258 research outputs found
Desyndactyly of 4th and 5th toe with autologus skin graft of ankle
For a good surgical treatment of desyndactyly a good presurgical planning must be made to decrease the complications intra and post surgical. It is important to inform to the patient the surgical treatment purpouse and to leave in a background the aesthetic result due to the patient can feel fear and unconformed with the scars.For a good surgical treatment of desyndactyly a good presurgical planning must be made to decrease the complications intra and post surgical. It is important to inform to the patient the surgical treatment purpouse and to leave in a background the aesthetic result due to the patient can feel fear and unconformed with the scars
Cross-class metallo-ÎČ-lactamase inhibition by bisthiazolidines reveals multiple binding modes
Metallo-ÎČ-lactamases (MBLs) hydrolyze almost all ÎČ-lactam antibiotics and are unaffected by clinically available ÎČ-lactamase inhibitors (ÎČLIs). Active-site architecture divides MBLs into three classes (B1, B2, and B3), complicating development of ÎČLIs effective against all enzymes. Bisthiazolidines (BTZs) are carboxylate-containing, bicyclic compounds, considered as penicillin analogs with an additional free thiol. Here, we show both L- and D-BTZ enantiomers are micromolar competitive ÎČLIs of all MBL classes in vitro, with Ki sof6-15 ÎŒM or 36-84 ÎŒM for subclass B1 MBLs (IMP-1 and BcII, respectively), and 10-12 ÎŒM for the B3 enzyme L1. Against the B2 MBL Sfh-I, the L-BTZ enantiomers exhibit 100-fold lower Ki s (0.26-0.36 ÎŒM) than D-BTZs (26-29 ÎŒM). Importantly, cell-based time-kill assays show BTZs restore ÎČ-lactam susceptibility of Escherichia coli-producing MBLs (IMP-1, Sfh-1, BcII, and GOB-18) and, significantly, an extensively drug-resistant Stenotrophomonas maltophilia clinical isolate expressing L1. BTZs therefore inhibit the full range of MBLs and potentiate ÎČ-lactam activity against producer pathogens. X-ray crystal structures reveal insights into diverse BTZ binding modes, varying with orientation of the carboxylate and thiol moieties. BTZs bind the di-zinc centers of B1 (IMP-1; BcII) and B3 (L1) MBLs via the free thiol, but orient differently depending upon stereochemistry. In contrast, the L-BTZ carboxylate dominates interactions with the monozinc B2 MBL Sfh-I, with the thiol uninvolved. D-BTZ complexes most closely resemble ÎČ-lactam binding to B1 MBLs, but feature an unprecedented disruption of the D120-zinc interaction. Cross-class MBL inhibition therefore arises from the unexpected versatility of BTZ binding.Fil: Hinchliffe, Philip. University of Bristol; Reino UnidoFil: Gonzalez, Javier Marcelo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Rosario. Instituto de BiologĂa Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias BioquĂmicas y FarmacĂ©uticas. Instituto de BiologĂa Molecular y Celular de Rosario; ArgentinaFil: Mojica, MarĂa. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University; Estados UnidosFil: Gonzalez, Javier Marcelo. Universidad Nacional de Santiago del Estero. Instituto de BionanotecnologĂa del Noa. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - TucumĂĄn. Instituto de BionanotecnologĂa del Noa; ArgentinaFil: Castillo, Valerie. Universidad de la RepĂșblica; UruguayFil: Saiz Garcia, Cecilia. Universidad de la RepĂșblica; UruguayFil: Kosmopoulou, Magda. University of Bristol; Reino UnidoFil: Tooke, Catherine. University of Bristol; Reino UnidoFil: Llarrull, Leticia Irene. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Rosario. Instituto de BiologĂa Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias BioquĂmicas y FarmacĂ©uticas. Instituto de BiologĂa Molecular y Celular de Rosario; ArgentinaFil: Mahler, Graciela. Universidad de la RepĂșblica; UruguayFil: Bonomo, Robert. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University; Estados UnidosFil: Vila, Alejandro Jose. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Rosario. Instituto de BiologĂa Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias BioquĂmicas y FarmacĂ©uticas. Instituto de BiologĂa Molecular y Celular de Rosario; ArgentinaFil: Spencer, James. University of Bristol; Reino Unid
The Role of Computational Fluid Dynamics in the Management of Unruptured Intracranial Aneurysms: A Clinicians' View
Objective. The
importance of hemodynamics in the
etiopathogenesis of intracranial aneurysms (IAs)
is widely accepted. Computational fluid dynamics
(CFD) is being used increasingly for hemodynamic
predictions. However, alogn with the continuing
development and validation of these
tools, it is imperative to collect
the opinion of the clinicians.
Methods. A workshop on CFD was
conducted during the European Society of
Minimally Invasive Neurological Therapy (ESMINT)
Teaching Course, Lisbon, Portugal.
