Pushing forward white lupin as a local source for protein and nitrogen in Central Europe

White lupin (Lupinus albus L.) is a promising leguminous crop. Europe is fully dependent on protein and nitrogen fertilizer imports. This has tremendous negative effects both in Europe and the producing countries, such as loss of terrestrial biodiversity, pollution of freshwater, increase of greenhouse gases and soil acidification. Diverse crop-rotations with a substantial amount of pulses are a proven solution. The protein composition and yield potential of white lupin suggest that it could become the ‘Soy of the North’. Currently, the seed-borne pathogen Colletotrichum lupini is substantially impeding the cultivation of white lupin in Central Europe. We developed a DNA-based diagnostic test to identify and quantify the fungal pathogen in plants and seeds. This technique will allow us to improve our understanding of the Colletotrichum lupini life cycle and, thereby, lay the basis for an advanced resistance breeding approach

Suppression or Activation of Immune Responses by Predicted Secreted Proteins of the Soybean Rust Pathogen Phakopsora pachyrhizi

Rust fungi, such as the soybean rust pathogen Phakopsora pachyrhizi, are major threats to crop production. They form specialized haustoria that are hyphal structures intimately associated with host-plant cell membranes. These haustoria have roles in acquiring nutrients and secreting effector proteins that manipulate host immune systems. Functional characterization of effector proteins of rust fungi is important for understanding mechanisms that underlie their virulence and pathogenicity. Hundreds of candidate effector proteins have been predicted for rust pathogens, but it is not clear how to prioritize these effector candidates for further characterization. There is a need for high-throughput approaches for screening effector candidates to obtain experimental evidence for effector-like functions, such as the manipulation of host immune systems. We have focused on identifying effector candidates with immune-related functions in the soybean rust fungus P. pachyrhizi. To facilitate the screening of many P. pachyrhizi effector candidates (named PpECs), we used heterologous expression systems, including the bacterial type III secretion system, Agrobacterium infiltration, a plant virus, and a yeast strain, to establish an experimental pipeline for identifying PpECs with immune-related functions and establishing their subcellular localizations. Several PpECs were identified that could suppress or activate immune responses in nonhost Nicotiana benthamiana, N. tabacum, Arabidopsis, tomato, or pepper plants

The haustorial transcriptomes of Uromyces appendiculatus and Phakopsora pachyrhizi and their candidate effector families

Haustoria of biotrophic rust fungi are responsible for the uptake of nutrients from their hosts and for the production of secreted proteins, known as effectors, which modulate the host immune system. The identification of the transcriptome of haustoria and an understanding of the functions of expressed genes therefore hold essential keys for the elucidation of fungus–plant interactions and the development of novel fungal control strategies. Here, we purified haustoria from infected leaves and used 454 sequencing to examine the haustorial transcriptomes of Phakopsora pachyrhizi and Uromyces appendiculatus, the causal agents of soybean rust and common bean rust, respectively. These pathogens cause extensive yield losses in their respective legume crop hosts. A series of analyses were used to annotate expressed sequences, including transposable elements and viruses, to predict secreted proteins from the assembled sequences and to identify families of candidate effectors. This work provides a foundation for the comparative analysis of haustorial gene expression with further insights into physiology and effector evolution

Analysis of the pore of the unusual major intrinsic protein channel, yeast Fps1p

Fps1p is a glycerol efflux channel from Saccharomyces cerevisiae. In this atypical major intrinsic protein neither of the signature NPA motifs of the family, which are part of the pore, is preserved. To understand the functional consequences of this feature, we analyzed the pseudo-NPA motifs of Fps1p by site-directed mutagenesis and assayed the resultant mutant proteins in vivo. In addition, we took advantage of the fact that the closest bacterial homolog of Fps1p, Escherichia coli GlpF, can be functionally expressed in yeast, thus enabling the analysis in yeast cells of mutations that make this typical major intrinsic protein more similar to Fps1p. We observed that mutations made in Fps1p to "restore" the signature NPA motifs did not substantially affect channel function. In contrast, when GlpF was mutated to resemble Fps1p, all mutants had reduced activity compared with wild type. We rationalized these data by constructing models of one GlpF mutant and of the transmembrane core of Fps1p. Our model predicts that the pore of Fps1p is more flexible than that of GlpF. We discuss the fact that this may accommodate the divergent NPA motifs of Fps1p and that the different pore structures of Fps1p and GlpF may reflect the physiological roles of the two glycerol facilitators

