Study on the preparation and properties of UV curable polyurethane materials modified by organic silicon

In this paper, polyester polyol, toluene diisocyanate (TDI) and hydroxyethyl acrylate (HEA) were taken as the raw materials to synthesize polyurethane acrylate. UV curable organic silicon nano sol was synthesized by γ-methacryloxypropyltrime-thoxysilane. The UV curable polyurethane materials modified by organic silicon were prepared based on the UV curable organic silicon nano sol and synthesized polyurethane acrylate. This paper studies on the mechanical properties of organic silicon modified UV curable polyurethane materials and puts forward the micro model that inorganic phase is bonded to the resin in the form of chemical bonds. The results show that the heat resistance and mechanical properties of hybrid materials are improved with the increase of the nano silica sol’s contents , the abrasion resistance of hybrid coatings is also improved and the silica nano particles are well dispersed in the organic phase

Study on the preparation and properties of UV curable polyurethane materials modified by organic silicon

In this paper, polyester polyol, toluene diisocyanate (TDI) and hydroxyethyl acrylate (HEA) were taken as the raw materials to synthesize polyurethane acrylate. UV curable organic silicon nano sol was synthesized by γ-methacryloxypropyltrime-thoxysilane. The UV curable polyurethane materials modified by organic silicon were prepared based on the UV curable organic silicon nano sol and synthesized polyurethane acrylate. This paper studies on the mechanical properties of organic silicon modified UV curable polyurethane materials and puts forward the micro model that inorganic phase is bonded to the resin in the form of chemical bonds. The results show that the heat resistance and mechanical properties of hybrid materials are improved with the increase of the nano silica sol’s contents , the abrasion resistance of hybrid coatings is also improved and the silica nano particles are well dispersed in the organic phase

Identification of Candidate Genes Associated with Charcot-Marie-Tooth Disease by Network and Pathway Analysis

Charcot-Marie-Tooth Disease (CMT) is the most common clinical genetic disease of the peripheral nervous system. Although many studies have focused on elucidating the pathogenesis of CMT, few focuses on achieving a systematic analysis of biology to decode the underlying pathological molecular mechanisms and the mechanism of its disease remains to be elucidated. So our study may provide further useful insights into the molecular mechanisms of CMT based on a systematic bioinformatics analysis. In the current study, by reviewing the literatures deposited in PUBMED, we identified 100 genes genetically related to CMT. Then, the functional features of the CMT-related genes were examined by R software and KOBAS, and the selected biological process crosstalk was visualized with the software Cytoscape. Moreover, CMT specific molecular network analysis was conducted by the Molecular Complex Detection (MCODE) Algorithm. The biological function enrichment analysis suggested that myelin sheath, axon, peripheral nervous system, mitochondrial function, various metabolic processes, and autophagy played important roles in CMT development. Aminoacyl-tRNA biosynthesis, metabolic pathways, and vasopressin-regulated water reabsorption were significantly enriched in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway network, suggesting that these pathways may play key roles in CMT occurrence and development. According to the crosstalk, the biological processes could be roughly divided into a correlative module and two separate modules. MCODE clusters showed that in top 3 clusters, 13 of CMT-related genes were included in the network and 30 candidate genes were discovered which might be potentially related to CMT. The study may help to update the new understanding of the pathogenesis of CMT and expand the potential genes of CMT for further exploration

Research on the Hydrophilic Modified of LDPE for the New Biological Suspended Filler

Urban sewage is one of the main pollution sources of the city, which pollute soil, deteriorate the water quality and increase the water shortages and urban load. LDPE is low cost and widely used as the basic material of wastewater treatment, but LDPE’s hydrophilic is not good enough to meet the need of suspended filler in wastewater treatment. In this paper the hydrophilic modified of LDPE for the new biological suspended filler was studied and the preparation and processing technique based on LDPE was researched. The hydrophilic and mechanic performance of the hydrophilic modified materials was tested. Results shown that the new type of hydrophilic modified materials has good hydrophilic and meets the demand of urban sewage treatment. The research on the new suspended filler materials has great meaning in solving the problem of urban sewage and recycling

