A Single-Year Cosmic Ray Event at 5410 BCE Registered in C-14 of Tree Rings

The annual C-14 data in tree rings is an outstanding proxy for uncovering extreme solar energetic particle (SEP) events in the past. Signatures of extreme SEP events have been reported in 774/775 CE, 992/993 CE, and similar to 660 BCE. Here, we report another rapid increase of C-14 concentration in tree rings from California, Switzerland, and Finland around 5410 BCE. These C-14 data series show a significant increase of similar to 6 parts per thousand in 5411-5410 BCE. The signature of C-14 variation is very similar to the confirmed three SEP events and points to an extreme short-term flux of cosmic ray radiation into the atmosphere. The rapid C-14 increase in 5411/5410 BCE rings occurred during a period of high solar activity and 60 years after a grand C-14 excursion during 5481-5471 BCE. The similarity of our C-14 data to previous events suggests that the origin of the 5410 BCE event is an extreme SEP event.Peer reviewe

How should we store avian faecal samples for microbiota analyses? Comparing efficacy and cost-effectiveness

Analyses of bacterial DNA in faecal samples are becoming ever more common, yet we still do not know much about bird microbiomes. These challenges partly lie in the unique chemical nature of their faeces, and in the choice of sample storage method, which affects DNA preservation and the resulting microbiome composition. However, there is little information available on how best to preserve avian faeces for microbial analyses. This study evaluates five widely used methods for preserving nucleic acids and inferring microbiota profiles, for their relative efficacy, cost, and practicality. We tested the five methods (in-situ bead-beating with a TerraLyzer instrument, silica-bead desiccation, ethanol, refrigeration and RNAlater buffer) on 50 fresh faecal samples collected from captive House sparrows (Passer domesticus). In line with other studies, we find that different storage methods lead to distinct bacterial profiles. Storage method had a large effect on community composition and the relative abundance of dominant phyla such as Firmicutes and Proteobacteria, with the most significant changes observed for refrigerated samples. Furthermore, differences in the abundance of aerobic or facultatively aerobic taxa, particularly in refrigerated samples and those stored in ethanol, puts limits on comparisons of bacterial communities across different storage methods. Finally, the methods that did not include in-situ bead-beating did not recover comparable levels of microbiota to the samples that were immediately processed and preserved using a TerraLyzer device. However, this method is also less practical and more expensive under field work circumstances. Our study is the most comprehensive analysis to date on how storage conditions affect subsequent molecular assays applied to avian faeces and provides guidance on cost and practicality of methods under field conditions

Radiocarbon Impact on a Nearby Tree of a Light-Water VVER-Type Nuclear Power Plant, Paks, Hungary

Tree-ring series were collected for radiocarbon analyses from the vicinity of Paks nuclear power plant (NPP)and a background area (Dunaföldvár) for a 10-yr period (2000–2009). Samples of holocellulose were prepared from the woodand converted to graphite for accelerator mass spectrometry (AMS) 14C measurement using the MICADAS at ETH Zürich.The 14C concentration data from these tree rings was compared to the background tree rings for each year. The global decreasingtrend of atmospheric 14C activity concentration was observed in the annual tree rings both in the background area and inthe area of the NPP. As an average of the past 10 yr, the excess 14C emitted by the pressurized-water reactor (PWR) NPP tothe atmosphere shows only a slight systematic excess (~6‰) 14C in the annual rings. The highest 14C excess was 13‰ (in2006); however, years with the same 14C level as the background were quite frequent in the tree-ring series