131 research outputs found
ESR of MnO embedded in silica nanoporous matrices with different topologies
Electron spin resonance (ESR) experiments were performed with
antiferromagnetic MnO confined within a porous vycor-type glass and within
MCM-type channel matrices. A signal from confined MnO shows two components from
crystallized and amorphous MnO and depends on the pore topology. Crystallized
MnO within a porous glass shows a behavior having many similarities to the
bulk. In contrast with the bulk the strong ESR signal due to disordered
"surface" spins is observed below the magnetic transition. With the decrease of
channel diameter the fraction of amorphous MnO increases while the amount of
crystallized MnO decreases. The mutual influence of amorphous and crystalline
MnO is observed in the matrices with a larger channel diameter. In the matrices
with a smaller channel diameter the ESR signal mainly originates from amorphous
MnO and its behavior is typical for the highly disordered magnetic system.Comment: 7 pages pdf file, 5 figure
Magnetic versus nonmagnetic doping effects on the magnetic ordering in the Haldane chain compound PbNi2V2O8
A study of an impurity driven phase-transition into a magnetically ordered
state in the spin-liquid Haldane chain compound PbNi2V2O8 is presented. Both,
macroscopic magnetization as well as 51V nuclear magnetic resonance (NMR)
measurements reveal that the spin nature of dopants has a crucial role in
determining the stability of the induced long-range magnetic order. In the case
of nonmagnetic (Mg2+) doping on Ni2+ spin sites (S=1) a metamagnetic transition
is observed in relatively low magnetic fields. On the other hand, the magnetic
order in magnetically (Co2+) doped compounds survives at much higher magnetic
fields and temperatures, which is attributed to a significant anisotropic
impurity-host magnetic interaction. The NMR measurements confirm the predicted
staggered nature of impurity-liberated spin degrees of freedom, which are
responsible for the magnetic ordering. In addition, differences in the
broadening of the NMR spectra and the increase of nuclear spin-lattice
relaxation in doped samples, indicate a diverse nature of electron spin
correlations in magnetically and nonmagnetically doped samples, which begin
developing at rather high temperatures with respect to the antiferromagnetic
phase transition.Comment: 10 pages, 7 figure
An electron paramagnetic resonance study of Pr_{0.6}Ca_{0.4}MnO_{3} across the charge ordering transition
We report the first electron paramagnetic resonance studies of single
crystals and powders of Pr_{0.6}Ca_{0.4}MnO_{3} in the 300-4.2 K range,
covering the charge ordering transition at ~ 240 K and antiferromagnetic
transition (T_N) at ~ 170 K. The asymmetry parameter for the Dysonian single
crystal spectra shows anomalous increase at T_{co}. Below T_{co} the g-value
increases continuously, suggesting a gradual strengthening of orbital ordering.
The linewidth undergoes a sudden increase at T_{co} and continues to increase
down to T_N. The intensity increases as the temperature is decreased till
T_{co} due to the renormalization of magnetic susceptibility arising from the
build up of ferromagnetic correlations. The value of the exchange constant, J,
is estimated to be 154 K.Comment: Uses Revtex3.
Preliminary ancient DNA screening results from first to eighth century AD sites in Samtavro and Tchkantiskedi, Georgia
Ancient DNA allows the analysis of the genetic makeup of past populations. However, due to the highly variable nature of DNA preservation it is often useful to screen samples in order to assess the quality of DNA preservation before deciding how to invest resources into further sequencing efforts. Here we present screening results of 34 human bone and teeth samples showing a high variation in DNA preservation not only across the whole cemetery but also within tombs of the same cemetery. Preliminary analyses show that preservation levels are satisfactory for genetic characterisation of this ancient population.Luka Papac, Bastien Llamas, Alan Cooper, Varsha Pilbrow and Wolfgang Haa
Relations between lipoprotein(a) concentrations, LPA genetic variants, and the risk of mortality in patients with established coronary heart disease: a molecular and genetic association study
Background:
Lipoprotein(a) concentrations in plasma are associated with cardiovascular risk in the general population. Whether lipoprotein(a) concentrations or LPA genetic variants predict long-term mortality in patients with established coronary heart disease remains less clear.
