Gain control from beyond the classical receptive field in primate primary visual cortex

Gain control is a salient feature of information processing throughout the visual system. Heeger (1991, 1992) described a mechanism that could underpin gain control in primary visual cortex (VI). According to this model, a neuron's response is normalized by dividing its output by the sum of a population of neurons, which are selective for orientations covering a broad range. Gain control in this scheme is manifested as a change in the semisaturation constant (contrast gain) of a VI neuron. Here we examine how flanking and annular gratings of the same or orthogonal orientation to that preferred by a neuron presented beyond the receptive field modulate gain in V1 neurons in anesthetized marmosets (Callithrix jacchus). To characterize how gain was modulated by surround stimuli, the Michaelis-Menten equation was fitted to response versus contrast functions obtained under each stimulus condition. The modulation of gain by surround stimuli was modelled best as a divisive reduction in response gain. Response gain varied with the orientation of surround stimuli, but was reduced most when the orientation of a large annular grating beyond the classical receptive field matched the preferred orientation of neurons. The strength of surround suppression did not vary significantly with retinal eccentricity or laminar distribution. In the mannoset, as in macaques (Angelucci et al., 2002a,b), gain control over the sort of distances reported here (up to 10 deg) may be mediated by feedback from extrastriate areas

X-ray sources and their optical counterparts in the globular cluster M 22

Using XMM-Newton EPIC imaging data, we have detected 50 low-luminosity X-ray sources in the field of view of M 22, where 5 +/- 3 of these sources are likely to be related to the cluster. Using differential optical photometry, we have identified probable counterparts to those sources belonging to the cluster. Using X-ray spectroscopic and timing studies, supported by the optical colours, we propose that the most central X-ray sources in the cluster are cataclysmic variables, millisecond pulsars, active binaries and a blue straggler. We also identify a cluster of galaxies behind this globular cluster.Comment: 11 pages, 7 figures, accepted for publication in A&

Optical variability of the accretion disk around the intermediate mass black hole ESO 243-49 HLX-1 during the 2012 outburst

We present dedicated quasi-simultaneous X-ray (Swift) and optical (Very Large Telescope (VLT), V- and R-band) observations of the intermediate mass black hole candidate ESO 243-49 HLX-1 before and during the 2012 outburst. We show that the V-band magnitudes vary with time, thus proving that a portion of the observed emission originates in the accretion disk. Using the first quiescent optical observations of HLX-1, we show that the stellar population surrounding HLX-1 is fainter than V~25.1 and R~24.2. We show that the optical emission may increase before the X-ray emission consistent with the scenario proposed by Lasota et al. (2011) in which the regular outbursts could be related to the passage at periastron of a star circling the intermediate mass black hole in an eccentric orbit, which triggers mass transfer into a quasi-permanent accretion disk around the black hole. Further, if there is indeed a delay in the X-ray emission we estimate the mass-transfer delivery radius to be ~1e11 cm.Comment: 9 pages, 2 figures, accepted for publication in ApJ

Cephalosporin-3’-diazeniumdiolate NO-donor prodrug PYRRO-C3D enhances azithromycin susceptibility of non-typeable Haemophilus influenzae biofilms

The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link.Objectives: PYRRO-C3D is a cephalosporin-3-diazeniumdiolate nitric oxide (NO)-donor prodrug designed to selectively deliver NO to bacterial infection sites. The objective of this study was to assess the activity of PYRRO-C3D against non-typeable Haemophilus influenzae (NTHi) biofilms and examine the role of NO in reducing biofilm-associated antibiotic tolerance. Methods: The activity of PYRRO-C3D on in vitro NTHi biofilms was assessed through CFU enumeration and confocal microscopy. NO release measurements were performed using an ISO-NO probe. NTHi biofilms grown on primary ciliated respiratory epithelia at an air-liquid interface were used to investigate the effects of PYRRO-C3D in the presence of host tissue. Label-free LC/MS proteomic analyses were performed to identify differentially expressed proteins following NO treatment. Results: PYRRO-C3D specifically released NO in the presence of NTHi, while no evidence of spontaneous NO release was observed when the compound was exposed to primary epithelial cells. NTHi lacking β-lactamase activity failed to trigger NO release. Treatment significantly increased the susceptibility of in vitro NTHi biofilms to azithromycin, causing a log-fold reduction in viability (p<0.05) relative to azithromycin alone. The response was more pronounced for biofilms grown on primary respiratory epithelia, where a 2-log reduction was observed (p<0.01). Label-free proteomics showed that NO increased expression of sixteen proteins involved in metabolic and transcriptional/translational functions. Conclusions: NO release from PYRRO-C3D enhances the efficacy of azithromycin against NTHi biofilms, putatively via modulation of NTHi metabolic activity. Adjunctive therapy with NO mediated through PYRRO-C3D represents a promising approach for reducing biofilm associated antibiotic tolerance