Food losses from farm to retail operations: agricultural produces supply chain of Baja Peninsula, México

Objective: To evaluate food losses (FL) volumes generated by farms in Baja California Peninsula, México, of five agricultural commodities. Design/methodology/approach: Baja California Sur (BCS) state was the study area. Information was gathered from a total of 380 sampled chain actors in asparagus, mango, strawberry, orange and tomato by survey and personal interviews. Tobit technique was applied to identify factors that influence FL percentage. Results: Data shows about 11.8% of asparagus is lost during harvesting and distribution, as well as 8.5% of strawberry, 26% of mango, 17.8% of oranges and 3.5% of tomatoes, representing 29.9% loss rate of marketed yield. Limitations on study/implications: This study did not classify commodities in the last steps of the supply chain. The five commodities used in the current study correspond to the more important agricultural produces in BCS, but given changing market, harvesting time and produce availability did not consider the waste of the supply chain. Findings/conclusions: Commodity, type of transportation and distribution, education, and human resources has been identified as influence factors in the volume of FL. This exploratory study fills the void in information in terms of its geographic scope and food group number, and farm owners willing to manage food losses for the purpose of obtaining bioactive compound. Keywords: Food loss, agrifood, desert agriculture, food security, retailObjective: To evaluate food losses (FL) volumes generated by farms in Baja California Peninsula, México, of five agricultural commodities. Design/methodology/approach: Baja California Sur (BCS) state was the study area. Information was gathered from a total of 380 sampled chain actors in asparagus, mango, strawberry, orange and tomato by survey and personal interviews. Tobit technique was applied to identify factors that influence FL percentage. Results: Data shows about 11.8% of asparagus is lost during harvesting and distribution, as well as 8.5% of strawberry, 26% of mango, 17.8% of oranges and 3.5% of tomatoes, representing 29.9% loss rate of marketed yield. Limitations on study/implications: This study did not classify commodities in the last steps of the supply chain. The five commodities used in the current study correspond to the more important agricultural produces in BCS, but given changing market, harvesting time and produce availability did not consider the waste of the supply chain. Findings/conclusions: Commodity, type of transportation and distribution, education, and human resources has been identified as influence factors in the volume of FL. This exploratory study fills the void in information in terms of its geographic scope and food group number, and farm owners willing to manage food losses for the purpose of obtaining bioactive compound

Corob-x: a cooperative robot team for the exploration of lunar skylights

The project CoRob-X develops and demonstrates enabling technologies for multi-agent robotic teams to explore planetary surfaces with a focus on hard-to-reach areas where a collaborative scheme is required to efficiently explore complex environments. Exploring lava tubes is such a challenging environment and requires a team of robots able to collaborate in an autonomous way to find their way to the subsurface tube system, descend through a natural entry hole (the so-called skylight), and explore the interior with payload instruments to provide scientific data. The developed robotic exploration system that will tackle the ambitious goal is composed of three rovers with substantially different technical characteristics. The paper presents the overall approach, i.e., the control architecture, the robotic systems, and the software to be used. It also showcases the selected mission phases that will be demonstrated in a field-test campaign. In addition, a terrestrial mining use case is presented that demonstrates how the developed autonomy-enabling software can be transferred to terrestrial applications.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Integrative and comparative genomic analyses identify clinically relevant pulmonary carcinoid groups and unveil the supra-carcinoids

International audienceThe worldwide incidence of pulmonary carcinoids is increasing, but little is known about their molecular characteristics. Through machine learning and multi-omics factor analysis, we compare and contrast the genomic profiles of 116 pulmonary carcinoids (including 35 atypical), 75 large-cell neuroendocrine carcinomas (LCNEC), and 66 small-cell lung cancers. Here we report that the integrative analyses on 257 lung neuroendocrine neoplasms stratify atypical carcinoids into two prognostic groups with a 10-year overall survival of 88% and 27%, respectively. We identify therapeutically relevant molecular groups of pulmonary car-cinoids, suggesting DLL3 and the immune system as candidate therapeutic targets; we confirm the value of OTP expression levels for the prognosis and diagnosis of these diseases, and we unveil the group of supra-carcinoids. This group comprises samples with carcinoid-like morphology yet the molecular and clinical features of the deadly LCNEC, further supporting the previously proposed molecular link between the low-and high-grade lung neuroendocrine neoplasms