239 research outputs found
The power of low-resolution spectroscopy: On the spectral classification of planet candidates in the ground-based CoRoT follow-up
Planetary transits detected by the CoRoT mission can be mimicked by a
low-mass star in orbit around a giant star. Spectral classification helps to
identify the giant stars and also early-type stars which are often excluded
from further follow-up.
We study the potential and the limitations of low-resolution spectroscopy to
improve the photometric spectral types of CoRoT candidates. In particular, we
want to study the influence of the signal-to-noise ratio (SNR) of the target
spectrum in a quantitative way. We built an own template library and
investigate whether a template library from the literature is able to reproduce
the classifications. Including previous photometric estimates, we show how the
additional spectroscopic information improves the constraints on spectral type.
Low-resolution spectroscopy (1000) of 42 CoRoT targets covering a
wide range in SNR (1-437) and of 149 templates was obtained in 2012-2013 with
the Nasmyth spectrograph at the Tautenburg 2m telescope. Spectral types have
been derived automatically by comparing with the observed template spectra. The
classification has been repeated with the external CFLIB library.
The spectral class obtained with the external library agrees within a few
sub-classes when the target spectrum has a SNR of about 100 at least. While the
photometric spectral type can deviate by an entire spectral class, the
photometric luminosity classification is as close as a spectroscopic
classification with the external library. A low SNR of the target spectrum
limits the attainable accuracy of classification more strongly than the use of
external templates or photometry. Furthermore we found that low-resolution
reconnaissance spectroscopy ensures that good planet candidates are kept that
would otherwise be discarded based on photometric spectral type alone.Comment: accepted for publication in Astronomische Nachrichten; 12 pages, 4
figures, 7 table
Microbiological evaluation of a new growth-based approach for rapid detection of methicillin-resistant Staphylococcus aureus
OBJECTIVES: Recently, a rapid screening tool for methicillin-resistant Staphylococcus aureus (MRSA) has been introduced that applies a novel detection technology allowing the rapid presence or absence of MRSA to be determined from an enrichment broth after only a few hours of incubation. To evaluate the reliability of this new assay to successfully detect MRSA strains of different origin and clonality, well-characterized S. aureus strains were tested in this study. METHODS: More than 700 methicillin-susceptible and methicillin-resistant strains covering >90% of all registered European MRSA spa types within the SeqNet network were studied. RESULTS: All 513 MRSA strains tested were recognized as methicillin-resistant: among these, 96 MRSA strains were from an institutional collection, each presenting a unique spa type. None of the 211 methicillin-susceptible strains were detected as positive. CONCLUSIONS: The new growth-based rapid MRSA assay was shown to detect without exception all MRSA strains of large collections of strains comprising highly diverse genetic backgrounds, indicating that such a phenotypic test might be potentially more likely to cope with new strains
Effect of Low Temperature on Growth and Ultra-Structure of Staphylococcus spp
The effect of temperature fluctuation is an important factor in bacterial growth especially for pathogens such as the staphylococci that have to remain viable during potentially harsh and prolonged transfer conditions between hosts. The aim of this study was to investigate the response of S. aureus, S. epidermidis, and S. lugdunensis when exposed to low temperature (4°C) for prolonged periods, and how this factor affected their subsequent growth, colony morphology, cellular ultra-structure, and amino acid composition in the non-cytoplasmic hydrolysate fraction. Clinical isolates were grown under optimal conditions and then subjected to 4°C conditions for a period of 8 wks. Cold-stressed and reference control samples were assessed under transmission electron microscopy (TEM) to identify potential ultra-structural changes. To determine changes in amino acid composition, cells were fractured to remove the lipid and cytoplasmic components and the remaining structural components were hydrolysed. Amino acid profiles for the hydrolysis fraction were then analysed for changes by using principal component analysis (PCA). Exposure of the three staphylococci to prolonged low temperature stress resulted in the formation of increasing proportions of small colony variant (SCV) phenotypes. TEM revealed that SCV cells had significantly thicker and more diffuse cell-walls than their corresponding WT samples for both S. aureus and S. epidermidis, but the changes were not significant for S. lugdunensis. Substantial species-specific alterations in the amino acid composition of the structural hydrolysate fraction were also observed in the cold-treated cells. The data indicated that the staphylococci responded over prolonged periods of cold-stress treatment by transforming into SCV populations. The observed ultra-structural and amino acid changes were proposed to represent response mechanisms for staphylococcal survival amidst hostile conditions, thus maintaining the viability of the species until favourable conditions arise again
Spectroscopic properties of cool Ursa Major group members
Until now, most members of the Ursa Major (UMa) group of stars have been
identified by means of kinematic criteria. However, in many cases kinematic
criteria alone are insufficient to ascertain, whether an individual star is
really a member of this group. Since photometric criteria are ineffective in
the case of cool dwarf members, one must use spectroscopic criteria.
