Central and Enteric Neuroprotective Effects by Eucommia ulmoides Extracts on Neurodegeneration in Rotenone-induced Parkinsonian Mouse

Parkinson’s disease (PD) is a progressive neurodegenerative disease of both the central and peripheral / enteric nervous systems. Oxidative stress and neuroinflammation are associated with the pathogenesis of PD, suggesting that anti-oxidative and anti-inflammatory compounds could be neuroprotective agents for PD. Eucommia ulmoides (EU) is a traditional herbal medicine which exerts neuroprotective effects by anti-inflammatory and anti-oxidative properties. Our previous study showed that treatment with chlorogenic acid, a component of EU, protected against neurodegeneration in the central and enteric nervous systems in a PD model. In this study, we examined the effects of EU extract (EUE) administration on dopaminergic neurodegeneration, glial response and α-synuclein expression in the substantia nigra pars compacta (SNpc), and intestinal enteric neurodegeneration in low-dose rotenone-induced PD model mice. Daily oral administration of EUE ameliorated dopaminergic neurodegeneration and α-synuclein accumulation in the SNpc. EUE treatment inhibited rotenone- induced decreases in the number of total astrocytes and in those expressing the antioxidant molecule metallothionein. EUE also prevented rotenone-induced microglial activation. Furthermore, EUE treatment exerted protective effects against intestinal neuronal loss in the PD model. These results suggest that EU exerts neuroprotective effects in the central and enteric nervous systems of rotenone-induced parkinsonism mice, in part by glial modification

Peptide barcoding for one-pot evaluation of sequence–function relationships of nanobodies

遊離型抗体の構造活性相関解析を迅速に評価可能とする新手法を開発. 京都大学プレスリリース. 2021-11-08.Optimisation of protein binders relies on laborious screening processes. Investigation of sequence–function relationships of protein binders is particularly slow, since mutants are purified and evaluated individually. Here we developed peptide barcoding, a high-throughput approach for accurate investigation of sequence–function relationships of hundreds of protein binders at once. Our approach is based on combining the generation of a mutagenised nanobody library fused with unique peptide barcodes, the formation of nanobody–antigen complexes at different ratios, their fine fractionation by size-exclusion chromatography and quantification of peptide barcodes by targeted proteomics. Applying peptide barcoding to an anti-GFP nanobody as a model, we successfully identified residues important for the binding affinity of anti-GFP nanobody at once. Peptide barcoding discriminated subtle changes in KD at the order of nM to sub-nM. Therefore, peptide barcoding is a powerful tool for engineering protein binders, enabling reliable one-pot evaluation of sequence–function relationships

Strange filamentary structures ("fireballs") around a merger galaxy in the Coma cluster of galaxies

We found an unusual complex of narrow blue filaments, bright blue knots, and H-alpha emitting filaments and clouds, which morphologically resembled a complex of ``fireballs,'' extending up to 80 kpc south from an E+A galaxy RB199 in the Coma cluster. The galaxy has a highly disturbed morphology indicative of a galaxy--galaxy merger remnant. The narrow blue filaments extend in straight shapes toward the south from the galaxy, and several bright blue knots are located at the southern ends of the filaments. The Rc band absolute magnitudes, half light radii and estimated masses of the bright knots are -12 - -13 mag, 200 - 300 pc and 10^6-7 Msolar, respectively. Long, narrow H-alpha emitting filaments are connected at the south edge of the knots. The average color of the fireballs is B - Rc = 0.5, which is bluer than RB199 (B - R = 0.99), suggesting that most of the stars in the fireballs were formed within several times 10^8 yr. The narrow blue filaments exhibit almost no H-alpha emission. Strong H-alpha and UV emission appear in the bright knots. These characteristics indicate that star formation recently ceased in the blue filaments and now continues in the bright knots. The gas stripped by some mechanism from the disk of RB199 may be traveling in the intergalactic space, forming stars left along its trajectory. The most plausible fireball formation mechanism is ram pressure stripping by high-speed collision between the galaxy and the hot intra-cluster medium. The fireballs may be a snapshot of diffuse intra-cluster population formation, or halo star population formation in a cluster galaxy.Comment: 13 pages, 14 figures, submitted to Ap

