The Space Shuttle Main Engine And Its Maintenance Features

The Space Shuttle Main Engine* (SSME) is a reusable, high-performance rocket engine being developed to satisfy the performance, life, reliability, and operational requirements of the Space Shuttle Qrbi- ter. The design includes simple, low-cost maintenance features resulting from a viable maintainability program dedicated to minimizing engine cost per flight

Printable Flexible Robots for Remote Learning

The COVID-19 pandemic has revealed the importance of digital fabrication to enable online learning, which remains a challenge for robotics courses. We introduce a teaching methodology that allows students to participate remotely in a hands-on robotics course involving the design and fabrication of robots. Our methodology employs 3D printing techniques with flexible filaments to create innovative soft robots; robots are made from flexible, as opposed to rigid, materials. Students design flexible robotic components such as actuators, sensors, and controllers using CAD software, upload their designs to a remote 3D printing station, monitor the print with a web camera, and inspect the components with lab staff before being mailed for testing and assembly. At the end of the course, students will have iterated through several designs and created fluidically-driven soft robots. Our remote teaching methodology enables educators to utilize 3D printing resources to teach soft robotics and cultivate creativity among students to design novel and innovative robots. Our methodology seeks to democratize robotics engineering by decoupling hands-on learning experiences from expensive equipment in the learning environment.Comment: 9 pages, 4 figures, peer reviewed and presented paper at American Society of Engineering Education, April 22-23rd, 2022 - Wentworth Institute of Technolog

Millimeter observations and modeling of the AB Aurigae system

(Abriged) We present the results of millimeter observations and a suitable chemical and radiative transfer model of the AB Aur (HD 31293) circumstellar disk and surrounding envelope. The integral molecular content of this system is studied by observing CO, C$^{18}$O, CS, HCO$^+$, DCO$^+$, H$_2$CO, HCN, HNC, and SiO rotational lines with the IRAM 30-m antenna, while the disk is mapped in the HCO$^+$(1-0) transition with the Plateau de Bure interferometer. Using a flared disk model with a vertical temperature gradient and an isothermal spherical envelope model with a shadowed midplane and two unshielded cones together with a gas-grain chemical network, time-dependent abundances of observationally important molecules are calculated. Then a 2D non-LTE line radiative transfer code is applied to compute excitation temperatures of several rotational transitions of HCO$^+$, CO, C$^{18}$O, and CS molecules. We synthesize the HCO$^+$(1-0) interferometric map along with single-dish CO(2-1), C$^{18}$O(2-1), HCO$^+$(1-0), HCO$^+$(3-2), CS(2-1), and CS(5-4) spectra and compared them with the observations. Our disk model successfully reproduces observed interferometric HCO$^+$(1-0) data, thereby constraining the following disk properties: (1) the inclination angle \iota=17^{+6}_{-3}\degr, (2) the position angle \phi=80\pm30\degr, (3) the size $R_\mathrm{out}=400\pm200$ AU, (4) the mass M_\mathrm{disk}=1.3\cdot10^{-2} M_{\sun} (with a factor of $\sim7$ uncertainty), and (5) that the disk is in Keplerian rotation. Furthermore, indirect evidence for a local inhomogeneity of the envelope at \ga600 AU is found...Comment: 62 pages, 13 figures, 7 tables, accepted for publication in Ap

Important steps towards a big change for lung health: a joint approach by the European Respiratory Society, the European Society of Radiology and their partners to facilitate implementation of the European Union's new recommendations on lung cancer screening.