Nevertheless, resulting membership criteria are inconclusive. We reanalyse
spectroscopic properties of cool UMa group dwarfs. In particular, we study the
distribution of iron abundance, the strength of the Li I absorption at 6708 A
and the Li abundance, and the infilling of the core of the H alpha line.
Twenty-five cool and northern bona-fide members are carefully selected from the
literature. Homogeneously measured stellar parameters and iron abundances are
given for all Sun-like stars selected, based on spectra of high resolution and
high signal-to-noise ratio. In addition, we measure the Li equivalent width and
abundance as well as the relative intensity of the H alpha core and the
corresponding chromospheric flux. The studied stars infer an average Ursa Major
group iron abundance of -0.03+-0.05 dex, which is higher by about 0.06 dex than
determined elsewhere. The Li abundance derived of Ursa Major group dwarf stars
is higher than in the Hyades at effective temperatures cooler than the Sun, but
lower than in the younger Pleiades, a result which is independent of the exact
value of the effective temperature adopted. The Sun-like and cooler dwarfs also
display chromospheric infilling of the H alpha core. We present spectroscopic
criteria that may be used to exclude non-members.Comment: accepted for publication in A&A, 19 pages, 10 figures, 7 table
Surface Incompressibility from Semiclassical Relativistic Mean Field Calculations
By using the scaling method and the Thomas-Fermi and Extended Thomas-Fermi
approaches to Relativistic Mean Field Theory the surface contribution to the
leptodermous expansion of the finite nuclei incompressibility has been
self-consistently computed. The validity of the simplest expansion, which
contains volume, volume-symmetry, surface and Coulomb terms, is examined by
comparing it with self-consistent results of the finite nuclei
incompressibility for some currently used non-linear sigma-omega parameter
sets. A numerical estimate of higher-order contributions to the leptodermous
expansion, namely the curvature and surface-symmetry terms, is made.Comment: 18 pages, REVTeX, 3 eps figures, changed conten
Galactomannan testing of bronchoalveolar lavage fluid is useful for diagnosis of invasive pulmonary aspergillosis in hematology patients
<p>Abstract</p> <p>Background</p> <p>Invasive pulmonary aspergillosis (IPA) is a major cause of morbidity and mortality in patients with hematological malignancies in the setting of profound neutropenia and/or hematopoietic stem cell transplantation. Early diagnosis and therapy has been shown to improve outcomes, but reaching a definitive diagnosis quickly can be problematic. Recently, galactomannan testing of bronchoalveolar lavage (BAL) fluid has been investigated as a diagnostic test for IPA, but widespread experience and consensus on optical density (OD) cut-offs remain lacking.</p> <p>Methods</p> <p>We performed a prospective case-control study to determine an optimal BAL galactomannan OD cutoff for IPA in at-risk patients with hematological diagnoses. Cases were subjects with hematological diagnoses who met established definitions for proven or probable IPA. There were two control groups: subjects with hematological diagnoses who did not meet definitions for proven or probable IPA and subjects with non-hematological diagnoses who had no evidence of aspergillosis. Following bronchoscopy and BAL, galactomannan testing was performed using the Platelia <it>Aspergillus </it>seroassay in accordance with the manufacturer's instructions.</p> <p>Results</p> <p>There were 10 cases and 52 controls. Cases had higher BAL fluid galactomannan OD indices (median 4.1, range 1.1-7.7) compared with controls (median 0.3, range 0.1-1.1). ROC analysis demonstrated an optimum OD index cutoff of 1.1, with high specificity (98.1%) and sensitivity (100%) for diagnosing IPA.</p> <p>Conclusions</p> <p>Our results also support BAL galactomannan testing as a reasonably safe test with higher sensitivity compared to serum galactomannan testing in at-risk patients with hematological diseases. A higher OD cutoff is necessary to avoid over-diagnosis of IPA, and a standardized method of collection should be established before results can be compared between centers.</p
