Byggandet av kundrelationer med hjälp av Internet : en fallstudie om resebyråer

Validerat; 20101217 (root

A study on the development of a web application - Presentshoppen

This report studies the development of a usable web application for gifts with an engaging buying process, where the customer is given suggestions on gifts based on his preferences. The demand was examined and confirmed by studies which formed the basis for the development of the application. The work was done with the project methodology Scrum, which is presented and evaluated. The report presents and discusses the technical solutions chosen for the implementation of the web application.Design choices have been made and are discussed, with the main objective to create ausable web application with an engaging buying process. Usability and commitment hasbeen created through a buying process in which the products are presented one at a time. Finally, the report argues that Presentshoppen is to some extent usable and with an engaging buying process, but it is difficult to prove this before any launch.Denna rapport studerar utvecklandet av en användbar e-butik för presenter med enengagerande köpprocess, där kunden ges förslag på presenter efter dennes preferenser. Behovet av en sådan e-butik kartlades och bekräftades genom studier, vilket låg till grund för utvecklandet av applikationen. Arbetet har genomförts med projektmetodiken Scrum som presenteras och utvärderas. Rapporten presenterar och diskuterar valda tekniska lösningar för implementationen av webbapplikationen. Designmässiga val och avvägningar har genomförts och diskuteras, där huvudsyftet varit att skapa en användbar e-butik med en engagerande köpprocess. Användbarhet och engagemang har huvudsakligen skapats genom en köpprocess där produkter presenteras en i taget. Slutligen argumenterar rapporten för att Presentshoppen till viss del är användbar och engagerande men att svårigheter finns att visa detta innan eventuell lansering

Myosin accumulation and striated muscle myopathy result from the loss of muscle RING finger 1 and 3

Maintenance of skeletal and cardiac muscle structure and function requires precise control of the synthesis, assembly, and turnover of contractile proteins of the sarcomere. Abnormalities in accumulation of sarcomere proteins are responsible for a variety of myopathies. However, the mechanisms that mediate turnover of these long-lived proteins remain poorly defined. We show that muscle RING finger 1 (MuRF1) and MuRF3 act as E3 ubiquitin ligases that cooperate with the E2 ubiquitin–conjugating enzymes UbcH5a, -b, and -c to mediate the degradation of β/slow myosin heavy chain (β/slow MHC) and MHCIIa via the ubiquitin proteasome system (UPS) in vivo and in vitro. Accordingly, mice deficient for MuRF1 and MuRF3 develop a skeletal muscle myopathy and hypertrophic cardiomyopathy characterized by subsarcolemmal MHC accumulation, myofiber fragmentation, and diminished muscle performance. These findings identify MuRF1 and MuRF3 as key E3 ubiquitin ligases for the UPS-dependent turnover of sarcomeric proteins and reveal a potential basis for myosin storage myopathies