The gut microbiota, bile acids and their correlation in primary sclerosing cholangitis associated with inflammatory bowel disease.

BACKGROUND: Patients with primary sclerosing cholangitis associated with inflammatory bowel disease (PSC-IBD) have a very high risk of developing colorectal neoplasia. Alterations in the gut microbiota and/or gut bile acids could account for the increase in this risk. However, no studies have yet investigated the net result of cholestasis and a potentially altered bile acid pool interacting with a dysbiotic gut flora in the inflamed colon of PSC-IBD. AIM: The aim of this study was to compare the gut microbiota and stool bile acid profiles, as well as and their correlation in patients with PSC-IBD and inflammatory bowel disease alone. METHODS: Thirty patients with extensive colitis (15 with concomitant primary sclerosing cholangitis) were prospectively recruited and fresh stool samples were collected. The microbiota composition in stool was profiled using bacterial 16S rRNA sequencing. Stool bile acids were assessed by high-performance liquid chromatography tandem mass spectrometry. RESULTS: The total stool bile acid pool was significantly reduced in PSC-IBD. Although no major differences were observed in the individual bile acid species in stool, their overall combination allowed a good separation between PSC-IBD and inflammatory bowel disease. Compared with inflammatory bowel disease alone, PSC-IBD patients demonstrated a different gut microbiota composition with enrichment in Ruminococcus and Fusobacterium genus compared with inflammatory bowel disease. At the operational taxonomic unit level major shifts were observed within the Firmicutes (73%) and Bacteroidetes phyla (17%). Specific microbiota-bile acid correlations were observed in PSC-IBD, where 12% of the operational taxonomic units strongly correlated with stool bile acids, compared with only 0.4% in non-PSC-IBD. CONCLUSIONS: Patients with PSC-IBD had distinct microbiota and microbiota-stool bile acid correlations as compared with inflammatory bowel disease. Whether these changes are associated with, or may predispose to, an increased risk of colorectal neoplasia needs to be further clarified.info:eu-repo/semantics/publishedVersio

Resource Provisioning Exploiting Cost and Performance Diversity within IaaS Cloud Providers

IaaS platforms such as Amazon EC2 allow clients access to massive computational power in the form of instances. Amazon hosts three different instance purchasing options, each with its own SLA covering pricing and availability. Amazon also offers access to a number of geographical regions, zones, and instance types to select from. In this thesis, the problem of utilizing Spot and On-Demand instances is analyzed and two approaches are presented in order to exploit the cost and performance diversity among different instance types and availability zones, and among the Spot markets they represent. We first develop RAMP, a framework designed to calculate the expected profit of using a specific Spot or On-Demand instance through an evaluation of instance reliability. RAMP is extended to develop RAMC-DC, a framework designed to allocate the most cost effective instance through strategies that facilitate interchangeability of instances among short jobs, reliability of instances among long jobs, and a comparison of the estimated costs of possible allocations. RAMC-DC achieves fault tolerance through comparisons of the price dynamics across instance types and availability zones, and through an examination of three basic checkpointing methods. Evaluations demonstrate that both frameworks take a large step toward low-volatility, high cost-efficiency resource provisioning. While achieving early-termination rates as low as 2.2%, RAMP can completely offset the total cost when charging the user just 17.5% of the On-Demand price. Moreover, the increases in profit resulting from relatively small additional charges to users are notably high, i.e., 100% profit compared to the resource provisioning cost with 35% of the equivalent On-Demand price. RAMC-DC can maintain deadline breaches below 1.8% of all jobs, achieve both early-termination and deadline breach rates as low as 0.5% of all jobs, and lowers total costs by between 80% and 87% compared to using only On-Demand instances

Artelogie N°13 : Entretiens avec Patricia Díaz-Inostroza et Rodrigo Torres Alvarado.

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Gamma-ray signatures of cosmic ray acceleration, propagation, and confinement in the era of CTA

Galactic cosmic rays are commonly believed to be accelerated at supernova remnants via diffusive shock acceleration. Despite the popularity of this idea, a conclusive proof for its validity is still missing. Gamma-ray astronomy provides us with a powerful tool to tackle this problem, because gamma rays are produced during cosmic ray interactions with the ambient gas. The detection of gamma rays from several supernova remnants is encouraging, but still does not constitute a proof of the scenario, the main problem being the difficulty in disentangling the hadronic and leptonic contributions to the emission. Once released by their sources, cosmic rays diffuse in the interstellar medium, and finally escape from the Galaxy. The diffuse gamma-ray emission from the Galactic disk, as well as the gamma-ray emission detected from a few galaxies is largely due to the interactions of cosmic rays in the interstellar medium. On much larger scales, cosmic rays are also expected to permeate the intracluster medium, since they can be confined and accumulated within clusters of galaxies for cosmological times. Thus, the detection of gamma rays from clusters of galaxies, or even upper limits on their emission, will allow us to constrain the cosmic ray output of the sources they contain, such as normal galaxies, AGNs, and cosmological shocks. In this paper, we describe the impact that the Cherenkov Telescope Array, a future ground-based facility for very-high energy gamma-ray astronomy, is expected to have in this field of research.Comment: accepted to Astroparticle Physics, special issue on Physics with the Cherenkov Telescope Arra

