in adipocytes. We generated adipocyte specific PU.1 knockout mice using adiponectin cre to investigate the role of PU.1 in adipocyte biology, insulin and glucose homeostasis. In HFD-fed obese mice systemic glucose tolerance and insulin sensitivity were improved in PU.1 AKO mice and clamp studies indicated improvements in both adipose and liver insulin sensitivity. At the level of adipose tissue, macrophage infiltration and inflammation was decreased and glucose uptake was increased in PU.1 AKO mice compared with controls. While PU.1 deletion in adipocytes did not affect the gene expression of PPARg itself, we observed increased expression of PPARg target genes in eWAT from HFD fed PU.1 AKO mice compared with controls. Furthermore, we observed decreased phosphorylation at serine 273 in PU.1 AKO mice compared with fl/fl controls, indicating that PPARg is more active when PU.1 expression is reduced in adipocytes. Therefore, in obesity the increased expression of PU.1 in adipocytes modifies the adipocyte PPARg cistrome resulting in impaired glucose tolerance and insulin sensitivity

Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis

Neck ventroflexion in cats has different causes; however, the most common is the hypokalemia associated with flaccid paralysis secondary to chronic renal failure. in humans, the most common causes of acute flaccid paralysis are hypokalemia precipitated by thyrotoxicosis and familial forms linked to mutations in sodium, potassium, and calcium channel genes. Here, we describe the sequencing and analysis of skeletal muscle ion channels in Felis catus that could be related to periodic paralyses in humans, contributing to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis. We studied genomic DNA from eleven cats, including five animals that were hyperthyroid with hypokalemia, although only one presented with muscle weakness, and six healthy control domestic cats. We identified the ion channel ortholog genes KCNJ2, KCNJ12, KCNJ14, CACNA1S and SCN4A in the Felis catus genome, together with several polymorphic variants. Upon comparative alignment with other genomes, we found that Felis catus provides evidence for a high genomic conservation of ion channel sequences. Although we hypothesized that neck ventroflexion in cats could be associated with a thyrotoxic or familial periodic paralysis channel mutation, we did not identify any previously detected human channel mutation in the hyperthyroid cat presenting hypokalemia. However, based on the small number of affected cats in this study, we cannot yet rule out this molecular mechanism. Notwithstanding, hyperthyroidism should still be considered as a differential diagnosis in hypokalemic feline paralysis.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Federal de São Paulo, Escola Paulista Med, Dept Med, Div Endocrinol,Lab Mol & Translat Endocrinol, BR-04039032 São Paulo, BrazilUniversidade Federal de São Paulo, Escola Paulista Med, Dept Med, Div Endocrinol,Lab Mol & Translat Endocrinol, BR-04039032 São Paulo, BrazilFAPESP: 2011/20747-8FAPESP: 2012/02529Web of Scienc

Probing the role of grain boundaries in single Cu nanoparticle oxidation by in situ plasmonic scattering

Grain boundaries determine physical properties of bulk materials including ductility, diffusivity, and electrical conductivity. However, the role of grain boundaries in nanostructures and nanoparticles is much less understood, despite the wide application of nanoparticles in nanophotonics, nanoelectronics, and heterogeneous catalysis. Here, we investigate the role of high-angle grain boundaries in the oxidation of Cu nanoparticles, using a combination of in situ single particle plasmonic nanoimaging and postmortem transmission electron microscopy image analysis, together with ab initio and classical electromagnetic calculations. We find an initial growth of a 5-nm-thick Cu2O shell on all nanoparticles, irrespective of different grain morphologies. This insensitivity of the Cu2O shell on the grain morphology is rationalized by extraction of Cu atoms from the metal lattice being the rate limiting step, as proposed by density functional theory calculations. Furthermore, we find that the change in optical scattering intensity measured from the individual particles can be deconvoluted into one contribution from the oxide layer growth and one contribution that is directly proportional to the grain boundary density. The latter contribution signals accumulation of Cu vacancies at the grain boundaries, which, as corroborated by calculations of the optical scattering, leads to increased absorption losses and thus a decrease of the scattering, thereby manifesting the role of grain boundaries as vacancy sinks and nuclei for Kirkendall void formation at a later stage of the oxidation process

Estudo comparativo de duas técnicas farmacopéicas de avaliação da atividade antimicrobiana dos fármacos: nistatina, eritromicina, neomicina e gentamicina