Methods:
We obtained data from 3313 patients with established coronary heart disease in the Ludwigshafen Risk and Cardiovascular Health (LURIC) study. We tested associations of tertiles of lipoprotein(a) concentration in plasma and two LPA single-nucleotide polymorphisms ([SNPs] rs10455872 and rs3798220) with all-cause mortality and cardiovascular mortality by Cox regression analysis and with severity of disease by generalised linear modelling, with and without adjustment for age, sex, diabetes diagnosis, systolic blood pressure, BMI, smoking status, estimated glomerular filtration rate, LDL-cholesterol concentration, and use of lipid-lowering therapy. Results for plasma lipoprotein(a) concentrations were validated in five independent studies involving 10 195 patients with established coronary heart disease. Results for genetic associations were replicated through large-scale collaborative analysis in the GENIUS-CHD consortium, comprising 106 353 patients with established coronary heart disease and 19 332 deaths in 22 studies or cohorts.
Findings:
The median follow-up was 9·9 years. Increased severity of coronary heart disease was associated with lipoprotein(a) concentrations in plasma in the highest tertile (adjusted hazard radio [HR] 1·44, 95% CI 1·14–1·83) and the presence of either LPA SNP (1·88, 1·40–2·53). No associations were found in LURIC with all-cause mortality (highest tertile of lipoprotein(a) concentration in plasma 0·95, 0·81–1·11 and either LPA SNP 1·10, 0·92–1·31) or cardiovascular mortality (0·99, 0·81–1·2 and 1·13, 0·90–1·40, respectively) or in the validation studies.
Interpretation:
In patients with prevalent coronary heart disease, lipoprotein(a) concentrations and genetic variants showed no associations with mortality. We conclude that these variables are not useful risk factors to measure to predict progression to death after coronary heart disease is established.
Funding:
Seventh Framework Programme for Research and Technical Development (AtheroRemo and RiskyCAD), INTERREG IV Oberrhein Programme, Deutsche Nierenstiftung, Else-Kroener Fresenius Foundation, Deutsche Stiftung für Herzforschung, Deutsche Forschungsgemeinschaft, Saarland University, German Federal Ministry of Education and Research, Willy Robert Pitzer Foundation, and Waldburg-Zeil Clinics Isny
Ion conducting and paramagnetic d-PCL(530)/siloxane-based biohybrids doped with Mn 2+ ions
Amorphous α,ω-hidroxylpoly(ε-caprolactone) (PCL(530))/siloxane ormolytes doped
with manganese perchlorate (Mn(ClO4)2) (d-PCL(530)/siloxanenMn(ClO4)2) with n =
20, 50, and 100), thermally stable up to at least 200 ºC, were synthesized by the sol-gel
method. Ionic conductivity values up to 4.8×10−8 and 2.0×10−6 S cm−1 at about 25 and
100 ºC, respectively, where obtained for n = 20. FT-IR data demonstrated that the
hydrogen bonding interactions present in the non-doped d-PCL(530)/siloxane host
hybrid matrix were significantly influenced by the inclusion of Mn(ClO4)2 which
promoted the formation of more oxyethylene/urethane and urethane/urethane
aggregates. In addition, the Mn2+ ions bonded to all the “free” C=O groups of the
urethane cross-links and to some of the “free” ester groups of the amorphous PCL(530)
chains. In the electrolytes, the ClO4
− ions were found “free” and bonded to the Mn2+
ions along a bidentate configuration. The magnitude of the electron paramagnetic
resonance (EPR) hyperfine constant of the analyzed samples (A ≈ 90×10-4
cm−1
)
suggested that the bonding between Mn2+ ions and the surrounding ligands is
moderately ionic. The synthetized d-PCL(530)/siloxanenMn(ClO4)2 biohybrids have
potential application in paramagnetic, photoelectrochemical and electrochromic devices.This work was supported by Fundacao para a Ciencia e a Tecnologia (FCT) and Feder (contracts PTDC/CTM-BPC/112774/2009, PEst-OE/QUI/UI0616/2014 and PEst-C/QUI/UI0686/2013) and COST Action MP1202 "Rational design of hybrid organic-inorganic interfaces". R.F.P.P. acknowledges FCT for a grant (SFRH/BPD/87759/2012). M.M.S. acknowledges CNPq (PVE grant 406617/2013-9), for a mobility grant. The financial support of the Brazilian agencies Capes and CNPq are gratefully acknowledged. Research was partially financed by the CeRTEV, Center for Research, Technology and Education in Vitreous Materials, FAPESP 2013/07793-6.info:eu-repo/semantics/publishedVersio
RefGenes: identification of reliable and condition specific reference genes for RT-qPCR data normalization
Background
RT-qPCR is a sensitive and increasingly used method for gene expression quantification. To normalize RT-qPCR measurements between samples, most laboratories use endogenous reference genes as internal controls. There is increasing evidence, however, that the expression of commonly used reference genes can vary significantly in certain contexts.
