446 research outputs found
Vacancy defects in epitaxial thin film CuGaSe2 and CuInSe2
Epitaxial thin film CuGaSe2 and CuInSe2 samples grown on GaAs substrates with varying [Cu]/[Ga,In] ratios were studied using positron annihilation Doppler-broadening spectroscopy and were compared to bulk crystals. We find both Cu monovacancies and Cu-Se divacancies in CuInSe2, whereas, in CuGaSe2, the only observed vacancy defect is the Cu-Se divacancy.Peer reviewe
Local Bacteria Affect the Efficacy of Chemotherapeutic Drugs
In this study, the potential effects of bacteria on the efficacy of frequently used chemotherapies was examined. Bacteria and cancer cell lines were examined in vitro and in vivo for changes in the efficacy of cancer cell killing mediated by chemotherapeutic agents. Of 30 drugs examined in vitro, the efficacy of 10 was found to be significantly inhibited by certain bacteria, while the same bacteria improved the efficacy of six others. HPLC and mass spectrometry analyses of sample drugs (gemcitabine, fludarabine, cladribine, CB1954) demonstrated modification of drug chemical structure. The chemoresistance or increased cytotoxicity observed in vitro with sample drugs (gemcitabine and CB1954) was replicated in in vivo murine subcutaneous tumour models. These findings suggest that bacterial presence in the body due to systemic or local infection may influence tumour responses or off-target toxicity during chemotherapy
Towards integration of research and monitoring at forest ecosystems in Europe
Aim of study: The main aim of the work was to summarize availability, quality and comparability of on-going European Research and Monitoring Networks (ERMN), based on the results of a COST FP0903 Action questionnaire carried out in September 2010 and May 2012. Area of study: The COST Action FP0903 involves 29 European countries and 4 non-COST institutions from USA, Morocco and Tunisia. In this study, the total of 22 replies to the questionnaire from 18 countries were included. Materials and methods: Based on the feedback from the Action FP0903 countries, the most popular European Networks were identified. Thereafter, the access to the network database, available quality assurance/quality control procedures and publication were described. Finally, the so-called âSupersitesâ concept, defined as a âhighly instrumented research infrastructure, for both research and monitoring of soil-plant-atmosphere interactionsâ was discussed. Main results: The result of the survey indicate that the vast majority of the Action FP0903 countries participate in the International Cooperative Programme on Assessment and Monitoring of Air Pollution Effects on Forest (ICP Forest). The multi-disciplinary International Cooperative Programme on Integrated Monitoring of Air Pollution Effects on Ecosystems (ICPIM) is the second most widespread forest programme. Research highlights: To fully understand biochemical cycles in forest ecosystems, long-term monitoring is needed. Hence, a network of âSupersitesâ, is proposed. The application of the above infrastructure can be an effective way to attain a better integration of research and monitoring networks at forest sites in Europ
Three-dimensional visualization software assists learning in students with diverse spatial intelligence in medical education
This study evaluated effect of mental rotation (MR) training on learning outcomes and explored effectiveness of teaching via three-dimensional (3D) software among medical students with diverse spatial intelligence. Data from n = 67 student volunteers were included. A preliminary test was conducted to obtain baseline level of MR competency and was utilized to assign participants to two experimental conditions, i.e., trained group (n = 25) and untrained group (n = 42). Data on the effectiveness of training were collected to measure participants\u27 speed and accuracy in performing various MR activities. Six weeks later, a large class format (LCF) session was conducted for all students using 3D software. The usefulness of technology-assisted learning at the LCF was evaluated via a pre- and post-test. Students\u27 feedback regarding MR training and use of 3D software was acquired through questionnaires. MR scores of the trainees improved from 25.9±4.6 points to 28.1±4.4 (P = 0.011) while time taken to complete the tasks reduced from 20.9±3.9 to 12.2±4.4 minutes. Males scored higher than females in all components (P = 0.016). Further, higher pre- and post-test scores were observed in trained (9.0±1.9 and 12.3±1.6) versus untrained group (7.8±1.8; 10.8±1.8). Although mixed-design analysis of variance suggested significant difference in their test scores (P \u3c 0.001), both groups reported similar trend in improvement by means of 3D software (P = 0.54). Ninety-seven percent of students reported technology-assisted learning as an effective means of instruction and found use of 3D software superior to plastic models. Software based on 3D technologies could be adopted as an effective teaching pedagogy to support learning across students with diverse levels of mental rotation abilities
The Great Observatories Origins Deep Survey. VLT/FORS2 Spectroscopy in the GOODS-South Field: Part III
Aims. We present the full data set of the spectroscopic campaign of the
ESO/GOODS program in the GOODS-South field, obtained with the
FORS2 spectrograph at the ESO/VLT. Method. Objects were selected as
candidates for VLT/FORS2 observations primarily based on the expectation that
the detection and measurement of their spectral features would benefit from the
high throughput and spectral resolution of FORS2. The reliability of the
redshift estimates is assessed using the redshift-magnitude and color-redshift
diagrams, and comparing the results with public data. Results. Including the
third part of the spectroscopic campaign (12 masks) to the previous work (26
masks, Vanzella et al. 2005, 2006), 1715 spectra of 1225 individual targets
have been analyzed. The actual spectroscopic catalog provides 887 redshift
determinations. The typical redshift uncertainty is estimated to be sigma(z) ~
0.001. Galaxies have been selected adopting different color criteria and using
photometric redshifts. The resulting redshift distribution typically spans two
domains: from z=0.5 to 2 and z=3.5 to 6.3. The reduced spectra and the derived
redshifts are released to the community through the ESO web page
http://www.eso.org/science/goods/Comment: 11 pages, 11 figures; accepted for publication in Astronomy &
Astrophysics. Data are available at http://www.eso.org/science/goods
Enteric dysbiosis and fecal calprotectin expression in premature infants.
