Evaluación molecular de la presencia de Perkinsus spp en muestras de concha de abanico (Argopecten purpuratus) del norte del Perú

The aim of this study was to evaluate the presence of Perkinsus spp, a parasite that is mandatory to be report to the OIE, in samples of calico scallop (Argopecten purpuratus) from Ancash, Peru, using the PCR technique. In total, 56 samples were selected from those collected for a previous study in 2015. The selected samples were gill tissues with histological lesions suggestive of infection with the protozoan (hemocytic infiltration and necrosis). DNA was extracted from the samples and specific primers were used to detect Perkinsus spp by PCR. In addition, the detection limit of the technique was evaluated. All samples were negative. It was determined that 1.63 ng was the least detectable template DNA in one of the positive controls.El objetivo de este estudio fue evaluar la presencia de Perkinsus spp, parásito de reporte obligatorio ante la OIE, en muestras de concha de abanico (Argopecten purpuratus) de Ancash, Perú, mediante la técnica de PCR. Se seleccionaron 56 muestras de las colectadas para un estudio previo en 2015. Las muestras seleccionadas fueron tejidos de branquia con lesiones histológicas sugestivas de infección con el protozoo (infiltración hemocítica y necrosis). Se extrajo el ADN de las muestras y se utilizaron cebadores específicos para detectar Perkinsus spp por PCR. Además, se evaluó el límite de detección de la técnica. Todas las muestras resultaron negativas. Se determinó que 1.63 ng fue la menor cantidad de DNA molde detectable en uno de los controles positivos

Open data from the first and second observing runs of Advanced LIGO and Advanced Virgo

Advanced LIGO and Advanced Virgo are monitoring the sky and collecting gravitational-wave strain data with sufficient sensitivity to detect signals routinely. In this paper we describe the data recorded by these instruments during their first and second observing runs. The main data products are gravitational-wave strain time series sampled at 16384 Hz. The datasets that include this strain measurement can be freely accessed through the Gravitational Wave Open Science Center at http://gw-openscience.org, together with data-quality information essential for the analysis of LIGO and Virgo data, documentation, tutorials, and supporting software