Consumer Preference on Traceable Information of Dairy Products

Diary industry in China has been in deep crisis since a series of quality scandals were exposed to public in 2008. Thanks to the traceability system in dairy supply chain and the growth of the internet, providing traceable dairy product information to the public is viewed as one of the best ways, mostly in terms of feasibility , to overcome the trust crisis and to promote the development of the dairy industry in China. However, among the tons of information available from the supply chain, there is a lack of knowledge on consumer preference. Based on choice-based conjoint analysis, this paper investigated consumer preference on dairy product traceable information query service. Specifically , this paper measured the value that consumers place on the dairy product traceable information. We used a multinomial logit model to estimate the preferences and offered 3 ways to explain the value of each kind of information that consumers are concerned about. Results indicate that different consumer groups hold different preferences

STP Models of Optimal Differential and Linear Trail for S-box Based Ciphers

Automatic tools have played an important role in designing new cryptographic primitives and evaluating the security of ciphers. Simple Theorem Prover constraint solver (STP) has been used to search for differential/linear trails of ciphers. This paper proposes general STP-based models searching for differential and linear trails with the optimal probability and correlation for S-box based ciphers. In order to get trails with the best probability or correlation for ciphers with arbitrary S-box, we give an efficient algorithm to describe probability or correlation of S-Box. Based on the algorithm we present a search model for optimal differential and linear trails, which is efficient for ciphers with S-Boxes whose DDTs/LATs contain entities not equal to the power of two. Meanwhile, the STP-based model for single-key impossible differentials considering key schedule is proposed, which traces the propagation of values from plaintext to ciphertext instead of propagations of differences. And we found that there is no 5-round AES-128 single-key truncated impossible differential considering key schedule, where input and output differences have only one active byte respectively. Finally, our proposed models are utilized to search for trails of bit-wise ciphers GIFT-128, DES, DESL and ICEBERG and word-wise ciphers ARIA, SM4 and SKINNY-128. As a result, improved results are presented in terms of the number of rounds or probabilities/correlations

Decoding the stoichiometric composition and organisation of bacterial metabolosomes

Some enteric bacteria including Salmonella have evolved the propanediol-utilising microcompartment (Pdu MCP), a specialised proteinaceous organelle that is essential for 1,2-propanediol degradation and enteric pathogenesis. Pdu MCPs are a family of bacterial microcompartments that are self-assembled from hundreds of proteins within the bacterial cytosol. Here, we seek a comprehensive understanding of the stoichiometric composition and organisation of Pdu MCPs. We obtain accurate stoichiometry of shell proteins and internal enzymes of the natural Pdu MCP by QconCAT-driven quantitative mass spectrometry. Genetic deletion of the major shell protein and absolute quantification reveal the stoichiometric and structural remodelling of metabolically functional Pdu MCPs. Decoding the precise protein stoichiometry allows us to develop an organisational model of the Pdu metabolosome. The structural insights into the Pdu MCP are critical for both delineating the general principles underlying bacterial organelle formation, structural robustness and function, and repurposing natural microcompartments using synthetic biology for biotechnological applications

Protein kinase A negatively regulates Ca2+ signalling in Toxoplasma gondii

The phylum Apicomplexa comprises a group of obligate intracellular parasites that alternate between intracellular replicating stages and actively motile extracellular forms that move through tissue. Parasite cytosolic Ca2+ signalling activates motility, but how this is switched off after invasion is complete to allow for replication to begin is not understood. Here, we show that the cyclic adenosine monophosphate (cAMP)-dependent protein kinase A catalytic subunit 1 (PKAc1) of Toxoplasma is responsible for suppression of Ca2+ signalling upon host cell invasion. We demonstrate that PKAc1 is sequestered to the parasite periphery by dual acylation of PKA regulatory subunit 1 (PKAr1). Upon genetic depletion of PKAc1 we show that newly invaded parasites exit host cells shortly thereafter, in a perforin-like protein 1 (PLP-1)-dependent fashion. Furthermore, we demonstrate that loss of PKAc1 prevents rapid down-regulation of cytosolic [Ca2+] levels shortly after invasion. We also provide evidence that loss of PKAc1 sensitises parasites to cyclic GMP (cGMP)-induced Ca2+ signalling, thus demonstrating a functional link between cAMP and these other signalling modalities. Together, this work provides a new paradigm in understanding how Toxoplasma and related apicomplexan parasites regulate infectivity