Ligation-Dependent Cas14a1-Activated Biosensor for One-pot Pathogenic Diagnostic

Pathogenic identification requires nucleic acid diagnosis with simple equipment and fast manipulation. Our work established an all-in-one strategy assay with excellent sensitivity and high specificity, Transcription-Amplified Cas14a1-Activated Signal Biosensor (TACAS), for the fluorescence-based bacterial RNA detection. The DNA as a promoter probe and a reporter probe directly ligated via SplintR ligase once specifically hybridized to the single-stranded target RNA sequence, with the ligation product transcribed into Cas14a1 RNA activators by T7 RNA polymerase. This forming sustained isothermal one-pot ligation-transcription cascade produced RNA activators constantly and enabled Cas14a1/sgRNA complex to generate fluorescence signal, thus leading to a sensitive detection limit of 1 CFU/mL E.coli within 2-3 h of incubation time. TACAS was applied in contrived E.coli infected fish samples, and a significant signal differentiation between positive (infected) and negative (uninfected) samples was reached. Meanwhile, E.coli colonization and transmit time in vivo were explored and the TACAS assay promoted the understanding of the infection mechanisms of the E.coli infection, demonstrating an excellent detection capability

A Human Brain Model Mimicking Umbilical Cord Mesenchymal Stem Cells for the Treatment of Hypoxic-Ischemic Brain Injury

We used an in vitro model of the human brain immune microenvironment to simulate hypoxic-ischemic brain injury (HIBI) and treatment with human umbilical cord mesenchymal stem cells (hUMSCs) to address the transformation barriers of gene differences between animals and humans in preclinical research. A co-culture system, termed hNAME, consisted of human hippocampal neurons (N), astrocytes (A), microglia (M), and brain microvascular endothelial cells (E). Flow cytometry measured the apoptosis rates of neurons and endothelial cells. hNAME-neurons and endothelial cells experienced more severe damage than monolayer cells, particularly after 48 h and 24 h of reoxygenation (OGD48/R24). Western blotting identified neuroinflammatory response markers, including HIF-1α, C1q, C3, TNF-α, and iNOS. Inflammatory factors originated from the glial chamber rather than the neurons and vascular endothelial chambers. A gradual increase in the release of inflammatory factors was observed as the OGD and reoxygenation times increased, peaking at OGD48/R24. The hNAME value was confirmed in human umbilical cord mesenchymal stem cells (hUMSCs). Treatment with hUMSCs resulted in a notable decrease in the severity of neuronal and endothelial cell damage in hNAME. The hNAME is an ideal in vitro model for simulating the immune microenvironment of the human brain because of the interactions between neurons, vessels, astrocytes, and microglia

Formation of autotriploid Carassius auratus and its fertility-related genes analysis

Abstract Background Formation of triploid organism is useful in genetics and breeding. In this study, autotriploid Carassius auratus (3nRR, 3n = 150) was generated from Carassius auratus red var. (RCC, 2n = 100) (♀) and autotetraploid Carassius auratus (4nRR, 4n = 200) (♂). The female 3nRR produced haploid, diploid and triploid eggs, whereas the male 3nRR was infertile. The aim of the present study was to explore fertility of potential candidate genes of 3nRR. Results Gonadal transcriptome profiling of four groups (3 females RCC (FRCC), 3 males 4nRR (M4nRR), 3 males 3nRR (M3nRR) and 3 females 3nRR (F3nRR)) was performed using RNA-SEq. A total of 78.90 Gb of clean short reads and 24,262 differentially expressed transcripts (DETs), including 20,155 in F3nRR vs. FRCC and 4,107 in M3nRR vs. M4nRR were identified. A total of 106 enriched pathways were identified through KEGG enrichment analysis. Out of the enriched pathways, 44 and 62 signalling pathways were identified in F3nRR vs. FRCC and M3nRR vs. M4nRR, respectively. A total of 80 and 25 potential candidate genes for fertility-related in F3nRR and M3nRR were identified, respectively, through GO, KEGG analyses and the published literature. Moreover, protein-protein interaction (PPI) network construction of these fertility-associated genes were performed. Analysis of the PPI networks showed that 6 hub genes (MYC, SOX2, BMP4, GATA4, PTEN and BMP2) were involved in female fertility of F3nRR, and 2 hub genes (TP53 and FGF2) were involved in male sterility of M3nRR. Conclusions Establishment of autotriploid fish offers an ideal model to study reproductive traits of triploid fish. RNA-Seq data revealed 6 genes, namely, MYC, SOX2, BMP4, GATA4, PTEN and BMP2, involved in the female fertility of the F3nRR. Moreover, 2 genes, namely, TP53 and FGF2, were related to the male sterility of the M3nRR. These findings provide information on reproduction and breeding in triploid fish