Airborne multi-axis DOAS measurements of atmospheric trace gases on CARIBIC long-distance flights

A DOAS (<i>Differential Optical Absorption Spectroscopy</i>) instrument was implemented and operated onboard a long-distance passenger aircraft within the framework of the CARIBIC project (<i>Civil Aircraft for the Regular Investigation of the atmosphere Based on an Instrument Container</i>). The instrument was designed to keep weight, size and power consumption low and to comply with civil aviation regulations. It records spectra of scattered light from three viewing directions (nadir, 10° above and below horizon) using a miniaturized telescope system. The telescopes are integrated in the main pylon of the inlet system which is mounted at the belly of the aircraft. Fibre bundles transmit light from the telescopes to spectrograph-detector units inside the DOAS container instrument. The latter is part of the removable CARIBIC instrument container, which is installed monthly on the aircraft for a series of measurement flights. <br><br> During 30 flight operations within three years, measurements of HCHO, HONO, NO<sub>2</sub>, BrO, O<sub>3</sub> and the oxygen dimer O<sub>4</sub> were conducted. All of these trace gases except BrO could be analysed with a 30 s time resolution. HONO was detected for the first time in a deep convective cloud over central Asia, while BrO, NO<sub>2</sub> and O<sub>3</sub> could be observed in tropopause fold regions. Biomass burning signatures over South America could be seen and measurements during ascent and descent provided information on boundary layer trace gas profiles (e.g. NO<sub>2</sub> or HCHO)

Systematic combination of Lean Management with digitalization to improve production systems on the example of Jidoka 4.0

© The Author(s) 2020. Lean Management builds the basis for efficient production systems for many industrial companies. However, lots of potentials of Lean Management have been lifted and information and communication technologies in the context of digitalization and cyber-physical production systems (CPPS) offer new possibilities to enhance the performance of companies. Even though surveys indicate that companies recognize these potentials, especially small and medium-sized companies still face challenges in selection and implementation of suitable solutions. Thus, the research project GaProSys 4.0 aims at supporting companies with a systematic approach to combine existing structures of Lean Management with potentials of digitalization in development of a new set of methods to enhance production systems. This paper presents the approach of the research project to develop a structured set of methods and provides an example to illustrate the potentials

CMTr mediated 2`-O-ribose methylation status of cap adjacent-nucleotides across animals

ACKNOWLEDGMENTS We thank Mark Carrington and Nancy Standard for T. brucei total RNA, Jane Nimmo for honeybees, Caroline Chadwick for mouse tissue, Pawel Grzechnik for HEK293T cells, Roland Arnold for HCT116 cells, Rupert Fray for plasmids, and the National BioResource Project, Tokyo, Japan for the C. elegans CMTr2 strain. M.S. acknowledges funding from the Leverhulme Trust and the Biotechnology and Biological Sciences Research Council (BBSRC) (BB/R002932/1), J.P. and B.M. from BBSRC (BB/T002859/1), and F.M. from the Wellcome Trust (106955/Z/15/Z).Peer reviewedPublisher PD

The DLV System for Knowledge Representation and Reasoning

This paper presents the DLV system, which is widely considered the state-of-the-art implementation of disjunctive logic programming, and addresses several aspects. As for problem solving, we provide a formal definition of its kernel language, function-free disjunctive logic programs (also known as disjunctive datalog), extended by weak constraints, which are a powerful tool to express optimization problems. We then illustrate the usage of DLV as a tool for knowledge representation and reasoning, describing a new declarative programming methodology which allows one to encode complex problems (up to $\Delta^P_3$-complete problems) in a declarative fashion. On the foundational side, we provide a detailed analysis of the computational complexity of the language of DLV, and by deriving new complexity results we chart a complete picture of the complexity of this language and important fragments thereof. Furthermore, we illustrate the general architecture of the DLV system which has been influenced by these results. As for applications, we overview application front-ends which have been developed on top of DLV to solve specific knowledge representation tasks, and we briefly describe the main international projects investigating the potential of the system for industrial exploitation. Finally, we report about thorough experimentation and benchmarking, which has been carried out to assess the efficiency of the system. The experimental results confirm the solidity of DLV and highlight its potential for emerging application areas like knowledge management and information integration.Comment: 56 pages, 9 figures, 6 table