Investigating slim disk solutions for HLX-1 in ESO 243-49

The hyper luminous X-ray source HLX-1 in the galaxy ESO 243-49, currently the best intermediate mass black hole candidate, displays spectral transitions similar to those observed in Galactic black hole binaries, but with a luminosity 100-1000 times higher. We investigated the X-ray properties of this unique source fitting multi-epoch data collected by Swift, XMM-Newton & Chandra with a disk model computing spectra for a wide range of sub- and super-Eddington accretion rates assuming a non-spinning black hole and a face-on disk (i = 0 deg). Under these assumptions we find that the black hole in HLX-1 is in the intermediate mass range (~2 x 10^4 M_odot) and the accretion flow is in the sub-Eddington regime. The disk radiation efficiency is eta = 0.11 +/-0.03. We also show that the source does follow the L_X ~ T^4 relation for our mass estimate. At the outburst peaks, the source radiates near the Eddington limit. The accretion rate then stays constant around 4 x 10^(-4) M_odot yr^(-1) for several days and then decreases exponentially. Such "plateaus" in the accretion rate could be evidence that enhanced mass transfer rate is the driving outburst mechanism in HLX-1. We also report on the new outburst observed in August 2011 by the Swift-X-ray Telescope. The time of this new outburst further strengthens the ~1 year recurrence timescale.Comment: 24 pages, 10 figures, accepted for publication in Ap

Specifying computer-supported collaboration scripts

Collaboration scripts are activity programs which aim to foster collaborative learning by structuring interaction between learners. Computer-supported collaboration scripts generally suffer from the problem of being restrained to a specific learning platform and learning context. A standardization of collaboration scripts first requires a specification of collaboration scripts that integrates multiple perspectives from computer science, education and psychology. So far, only few and limited attempts at such specifications have been made. This paper aims to consolidate and expand these approaches in light of recent findings and to propose a generic framework for the specification of collaboration scripts. The framework enables a description of collaboration scripts using a small number of components (participants, activities, roles, resources and groups) and mechanisms (task distribution, group formation and sequencing)

A far-UV survey of three hot, metal-polluted white dwarf stars: WD0455-282, WD0621-376, and WD2211-495

Using newly obtained high-resolution data ($R\sim{1\times{10}^{5}}$) from the \textit{Hubble Space Telescope}, and archival UV data from the \textit{Far Ultraviolet Spectroscopic Explorer} we have conducted a detailed UV survey of the three hot, metal-polluted white dwarfs WD0455-282, WD0621-376, and WD2211-495. Using bespoke model atmospheres we measured $T_{\mathrm{eff}}$, log $g$, and photospheric abundances for these stars. In conjunction with data from Gaia we measured masses, radii, and gravitational redshift velocities for our sample of objects. We compared the measured photospheric abundances with those predicted by radiative levitation theory, and found that the observed Si abundances in all three white dwarfs, and the observed Fe abundances in WD0621-376 and WD2211-495, were larger than those predicted by an order of magnitude. These findings imply not only an external origin for the metals, but also ongoing accretion, as the metals not supported by radiative levitation would sink on extremely short timescales. We measured the radial velocities of several absorption features along the line of sight to the three objects in our sample, allowing us to determine the velocities of the photospheric and interstellar components along the line of sight for each star. Interestingly, we made detections of circumstellar absorption along the line of sight to WD0455-282 with three velocity components. To our knowledge, this is the first such detection of multi-component circumstellar absorption along the line of sight to a white dwarf.Comment: 19 pages, 23 figures, 8 tables. Accepted for publication in the Monthly Notices of the Royal Astronomical Societ