21 research outputs found
Insecticidal Activity of \u3ci\u3eBacillus thuringiensis\u3c/i\u3e Cry1Bh1 against \u3ci\u3eOstrinia nubilalis\u3c/i\u3e (Hübner) (Lepidoptera: Crambidae) and Other Lepidopteran Pests
Bacillus thuringiensis is an important source of insect resistance traits in commercial crops. In an effort to prolong B. thuringiensis trait durability, insect resistance management programs often include combinations of insecticidal proteins that are not cross resistant or have demonstrable differences in their site of action as a means to mitigate the development of resistant insect populations. In this report, we describe the activity spectrum of a novel B. thuringiensis Cry protein, Cry1Bh1, against several lepidopteran pests, including laboratory-selected B. thuringiensis-resistant strains of Ostrinia nubilalis and Heliothis virescens and progeny of field-evolved B. thuringiensis-resistant strains of Plutella xylostella and Spodoptera frugiperda. Cry1Bh1 is active against susceptible and B. thuringiensis-resistant colonies of O. nubilalis, P. xylostella, and H. virescens in laboratory diet-based assays, implying a lack of cross-resistance in these insects. However, Cry1Bh1 is not active against susceptible or Cry1F-resistant S. frugiperda. Further, Cry1Bh1 does not compete with Cry1Fa or Cry1Ab for O. nubilalis midgut brush border membrane binding sites. Cry1Bh1-expressing corn, while not completely resistant to insect damage, provided significantly better leaf protection against Cry1Fa-resistant O. nubilalis than did Cry1Fa-expressing hybrid corn. The lack of cross-resistance with Cry1Ab and Cry1Fa along with independent membrane binding sites in O. nubilalis makes Cry1Bh1 a candidate to further optimize for in-plant resistance to this pest
Resolving Globular Cluster Formation within a Cosmological Context
We place constraints on the formation redshifts for blue globular clusters
(BGCs), independent of the details of hydrodynamics and population III star
formation. The observed radial distribution of BGCs in the Milky Way Galaxy
suggests that they formed in biased dark matter halos at high redshift. As a
result, simulations of a ~1 Mpc box up to z~10 must resolve BGC formation in
LCDM. We find that most halo stars could be produced from destroyed BGCs and
other low-mass clusters that formed at high redshift. We present a
proof-of-concept simulation that captures the formation of globular-like star
clusters.Comment: Accepted for publication in ApJ
Evaluating Systematic Dependencies of Type Ia Supernovae: The Influence of Deflagration to Detonation Density
We explore the effects of the deflagration to detonation transition (DDT)
density on the production of Ni-56 in thermonuclear supernova explosions (type
Ia supernovae). Within the DDT paradigm, the transition density sets the amount
of expansion during the deflagration phase of the explosion and therefore the
amount of nuclear statistical equilibrium (NSE) material produced. We employ a
theoretical framework for a well-controlled statistical study of
two-dimensional simulations of thermonuclear supernovae with randomized initial
conditions that can, with a particular choice of transition density, produce a
similar average and range of Ni-56 masses to those inferred from observations.
Within this framework, we utilize a more realistic "simmered" white dwarf
progenitor model with a flame model and energetics scheme to calculate the
amount of Ni-56 and NSE material synthesized for a suite of simulated
explosions in which the transition density is varied in the range 1-3x10^7
g/cc. We find a quadratic dependence of the NSE yield on the log of the
transition density, which is determined by the competition between plume rise
and stellar expansion. By considering the effect of metallicity on the
transition density, we find the NSE yield decreases by 0.055 +/- 0.004 solar
masses for a 1 solar metallicity increase evaluated about solar metallicity.
For the same change in metallicity, this result translates to a 0.067 +/- 0.004
solar mass decrease in the Ni-56 yield, slightly stronger than that due to the
variation in electron fraction from the initial composition. Observations
testing the dependence of the yield on metallicity remain somewhat ambiguous,
but the dependence we find is comparable to that inferred from some studies.Comment: 15 pages, 13 figures, accepted to ApJ on July 6, 201
Modules for Experiments in Stellar Astrophysics (MESA)
Stellar physics and evolution calculations enable a broad range of research
in astrophysics. Modules for Experiments in Stellar Astrophysics (MESA) is a
suite of open source libraries for a wide range of applications in
computational stellar astrophysics. A newly designed 1-D stellar evolution
module, MESA star, combines many of the numerical and physics modules for
simulations of a wide range of stellar evolution scenarios ranging from
very-low mass to massive stars, including advanced evolutionary phases. MESA
star solves the fully coupled structure and composition equations
simultaneously. It uses adaptive mesh refinement and sophisticated timestep
controls, and supports shared memory parallelism based on OpenMP. Independently
usable modules provide equation of state, opacity, nuclear reaction rates, and
atmosphere boundary conditions. Each module is constructed as a separate
Fortran 95 library with its own public interface. Examples include comparisons
to other codes and show evolutionary tracks of very low mass stars, brown
dwarfs, and gas giant planets; the complete evolution of a 1 Msun star from the
pre-main sequence to a cooling white dwarf; the Solar sound speed profile; the
evolution of intermediate mass stars through the thermal pulses on the He-shell
burning AGB phase; the interior structure of slowly pulsating B Stars and Beta
Cepheids; evolutionary tracks of massive stars from the pre-main sequence to
the onset of core collapse; stars undergoing Roche lobe overflow; and accretion