A star-bursting proto-cluster in making associated to a radio galaxy at z=2.53 discovered by H_alpha imaging

We report a discovery of a proto-cluster in vigorous assembly and hosting strong star forming activities, associated to a radio galaxy USS 1558-003 at z=2.53, as traced by a wide-field narrow-band H_alpha imaging with MOIRCS on Subaru Telescope. We find 68 H_alpha emitters with dust-uncorrected SFRs down to 8.6 Msun/yr. Their spatial distribution indicates that there are three prominent clumps of H_alpha emitters, one surrounding the radio galaxy and another located at ~1.5 Mpc away to the south-west, and the other located in between the two. These contiguous three systems are very likely to merge together in the near future and may grow to a single more massive cluster at later times. Whilst most H_alpha emitters reside in the "blue cloud" on the color--magnitude diagram, some emitters have very red colors with J-Ks>1.38(AB). Interestingly, such red H_alpha emitters are located towards the faint end of the red sequence, and they tend to be located in the high density clumps. We do not see any statistically significant difference in the distributions of individual star formation rates or stellar masses of the H_alpha emitters between the dense clumps and the other regions, suggesting that this is one of the notable sites where the progenitors of massive galaxies in the present-day clusters were in their vigorous formation phase. Finally, we find that H_alpha emission of the radio galaxy is fairly extended spatially over ~4.5 arcsec. However it is not as widespread as its Lya halo, meaning that the Lya emission is indeed severely extended by resonant scattering.Comment: 14 pages, 11 figures, 3 tables, accepted for publication in Ap

High star formation activity in the central region of a distant cluster at z=1.46

We present an unbiased deep [OII] emission survey of a cluster XMMXCS J2215.9-1738 at z=1.46, the most distant cluster to date with a detection of extended X-ray emission. With wide-field optical and near-infrared cameras (Suprime-Cam and MOIRCS, respectively) on Subaru telescope, we performed deep imaging with a narrow-band filter NB912 (lambda_c=9139A, Delta_lambda=134A) as well as broad-band filters (B, z', J and Ks). From the photometric catalogues, we have identified 44 [OII] emitters in the cluster central region of 6'x6' down to a dust-free star formation rate of 2.6 Msun/yr (3 sigma). Interestingly, it is found that there are many [OII] emitters even in the central high density region. In fact, the fraction of [OII] emitters to the cluster members as well as their star formation rates and equivalent widths stay almost constant with decreasing cluster-centric distance up to the cluster core. Unlike clusters at lower redshifts (z<1) where star formation activity is mostly quenched in their central regions, this higher redshift 2215 cluster shows its high star formation activity even at its centre, suggesting that we are beginning to enter the formation epoch of some galaxies in the cluster core eventually. Moreover, we find a deficit of galaxies on the red sequence at magnitudes fainter than ~M*+0.5 on the colour-magnitude diagram. This break magnitude is brighter than that of lower redshift clusters, and it is likely that we are seeing the formation phase of more massive red galaxies in the cluster core at z~1. These results may indicate inside-out and down-sizing propagation of star formation activity in the course of cluster evolution.Comment: 12 pages, 9 figures, 1 table, accepted for publication in MNRA

A large scale structure traced by [OII] emitters hosting a distant cluster at z=1.62

