Effect of the Incorporation of Titanium on the Optical Properties of ZnO Thin Films: From Doping to Mixed Oxide Formation

ZnO films with Ti atoms incorporated (TZO) in a wide range (0–18 at.%) have been grown by reactive co-sputtering on silicon and glass substrates. The influence of the titanium incorporation in the ZnO matrix on the structural and optical characteristics of the samples has been determined by Rutherford backscattering spectroscopy (RBS), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD). The results indicate that the samples with low Ti content (<4 at.%) exhibit a wurtzite-like structure, with the Ti4+ ions substitutionally incorporated into the ZnO structure, forming Ti-doped ZnO films. In particular, a very low concentration of Ti (<0.9 at.%) leads to a significant increase of the crystallinity of the TZO samples. Higher Ti contents give rise to a progressive amorphization of the wurtzite-like structure, so samples with high Ti content ( 18 at.%) display an amorphous structure, indicating in the XPS analysis, a predominance of Ti–O–Zn mixed oxides. The energy gap obtained from absorption spectrophotometry increases from 3.2 eV for pure ZnO films to 3.6 eV for those with the highest Ti content. Ti incorporation in the ZnO samples <0.9 at.% raises both the blue (380 nm) and green (approx. 550 nm) bands of the photoluminescence (PL) emission, thereby indicating a significant improvement of the PL efficiency of the samples

New Motherhood Concepts, Implications for Healthcare. A Qualitative Study Article

11 p.The aim of this study was to explore the experience of women who take care of their children in postpartum and who desire to be understood by society, with no judgements. For this purpose, a qualitative methodology was followed. In-depth interviews, discussion groups, and an online forum were used for data collection. The participants were Spanish women that had given birth in the past 6 months, and their partners. Healthcare specialists with experience in the topic were also included. Results showed three main categories: lack of priority, self-demand, and self-esteem changes. As a conclusion, the concept of motherhood needs to be redefined, as women feel that they are living under the pressure of being a "perfect mother". It is important that mothers allow themselves to fail in reaching the imposed requirements. Simple acceptance of motherhood boundaries could help in this transition

Resistance to 2-Hydroxy-Flutamide in Prostate Cancer Cells Is Associated with the Downregulation of Phosphatidylcholine Biosynthesis and Epigenetic Modifications

18 p.In this study, we examined the metabolic adaptations of a chemoresistant prostate cancer cell line in comparison to a sensitive cell line. We utilized prostate cancer LNCaP cells and subjected them to a stepwise increase in the antiandrogen 2-hydroxy-flutamide (FLU) concentration to generate a FLU-resistant cell line (LN-FLU). These LN-FLU cells displayed characteristics of cancer stem cells, exhibited drug resistance, and showed a significantly reduced expression of Cyclin D1, along with the overexpression of p16, pointing to a proliferation arrest. In comparing the cancer stem-like LN-FLU cells to the LNCaP cells, we observed a decrease in the expression of CTP-choline cytidylyl transferase ? (CCT?), as well as a decline in choline kinase, suggesting altogether a downregulation of the phosphatidylcholine biosynthetic pathway. In addition, we found decreased levels of the protein methyl transferase PRMT2 and the upregulation of the histone deacetylase Sirtuin1 (Sirt1). Analysis of the human prostate cancer samples revealed similar results in a population with high expressions of the stem cell markers Oct4 and ABCB1A1. Our findings suggest that the adaptation of prostate cancer cells to antiandrogens could induce reprogramming into stem cells that survive in a low phosphocholine metabolism and cell cycle arrest and display drug resistance.Instituto de Salud Carlos IIFundación Tatiana Pérez de Guzmán el BuenoComunidad de Madri

