Indigenous knowledge in climate change adaptation: Case studies of ethnic minorities in the Northern Mountain Region of Vietnam

This study aims to investigate the indigenous knowledge (IK) of three ethnic minority groups in the Northern Mountain Region (NMR) of Vietnam. The groups include (1) Tay people who live at lower elevations; (2) a Dao community who tend to live in the middle elevations and (3) Hmong farmers who mainly reside at higher elevations areas of the mountain. This research intends to identify climate change (CC) and its impact on agricultural cultivation and find out how these groups can adapt to CC by applying their IK in agriculture practices. Data was collected through focus group discussions (n=9), in-depth interviews (n=80), and participant observation. From the 80 respondents, 27 live in Bac Kan province, 23 in Yen Bai province and 30 in Son La province; those who had experience in agricultural production, elderly and village heads. The results show that the NMR weather has significant changes that negatively impact agriculture cultivation and local livelihood. Although the respondents are from different ethnic minorities, these farmers are highly aware of the CC risks, leading into adaptation practices. While the Tay people's major adaptation strategies include the use of a variety of native plants and changing planting calendars, the Dao and Hmong people apply intercropping and local techniques methods in terracing fields using local varieties of livestock. Our findings highlight the importance of using the IK of ethnic minorities in adaptation towards CC. A better targeting about the use of local resources in future national policies and projects is encouraged.Nghiên cứu này nhằm thu thập kiến thức bản địa (IK) của ba nhóm dân tộc thiểu số ở Miền núi phía Bắc (MNPB) của Việt Nam bao gồm (1) dân tộc Tày chủ yếu sống ở vùng thấp; (2) Người Dao có xu hướng sống ở các độ cao trung bình; và (3) người Hmông chủ yếu cư trú ở các khu vực đồi núi cao. Nghiên cứu này nhằm xác định tình hình biến đổi khí hậu (BĐKH) và tác động của nó đối với sản xuất nông nghiệp, đồng thời tìm hiểu cách thức các nhóm dân tộc thiểu số này có thể thích ứng với BĐKH bằng cách áp dụng các kiến thức bản địa của họ vào thực tiễn sản xuất nông nghiệp. Dữ liệu được thu thập thông qua thảo luận nhóm tập trung (n = 9), phỏng vấn sâu (n = 80) và quan sát người tham gia. Trong số 80 người được hỏi, có 27 người sống ở tỉnh Bắc Kạn; 23 người ở tỉnh Yên Bái và 30 người ở tỉnh Sơn La, là những người có kinh nghiệm sản xuất nông nghiệp, người cao tuổi và trưởng thôn. Kết quả cho thấy thời tiết ở khu vực MNPB đã có những thay đổi so với trước gây tác động xấu đến canh tác nông nghiệp và sinh kế của cộng đồng. Mặc dù những người được hỏi từ các dân tộc khác nhau nhưng họ đều nhận thức được sự thay đổi này của thời tiết, do đó họ đã có những thích ứng riêng. Trong khi người Tày sử dụng giống cây trồng địa phương và thay đổi lịch thời vụ thì người Dao và Hmong chọn phương pháp xen canh và áp dụng kỹ thuật bản địa trên đất ruộng bậc thang và sử dụng gióng vật nuôi bản địa. Các phát hiện của chúng tôi giúp hiểu được tầm quan trọng của việc sử dụng IK trong thích ứng với BĐKH của các dân tộc thiểu số, từ đó có thể hướng đến mục tiêu tốt hơn việc sử dụng các nguồn lực địa phương trong các chính sách và dự án quốc gia trong tương lai

Secondary metabolites from Micromonospora ectrinospora G017

Eight  compounds, cyclo-(Pro-Tryp) (1), N-[2-(1H-indol-3-yl)-2-oxo-ethyl] acetamide (2), cyclo-(Pro-Tyr) (3), cyclo-(Pro-Phe) (4), cyclo-trans-4-OH-(Pro-Phe) (5), cyclo-(Pro-Leu) (6), cyclo-(Pro-Val) (7), and  uracil (8) were isolated from the culture broth of the marine Micromonospora ectrinospora G017 strain. The structures of the isolated compounds were established on the basis of their spectral data, including mass spectrometry and NMR

SECONDARY METABOLITES FROM MICROMONOSPORA SP. (G044)

In the course of our screening program, the EtOAc extract of a Micromonospora sp. (strain G044) from sponge Tethya aurantium of the sea of Côtô - Thanh Lân exhibited antimicrobial activity against Enterococcus faecalis, Bacillus cereus and Candida albicans. In this paper, we reported the isolation and structural elucidation of six secondary metabolites Cyclo-(Pro-Trp) (1), Cyclo-(Pro-Met) (2), Cyclo-(Pro-Val) (4), N-acetyltryptamine (3), uridine (5), and 2-phenylacetic acid (6) from the cultures broth of Micromonospora sp. (strain G044). The structures of 1 – 6 were determined by analyses of MS and 2D NMR data. All compounds were evaluated for their antimicrobial activity against a panel of clinically significant microorganisms. Compound 1 inhibited Escherichia coli with a MIC value of 128 µg/ml