Synthetic engineering of a new biocatalyst encapsulating [NiFe]-hydrogenases for enhanced hydrogen production

Hydrogenases are microbial metalloenzymes capable of catalyzing the reversible interconversion between molecular hydrogen and protons with high efficiency, and have great potential in the development of new electrocatalysts for renewable...</jats:p

N-doped carbon shell encapsulated PtZn intermetallic nanoparticles as highly efficient catalysts for fuel cells

Abstract(#br)The high cost and poor durability of Pt nanoparticles (NPs) have always been great challenges to the commercialization of proton exchange membrane fuel cells (PEMFCs). Pt-based intermetallic NPs with a highly ordered structure are considered as promising catalysts for PEMFCs due to their high catalytic activity and stability. Here, we reported a facile method to synthesize N-doped carbon encapsulated PtZn intermetallic (PtZn@NC) NPs via the pyrolysis of Pt@Zn-based zeolitic imidazolate framework-8 (Pt@ZIF-8) composites. The catalyst obtained at 800 °C (10%-PtZn@NC-800) was found to exhibit a half-wave potential ( E 1/2 ) up to 0.912 V versus reversible hydrogen electrode (RHE) for the cathodic oxygen reduction reaction in an acidic medium, which shifted by 26 mV positively..

Protein kinase A negatively regulates Ca2+ signalling in Toxoplasma gondii.

The phylum Apicomplexa comprises a group of obligate intracellular parasites that alternate between intracellular replicating stages and actively motile extracellular forms that move through tissue. Parasite cytosolic Ca2+ signalling activates motility, but how this is switched off after invasion is complete to allow for replication to begin is not understood. Here, we show that the cyclic adenosine monophosphate (cAMP)-dependent protein kinase A catalytic subunit 1 (PKAc1) of Toxoplasma is responsible for suppression of Ca2+ signalling upon host cell invasion. We demonstrate that PKAc1 is sequestered to the parasite periphery by dual acylation of PKA regulatory subunit 1 (PKAr1). Upon genetic depletion of PKAc1 we show that newly invaded parasites exit host cells shortly thereafter, in a perforin-like protein 1 (PLP-1)-dependent fashion. Furthermore, we demonstrate that loss of PKAc1 prevents rapid down-regulation of cytosolic [Ca2+] levels shortly after invasion. We also provide evidence that loss of PKAc1 sensitises parasites to cyclic GMP (cGMP)-induced Ca2+ signalling, thus demonstrating a functional link between cAMP and these other signalling modalities. Together, this work provides a new paradigm in understanding how Toxoplasma and related apicomplexan parasites regulate infectivity

Advances in nanocatalysts design for biofuels production

The exploitation of nanocatalysts, at the boundary between homogeneous and heterogeneous catalysis, is tracking new efficient ways to produce renewable biofuels in environmentally friendly conditions. Their solid state makes them recyclable, and their nanomateric particle size enables high activities approaching those offered by homogeneous catalysts, as well as novel and unique catalytic behaviors not accessible to solids above the nanometer range. Furthermore, the use of magnetically active materials has led to the development of nanocatalysts easily recoverable through the application of magnetic fields. In this mini-review, latest achievements in the production of advanced biofuels using stable, highly active, cheap and reusable nanocatalysts are described. Specifically, biodiesel and high density fuels have been chosen as major topics of research for the design of catalytic nanomaterials