Combined single-molecule force and fluorescence measurements for biology

Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes can now be studied with nanometer precision and millisecond time resolution

UCDs in the Coma Cluster

As part of the HST/ACS Coma Cluster Treasury Survey, we have undertaken a Keck/LRIS spectroscopic campaign to determine membership for faint dwarf galaxies. In the process, we discovered a population of Ultra Compact Dwarf galaxies (UCDs) in the core region of the Coma cluster. At the distance of Coma, UCDs are expected to have angular sizes 0.01 < R_e < 0.2 arcsec. With ACS imaging, we can resolve all but the smallest ones with careful fitting. Candidate UCDs were chosen based on magnitude, color, and degree of resolution. We spectroscopically confirm 27 objects as bona fide UCD members of the Coma cluster, a 60% success rate for objects targeted with M_R < -12. We attribute the high success rate in part to the high resolution of HST data and to an apparent large population of UCDs in Coma. We find that the UCDs tend to be strongly clustered around giant galaxies, at least in the core region of the cluster, and have a distribution and colors that are similar to globular clusters. These findings suggest that UCDs are not independent galaxies, but rather have a star cluster origin. This current study provides the dense environment datapoint necessary for understanding the UCD population.Comment: 6 pages, 9 figures, to appear in the conference proceedings of "A Universe of Dwarf Galaxies" (Lyon, June 14-18, 2010

Chemical evolution of the intra-cluster medium

The high metallicity of the intra-cluster medium (ICM) is generally interpreted on the base of the galactic wind scenario for elliptical galaxies. In this framework, we develop a toy-model to follow the chemical evolution of the ICM, formulated in analogy to chemical models for individual galaxies. The model computes the galaxy formation history (GFH) of cluster galaxies, connecting the final luminosity function (LF) to the corresponding metal enrichment history of the ICM. The observed LF can be reproduced with a smooth, Madau-plot like GFH peaking at z~ 1-2, plus a "burst" of formation of dwarf galaxies at high redshift. The model is used to test the response of the predicted metal content and abundance evolution of the ICM to varying input galactic models. The chemical enrichment is computed from "galactic yields" based on models of elliptical galaxies with a variable initial mass function (IMF), favouring the formation of massive stars at high redshift and/or in more massive galaxies. For a given final galactic luminosity, these model ellipticals eject into the ICM a larger quantity of gas and of metals than do standard models based on the Salpeter IMF. However, a scenario in which the IMF varies with redshift as a consequence of the effect of the the cosmic background temperature on the Jeans mass scale, appears to be too mild to account for the observed metal production in clusters. The high iron-mass-to-luminosity-ratio of the ICM can be reproduced only by assuming a more dramatic variation of the typical stellar mass, in line with other recent findings. The mass in the wind-ejected gas is predicted to exceed the mass in galaxies by a factor of 1.5-2 and to constitute roughly half of the intra-cluster gas.Comment: 25 pages, 17 figures, accepted for publication in A&

What determines the fraction of elliptical galaxies in clusters?

We study the correlation between the morphological mix of cluster galaxies and the assembly history of the parent cluster by taking advantage of two independently developed semi-analytic models for galaxy formation and evolution. In our models, both the number of cluster members and that of elliptical members increase as a function of cluster mass, in such a way that the resulting elliptical fractions are approximately independent of cluster mass. The population of cluster ellipticals exhibit a marked bimodal distribution as a function of galaxy stellar mass, with a dip at masses $\sim 10^{10}\,{\rm M}_{\odot}$. In the framework of our models, this bimodality originates from the combination of a strongly decreasing number of galaxies with increasing stellar mass, and a correspondingly increasing probability of experiencing major mergers. We show that the correlation between the measured elliptical fraction and the assembly history of the parent cluster is weak, and that it becomes stronger in models that adopt longer galaxy merger times. We argue that this results from the combined effect of a decreasing bulge production due to a reduced number of mergers, and an increasing survival probability of pre-existing ellipticals, with the latter process being more important than the former.Comment: 8 pages, 3 figures, accepted for publication in MNRA

Keck/LRIS Spectroscopic Confirmation of Coma Cluster Dwarf Galaxy Membership Assignments

