A new method for the spectroscopic identification of stellar non-radial pulsation modes. II. Mode identification of the Delta Scuti star FG Virginis

We present a mode identification based on new high-resolution time-series spectra of the non-radially pulsating Delta Scuti star FG~Vir (HD 106384, V = 6.57, A5V). From 2002 February to June a global Delta Scuti Network (DSN) campaign, utilizing high-resolution spectroscopy and simultaneous photometry has been conducted for FG~Vir in order to provide a theoretical pulsation model. In this campaign we have acquired 969 Echelle spectra covering 147 hours at six observatories. The mode identification was carried out by analyzing line profile variations by means of the Fourier parameter fit method, where the observational Fourier parameters across the line are fitted with theoretical values. This method is especially well suited for determining the azimuthal order m of non-radial pulsation modes and thus complementary with the method of Daszynska-Daszkiewicz (2002) which does best at identifying the degree l. 15 frequencies between 9.2 and 33.5 c/d were detected spectroscopically. We determined the azimuthal order m of 12 modes and constrained their harmonic degree l. Only modes of low degree (l <= 4) were detected, most of them having axisymmetric character mainly due to the relatively low projected rotational velocity of FG Vir. The detected non-axisymmetric modes have azimuthal orders between -2 and 1. We derived an inclination of 19 degrees, which implies an equatorial rotational rate of 66 km/s.Comment: 14 pages, 26 figure

An asteroseismic study of the beta Cephei star 12 Lacertae: multisite spectroscopic observations, mode identification and seismic modelling

We present the results of a spectroscopic multisite campaign for the beta Cephei star 12 (DD) Lacertae. Our study is based on more than thousand high-resolution high S/N spectra gathered with 8 different telescopes in a time span of 11 months. In addition we make use of numerous archival spectroscopic measurements. We confirm 10 independent frequencies recently discovered from photometry, as well as harmonics and combination frequencies. In particular, the SPB-like g-mode with frequency 0.3428 1/d reported before is detected in our spectroscopy. We identify the four main modes as (l1,m1) = (1, 1), (l2,m2) = (0, 0), (l3,m3) = (1, 0) and (l4,m4) = (2, 1) for f1 = 5.178964 1/d, f2 = 5.334224 1/d, f3 = 5.066316 1/d and f4 = 5.490133 1/d, respectively. Our seismic modelling shows that f2 is likely the radial first overtone and that the core overshooting parameter alpha_ov is lower than 0.4 local pressure scale heights.Comment: 16 pages, 11 figures, accepted in MNRA

PHOEBE 2.0 – Where no model has gone before

phoebe 2.0 is an open source framework bridging the gap between stellar observations and models. It allows to create and fit models simultaneously and consistently to a wide range of observational data such as photometry, spectroscopy, spectrapolarimetry, interferometry and astrometry. To reach the level of precision required by the newest generation of instruments such as Kepler, GAIA and the arrays of large telescopes, the code is set up to handle a wide range of phenomena such as multiplicity, rotation, pulsations and magnetic fields, and to model the involved physics to a new level

Subcellular heterogeneity of ryanodine receptor properties in ventricular myocytes with low T-tubule density

