Advance Interoperability in e-Government with Standardized Core Directories

Many new requirements for the public sector arise from the change of the society and the “Age of Knowledge”. Globalisation leads administrations to become more interoperable, irrespective of national borders. To meet these requirements, administrations and IT systems need to become more efficient. One of the main principles in software engineering is reusability. This can be applied on all levels. There is still a lot of potential at the content level. The idea of reusing content is not new but especially in federal structures where central solutions are nearly impossible this is a great challenge. The document describes an approach that allows information to be collected in a decentralised way and makes it available in an interdisciplinary manner and across regional borders. Core Directories will be designed and used as an infrastructural component to make them accessible for multiple applications. In order to share information, data interoperability standards are needed

On the Applicability of Payment Channel Networks for Allocation of Transport Ticket Revenues

In many public transport networks, multiple providers cooperate to offer integrated services and, consequently, provide integrated fare collection. Thus, ticket revenues need to be redistributed so that each provider receives its respective share. Typically, the customers’ travel behavior is surveyed and the fares paid are aggregated over certain periods of time, and the revenue is periodically allocated based on this information. To avoid a centralized trusted third party or the exchange of sensitive information between providers, we present an approach that integrates revenue allocation directly into the payment process: The proposed approach is based on payment channel networks and utilizes multi-hop payments to perform revenue allocation. We thereby show how to make use of payment channel networks in this setting as well as the corresponding benefits

Peptides encoded by short ORFs control development and define a new eukaryotic gene family

Despite recent advances in developmental biology and in genomics, key questions remain regarding the organisation of cells into embryos. One possibility is that novel types of genes might await discovery and could provide some of the answers. Genome annotation depends strongly on comparison with previously known gene sequences, and so genes having previously uncharacterised structure and function can be missed. Here we present the characterisation of tarsal-less, a new such type of gene. Tarsal-less has two unusual features: first, it contains more than one coding unit, a structure more similar to some bacterial genes. Second, it codes for small peptides rather than proteins, and in fact these peptides represent the smallest gene products known to date. Functional analysis of this gene in the fruitfly Drosophila shows that it has important functions throughout development, including tissue morphogenesis and pattern formation. We identify genes similar to tarsal-less in other species, and thus define a tarsal-less-related gene family. We expect that a combination of bioinformatic and functional methods, such as the ones we use in this study, will identify and characterize more genes of this type. Potentially, thousands of such new genes may exist

An energy budget agent-based model of earthworm populations and its application to study the effects of pesticides

Earthworms are important organisms in soil communities and so are used as model organisms in environ-mental risk assessments of chemicals. However current risk assessments of soil invertebrates are based on short-term laboratory studies, of limited ecological relevance, supplemented if necessary by site-specific field trials, which sometimes are challenging to apply across the whole agricultural landscape. Here, we investigate whether population responses to environmental stressors and pesticide exposure can be accurately predicted by combining energy budget and agent-based models (ABMs), based on knowledge of how individuals respond to their local circumstances. A simple energy budget model was implemented within each earthworm Eisenia fetida in the ABM, based on a priori parameter estimates .From broadly accepted physiological principles, simple algorithms specify how energy acquisition and expenditure drive life cycle processes. Each individual allocates energy between maintenance, growth and/or reproduction under varying conditions of food density, soil temperature and soil moisture. When simulating published experiments, good model fits were obtained to experimental data on individual growth, reproduction and starvation. Using the energy budget model as a platform we developed methods to identify which of the physiological parameters in the energy budget model (rates of ingestion, maintenance, growth or reproduction) are primarily affected by pesticide applications, producing four hypotheses about how toxicity acts. We tested these hypotheses by comparing model outputs with published toxicity data on the effects of copper oxychloride and chlorpyrifos on E. fetida. Both growth and reproduction were directly affected in experiments in which sufficient food was provided, whilst maintenance was targeted under food limitation. Although we only incorporate toxic effects at the individual level we show how ABMs can readily extrapolate to larger scales by providing good model fits to field population data. The ability of the presented model to fit the available field and laboratory data for E.fetida demonstrates the promise of the agent-based approach in ecology, by showing how biological knowledge can be used to make ecological inferences. Further work is required to extend the approach to populations of more ecologically relevant species studied at the field scale. Such a model could help extrapolate from laboratory to field conditions and from one set of field conditions to another or from species to species

The Complex Spatio-Temporal Regulation of the Drosophila Myoblast Attractant Gene duf/kirre

A key early player in the regulation of myoblast fusion is the gene dumbfounded (duf, also known as kirre). Duf must be expressed, and function, in founder cells (FCs). A fixed number of FCs are chosen from a pool of equivalent myoblasts and serve to attract fusion-competent myoblasts (FCMs) to fuse with them to form a multinucleate muscle-fibre. The spatial and temporal regulation of duf expression and function are important and play a deciding role in choice of fibre number, location and perhaps size. We have used a combination of bioinformatics and functional enhancer deletion approaches to understand the regulation of duf. By transgenic enhancer-reporter deletion analysis of the duf regulatory region, we found that several distinct enhancer modules regulate duf expression in specific muscle founders of the embryo and the adult. In addition to existing bioinformatics tools, we used a new program for analysis of regulatory sequence, PhyloGibbs-MP, whose development was largely motivated by the requirements of this work. The results complement our deletion analysis by identifying transcription factors whose predicted binding regions match with our deletion constructs. Experimental evidence for the relevance of some of these TF binding sites comes from available ChIP-on-chip from the literature, and from our analysis of localization of myogenic transcription factors with duf enhancer reporter gene expression. Our results demonstrate the complex regulation in each founder cell of a gene that is expressed in all founder cells. They provide evidence for transcriptional control—both activation and repression—as an important player in the regulation of myoblast fusion. The set of enhancer constructs generated will be valuable in identifying novel trans-acting factor-binding sites and chromatin regulation during myoblast fusion in Drosophila. Our results and the bioinformatics tools developed provide a basis for the study of the transcriptional regulation of other complex genes

Advance Interoperability in e-Government with standardised Core Directories

Many new requirements for the public sector arise from the change of the society and the "Age of Knowledge". Globalisation leads administrations to become more interoperable, irrespective of national borders. To meet these requirements, administrations and IT systems need to become more efficient. One of the main principles in software engineering is reusability. This can be applied on all levels. There is still a lot of potential at the content level. The idea of reusing content is not new but especially in federal structures where central solutions are nearly impossible this is a great challenge. The document describes an approach that allows information to be collected in a decentralised way and makes it available in an interdisciplinary manner and across regional borders. Core Directories will be designed and used as an infrastructural component to make them accessible for multiple applications. In order to share information, data interoperability standards are needed