Keck/LRIS multi-object spectroscopy has been carried out on 140 of some of the lowest and highest surface brightness faint (19 < R < 22) dwarf galaxy candidates in the core region of the Coma Cluster. These spectra are used to measure redshifts and establish membership for these faint dwarf populations. The primary goal of the low surface brightness sample is to test our ability to use morphological and surface brightness criteria to distinguish between Coma Cluster members and background galaxies using high resolution HST/ACS images. Candidates were rated as expected members, uncertain, or expected background. From 93 spectra, 51 dwarf galaxy members and 20 background galaxies are identified. Our morphological membership estimation success rate is ~100% for objects expected to be members and better than ~90% for galaxies expected to be in the background. We confirm that low surface brightness is a very good indicator of cluster membership. High surface brightness galaxies are almost always background with confusion arising only from the cases of the rare compact elliptical galaxies. The more problematic cases occur at intermediate surface brightness. Many of these galaxies are given uncertain membership ratings, and these were found to be members about half of the time. Including color information will improve membership determination but will fail for some of the same objects that are already mis-identified when using only surface brightness and morphology criteria. Compact elliptical galaxies with B-V colors ~0.2 magnitudes redward of the red sequence in particular require spectroscopic follow-up. In a sample of 47 high surface brightness, UCD candidates, 19 objects have redshifts which place them in the Coma Cluster. Redshift measurements are presented and the use of indirect means for establishing cluster membership is discussed.Comment: Accepted for publication in ApJ, 25 pages, 15 figure

The near-infrared luminosity function of cluster galaxies beyond redshift one

We determined the K band luminosity function (LF) of cluster galaxies at redshift z~1.2, using near-infrared images of three X-ray luminous clusters at z=1.11,1.24,1.27. The composite LF was derived down to M*+4, by means of statistical background subtraction, and is well described by a Schechter function with K*=20.5 AB mag and alpha=-1. From the K band composite LF we derived the stellar mass function of cluster galaxies. Using available X-ray mass profiles we determined the M/L ratios of these three clusters, which tend to be lower than those measured in the local universe. With these data, no significant difference can be seen between the shapes of the cluster galaxies LF and the LF of field galaxies at similar redshift. We also found no significant evolution out to z ~1.2 in the bright (<M*+4) part of the cluster galaxies LF probed in this study, apart from a brightening of ~1.3 mag of the characteristic magnitude of the high redshift LF. We confirm, and extend to higher redshift, the result from previous work that the redshift evolution of the characteristic magnitude M* is consistent with passive evolution of a stellar population formed at z>2. The results obtained in this work support and extend previous findings that most of the stars in bright galaxies were formed at high redshift, and that K-bright (M>10^11 Msun) galaxies were already in place at z ~ 1.2, at least in the central regions of X-ray luminous clusters. Together with recent results on the field galaxies stellar mass function, this implies that most of the stellar mass is already assembled in massive galaxies by z ~ 1, both in low and high density environments.Comment: 16 pages, 10 figures, to appear in A&

The HST/ACS Coma Cluster Survey. II. Data Description and Source Catalogs

The Coma cluster was the target of a HST-ACS Treasury program designed for deep imaging in the F475W and F814W passbands. Although our survey was interrupted by the ACS instrument failure in 2007, the partially completed survey still covers ~50% of the core high-density region in Coma. Observations were performed for 25 fields that extend over a wide range of cluster-centric radii (~1.75 Mpc) with a total coverage area of 274 arcmin^2. The majority of the fields are located near the core region of Coma (19/25 pointings) with six additional fields in the south-west region of the cluster. In this paper we present reprocessed images and SExtractor source catalogs for our survey fields, including a detailed description of the methodology used for object detection and photometry, the subtraction of bright galaxies to measure faint underlying objects, and the use of simulations to assess the photometric accuracy and completeness of our catalogs. We also use simulations to perform aperture corrections for the SExtractor Kron magnitudes based only on the measured source flux and half-light radius. We have performed photometry for ~73,000 unique objects; one-half of our detections are brighter than the 10-sigma point-source detection limit at F814W=25.8 mag (AB). The slight majority of objects (60%) are unresolved or only marginally resolved by ACS. We estimate that Coma members are 5-10% of all source detections, which consist of a large population of unresolved objects (primarily GCs but also UCDs) and a wide variety of extended galaxies from a cD galaxy to dwarf LSB galaxies. The red sequence of Coma member galaxies has a constant slope and dispersion across 9 magnitudes (-21<M_F814W<-13). The initial data release for the HST-ACS Coma Treasury program was made available to the public in 2008 August. The images and catalogs described in this study relate to our second data release.Comment: Accepted for publication in ApJS. A high-resolution version is available at http://archdev.stsci.edu/pub/hlsp/coma/release2/PaperII.pd

Presentation of arthritogenic peptide to antigen-specific T cells by fibroblast-like synoviocytes