Population Structure of Staphylococcus aureus from Remote African Babongo Pygmies
Staphylococcus aureus is a bacterium that colonizes humans worldwide. The anterior nares are its main ecological niche. Carriers of S. aureus are at a higher risk of developing invasive infections. Few reports indicated a different clonal structure and profile of virulence factors in S. aureus isolates from Sub-Saharan Africa. As there are no data about isolates from remote indigenous African populations, we conducted a cross-sectional survey of S. aureus nasal carriage in Gabonese Babongo Pygmies. The isolates were characterized regarding their susceptibility to antibiotic agents, possession of virulence factors and clonal lineage. While similar carriage rates were found in populations of industrialized countries, isolates that encode the genes for the Panton-Valentine leukocidin (PVL) were clearly more prevalent than in European countries. Of interest, many methicillin-susceptible S. aureus isolates from Babongo Pygmies showed the same genetic background as pandemic methicillin-resistant S. aureus (MRSA) clones. We advocate a surveillance of S. aureus in neglected African populations to control the development of resistance to antibiotic drugs with particular respect to MRSA and to assess the impact of the high prevalence of PVL-positive isolates
Extrasolar planet population synthesis IV. Correlations with disk metallicity, mass and lifetime
Context. This is the fourth paper in a series showing the results of planet
population synthesis calculations.
Aims. Our goal in this paper is to systematically study the effects of
important disk properties, namely disk metallicity, mass and lifetime on
fundamental planetary properties.
Methods. For a large number of protoplanetary disks we calculate a population
of planets with our core accretion formation model including planet migration
and disk evolution.
Results. We find a large number of correlations: Regarding the planetary
initial mass function, metallicity, disk mass and disk lifetime have different
roles: For high [Fe/H], giant planets are more frequent. For high disk masses,
giant planets are more massive. For long disk lifetimes, giant planets are both
more frequent and massive. At low metallicities, very massive giant planets
cannot form, but otherwise giant planet mass and metallicity are uncorrelated.
In contrast, planet masses and disk gas masses are correlated. The sweet spot
for giant planet formation is at 5 AU. In- and outside this distance, higher
planetesimals surface densities are necessary. Low metallicities can be
compensated by high disk masses, and vice versa, but not ad infinitum. At low
metallicities, giant planets only form outside the ice line, while at high
metallicities, giant planet formation occurs throughout the disk. The extent of
migration increases with disk mass and lifetime and usually decreases with
metallicity. No clear correlation of metallicity and the semimajor axis of
giant planets exists because in low [Fe/H] disks, planets start further out,
but migrate more, whereas for high [Fe/H] they start further in, but migrate
less. Close-in low mass planets have a lower mean metallicity than Hot
Jupiters.
Conclusions. The properties of protoplanetary disks are decisive for the
properties of planets, and leave many imprints.Comment: 23 pages, 16 figures. Accepted for A&
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