Rationale: In ventricular myocytes of large mammals, not all ryanodine receptor (RyR) clusters are associated with T-tubules (TTs); this fraction increases with cellular remodeling after myocardial infarction (MI). Objective: To characterize RyR functional properties in relation to TT proximity, at baseline and after MI. Methods: Myocytes were isolated from left ventricle of healthy pigs (CTRL) or from the area adjacent to a myocardial infarction (MI). Ca2+ transients were measured under whole-cell voltage clamp during confocal linescan imaging (fluo-3) and segmented according to proximity of TTs (sites of early Ca2+ release, F&gt;F50 within 20 ms) or their absence (delayed areas). Spontaneous Ca2+ release events during diastole, Ca2+ sparks, reflecting RyR activity and properties, were subsequently assigned to either category. Results: In CTRL, spark frequency was higher in proximity of TTs, but spark duration was significantly shorter. Block of Na+/Ca2+ exchanger (NCX) prolonged spark duration selectively near TTs, while block of Ca2+ influx via Ca2+ channels did not affect sparks properties. In MI, total spark mass was increased in line with higher SR Ca2+ content. Extremely long sparks (&gt;47.6 ms) occurred more frequently. The fraction of near-TT sparks was reduced; frequency increased mainly in delayed sites. Increased duration was seen in near-TT sparks only; Ca2+ removal by NCX at the membrane was significantly lower in MI. Conclusion: TT proximity modulates RyR cluster properties resulting in intracellular heterogeneity of diastolic spark activity. Remodeling in the area adjacent to MI differentially affects these RyR subpopulations. Reduction of the number of sparks near TTs and reduced local NCX removal limit cellular Ca2+ loss and raise SR Ca2+ content, but may promote Ca2+ waves

Asteroseismology with the WIRE satellite. I. Combining Ground- and Space-based Photometry of the Delta Scuti Star Epsilon Cephei

We have analysed ground-based multi-colour Stromgren photometry and single-filter photometry from the star tracker on the WIRE satellite of the delta scuti star Epsilon Cephei. The ground-based data set consists of 16 nights of data collected over 164 days, while the satellite data are nearly continuous coverage of the star during 14 days. The spectral window and noise level of the satellite data are superior to the ground-based data and this data set is used to locate the frequencies. However, we can use the ground-based data to improve the accuracy of the frequencies due to the much longer time baseline. We detect 26 oscillation frequencies in the WIRE data set, but only some of these can be seen clearly in the ground-based data. We have used the multi-colour ground-based photometry to determine amplitude and phase differences in the Stromgren b-y colour and the y filter in an attempt to identify the radial degree of the oscillation frequencies. We conclude that the accuracies of the amplitudes and phases are not sufficient to constrain theoretical models of Epsilon Cephei. We find no evidence for rotational splitting or the large separation among the frequencies detected in the WIRE data set. To be able to identify oscillation frequencies in delta scuti stars with the method we have applied, it is crucial to obtain more complete coverage from multi-site campaigns with a long time baseline and in multiple filters. This is important when planning photometric and spectroscopic ground-based support for future satellite missions like COROT and KEPLER.Comment: 13 pages, 12 figures, 4 tables. Fig. 4 reduced in quality. Accepted by A&

Detection of gravity modes in the massive binary V380 Cyg from Kepler spacebased photometry and high-resolution spectroscopy

We report the discovery of low-amplitude gravity-mode oscillations in the massive binary star V380 Cyg, from 180 d of Kepler custom-aperture space photometry and 5 months of high-resolution high signal-to-noise spectroscopy. The new data are of unprecedented quality and allowed to improve the orbital and fundamental parameters for this binary. The orbital solution was subtracted from the photometric data and led to the detection of periodic intrinsic variability with frequencies of which some are multiples of the orbital frequency and others are not. Spectral disentangling allowed the detection of line-profile variability in the primary. With our discovery of intrinsic variability interpreted as gravity mode oscillations, V380 Cyg becomes an important laboratory for future seismic tuning of the near-core physics in massive B-type stars.Comment: 5 pages, 4 figures, 2 tables. Accepted for publication in MNRAS Letter

Human Galectins Induce Conversion of Dermal Fibroblasts into Myofibroblasts and Production of Extracellular Matrix: Potential Application in Tissue Engineering and Wound Repair