Results
Using the Genevestigator database of normalized and well-annotated microarray experiments, we describe the expression stability characteristics of the transciptomes of several organisms. The results show that a) no genes are universally stable, b) most commonly used reference genes yield very high transcript abundances as compared to the entire transcriptome, and c) for each biological context a subset of stable genes exists that has smaller variance than commonly used reference genes or genes that were selected for their stability across all conditions.
Conclusion
We therefore propose the normalization of RT-qPCR data using reference genes that are specifically chosen for the conditions under study. RefGenes is a community tool developed for that purpose. Validation RT-qPCR experiments across several organisms showed that the candidates proposed by RefGenes generally outperformed commonly used reference genes. RefGenes is available within Genevestigator at http://www.genevestigator.com
Associations of Polymorphisms in the Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha Gene With Subsequent Coronary Heart Disease: An Individual-Level Meta-Analysis
Background: The knowledge of factors influencing disease progression in patients with established coronary heart disease (CHD) is still relatively limited. One potential pathway is related to peroxisome proliferator–activated receptor gamma coactivator-1 alpha (PPARGC1A), a transcription factor linked to energy metabolism which may play a role in the heart function. Thus, its associations with subsequent CHD events remain unclear. We aimed to investigate the effect of three different SNPs in the PPARGC1A gene on the risk of subsequent CHD in a population with established CHD.
Methods: We employed an individual-level meta-analysis using 23 studies from the GENetIcs of sUbSequent Coronary Heart Disease (GENIUS-CHD) consortium, which included participants (n = 80,900) with either acute coronary syndrome, stable CHD, or a mixture of both at baseline. Three variants in the PPARGC1A gene (rs8192678, G482S; rs7672915, intron 2; and rs3755863, T528T) were tested for their associations with subsequent events during the follow-up using a Cox proportional hazards model adjusted for age and sex. The primary outcome was subsequent CHD death or myocardial infarction (CHD death/myocardial infarction). Stratified analyses of the participant or study characteristics as well as additional analyses for secondary outcomes of specific cardiovascular disease diagnoses and all-cause death were also performed.
Results: Meta-analysis revealed no significant association between any of the three variants in the PPARGC1A gene and the primary outcome of CHD death/myocardial infarction among those with established CHD at baseline: rs8192678, hazard ratio (HR): 1.01, 95% confidence interval (CI) 0.98–1.05 and rs7672915, HR: 0.97, 95% CI 0.94–1.00; rs3755863, HR: 1.02, 95% CI 0.99–1.06. Similarly, no significant associations were observed for any of the secondary outcomes. The results from stratified analyses showed null results, except for significant inverse associations between rs7672915 (intron 2) and the primary outcome among 1) individuals aged ≥65, 2) individuals with renal impairment, and 3) antiplatelet users.
Conclusion: We found no clear associations between polymorphisms in the PPARGC1A gene and subsequent CHD events in patients with established CHD at baseline
An enigmatic hypoplastic defect of the maxillary lateral incisor in recent and fossil orangutans from Sumatra (Pongo abelii) and Borneo (Pongo pygmaeus)
Developmental dental pathologies provide insight into health of primates during ontogeny, and are particularly useful for elucidating the environment in which extant and extinct primates matured. Our aim is to evaluate whether the prevalence of an unusual dental defect on the mesiolabial enamel of the upper lateral incisor, thought to reflect dental crowding during maturation, is lesser in female orangutans, with their smaller teeth, than in males; and in Sumatran orangutans, from more optimal developmental habitats, than in those from Borneo. Our sample includes 49 Pongo pygmaeus (87 teeth), 21 P. abelii (38 teeth), Late Pleistocene paleo-orangutans from Sumatra and Vietnam (67 teeth), Late Miocene catarrhines Lufengpithecus lufengensis (2 teeth), and Anapithecus hernyaki (7 teeth). Methods include micro-CT scans, radiography, and dental metrics of anterior teeth. We observed fenestration between incisor crypts and marked crowding of unerupted crowns, which could allow tooth-to-tooth contact. Tooth size does not differ significantly in animals with or without the defect, implicating undergrowth of the jaw as the proximate cause of dental crowding and defect presence. Male orangutans from both islands show more defects than do females. The defect is significantly more common in Bornean orangutans (71 %) compared to Sumatran (29 %). Prevalence among fossil forms falls between these extremes, except that all five individual Anapithecus show one or both incisors with the defect. We conclude that maxillary lateral incisor defect is a common developmental pathology of apes that is minimized in optimal habitats and that such evidence can be used to infer habitat quality in extant and fossil apes
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