Evaluation of electrical signals in pine trees in a mediterranean forest ecosystem

This is an Accepted Manuscript of an article published by Taylor & Francis in Plant Signaling and Behaviour on 2020, available online: http://www.tandfonline.com/10.1080/15592324.2020.1795580[EN] Electric potential differences in living plants are explained by theories based on sap flow. In order to acquire more advanced knowledge about the spatial distribution of these electric potential measures in trees, this research aims to analyze electrical signals in a population of Aleppo pines (Pinus halepensisMill.) in a representative Mediterranean forest ecosystem. The specific research objective is to assess some of the most significant factors that influence the distribution pattern of those electric signals: tree age, measurement type and electrode placement. The research has been conducted in representative forest stands, obtaining measurements of different representative trees. After a statistical evaluation of the obtained results, the main conclusions of our research are: A.Tree maturity influences directly on electric potential. B.Maximum electrical signals can be measured in young pines showing values of 0.6 V and 0.6 mu A for voltage and current, respectively. C.The distribution patterns of both voltage and short-circuit current depending on electrode placement are uniform.Zapata, R.; Oliver Villanueva, JV.; Lemus Zúñiga, LG.; Luzuriaga, JE.; Mateo Pla, MÁ.; Urchueguía Schölzel, JF. (2020). Evaluation of electrical signals in pine trees in a mediterranean forest ecosystem. Plant Signaling and Behaviour (Online). 15(10):1-9. https://doi.org/10.1080/15592324.2020.1795580S191510I. Further experiments on the more important physiological changes induced in the human economy by change of climate. (1873). Proceedings of the Royal Society of London, 21(139-147), 1-10. doi:10.1098/rspl.1872.0002Darwin, C. (1875). 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Electrical signaling, stomatal conductance, ABA and Ethylene content in avocado trees in response to root hypoxia. Plant Signaling & Behavior, 4(2), 100-108. doi:10.4161/psb.4.2.7872Ríos-Rojas, L., Morales-Moraga, D., Alcalde, J. A., & Gurovich, L. A. (2015). Use of plant woody species electrical potential for irrigation scheduling. Plant Signaling & Behavior, 10(2), e976487. doi:10.4161/15592324.2014.976487Cardoso SS, Carrondo LB, Marques JM, Narciso PN, Rocha MJ, Rodrigues IN, Soares A. (2004). Monitorization of the electrical signal generated by a tree. February 2004 – 4th luso-spanish assembly on geodesy and geophysics.Le Mouël, J.-L., Gibert, D., & Poirier, J.-P. (2010). On transient electric potential variations in a standing tree and atmospheric electricity. Comptes Rendus Geoscience, 342(2), 95-99. doi:10.1016/j.crte.2009.12.001Koppan A (2004). Variations of the natural electric potential differences occurring on tree trunks and their relationship with the xylem sap flow. 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The future of enterprise groupware applications

This paper provides a review of groupware technology and products. The purpose of this review is to investigate the appropriateness of current groupware technology as the basis for future enterprise systems and evaluate its role in realising, the currently emerging, Virtual Enterprise model for business organisation. It also identifies in which way current technological phenomena will transform groupware technology and will drive the development of the enterprise systems of the future

Memory consolidation in honey bees is enhanced by down-regulation of Down syndrome cell adhesion molecule and changes its alternative splicing

Down syndrome cell adhesion molecule (Dscam) gene encodes a cell adhesion molecule required for neuronal wiring. A remarkable feature of arthropod Dscam is massive alternative splicing generating thousands of different isoforms from three variable clusters of alternative exons. Dscam expression and diversity arising from alternative splicing have been studied during development, but whether they exert functions in adult brains has not been determined. Here, using honey bees, we find that Dscam expression is critically linked to memory retention as reducing expression by RNAi enhances memory after reward learning in adult worker honey bees. Moreover, alternative splicing of Dscam is altered in all three variable clusters after learning. Since identical Dscam isoforms engage in homophilic interactions, these results suggest a mechanism to alter inclusion of variable exons during memory consolidation to modify neuronal connections for memory retention

Civil Aircraft for the regular investigation of the atmosphere based on an instrumented container: The new CARIBIC system