Terpenoids from leaves of Viburnum sambucinum Reinw. ex. Blume (Caprifoliaceae)

Six compounds were isolated from the leaves of Viburnum sambucinum Reinw. ex Blume (Caprifoliaceae).  The structures of the isolates were determined by spectroscopic analysis including MS and NMR. Accordingly, the isolated compounds were identified as a-amyrin (1), ursolic acid (2), 3β,28-dihydroxy-urs-12-ene (3), oleanolic acid (4), 16β-hydroxylup-20(29)-ene-3-one (5) and trans-2-phyten-1-ol (6)

Differential Infectivity of Human Neural Cell Lines by a Dengue Virus Serotype-3 Genotype-III with a Distinct Nonstructural Protein 2A (NS2A) Amino Acid Substitution Isolated from the Cerebrospinal Fluid of a Dengue Encephalitis Patient

Dengue encephalitis is considered as a severe but unusual clinical presentation of dengue infection. Limited molecular information is available on the neurotropism of dengue virus (DENV), highlighting the need for further research. During a dengue outbreak in Vietnam in 2013, two DENV-3 strains were isolated, in which one was isolated from cerebrospinal fluid (CSF) samples from a dengue encephalitis patient and another strain was isolated from a patient with classical dengue fever in Hai Phong, Vietnam. DENV serotype-3 (DENV-3) isolated from these samples belonged to genotype III, marking the first report of this genotype in the country at that time. Genetic variation between both strains was elucidated by using a full genome sequencing by next-generation sequencing (NGS). The infectivity of the isolated DENV-3 strains was further characterized using human and mouse neuronal cell lines. Phylogenetic analysis of the isolates demonstrated high homogeneity between the CSF-derived and serum-derived DENV-3, in which the full genome sequences of the CSF-derived DENV-3 presented a Thr-1339-Ile mutation in the nonstructural 2A (NS2A) protein. The CSF-derived DENV-3 isolate grew preferentially in human neuronal cells, with a significant proportion of cells that were positive for nonstructural 1 (NS1), nonstructural 4B (NS4B), and nonstructural 5 (NS5) antigens. These results suggest that NS2A may be a crucial region in the neuropathogenesis of DENV-3 and its growth in human neuronal cells. Taken together, our results demonstrate that a CSF-derived DENV-3 has unique infectivity characteristics for human neuronal cells, which might play a crucial role in the neuropathogenesis of DENV infection

Safety and efficacy of fluoxetine on functional outcome after acute stroke (AFFINITY): a randomised, double-blind, placebo-controlled trial

Background Trials of fluoxetine for recovery after stroke report conflicting results. The Assessment oF FluoxetINe In sTroke recoverY (AFFINITY) trial aimed to show if daily oral fluoxetine for 6 months after stroke improves functional outcome in an ethnically diverse population. Methods AFFINITY was a randomised, parallel-group, double-blind, placebo-controlled trial done in 43 hospital stroke units in Australia (n=29), New Zealand (four), and Vietnam (ten). Eligible patients were adults (aged ≥18 years) with a clinical diagnosis of acute stroke in the previous 2–15 days, brain imaging consistent with ischaemic or haemorrhagic stroke, and a persisting neurological deficit that produced a modified Rankin Scale (mRS) score of 1 or more. Patients were randomly assigned 1:1 via a web-based system using a minimisation algorithm to once daily, oral fluoxetine 20 mg capsules or matching placebo for 6 months. Patients, carers, investigators, and outcome assessors were masked to the treatment allocation. The primary outcome was functional status, measured by the mRS, at 6 months. The primary analysis was an ordinal logistic regression of the mRS at 6 months, adjusted for minimisation variables. Primary and safety analyses were done according to the patient's treatment allocation. The trial is registered with the Australian New Zealand Clinical Trials Registry, ACTRN12611000774921. Findings Between Jan 11, 2013, and June 30, 2019, 1280 patients were recruited in Australia (n=532), New Zealand (n=42), and Vietnam (n=706), of whom 642 were randomly assigned to fluoxetine and 638 were randomly assigned to placebo. Mean duration of trial treatment was 167 days (SD 48·1). At 6 months, mRS data were available in 624 (97%) patients in the fluoxetine group and 632 (99%) in the placebo group. The distribution of mRS categories was similar in the fluoxetine and placebo groups (adjusted common odds ratio 0·94, 95% CI 0·76–1·15; p=0·53). Compared with patients in the placebo group, patients in the fluoxetine group had more falls (20 [3%] vs seven [1%]; p=0·018), bone fractures (19 [3%] vs six [1%]; p=0·014), and epileptic seizures (ten [2%] vs two [<1%]; p=0·038) at 6 months. Interpretation Oral fluoxetine 20 mg daily for 6 months after acute stroke did not improve functional outcome and increased the risk of falls, bone fractures, and epileptic seizures. These results do not support the use of fluoxetine to improve functional outcome after stroke