Through the diffusion techniques in culture media with paper disc and stainless steel cylinders the antimicrobial activity in 123 pharmaceutical preparations was evaluated: nystatin (43 samples of vaginal cream), erythromycin (14 samples of tablets and 9 of oral suspension), gentamicin (33 samples of injectable liquid) and neomycin (24 samples of cream of topic use), against the microorganisms: Saccharomyces cerevisiae ATCC 2601 to nystatin; Staphylococcus epidermidis12228 to gentamicin and neomycin, and Micrococcus luteusATCC 9341 to erythromycin. The preparation of the test organisms, standard solution, test solutions and culture media in both methods followed the recomendations of Brazilian Pharmacopoeia IV (1988) and United States Pharmacopoeia 24 (2000). The results was statically analysed by tests of Fisher (p=0,05) and Student (p=0,05). Among the 43 samples of vaginal cream nystatin analysed, checked out by the Student's test, differences of antimicrobial activity between methods of disc and cylinder were observed. However, the Fisher's test does not presented difference. The samples of erythromycin, gentamicin and neomycin did not show any difference when submitted to Fisher and Student tests in conclusion, to the erythromycin, gentamicin, neomycin and nystatin, the analysis of antimicrobial activity by the technique of paper discs can substitute the technique of the cylinders of stainless steel.Através das técnicas de difusão em ágar com discos de papel e cilindros de aço inoxidável, analisou-se quantitativamente a atividade antimicrobiana de 123 medicamentos contendo os fármacos: nistatina (43 amostras de creme vaginal), eritromicina (14 amostras de comprimidos e 9 amostras de suspensão oral), gentamicina (33 amostras de líquido injetável) e neomicina (24 amostras de creme de uso tópico), mediante as seguintes cepas de microrganismos: Saccharomyces cerevisiae ATCC 2601 para a nistatina; Staphylococcus epidermidis 12228 para a gentamicina e neomicina e Micrococcus luteus ATCC 9341 para a eritromicina. O preparo das cepas, soluções de padrão e amostra e meios de cultura nos dois métodos seguiram as recomendações da Farmacopéia Brasileira IV (1988) e Farmacopéia Americana 24 (2000). Os resultados foram analisados estatisticamente pelos testes de Fisher (p=0,05) e Student (p=0,05). Dentre as 43 amostras de nistatina creme vaginal analisadas, verificaram-se pelo teste de Student , diferenças pouco significativas (para p=0,05) de atividade antimicrobiana entre os métodos dos discos e cilindros; porém no teste de Fisher não houve diferenças. Nas amostras de eritromicina, gentamicina e neomicina não se obtiveram diferenças significativas entre os testes estatísticos de Fisher e Student. Conclui-se que, para a eritromicina, gentamicina, neomicina e nistatina, a análise de atividade antimicrobiana pela técnica dos discos de papel pode substituir a técnica dos cilindros de aço inoxidável

Startup de ergonomia como solução para o teletrabalho na pandemia

Mudanças econômicas, sociais e de formas de trabalho incentivam os novos desafios em busca de soluções em modelos de negócios disruptivos. Particularmente, a partir de 2020 houve a necessidade de desenvolver um modelo de negócio inovador, para atender a uma demanda ergonômica, ocasionada pela pandemia do Covid-19. Segundo a ASSOCIAÇÃO BRASILEIRA DE STARTUPS - ABS (2022), é preciso ser cada vez mais rápido, conectado e atualizado para atender as demandas do mercado. O objetivo deste trabalho foi sistematizar as etapas de uma startup de ergonomia como solução para atender online e de modo escalonável, pessoas em teletrabalho. As etapas foram: idealização, validação / produto mínimo viável, operação e tração. Os resultados foram a própria sistematização das etapas de uma Startup a qual permitiu concluir que é possível considerar um modelo de negócio inovador na área de ergonomia e que este tipo de empresa deve ser melhor explorado em estudos que venham a comprovar resultados relacionados a qualidade de vida, conforto e segurança no teletrabalho

Isolation of Oropouche Virus from Febrile Patient, Ecuador.

We report identification of an Oropouche virus strain in a febrile patient from Ecuador by using metagenomic sequencing and real-time reverse transcription PCR. Virus was isolated from patient serum by using Vero cells. Phylogenetic analysis of the whole-genome sequence showed the virus to be similar to a strain from Peru

Exclusão Digital de Gênero: quebrando o silêncio na Ciência da Informação

Latin America does not research on the problems of digital divide related to gender. This was one of the findings in the research made at Universidade Estadual Paulista, which included the most cited literature in English, Spanish and Portuguese in the domain of the digital divide. Women's lack of access to digital information technology or their inability to benefit from it, due to their gender, define a type of exclusion that is being studied in developed countries, but Latin America is neglected. To contribute to the development of gender research in librarian and information sciences, this study compiled the most relevant works about gender digital exclusion, identifying the main problems they refer to, articulating them in a conceptual map that allows to perceive the challenges and opportunities of research that they imply to the region. The target is to break academic silence in librarian and information sciences, and spread out what we know about gender digital exclusion, articulating it comprehensively to boost new projects, valuing female exclusion in science and technology, and specifically in information and communication technology, as a central problem of the research agenda of librarian and information sciences, of all Social Sciences, and of Latin American education and research institutions

Exclusão Digital de Gênero: quebrando o silêncio na Ciência da Informação

Latin America does not research on the problems of digital divide related to gender. This was one of the findings in the research made at Universidade Estadual Paulista, which included the most cited literature in English, Spanish and Portuguese in the domain of the digital divide. Women's lack of access to digital information technology or their inability to benefit from it, due to their gender, define a type of exclusion that is being studied in developed countries, but Latin America is neglected. To contribute to the development of gender research in librarian and information sciences, this study compiled the most relevant works about gender digital exclusion, identifying the main problems they refer to, articulating them in a conceptual map that allows to perceive the challenges and opportunities of research that they imply to the region. The target is to break academic silence in librarian and information sciences, and spread out what we know about gender digital exclusion, articulating it comprehensively to boost new projects, valuing female exclusion in science and technology, and specifically in information and communication technology, as a central problem of the research agenda of librarian and information sciences, of all Social Sciences, and of Latin American education and research institutions