We present a panoramic narrow-band imaging survey of [OII] emitters in and around the ClG J0218.3-0510 cluster at z=1.62 with Suprime-Cam on Subaru telescope. 352 [OII] emitters were identified on the basis of narrow-band excesses and photometric redshifts. We discovered a huge filamentary structure with some clumps traced by [OII] emitters and found that the ClG J0218.3-0510 cluster is embedded in an even larger super-structure than the one reported previously. 31 [OII] emitters were spectroscopically confirmed with the detection of H-alpha and/or [OIII] emission lines by FMOS observations. In the high density regions such as cluster core and clumps, star-forming [OII] emitters show a high overdensity by a factor of more than 10 compared to the field region. Although the star formation activity is very high even in the cluster core, some massive quiescent galaxies also exits at the same time. Furthermore, the properties of the individual [OII] emitters, such as star formation rates, stellar masses and specific star formation rates, do not show a significant dependence on the local density, either. Such lack of environmental dependence is consistent with our earlier result by Hayashi et al. (2011) on a z=1.5 cluster and its surrounding region. The fact that the star-forming activity of galaxies in the cluster core is as high as that in the field at z~1.6 may suggest that the star-forming galaxies are probably just in a transition phase from a starburst mode to a quiescent mode, and are thus showing comparable level of star formation rates to those in lower density environments. We may be witnessing the start of the reversal of the local SFR--density relation due to the "biased" galaxy formation and evolution in high density regions at high this redshift, beyond which massive galaxies would be forming vigorously in a more biased way in proto-cluster cores.Comment: 11 pages, 9 figures, 1 table, accepted for publication in MNRA

Properties of star-forming galaxies in a cluster and its surrounding structure at z=1.46

We conduct a narrow-band imaging survey of [OII] emitters over a 32'x23' area in and around the XMMXCS J2215.9-1738 cluster at z=1.46 with Subaru/Suprime-Cam, and select 380 [OII] emitting galaxies down to 1.4E-17 erg/s/cm2. Among them, 16 [OII] emitters in the cluster central region are confirmed by NIR spectroscopy with Subaru/MOIRCS. We find that [OII] emitters are distributed along filamentary large-scale structures around the cluster. The z'-K vs K colour-magnitude diagram shows that a significantly higher fraction of [OII] emitters is seen on the red sequence in the cluster core than in other environments we define in this paper. It is likely that these red galaxies are nearly passively evolving galaxies which host [OII] emitting AGNs, rather than dust-reddened star-forming galaxies. We argue therefore that AGN feedback may be one of the critical processes to quench star formation in massive galaxies in high density regions. We also find that the cluster has experienced high star formation activities at rates comparable to that in the field at z=1.46. In addition, a mass-metallicity relation exists in the cluster at z=1.46, which is similar to that of star-forming galaxies in the field at z~2. These results all suggest that at z~1.5 star formation activity in the cluster core becomes as high as those in low density environments and there is apparently not yet a strong environmental dependence, except for the red emitters.Comment: 19 pages, 17 figures, 4 tables, accepted for publication in MNRA

Mapping dusty star formation in and around a cluster at z=0.81 by wide-field imaging with AKARI

We present environmental dependence of dusty star forming activity in and around the cluster RXJ1716.4+6708 at z=0.81 based on wide-field and multi-wavelength observations with Suprime-Cam on the Subaru telescope and IRC onboard the AKARI satellite. Our optical data shows that the optical colour distribution of galaxies starts to dramatically change from blue to red at the medium-density environment such as cluster outskirts, groups and filaments. By combining with infrared data, we find that 15 micron galaxies tend to have optical colours between the red sequence and the blue cloud with a tail into the red sequence. The spatial distribution of the 15 micron galaxies over ~200 arcmin^2 around the cluster reveals that few 15 micron galaxies are detected in the cluster central region. This is probably due to the low star forming activity in the cluster core. However, interestingly, the fraction of 15 micron galaxies in the medium-density environments is as high as in the low-density field, despite the fact that the optical colours start to change in the medium-density environments. Furthermore, we find that 15 micron galaxies which have optically red colours (candidates for dusty red galaxies) and galaxies with high specific star formation rates are also concentrated in the medium-density environment. These results imply that the star forming activity in galaxies in groups and filaments is enhanced due to some environmental effects specific to the medium-density environment, and such a phenomenon is probably directly connected to the truncation of star forming activity in galaxies seen as the dramatic change in optical colours in such environments.Comment: 15 pages, 14 figures, accepted for publication in MNRA