An airfreight container with automated instruments for measurement of atmospheric gases and trace compounds was operated on a monthly basis onboard a Boeing 767-300 ER of LTU International Airways during long-distance flights from 1997 to 2002 (CARIBIC, Civil Aircraft for Regular Investigation of the Atmosphere Based on an Instrument Container, http://www.caribic-atmospheric.com). Subsequently a more advanced system has been developed, using a larger capacity container with additional equipment and an improved inlet system. CARIBIC phase #2 was implemented on a new long-range aircraft type Airbus A340-600 of the Lufthansa German Airlines (Star Alliance) in December 2004, creating a powerful flying observatory. The instrument package comprises detectors for the measurement of O3, total and gaseous H2O, NO and NOy, CO, CO2, O2, Hg, and number concentrations of sub-micrometer particles (>4 nm, >12 nm, and >18 nm diameter). Furthermore, an optical particle counter (OPC) and a proton transfer mass spectrometer (PTR-MS) are incorporated. Aerosol samples are collected for analysis of elemental composition and particle morphology after flight. Air samples are taken in glass containers for laboratory analyses of hydrocarbons, halocarbons and greenhouse gases (including isotopic composition of CO2) in several laboratories. Absorption tubes collect oxygenated volatile organic compounds. Three differential optical absorption spectrometers (DOAS) with their telescopes mounted in the inlet system measure atmospheric trace gases such as BrO, HONO, and NO2. A video camera mounted in the inlet provides information about clouds along the flight track. The flying observatory, its equipment and examples of measurement results are reported

A new multicompartmental reaction-diffusion modeling method links transient membrane attachment of E. coli MinE to E-ring formation

Many important cellular processes are regulated by reaction-diffusion (RD) of molecules that takes place both in the cytoplasm and on the membrane. To model and analyze such multicompartmental processes, we developed a lattice-based Monte Carlo method, Spatiocyte that supports RD in volume and surface compartments at single molecule resolution. Stochasticity in RD and the excluded volume effect brought by intracellular molecular crowding, both of which can significantly affect RD and thus, cellular processes, are also supported. We verified the method by comparing simulation results of diffusion, irreversible and reversible reactions with the predicted analytical and best available numerical solutions. Moreover, to directly compare the localization patterns of molecules in fluorescence microscopy images with simulation, we devised a visualization method that mimics the microphotography process by showing the trajectory of simulated molecules averaged according to the camera exposure time. In the rod-shaped bacterium _Escherichia coli_, the division site is suppressed at the cell poles by periodic pole-to-pole oscillations of the Min proteins (MinC, MinD and MinE) arising from carefully orchestrated RD in both cytoplasm and membrane compartments. Using Spatiocyte we could model and reproduce the _in vivo_ MinDE localization dynamics by accounting for the established properties of MinE. Our results suggest that the MinE ring, which is essential in preventing polar septation, is largely composed of MinE that is transiently attached to the membrane independently after recruited by MinD. Overall, Spatiocyte allows simulation and visualization of complex spatial and reaction-diffusion mediated cellular processes in volumes and surfaces. As we showed, it can potentially provide mechanistic insights otherwise difficult to obtain experimentally

Expression of high p53 levels in colorectal cancer: a favourable prognostic factor

The expression of p53 protein was examined in a series of 111 colorectal cancer adenocarcinomas with a long follow-up. A quantitative luminometric immunoassay (LIA) was used for the measurement of wild-type and mutant p53 protein in extracts from colorectal tumour cytosols, p53 being detected in 42% of the samples (range 0.0–52 ng mg−1). Using an arbitrary cut-off value of 2.7 ng mg−1, 25% of the tumours were classified as manifesting high p53 levels. There was no association of p53 expression with patient age, sex, serum preoperative carcinoembryonic antigen (CEA) levels, tumour site and size, nodal status or TNM stage. Significant and independent correlation was found to exist between high p53 levels and prolonged disease-free survival (P = 0.05) at a median follow-up of 60 months. This survival advantage was most apparent among stage III cancer patients. The results from this study would suggest that expression of high p53 levels appear to be useful in selecting a group of colorectal cancer patients with a better prognosis. © 1999 Cancer Research Campaig