An Acoustic Modem Featuring a Multi-Receiver and Ultra-Low Power

[EN] Wireless technology for underwater communication possesses a wide range of potential application, but it is still a relatively unexplored area in many aspects concerning modems physical design. A step towards future deployment of underwater networks is the reduction of power consumption. Therefore, asynchronous wakeup systems need to be integrated within the physical layer design while avoiding the use of additional transducers. This paper offers a practical and generic solution to adapt data reception and transmission together with asynchronous wakeup sub-systems in acoustic underwater modem architectures using a low power and low cost solution. The proposal has been implemented in a real prototype with success.The translation of this paper was funded by the Universitat Politècnica de València, Spain.The authors gratefully acknowledge financial support from the CICYT. ANDREA: Automated Inspection and Remote Performance of Marine Fish Farms (CTM2011-29691-C02-01) and RIDeWAN: Research on Improvement of the Dependability of WSN-based Applications by Developing a Hybrid Monitoring Platform. (TIN2011-28435-C03-01).Sánchez Matías, AM.; Blanc Clavero, S.; Yuste Pérez, P.; Perles Ivars, A.; Serrano Martín, JJ. (2015). An Acoustic Modem Featuring a Multi-Receiver and Ultra-Low Power. Circuits and Systems. 6(1):1-12. https://doi.org/10.4236/cs.2015.61001S1126

Escape room inverso como metodología motivadora en la enseñanza de transmisión de calor

En este trabajo presentamos nuestra experiencia con el proyecto “Yo sé resolver problemas con ecuaciones” cuya primera iteración recibió el premio a la mejor experiencia innovadora en secundaria y bachillerato en SIMO 2019. El proyecto persigue dos objetivos: propiciar el aprendizaje de resolución de problemas y reducir el nivel de ansiedad que genera enfrentarse a problemas de matemáticas. Para conseguirlo, los alumnos deben grabarse resolviendo y explicando problemas de matemáticas. Con la realización del proyecto observamos que permite evaluar aspectos del conocimiento, comprensión y habilidades que no son fáciles de observar con tareas más tradicionales. Adicionalmente, conseguimos un banco de problemas resueltos que crece año a año con vídeos creados por los alumnos. De modo que los alumnos pueden aprender de sus compañeros de cursos superiores. En este trabajo, también aportamos justificaciones pedagógicas y orientaciones didácticas para llevar a cabo el proyecto, junto a las rúbricas de evaluación

Advanced Acoustic Wake-Up System for Underwater Sensor Networks

[EN] This paper presents a low-cost and low-power consumption asynchronous Wake-Up (WU) development to Underwater Wireless Sensor Networks (UWSN). An asynchronous WU offers important advantages for energyaware network polices, however it needs some specific hardware, an optimal configuration of system facilities and the interconnection with a core control unit. This proposed WU implementation has been specifically designed to be used in acoustic underwater modems, able to react to external acoustic stimuli. Both the modem and the Wake-Up system use a unique piezoelectric transducer dissipating, to our knowledge, the lowest power published until now. Moreover, the system is able to detect both simple tones and predefined bit patterns, being able to wake up a network node UWSN individually or even to different nodes at the same time.This work was supported in part by the Spanish Government: Project funds of SABINA CTM2011-29691-C02-01 (SENSORIZACIÓN AMBIENTAL SUBACUÁTICA PARA LA INSPECCIÓN Y MONITORIZACIÓN DE EXPLOTACIONES DE ACUICULTURA MARIAN) AND RIDEWAN TIN2011-28435-C03-01 (INVESTIGACIÓN EN LA MEJORA DE LA CONFIABILIDAD DE APLICACIONES BASADAS EN WSN MEDIANTE EL DESARROLLO DE UNA PLATAFORMA HIBRIDA DE MONITORIZACIÓN)Sánchez Matías, AM.; Blanc Clavero, S.; Yuste Pérez, P.; Piqueras Gozalbes, IR.; Serrano Martín, JJ. (2012). Advanced Acoustic Wake-Up System for Underwater Sensor Networks. Communications in information science and management engineering. 2(2):1-10. http://hdl.handle.net/10251/36744S1102