Enormous progress has been made on the epic journey towards implementation of lung cancer screening in Europe. A breakthrough for lung health has been achieved with the EU proposal for a Council recommendation on cancer screening. https://bit.ly/3J4O0Jb

Extragalactic millimeter-wave sources in South Pole Telescope survey data: source counts, catalog, and statistics for an 87 square-degree field

We report the results of an 87 square-degree point-source survey centered at R.A. 5h30m, decl. -55 deg. taken with the South Pole Telescope (SPT) at 1.4 and 2.0 mm wavelengths with arc-minute resolution and milli-Jansky depth. Based on the ratio of flux in the two bands, we separate the detected sources into two populations, one consistent with synchrotron emission from active galactic nuclei (AGN) and one consistent with thermal emission from dust. We present source counts for each population from 11 to 640 mJy at 1.4 mm and from 4.4 to 800 mJy at 2.0 mm. The 2.0 mm counts are dominated by synchrotron-dominated sources across our reported flux range; the 1.4 mm counts are dominated by synchroton-dominated sources above ~15 mJy and by dust-dominated sources below that flux level. We detect 141 synchrotron-dominated sources and 47 dust-dominated sources at S/N > 4.5 in at least one band. All of the most significantly detected members of the synchrotron-dominated population are associated with sources in previously published radio catalogs. Some of the dust-dominated sources are associated with nearby (z << 1) galaxies whose dust emission is also detected by the Infrared Astronomy Satellite (IRAS). However, most of the bright, dust-dominated sources have no counterparts in any existing catalogs. We argue that these sources represent the rarest and brightest members of the population commonly referred to as sub-millimeter galaxies (SMGs). Because these sources are selected at longer wavelengths than in typical SMG surveys, they are expected to have a higher mean redshift distribution and may provide a new window on galaxy formation in the early universe.Comment: 35 emulateapj pages, 12 figures, 5 table

P120-Catenin Isoforms 1 and 3 Regulate Proliferation and Cell Cycle of Lung Cancer Cells via β-Catenin and Kaiso Respectively

<div><h3>Background</h3><p>The different mechanisms involved in p120-catenin (p120ctn) isoforms' 1/3 regulation of cell cycle progression are still not elucidated to date.</p> <h3>Methods and Findings</h3><p>We found that both cyclin D1 and cyclin E could be effectively restored by restitution of p120ctn-1A or p120ctn-3A in p120ctn depleted lung cancer cells. When the expression of cyclin D1 was blocked by co-transfection with siRNA-cyclin D1 in p120ctn depleted cells restoring p120ctn-1A or 3A, the expression of cyclin E was slightly decreased, not increased, implying that p120ctn isoforms 1 and 3 cannot up-regulate cyclin E directly but may do so through up-regulation of cyclin D1. Interestingly, overexpression of p120ctn-1A increased β-catenin and cyclin D1 expression, while co-transfection with siRNA targeting β-catenin abolishes the effect of p120ctn-1A on up-regulation of cyclin D1, suggesting a role of β-catenin in mediating p120ctn-1A's regulatory function on cyclin D1 expression. On the other hand, overexpression of p120ctn isoform 3A reduced nuclear Kaiso localization, thus decreasing the binding of Kaiso to KBS on the cyclin D1 promoter and thereby enhancing the expression of cyclin D1 gene by relieving the repressor effect of Kaiso. Because overexpressing NLS-p120ctn-3A (p120ctn-3A nuclear target localization plasmids) or inhibiting nuclear export of p120ctn-3 by Leptomycin B (LMB) caused translocation of Kaiso to the nucleus, it is plausible that the nuclear export of Kaiso is p120ctn-3-dependent.</p> <h3>Conclusions</h3><p>Our results suggest that p120ctn isoforms 1 and 3 up-regulate cyclin D1, and thereby cyclin E, resulting in the promotion of cell proliferation and cell cycle progression in lung cancer cells probably via different protein mediators, namely, β-catenin for isoform 1 and Kaiso, a negative transcriptional factor of cyclin D1, for isoform 3.</p> </div

Epithelial to Mesenchymal Transition by TGFβ-1 Induction Increases Stemness Characteristics in Primary Non Small Cell Lung Cancer Cell Line

