Studying inhibition of calcium oxalate stone formation: an in <italic>vitro</italic> approach for screening hydrogen sulfide and its metabolites

ABSTRACTPurpose:Calcium oxalate urolithiasis is one of the most common urinary tract diseases and is of high prevalence. The present study proposes to evaluate the antilithiatic property of hydrogen sulfide and its metabolites like thiosulfate & sulfate in an in vitro model.Materials and Methods:The antilithiatic activity of sodium hydrogen sulfide (NaSH), sodium thiosulfate (Na2S2O3) and sodium sulfate (Na2SO4) on the kinetics of calcium oxalate crystal formation was investigated both in physiological buffer and in urine from normal and recurrent stone forming volunteers. The stones were characterized by optical and spectroscopic techniques.Results:The stones were characterized to be monoclinic, prismatic and bipyramidal habit which is of calcium monohydrate and dihydrate nature. The FTIR displayed fingerprint corresponding to calcium oxalate in the control while in NaSH treated, S=O vibrations were visible in the spectrum. The order of percentage inhibition was NaSH>Na2S2O3>Na2SO4.Conclusion:Our study indicates that sodium hydrogen sulfide and its metabolite thiosulfate are inhibitors of calcium oxalate stone agglomeration which makes them unstable both in physiological buffer and in urine. This effect is attributed to pH changes and complexing of calcium by S2O32-and SO42- moiety produced by the test compounds.</sec

Food and feeding Biology of Fish Epinephelus malabaricus of Palk Bay and Gulf of Mannar coastal waters

The present study addresses this issue by examining the patterning of Cytochrome Oxidase I diversity in the stone fish Uranoscopus oligolepis the structurally diverse group of Family Uranoscopidae. The sequences were analyzed for their species identification using BOLD’s identification engine. The COI sequences of U. oligolepis from different geographical regions were extracted from NCBI for intra species variation analysis. All sequences were aligned using Clustal W. The sequences were trimmed using software and phylogenetic tree was constructed with bootstrap test. The results showed that the cytosine content was high (31%). The least molar concentration was observed in guanine (19.5%) and Adenine (19.6%). Thymine was the second predominant in molar concentration next to thymine which is followed by adenine. The G+C content was found to be 49.6% and A+T content was 50.4%. Leucine and Alanine content was high in the amino acid composition. From the study it is assumed that the mitochondrial gene COI can be the potential barcoding region to identify an organism up to the species level

Hydroponics agriculture as a modern agriculture technique

Purpose Hydroponics, also known as controlled environment agriculture, is a method of cultivating plants and herbs without soil using mineral-supplemented solutions. Agriculture biotechnology enhances its wings on developing genetically modified plants for expanding crop yield and introducing characteristic features such as growing seasonal crops throughout the year, weather-resistant, and pest resistance. Compared to traditional agriculture, hydroponic cultivation yields high-quality crops with 90% more efficient use of water. Therefore, hydroponic cultivation could be considered a succeeding future of agriculture. In this context, understanding agriculture through the hydroponic route is vital for the efficient cultivation of crops. This review elaborates on the different classes of hydroponic systems and the factors that enable the systematic elements of the frame-up. Design/methodology/approach The significant intent of this review is to provide information on distinct hydroponic systems. Findings The present review reports a comprehensive discussion about the significance of the hydroponics system, its mechanism, nutrient solution preparation, types of hydroponic setup, and the challenges faced and would light up the knowledge in the same. Originality/value This review focus on the current feasible hydroponic method of crop cultivation