BackgroundPremature infants often develop enteric dysbiosis with a preponderance of Gammaproteobacteria, which has been related to adverse clinical outcomes. We investigated the relationship between increasing fecal Gammaproteobacteria and mucosal inflammation, measured by fecal calprotectin (FC).MethodsStool samples were collected from very-low-birth weight (VLBW) infants at â€2, 3, and 4 weeks' postnatal age. Fecal microbiome was surveyed using polymerase chain reaction amplification of the V4 region of 16S ribosomal RNA, and FC was measured by enzyme immunoassay.ResultsWe enrolled 45 VLBW infants (gestation 27.9â±â2.2 weeks, birth weight 1126â±â208âg) and obtained stool samples at 9.9â±â3, 20.7â±â4.1, and 29.4â±â4.9 days. FC was positively correlated with the genus Klebsiella (râ=â0.207, pâ=â0.034) and its dominant amplicon sequence variant (râ=â0.290, pâ=â0.003), but not with the relative abundance of total Gammaproteobacteria. Klebsiella colonized the gut in two distinct patterns: some infants started with low Klebsiella abundance and gained these bacteria over time, whereas others began with very high Klebsiella abundance.ConclusionIn premature infants, FC correlated with relative abundance of a specific pathobiont, Klebsiella, and not with that of the class Gammaproteobacteria. These findings indicate a need to define dysbiosis at genera or higher levels of resolution
Galaxy Counterparts of metal-rich Damped Lyman-alpha Absorbers - I: The case of the z=2.35 DLA towards Q2222-0946
We have initiated a survey using the newly commissioned X-shooter
spectrograph to target candidate relatively metal-rich damped Lyman-alpha
absorbers (DLAs). The spectral coverage of X-shooter allows us to search for
not only Lyman-alpha emission, but also rest-frame optical emission lines. We
have chosen DLAs where the strongest rest-frame optical lines ([OII], [OIII],
Hbeta and Halpha) fall in the NIR atmospheric transmission bands. In this first
paper resulting from the survey, we report on the discovery of the galaxy
counterpart of the z_abs = 2.354 DLA towards the z=2.926 quasar Q2222$-0946.
This DLA is amongst the most metal-rich z>2 DLAs studied so far at comparable
redshifts and there is evidence for substantial depletion of refractory
elements onto dust grains. We measure metallicities from ZnII, SiII, NiII, MnII
and FeII of -0.46+/-0.07, -0.51+/-0.06, -0.85+/-0.06, -1.23+/-0.06, and
-0.99+/-0.06, respectively. The galaxy is detected in the Lyman-alpha, [OIII]
lambda4959,5007 Halpha emission lines at an impact parameter of about 0.8
arcsec (6 kpc at z_abs = 2.354). We infer a star-formation rate of 10 M_sun
yr^-1, which is a lower limit due to the possibility of slit-loss. Compared to
the recently determined Halpha luminosity function for z=2.2 galaxies the
DLA-galaxy counterpart has a luminosity of L~0.1L^*_Halpha. The emission-line
ratios are 4.0 (Lyalpha/Halpha) and 1.2 ([OIII]/Halpha). The Lyalpha line shows
clear evidence for resonant scattering effects, namely an asymmetric,
redshifted (relative to the systemic redshift) component and a much weaker
blueshifted component. The fact that the blueshifted component is relatively
weak indicates the presence of a galactic wind. The properties of the galaxy
counterpart of this DLA is consistent with the prediction that metal-rich DLAs
are associated with the most luminous of the DLA-galaxy counterparts.Comment: 9 pages, 7 figures. Accepted for publication in MNRA
High-confidence glycosome proteome for procyclic form <em>Trypanosoma brucei</em> by epitope-tag organelle enrichment and SILAC proteomics
The glycosome of the pathogenic African trypanosome Trypanosoma brucei is a specialized peroxisome that contains most of the enzymes of glycolysis and several other metabolic and catabolic pathways. The contents and transporters of this membrane-bounded organelle are of considerable interest as potential drug targets. Here we use epitope tagging, magnetic bead enrichment, and SILAC quantitative proteomics to determine a high-confidence glycosome proteome for the procyclic life cycle stage of the parasite using isotope ratios to discriminate glycosomal from mitochondrial and other contaminating proteins. The data confirm the presence of several previously demonstrated and suggested pathways in the organelle and identify previously unanticipated activities, such as protein phosphatases. The implications of the findings are discussed
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