Members of the galectin family of endogenous lectins are potent adhesion/growth-regulatory effectors. Their multi-functionality opens possibilities for their use in bioapplications. We studied whether human galectins induce the conversion of human dermal fibroblasts into myofibroblasts (MFBs) and the production of a bioactive extracellular matrix scaffold is suitable for cell culture. Testing a panel of galectins of all three subgroups, including natural and engineered variants, we detected activity for the proto-type galectin-1 and galectin-7, the chimera-type galectin-3 and the tandem-repeat-type galectin-4. The activity of galectin-1 required the integrity of the carbohydrate recognition domain. It was independent of the presence of TGF-beta 1, but it yielded an additive effect. The resulting MFBs, relevant, for example, for tumor progression, generated a matrix scaffold rich in fibronectin and galectin-1 that supported keratinocyte culture without feeder cells. Of note, keratinocytes cultured on this substratum presented a stem-like cell phenotype with small size and keratin-19 expression. In vivo in rats, galectin-1 had a positive effect on skin wound closure 21 days after surgery. In conclusion, we describe the differential potential of certain human galectins to induce the conversion of dermal fibroblasts into MFBs and the generation of a bioactive cell culture substratum. Copyright (C) 2011 S. Karger AG, Base

Some Aspects of Cost/ Benefit Analysis for In-Service Inspection of PWR Steam Generators

This report discusses a number of aspects of cost/benefit (C/B) analysis for in-service inspection (lSI} of pressurized water reactor (PWR) steam generators (SGs) and identifies several problem areas that must be addressed prior to a full C/B analysis capability. Following a brief review of the impact of SG problems on the productivity of PWR units and of the scope and variability of SG problems among U.S. PWRs, various occupational implications of SG lSI are considered, namely manpower, time, and rad exposure. The opportunities provided by refueling outages in respect to lSI frequency and work time windows are reviewed. Indices for characterizing the nondestructive testing {NDT) information, rad exposure, $impact, and manpower and time attributes of single ISIs and a series of ISIs over an arbitrary evaluation period are presented and calculated for a number of lSI cases using SG parameters for three typical PWR units. A comparison of the$ impact of unscheduled outages attributable to SG problems with the $cost of ambitious lSI strategies indicates that the$ cost is virtually negligible for well-planned ISis. Considering the ALARA constraint on occupational rad exposure, the skilled manpower pool for NDT work appears to be the principal factor limiting lSI scope and frequency. Analysis of the manpower and time requirements for inspection of a 40-unit PWR population indicates, however, that an lSI strategy embodying two campaigns per year and a total population inspection within a 2-year interval is not far beyond current capabilities

Synthetic pulsational line profile variations

We have produced simulated time series of high resolution spectroscopy for sdBs. We present the first results of testing classical mode identification techniques. Here, in particular, we analyse the behaviour of individual spectral lines and discuss the results and applicability of the moment method.Peer reviewe

Post-AGB stars with hot circumstellar dust: binarity of the low-amplitude pulsators

While the first binary post-AGB stars were serendipitously discovered, the distinct characteristics of their Spectral Energy Distribution (SED) allowed us to launch a more systematic search for binaries. We selected post-AGB objects which show a broad dust excess often starting already at H or K, pointing to the presence of a gravitationally bound dusty disc in the system. We started a very extensive multi-wavelength study of those systems and here we report on our radial velocity and photometric monitoring results for six stars of early F type, which are pulsators of small amplitude. To determine the radial velocity of low signal-to-noise time-series, we constructed dedicated auto-correlation masks. The radial velocity variations were subjected to detailed analysis to differentiate between pulsational variability and variability due to orbital motion. Finally orbital minimalisation was performed to constrain the orbital elements. All of the six objects are binaries, with orbital periods ranging from 120 to 1800 days. Five systems have non-circular orbits. The mass functions range from 0.004 to 0.57 solar mass and the companions are likely unevolved objects of (very) low initial mass. We argue that these binaries must have been subject to severe binary interaction when the primary was a cool supergiant. Although the origin of the circumstellar disc is not well understood, the disc is generally believed to be formed during this strong interaction phase. The eccentric orbits of these highly evolved objects remain poorly understood. With the measured orbits and mass functions we conclude that the circumbinary discs seem to have a major impact on the evolution of a significant fraction of binary systems.Comment: 13 pages, 15 figures, accepted for Astronomy and Astrophysic