Diabetic individuals with COVID-19 exhibit reduced efficacy of gliptins in inhibiting dipeptidyl peptidase 4 (DPP4). A suggested explanation for increased COVID-19 susceptibility in patients with type 2 diabetes mellitus (T2DM)

Aims: Dipeptidyl peptidase 4 (DPP4) has been proposed as a coreceptor for SARS-CoV-2 cellular entry. Considering that type 2 diabetes mellitus (T2DM) has been identified as the most important risk factor for SARS-CoV-2, and that gliptins (DPP4 inhibitors) are a prescribed diabetic treatment, this study aims to unravel the impact of DPP4 in the intersection of T2DM/COVID-19. Materials and methods: We analyzed 189 serum human samples, divided into six clinical groups (controls, T2DM, T2DM + gliptins, COVID-19, COVID-19 + T2DM, and COVID-19 + T2DM + gliptins), measuring DPP4 protein concentration and activity by Western blot, ELISA, and commercial activity kits. The obtained results were verified in Huh-7 cellular models. Key findings: Both DPP4 concentration and activity were decreased in COVID-19 patients, and as in T2DM patients, compared to controls. Despite these lower levels, the ratio of DPP4 activity/concentration in COVID-19 sera was the highest (0.782 ± 0.289 ?U/ng vs. 0.547 ± 0.050 ?U/ng in controls, p < 0.0001), suggesting a compensating mechanism in these patients. Supernatants of Huh-7 cells incubated with COVID-19 serum showed a consistent and significantly lower DPP4 concentration and activity. Furthermore, COVID-19 + T2DM + gliptins patients showed a higher serum DPP4 concentration and activity than T2DM + gliptin subjects (p < 0.05), indicating that sera from COVID-19 convalescents interfere with gliptins. Significance: Either SARS-CoV-2 or some metabolites present in the sera of COVID-19-convalescent patients interact with soluble DPP4 or even gliptins themselves since the inhibitory effect of gliptins on DPP4 activity is being prevented. The interactions between DPP4, gliptins, and SARS-CoV-2 should be further elucidated to reveal the mechanism of action for these interesting observations.Instituto de Salud Carlos IIIComunidad de MadridUniversidad de AlcaláFundación Tatiana Pérez de Guzmán el Buen

Overview of pneumococcal serotypes and genotypes causing diseases in patients with chronic obstructive pulmonary disease in a Spanish hospital between 2013 and 2016

Background: Streptococcus pneumoniae is an important pathogen in chronic obstructive pulmonary disease (COPD). We aimed at showing the recent changes in the epidemiology of pneumococcal diseases in patients with COPD, especially after the introduction of the 13-valent pneumococcal conjugate vaccine (PCV13). Methods: From 2013 to 2016, strains causing invasive pneumococcal disease (IPD), non-bacteremic pneumococcal pneumonia (non-BPP), and acute exacerbation of COPD (AE-COPD) were prospectively included. Antimicrobial susceptibility testing, serotyping, and genotyping were analyzed. Results: We collected 345 pneumococci from 286 COPD patients (57 IPD, 78 non-BPP, and 210 AE-COPD). The most frequent serotypes were serotypes 3 (14.0%), 8 (14.0%), and 12F (8.8%) in IPD; serotypes 3 (16.7%), 11A (9%), 9L/N (7.7%), and 23A (7.7%) in non-BPP; and serotypes 11A (11%), nontypeable (11%), and 6C (10%) in AE-COPD. Resistance rates were highest among AE-COPD strains. Penicillin resistance was associated with serotypes 11A (CC156) and 19A (CC320 and CC230). Compared with previous studies, fluoroquinolone resistance in AE-COPD increased (9.5%), PCV13 serotypes decreased (31.6%, 26.9%, and 16.7% for IPD, non-BPP, and AE-COPD, respectively), and serotype 11A-CC156 in AE-COPD and serotype 8 in IPD increased. Conclusion: The epidemiology of pneumococcal disease in COPD changed after the introduction of PCV13 in children. Increases in the highly invasive serotype 8 among patients with IPD and in serotype 11A-CC156 among patients with AE-COPD could compromise the ability of current PCVs to prevent diseases. Vaccines with a greater coverage could improve the benefits of adult vaccination