Objective To assess the ability of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) to function as antigen-presenting cells (APCs) for arthritogenic autoantigens found within inflamed joint tissues. Methods Human class II major histocompatibility complex (MHC)–typed FLS were used as APCs for murine class II MHC–restricted CD4 T cell hybridomas. Interferon-Γ (IFNΓ)–treated, antigen-loaded FLS were cocultured with T cell hybridomas specific for immunodominant portions of human cartilage gp-39 (HC gp-39) or human type II collagen (CII). T cell hybridoma activation was measured by enzyme-linked immunosorbent assay of culture supernatants for interleukin-2. Both synthetic peptide and synovial fluid (SF) were used as sources of antigen. APC function in cocultures was inhibited by using blocking antibodies to human class II MHC, CD54, or CD58, or to murine CD4, CD11a, or CD2. Results Human FLS could present peptides from the autoantigens HC gp-39 and human CII to antigen-specific MHC-restricted T cell hybridomas. This response required pretreatment of FLS with IFNΓ, showed MHC restriction, and was dependent on human class II MHC and murine CD4 for effective antigen presentation. Furthermore, FLS were able to extract and present antigens found within human SF to both the HC gp-39 and human CII T cell hybridomas in an IFNΓ-dependent and MHC-restricted manner. Conclusion RA FLS can function as APCs and are able to present peptides derived from autoantigens found within joint tissues to activated T cells in vitro. In the context of inflamed synovial tissues, FLS may be an important and hitherto overlooked subset of APCs that could contribute to autoreactive immune responses.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/56038/1/22573_ftp.pd

Selective Expression of a Stable Cell Surface Molecule on Type 2 but Not Type 1 Helper T Cells

T helper cell type 1 (Th1) and 2 (Th2) are central to immune regulation. However, no stable cell surface marker capable of distinguishing and separating these two subsets of CD4+ cells has yet been found. Using differential display PCR, we have identified a gene encoding a cell membrane bound molecule, originally designated ST2L, T1, DER4, or Fit, expressed constitutively and stably on the surface of murine Th2s, but not Th1s even after stimulation with a range of immunological stimuli. Antibody against a peptide derived from ST2L strongly and stably labeled the surface of cloned Th2s but not Th1s, and Th2s but not Th1s derived from naive T cells of ovalbumin T cell receptor–α/β transgenic mice. Three-color single cell flow cytometric analysis shows that cell surface ST2L coexpressed with intracellular interleukin (IL)-4, but not with interferon (IFN)-γ. The antibody selectively lysed Th2s in vitro in a complement-dependent manner. In vivo, it enhanced Th1 responses by increasing IFN-γ production and decreasing IL-4 and IL-5 synthesis. It induced resistance to Leishmania major infection in BALB/c mice and exacerbated collagen-induced arthritis in DBA/1 mice. Thus, ST2L is a stable marker distinguishing Th2s from Th1s and is also associated with Th2 functions. Hence, it may be a target for therapeutic intervention

The HST/ACS Coma Cluster Survey. II. Data Description and Source Catalogs

The Coma cluster, Abell 1656, was the target of a HST-ACS Treasury program designed for deep imaging in the F475W and F814W passbands. Although our survey was interrupted by the ACS instrument failure in early 2007, the partially-completed survey still covers approximately 50% of the core high density region in Coma. Observations were performed for twenty-five fields with a total coverage area of 274 aremin(sup 2), and extend over a wide range of cluster-centric radii (approximately 1.75 Mpe or 1 deg). The majority of the fields are located near the core region of Coma (19/25 pointings) with six additional fields in the south-west region of the cluster. In this paper we present SEXTRACTOR source catalogs generated from the processed images, including a detailed description of the methodology used for object detection and photometry, the subtraction of bright galaxies to measure faint underlying objects, and the use of simulations to assess the photometric accuracy and completeness of our catalogs. We also use simulations to perform aperture corrections for the SEXTRACTOR Kron magnitudes based only on the measured source flux and its half-light radius. We have performed photometry for 76,000 objects that consist of roughly equal numbers of extended galaxies and unresolved objects. Approximately two-thirds of all detections are brighter than F814W=26.5 mag (AB), which corresponds to the 10sigma, point-source detection limit. We estimate that Coma members are 5-10% of the source detections, including a large population of compact objects (primarily GCs, but also cEs and UCDs), and a wide variety of extended galaxies from cD galaxies to dwarf low surface brightness galaxies. The initial data release for the HST-ACS Coma Treasury program was made available to the public in August 2008. The images and catalogs described in this study relate to our second data release