onto a neutron star. Instructions for downloading and installing MESA can be
found on the project web site (http://mesa.sourceforge.net/).Comment: 110 pages, 39 figures; submitted to ApJS; visit the MESA website at
http://mesa.sourceforge.ne
SN 2009bb: a Peculiar Broad-Lined Type Ic Supernova
Ultraviolet, optical, and near-infrared photometry and optical spectroscopy
of the broad-lined Type Ic supernova (SN) 2009bb are presented, following the
flux evolution from -10 to +285 days past B-band maximum. Thanks to the very
early discovery, it is possible to place tight constraints on the SN explosion
epoch. The expansion velocities measured from near maximum spectra are found to
be only slightly smaller than those measured from spectra of the prototype
broad-lined SN 1998bw associated with GRB 980425. Fitting an analytical model
to the pseudo-bolometric light curve of SN 2009bb suggests that 4.1+-1.9 Msun
of material was ejected with 0.22 +-0.06 Msun of it being 56Ni. The resulting
kinetic energy is 1.8+-0.7x10^52 erg. This, together with an absolute peak
magnitude of MB=-18.36+-0.44, places SN 2009bb on the energetic and luminous
end of the broad-lined Type Ic (SN Ic) sequence. Detection of helium in the
early time optical spectra accompanied with strong radio emission, and high
metallicity of its environment makes SN 2009bb a peculiar object. Similar to
the case for GRBs, we find that the bulk explosion parameters of SN 2009bb
cannot account for the copious energy coupled to relativistic ejecta, and
conclude that another energy reservoir (a central engine) is required to power
the radio emission. Nevertheless, the analysis of the SN 2009bb nebular
spectrum suggests that the failed GRB detection is not imputable to a large
angle between the line-of-sight and the GRB beamed radiation. Therefore, if a
GRB was produced during the SN 2009bb explosion, it was below the threshold of
the current generation of gamma-ray instruments.Comment: Accepted for publication in Ap
Catching Element Formation In The Act
Gamma-ray astronomy explores the most energetic photons in nature to address
some of the most pressing puzzles in contemporary astrophysics. It encompasses
a wide range of objects and phenomena: stars, supernovae, novae, neutron stars,
stellar-mass black holes, nucleosynthesis, the interstellar medium, cosmic rays
and relativistic-particle acceleration, and the evolution of galaxies. MeV
gamma-rays provide a unique probe of nuclear processes in astronomy, directly
measuring radioactive decay, nuclear de-excitation, and positron annihilation.
The substantial information carried by gamma-ray photons allows us to see
deeper into these objects, the bulk of the power is often emitted at gamma-ray
energies, and radioactivity provides a natural physical clock that adds unique
information. New science will be driven by time-domain population studies at
gamma-ray energies. This science is enabled by next-generation gamma-ray
instruments with one to two orders of magnitude better sensitivity, larger sky
coverage, and faster cadence than all previous gamma-ray instruments. This
transformative capability permits: (a) the accurate identification of the
gamma-ray emitting objects and correlations with observations taken at other
wavelengths and with other messengers; (b) construction of new gamma-ray maps
of the Milky Way and other nearby galaxies where extended regions are
distinguished from point sources; and (c) considerable serendipitous science of
scarce events -- nearby neutron star mergers, for example. Advances in
technology push the performance of new gamma-ray instruments to address a wide
set of astrophysical questions.Comment: 14 pages including 3 figure
Insecticidal Activity of Bacillus thuringiensis Cry1Bh1 against Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae) and Other Lepidopteran Pests
Cooperative adaptation to therapy (CAT) confers resistance in heterogeneous non-small cell lung cancer.
Understanding intrinsic and acquired resistance is crucial to overcoming cancer chemotherapy failure. While it is well-established that intratumor, subclonal genetic and phenotypic heterogeneity significantly contribute to resistance, it is not fully understood how tumor sub-clones interact with each other to withstand therapy pressure. Here, we report a previously unrecognized behavior in heterogeneous tumors: cooperative adaptation to therapy (CAT), in which cancer cells induce co-resistant phenotypes in neighboring cancer cells when exposed to cancer therapy. Using a CRISPR/Cas9 toolkit we engineered phenotypically diverse non-small cell lung cancer (NSCLC) cells by conferring mutations in Dicer1, a type III cytoplasmic endoribonuclease involved in small non-coding RNA genesis. We monitored three-dimensional growth dynamics of fluorescently-labeled mutant and/or wild-type cells individually or in co-culture using a substrate-free NanoCulture system under unstimulated or drug pressure conditions. By integrating mathematical modeling with flow cytometry, we characterized the growth patterns of mono- and co-cultures using a mathematical model of intra- and interspecies competition. Leveraging the flow cytometry data, we estimated the model's parameters to reveal that the combination of WT and mutants in co-cultures allowed for beneficial growth in previously drug sensitive cells despite drug pressure via induction of cell state transitions described by a cooperative game theoretic change in the fitness values. Finally, we used an ex vivo human tumor model that predicts clinical response through drug sensitivity analyses and determined that cellular and morphologic heterogeneity correlates to prognostic failure of multiple clinically-approved and off-label drugs in individual NSCLC patient samples. Together, these findings present a new paradox in drug resistance implicating non-genetic cooperation among tumor cells to thwart drug pressure, suggesting that profiling for druggable targets (i.e. mutations) alone may be insufficient to assign effective therapy