Experimental analysis of concentrated versus distributed massive MIMO in an indoor cell at 3.5 GHz

This paper presents a measurement-based comparison between distributed and concentrated massive multiple-input multiple-output (MIMO) systems, which are called D-mMIMO and C-mMIMO systems, in an indoor environment considering a 400 MHz bandwidth centered at 3.5 GHz. In both cases, we have considered an array of 64 antennas in the base station and eight simultaneously active users. The work focuses on the characterization of both schemes in the up-link, considering the analysis of the sum capacity, the total spectral efficiency (SE) achievable by using the zero forcing (ZF) combining method, as well as the user fairness. The effect of the power imbalance between the different transmitters or user terminal (UT) locations, and thus, the benefits of performing an adequate power control are also investigated. The differences between the C-mMIMO and D-mMIMO channel performances are explained through the observation of the structure of their respective measured channel matrices through parameters such as the condition number or the power imbalance between the channels established by each UT. The channel measurements have been performed in the frequency domain, emulating a massive MIMO system in the framework of a time-domain duplex orthogonal frequency multiple access network (TDD-OFDM-MIMO). The characterization of the MIMO channel is based on the virtual array technique for both C-mMIMO and D-mMIMO systems. The deployment of the C-mMIMO and D-MIMO systems, as well as the distribution of users in the measurement environment, has been arranged as realistically as possible, avoiding the movement of people or machines.This work has been supported by the Spanish Ministry of Economy, Industry and Competitiveness (TEC2017-86779-C2-1-R)

Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology

Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified G. lucidum was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the G. lucidum strain QRS 5120 generated 637 base pairs, which was commensurate with related Ganoderma species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (p lt 0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (p lt 0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using G. lucidum

Binaries with the eyes of CTA

The binary systems that have been detected in gamma rays have proven very useful to study high-energy processes, in particular particle acceleration, emission and radiation reprocessing, and the dynamics of the underlying magnetized flows. Binary systems, either detected or potential gamma-ray emitters, can be grouped in different subclasses depending on the nature of the binary components or the origin of the particle acceleration: the interaction of the winds of either a pulsar and a massive star or two massive stars; accretion onto a compact object and jet formation; and interaction of a relativistic outflow with the external medium. We evaluate the potentialities of an instrument like the Cherenkov telescope array (CTA) to study the non-thermal physics of gamma-ray binaries, which requires the observation of high-energy phenomena at different time and spatial scales. We analyze the capability of CTA, under different configurations, to probe the spectral, temporal and spatial behavior of gamma-ray binaries in the context of the known or expected physics of these sources. CTA will be able to probe with high spectral, temporal and spatial resolution the physical processes behind the gamma-ray emission in binaries, significantly increasing as well the number of known sources. This will allow the derivation of information on the particle acceleration and emission sites qualitatively better than what is currently available.Comment: 23 pages, 13 figures, accepted for publication in Astroparticle Physics, special issue on Physics with the Cherenkov Telescope Arra

Is there a role for melatonin in fibromyalgia?

Fibromyalgia, characterised by persistent pain, fatigue, sleep disturbance and cognitive dysfunction, is a central sensitivity syndrome that also involves abnormality in peripheral generators and in the hypothalamic pituitary adrenal axis. Heterogeneity of clinical expression of fibromyalgia with a multifactorial aetiology has made the development of effective therapeutic strategies challenging. Physiological properties of the neurohormone melatonin appear related to the symptom profile exhibited by patients with fibromyalgia and thus disturbance of it’s production would be compatible with the pathophysiology. Altered levels of melatonin have been observed in patients with fibromyalgia which are associated with lower secretion during dark hours and higher secretion during daytime. However, inconsistencies of available clinical evidence limit conclusion of a relationship between levels of melatonin and symptom profiles in patients with fibromyalgia. Administration of melatonin to patients with fibromyalgia has demonstrated suppression of many symptoms and an improved quality of life consistent with benefit as a therapy for the management of this condition. Further studies with larger samples, however, are required to explore the potential role of melatonin in the pathophysiology of fibromyalgia and determine the optimal dosing regimen of melatonin for the management of fibromyalgia