Extracellular Vesicles Enriched in Connexin 43 Promote a Senescent Phenotype in Bone and Synovial Cells Contributing to Osteoarthritis Progression

[Abstract] The accumulation of senescent cells is a key characteristic of aging, leading to the progression of age-related diseases such as osteoarthritis (OA). Previous data from our laboratory has demonstrated that high levels of the transmembrane protein connexin 43 (Cx43) are associated with a senescent phenotype in chondrocytes from osteoarthritic cartilage. OA has been reclassified as a musculoskeletal disease characterized by the breakdown of the articular cartilage affecting the whole joint, subchondral bone, synovium, ligaments, tendons and muscles. However, the mechanisms that contribute to the spread of pathogenic factors throughout the joint tissues are still unknown. Here, we show for the first time that small extracellular vesicles (sEVs) released by human OA-derived chondrocytes contain high levels of Cx43 and induce a senescent phenotype in targeted chondrocytes, synovial and bone cells contributing to the formation of an inflammatory and degenerative joint environment by the secretion of senescence-associated secretory associated phenotype (SASP) molecules, including IL-1ß and IL-6 and MMPs. The enrichment of Cx43 changes the protein profile and activity of the secreted sEVs. Our results indicate a dual role for sEVs containing Cx43 inducing senescence and activating cellular plasticity in target cells mediated by NF-kß and the extracellular signal-regulated kinase 1/2 (ERK1/2), inducing epithelial-to-mesenchymal transition (EMT) signalling programme and contributing to the loss of the fully differentiated phenotype. Our results demonstrated that Cx43-sEVs released by OA-derived chondrocytes spread senescence, inflammation and reprogramming factors involved in wound healing failure to neighbouring tissues, contributing to the progression of the disease among cartilage, synovium, and bone and probably from one joint to another. These results highlight the importance for future studies to consider sEVs positive for Cx43 as a new biomarker of disease progression and new target to treat OA.This work was supported in part through funding from Health Institute ‘Carlos III’ (ISCIII, Spain), the European Regional Development Fund, ‘A way of making Europe’ from the European Union (to MDM): grant PI19/00145; a grant from the Joint Transnational Call for Proposals for “European Innovative Research & Technological Development Projects in Nanomedicine” EURONANOMED III (AC21_2/00026) (to MDM); a grant from Xunta de Galicia (IN607B2020/12) (to MDM) and from H2020, Future and Emerging Technologies (grant 858014 “PANACHE”) to MDM. MV-E was funded with a predoctoral (ED481A-2015/188) and post-doctoral fellowship (IN606B-2019/004) from Xunta de Galicia. AG-C was funded with a predoctoral fellowship (FIS20/00310) from ISCIII. PC-F was funded with a post-doctoral fellowship and a grant from Xunta de Galicia (INB606B 2017/014 and IN606C 2021/006). We thank members of the CellCOM group for helpful technical suggestion, María Dolores Álvarez Alvariño (CHUS) for generously collecting tissue samples in the operating room after surgery and Arantxa Tabernero (INCYL, University of Salamanca) for kindly providing the human Cx43 plasmid used in this studyXunta de Galicia; IN607B2020/12Xunta de Galicia; ED481A-2015/188Xunta de Galicia; IN606B-2019/004Xunta de Galicia; INB606B 2017/014Xunta de Galicia; IN606C 2021/00

Resistencia a la insulina y Enfermedad de Alzheimer prodrómica en la Comunidad de La Rioja: primeras impresiones

Trabajo presentado en la XLIII Reunión de la Sociedad de Neurología del País Vasco, celebrada en Vitoria (España), el 6 de mayo de 202