36 delegates, mostly clinicians,
performed supervised CFD analysis for an IA, using the
@neuFuse software developed within the European
project @neurIST. Feedback on the workshop was
collected and analyzed. The
performance was assessed on a scale of 1 to 4
and, compared with experts' performance.
Results. Current dilemmas in
the management of unruptured IAs remained the
most important motivating factor to attend the
workshop and majority of participants showed interest in participating in a
multicentric trial. The participants achieved
an average score of 2.52 (range 0â4) which was 63% (range 0â100%) of an expert user. Conclusions.
Although participants showed a manifest interest
in CFD, there was a clear
lack of awareness concerning the role of
hemodynamics in the etiopathogenesis of IAs and
the use of CFD in this context. More efforts
therefore are required to enhance understanding of the
clinicians in the subject
Flow complexity in open systems: interlacing complexity index based on mutual information
Flow complexity is related to a number of phenomena in science and engineering and has been approached from the perspective of chaotic dynamical systems, ergodic processes or mixing of fluids, just to name a few. To the best of our knowledge, all existing methods to quantify flow complexity are only valid for infinite time evolution, for closed systems or for mixing of two substances. We introduce an index of flow complexity coined interlacing complexity index (ICI), valid for a single-phase flow in an open system with inlet and outlet regions, involving finite times. ICI is based on Shannonâs mutual information (MI), and inspired by an analogy between inletâoutlet open flow systems and communication systems in communication theory. The roles of transmitter, receiver and communication channel are played, respectively, by the inlet, the outlet and the flow transport between them. A perfectly laminar flow in a straight tube can be compared to an ideal communication channel where the transmitted and received messages are identical and hence the MI between input and output is maximal. For more complex flows, generated by more intricate conditions or geometries, the ability to discriminate the outlet position by knowing the inlet position is decreased, reducing the corresponding MI. The behaviour of the ICI has been tested with numerical experiments on diverse flows cases. The results indicate that the ICI provides a sensitive complexity measure with intuitive interpretation in a diversity of conditions and in agreement with other observations, such as Dean vortices and subjective visual assessments. As a crucial component of the ICI formulation, we also introduce the natural distribution of streamlines and the natural distribution of world-lines, with invariance properties with respect to the cross-section used to parameterize them, valid for any type of mass-preserving flow
San Pedro de la Mata (Sonseca, Toledo). Construir y decorar una iglesia altomedieval en piedra
This work aims to offer those results obtained by means of the archaeological, stylistic and geological analysis of the church of San Pedro de La Mata, of its building and decorative materials and of its quarries. Combining these studies (and methodologies) has made possible to identify the original form of the church, to pinpoint the origin of the materials and to characterize thus the skills of the workshops responsible for its construction and decoration.Este trabajo pretende ofrecer los primeros resultados obtenidos mediante el anĂĄlisis arqueolĂłgico, estilĂstico y geolĂłgico de la iglesia de San Pedro de La Mata, de sus materiales constructivos y decorativos y de sus canteras. La combinaciĂłn de estos estudios (y metodologĂas) ha permitido reconocer la forma originaria de la iglesia, determinar la procedencia de sus materiales y caracterizar asĂ la habilidad de los talleres responsables de su obra y decoraciĂłn
Taxonomic review of Colombian Parodon (Characiformes: Parodontidae), with descriptions of three new species
Canine Mammary Cancer Stem Cells are Radio- and Chemo- Resistant and Exhibit an Epithelial-Mesenchymal Transition Phenotype