Cancer Stem Cells (CSCs) hypothesis asserts that only a small subset of cells within a tumour is capable of both tumour initiation and sustainment. The Epithelial-Mesenchymal Transition (EMT) is an embryonic developmental program that is often activated during cancer invasion and metastasis. The aim of this study is to shed light on the relationship between EMT and CSCs by using LC31 lung cancer primary cell line.A549 and LC31 cell lines were treated with 2 ng/ml TGFβ-1 for 30 days, and 80 days, respectively. To evaluate EMT, morphological changes were assessed by light microscopy, immunofluorescence and cytometry for following markers: cytokeratins, e-cadherin, CD326 (epithelial markers) and CD90, and vimentin (mesenchymal markers). Moreover, RT-PCR for Slug, Twist and β-catenin genes were performed. On TGFβ-1 treated and untreated LC31 cell lines, we performed stemness tests such as pneumospheres growth and stem markers expression such as Oct4, Nanog, Sox2, c-kit and CD133. Western Blot for CD133 and tumorigenicity assays using NOD/SCID mice were performed.TGFβ-1 treated LC31 cell line lost its epithelial morphology assuming a fibroblast-like appearance. The same results were obtained for the A549 cell line (as control). Immunofluorescence and cytometry showed up-regulation of vimentin and CD90 and down-regulation of cytocheratin, e-cadherin and CD326 in TGFβ-1 treated LC31 and A549 cell lines. Slug, Twist and β-catenin m-RNA transcripts were up-regulated in TGFβ-1 treated LC31 cell line confirming EMT. This cell line showed also over-expression of Oct4, Nanog, Sox2 and CD133, all genes of stemness. In addition, in TGFβ-1 treated LC31 cell line, an increased pneumosphere-forming capacity and tumours-forming ability in NOD/SCID mice were detectable.The induction of EMT by TGFβ-1 exposure, in primary lung cancer cell line results in the acquisition of mesenchymal profile and in the expression of stem cell markers

Residual hepatocellular carcinoma after oxaliplatin treatment has increased metastatic potential in a nude mouse model and is attenuated by Songyou Yin

<p>Abstract</p> <p>Background</p> <p>The opposite effects of chemotherapy, which enhance the malignancy of treated cancers such as hepatocellular carcinoma (HCC), are not well understood. We investigated this phenomenon and corresponding mechanisms to develop a novel approach for improving chemotherapy efficacy in HCC.</p> <p>Methods</p> <p>Human hepatocellular carcinoma cell lines HepG2 (with low metastatic potential) and MHCC97L (with moderate metastatic potential) were used for the in vitro study. An orthotopic nude mouse model of human HCC was developed using MHCC97L cells. We then assessed the metastatic potential of surviving tumor cells after in vitro and in vivo oxaliplatin treatment. The molecular changes in surviving tumor cells were evaluated by western blot, immunofluorescence, and immunohistochemistry. The Chinese herbal extract Songyou Yin (composed of five herbs) was investigated in vivo to explore its effect on the metastatic potential of oxaliplatin-treated cancer cells.</p> <p>Results</p> <p>MHCC97L and HepG2 cells surviving oxaliplatin treatment showed enhanced migration and invasion in vitro. Residual HCC after in vivo oxaliplatin treatment demonstrated significantly increased metastasis to the lung (10/12 vs. 3/12) when re-inoculated into the livers of new recipient nude mice. Molecular changes consistent with epithelial-mesenchymal transition (EMT) were observed in oxaliplatin-treated tumor tissues and verified by in vitro experiments. The Chinese herbal extract Songyou Yin (4.2 and 8.4 g/kg) attenuated EMT and inhibited the enhanced metastatic potential of residual HCC in nude mice (6/15 vs. 13/15 and 3/15 vs. 13/15, respectively).</p> <p>Conclusions</p> <p>The surviving HCC after oxaliplatin treatment underwent EMT and demonstrated increased metastatic potential. Attenuation of EMT by Songyou Yin may improve the efficacy of chemotherapy in HCC.</p