Canine mammary carcinoma is the most common cancer among female dogs and is often fatal due to the development of distant metastases. In humans, solid tumors are made up of heterogeneous cell populations, which perform different roles in the tumor economy. A small subset of tumor cells can hold or acquire stem cell characteristics, enabling them to drive tumor growth, recurrence and metastasis. In veterinary medicine, the molecular drivers of canine mammary carcinoma are as yet undefined. Here we report that putative cancer stem cells (CSCs) can be isolated form a canine mammary carcinoma cell line, REM134. We show that these cells have an increased ability to form tumorspheres, a characteristic of stem cells, and that they express embryonic stem cell markers associated with pluripotency. Moreover, canine CSCs are relatively resistant to the cytotoxic effects of common chemotherapeutic drugs and ionizing radiation, indicating that failure of clinical therapy to eradicate canine mammary cancer may be due to the survival of CSCs. The epithelial to mesenchymal transition (EMT) has been associated with cancer invasion, metastasis, and the acquisition of stem cell characteristics. Our results show that canine CSCs predominantly express mesenchymal markers and are more invasive than parental cells, indicating that these cells have a mesenchymal phenotype. Furthermore, we show that canine mammary cancer cells can be induced to undergo EMT by TGFÎČ and that these cells have an increased ability to form tumorspheres. Our findings indicate that EMT induction can enrich for cells with CSC properties, and provide further insight into canine CSC biology
Multicentre observational study on multisystem inflammatory syndrome related to COVID-19 in Argentina
Background: The impact of the pediatric inflammatory multisystem syndrome temporally associated with SARS-CoV-2 (PIMS-TS) in low- and middle-income countries remains poorly understood. Our aim was to understand the characteristics and outcomes of PIMS-TS in Argentina. Methods: This observational, prospective, and retrospective multicenter study enrolled patients younger than 18 years-old manifesting PIMS-TS, Kawasaki disease (KD) or Kawasaki shock syndrome (KSS) between March 2020 and May 2021. Patients were followed-up until hospital discharge or death (one case). The primary outcome was pediatric intensive care unit (PICU) admission. Multiple logistic regression was used to identify variables predicting PICU admission. Results: Eighty-one percent, 82%, and 14% of the 176 enrolled patients fulfilled the suspect case criteria for PIMS-TS, KD, and KSS, respectively. Temporal association with SARS-CoV-2 was confirmed in 85% of the patients and 38% were admitted to the PICU. The more common clinical manifestations were fever, abdominal pain, rash, and conjunctival injection. Lymphopenia was more common among PICU-admitted patients (87% vs. 51%, p < 0.0001), who also showed a lower platelet count and higher plasmatic levels of inflammatory and cardiac markers. Mitral valve insufficiency, left ventricular wall motion alterations, pericardial effusion, and coronary artery alterations were observed in 30%, 30%, 19.8%, and 18.6% of the patients, respectively. Days to initiation of treatment, rash, lymphopenia, and low platelet count were significant independent contributions to PICU admission. Conclusion: Rates of severe outcomes of PIMS-TS in the present study agreed with those observed in high-income countries. Together with other published studies, this work helps clinicians to better understand this novel clinical entity.Fil: Vainstein, Eduardo. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo GutiĂ©rrez"; ArgentinaFil: Baleani, Silvia. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo GutiĂ©rrez"; ArgentinaFil: Urrutia, Luis. Gobierno de la Ciudad de Buenos Aires. Hospital de PediatrĂa "Juan P. Garrahan"; ArgentinaFil: Affranchino, NicolĂĄs. Gobierno de la Ciudad de Buenos Aires. Hospital de PediatrĂa "Juan P. Garrahan"; ArgentinaFil: Ackerman, Judith. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Cazalas, Mariana. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo GutiĂ©rrez"; ArgentinaFil: Goldsman, Alejandro. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo GutiĂ©rrez"; ArgentinaFil: Sardella, Angela. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo GutiĂ©rrez"; ArgentinaFil: Tolin, Ana Laura. Gobierno de la Provincia de Mendoza. Hospital PediĂĄtrico Humberto Notti; ArgentinaFil: Goldaracena, Pablo. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños "Sor MarĂa Ludovica" de La Plata; ArgentinaFil: Fabi, Mariana. Provincia de Buenos Aires. Ministerio de Salud. Hospital de Niños "Sor MarĂa Ludovica" de La Plata; ArgentinaFil: Cosentino, Mariana. Hospital BritĂĄnico de Buenos Aires; ArgentinaFil: Magliola, Ricardo. Hospital BritĂĄnico de Buenos Aires; ArgentinaFil: Roggiero, Gustavo. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. NĂ©stor Carlos Kirchner Samic; ArgentinaFil: Manso, Paula. Provincia de Buenos Aires. Ministerio de Salud. Hospital Alta Complejidad en Red El Cruce Dr. NĂ©stor Carlos Kirchner Samic; ArgentinaFil: Triguy, JĂ©sica. Gobierno de la Provincia de Mendoza. Hospital PediĂĄtrico Humberto Notti; ArgentinaFil: Ballester, Celeste. Gobierno de la Provincia de Mendoza. Hospital PediĂĄtrico Humberto Notti; ArgentinaFil: Cervetto, Vanesa. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños Pedro Elizalde (ex Casa Cuna); ArgentinaFil: Vaccarello, MarĂa. Sanatorio de la Trinidad; ArgentinaFil: De Carli, Domingo Norberto. ClĂnica del Niño de Quilmes; ArgentinaFil: De Carli, Maria Estela. ClĂnica del Niño de Quilmes; ArgentinaFil: Ciotti, Ana Laura. Hospital Nacional Profesor Alejandro Posadas; ArgentinaFil: Sicurello, MarĂa Irene. Gobierno de la Ciudad de Buenos Aires. Hospital General de Niños "Ricardo GutiĂ©rrez"; ArgentinaFil: Rios Leiva, Cecilia. Provincia de Buenos Aires. Ministerio de Salud. Hospital Interzonal de Agudos "Eva PerĂłn"; ArgentinaFil: Villalba, Claudia. Gobierno de la Ciudad de Buenos Aires. Hospital de PediatrĂa "Juan P. Garrahan"; ArgentinaFil: Hortas, MarĂa. Sanatorio de la Trinidad; ArgentinaFil: Peña, Sonia. Gobierno de la Provincia de Mendoza. Hospital PediĂĄtrico Humberto Notti; ArgentinaFil: GonzĂĄlez, Gabriela. Gobierno de la Provincia de Mendoza. Hospital PediĂĄtrico Humberto Notti; ArgentinaFil: Zold, Camila Lidia. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Houssay. Instituto de FisiologĂa y BiofĂsica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de FisiologĂa y BiofĂsica Bernardo Houssay; ArgentinaFil: Murer, Mario Gustavo. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Oficina de CoordinaciĂłn Administrativa Houssay. Instituto de FisiologĂa y BiofĂsica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de FisiologĂa y BiofĂsica Bernardo Houssay; ArgentinaFil: Grippo, M.. No especifĂca;Fil: VĂĄzquez, H.. No especifĂca;Fil: MorĂłs, C.. No especifĂca;Fil: Di Santo, M.. No especifĂca;Fil: Villa, A.. No especifĂca;Fil: Lazota, P.. No especifĂca;Fil: Foti, M.. No especifĂca;Fil: Napoli, N.. No especifĂca;Fil: Katsikas, M. M.. No especifĂca;Fil: Tonello, L.. No especifĂca;Fil: Peña, J.. No especifĂca;Fil: Etcheverry, M.. No especifĂca;Fil: Iglesias, D.. No especifĂca;Fil: Alcalde, A. L.. No especifĂca;Fil: Bruera, M.J.. No especifĂca;Fil: Bruzzo, V.. No especifĂca;Fil: Giordano, P.. No especifĂca;Fil: Pena Acero, F.. No especifĂca;Fil: Netri Pelandi, G.. No especifĂca;Fil: Pastaro, D.. No especifĂca;Fil: Bleiz, J.. No especifĂca;Fil: RodrĂguez, M. F.. No especifĂca;Fil: Laghezza, L.. No especifĂca;Fil: Molina, M. B.. No especifĂca;Fil: Patynok, N.. No especifĂca;Fil: Chatelain, M. S.. No especifĂca;Fil: Aguilar, M. J.. No especifĂca;Fil: Gamboa, J.. No especifĂca;Fil: Cervan, M.. No especifĂca;Fil: Ruggeri, A.. No especifĂca;Fil: Marinelli, I.. No especifĂca;Fil: Checcacci, E.. No especifĂca;Fil: Meregalli, C.. No especifĂca;Fil: Damksy Barbosa, J.. No especifĂca;Fil: Fernie, L.. No especifĂca;Fil: FernĂĄndez, M. J.. No especifĂca;Fil: Saenz Tejeira, M.M.. No especifĂca;Fil: Cereigido, C.. No especifĂca;Fil: Nunell, A.. No especifĂca;Fil: Villar, D.. No especifĂca;Fil: Mansilla, A. D.. No especifĂca;Fil: Darduin, M. D.. No especifĂca
Role of Calcitonin Gene-Related Peptide in Bone Repair after Cyclic Fatigue Loading
Calcitonin gene related peptide (CGRP) is a neuropeptide that is abundant in the sensory neurons which innervate bone. The effects of CGRP on isolated bone cells have been widely studied, and CGRP is currently considered to be an osteoanabolic peptide that has effects on both osteoclasts and osteoblasts. However, relatively little is known about the physiological role of CGRP in-vivo in the skeletal responses to bone loading, particularly fatigue loading.We used the rat ulna end-loading model to induce fatigue damage in the ulna unilaterally during cyclic loading. We postulated that CGRP would influence skeletal responses to cyclic fatigue loading. Rats were fatigue loaded and groups of rats were infused systemically with 0.9% saline, CGRP, or the receptor antagonist, CGRP(8-37), for a 10 day study period. Ten days after fatigue loading, bone and serum CGRP concentrations, serum tartrate-resistant acid phosphatase 5b (TRAP5b) concentrations, and fatigue-induced skeletal responses were quantified. We found that cyclic fatigue loading led to increased CGRP concentrations in both loaded and contralateral ulnae. Administration of CGRP(8-37) was associated with increased targeted remodeling in the fatigue-loaded ulna. Administration of CGRP or CGRP(8-37) both increased reparative bone formation over the study period. Plasma concentration of TRAP5b was not significantly influenced by either CGRP or CGRP(8-37) administration.CGRP signaling modulates targeted remodeling of microdamage and reparative new bone formation after bone fatigue, and may be part of a neuronal signaling pathway which has regulatory effects on load-induced repair responses within the skeleton
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