In Situ Hybridization of 70 kD Heat Shock Protein mRNA in a Rat Model of Ethanol Self-Administration

Sucrose fading was used to initiate self-administration of ethanol on an FR4 schedule in male Fischer 344 rats. Rats showed low response rates for ethanol alone. After administration of liquid diet containing ethanol, ethanol intake increased over levels prior to administration of the liquid diet. In situ hybridization compared mRNA for the inducible or constitutive 70 kD heat shock proteins in ethanol and nonethanol rats. Both inducible and constitutive mRNAs were found in nonethanol and ethanol tissues. In peripheral organs, radiolableling was higher in ethanol tissue. In brain regions, nonethanol tissues showed higher radiolabeling

Reflexive use of methods: a framework for navigating different types of knowledge and power in transformative research

In transformative sustainability science, reflexivity is considered critical for ethically sound and socially relevant research. In practice, many transdisciplinary knowledge co-production processes have faced problems in mitigating power hierarchies among the participating actors and the different types of knowledge. In this paper, we develop and test a reflexive framework that enables transdisciplinary researchers to convey more explicitly how their methodological choices play a role in im/balancing power relations in knowledge co-production. The reflexive framework allows researchers to distinguish the different types of knowledge co-produced by the methods, as well as tracking the movements between them. We utilize the framework to reflect upon the methodological choices made through the application of three different transformative methods, namely the Transition Arena, Theory of Change, and Participatory Food Sustainability Assessment and Transformation Framework in different contexts. The results illuminate how the agility between the knowledge types is critical for navigating tensions in power imbalances, as well as producing transformative knowledge. Moreover, the results call further attention to the co-production of critical knowledge in sustainability science

Robotic observation pipeline for small bodies in the solar system based on open-source software and commercially available telescope hardware

The observation of small bodies in the Space Environment is an ongoing important task in astronomy. While nowadays new objects are mostly detected in larger sky surveys, several follow-up observations are usually needed for each object to improve the accuracy of orbit determination. In particular objects orbiting close to Earth, so called Near-Earth Objects (NEOs) are of special concern as a small but not negligible fraction of them can have a non-zero impact probability with Earth. Additionally, the observation of manmade space debris and tracking of satellites falls in the same class measurements. Telescopes for these follow-up observations are mainly in a aperture class between 1 m down to approximately 25 cm. These telescopes are often hosted by amateur observatories or dedicated companies like 6ROADS specialized on this type of observation. With upcoming new NEO search campaigns by very wide field of view telescopes, like the Vera C. Rubin Observatory, NASA’s NEO surveyor space mission and ESA’s Flyeye telescopes, the number of NEO discoveries will increase dramatically. This will require an increasing number of useful telescopes for follow-up observations at different geographical locations. While well-equipped amateur astronomers often host instruments which might be capable of creating useful measurements, both observation planning and scheduling, and also analysis are still a major challenge for many observers. In this work we present a fully robotic planning, scheduling and observation pipeline that extends the widely used open-source cross-platform software KStars/Ekos for Instrument Neutral Distributed Interface (INDI) devices. The method consists of algorithms which automatically select NEO candidates with priority according to ESA’s Near-Earth Object Coordination Centre (NEOCC). It then analyses detectable objects (based on limiting magnitudes, geographical position, and time) with preliminary ephemeris from the Minor Planet Center (MPC). Optimal observing slots during the night are calculated and scheduled. Immediately before the measurement the accurate position of the minor body is recalculated and finally the images are taken. Besides the detailed description of all components, we will show a complete robotic hard- and software solution based on our methods.TS-R acknowledges funding from the NEO-MAPP project (H2020-EU-2-1-6/870377). This work was (partially) funded by the Spanish MICIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” by the “European Union” through grant RTI2018-095076-B-C21, and the Institute of Cosmos Sciences University of Barcelona (ICCUB, Unidad de Excelencia “María de Maeztu”) through grant CEX2019-000918-M

Biosignature stability in space enables their use for life detection on Mars

Two rover missions to Mars aim to detect biomolecules as a sign of extinct or extant life with, among other instruments, Raman spectrometers. However, there are many unknowns about the stability of Raman-detectable biomolecules in the martian environment, clouding the interpretation of the results. To quantify Raman-detectable biomolecule stability, we exposed seven biomolecules for 469 days to a simulated martian environment outside the International Space Station. Ultraviolet radiation (UVR) strongly changed the Raman spectra signals, but only minor change was observed when samples were shielded from UVR. These findings provide support for Mars mission operations searching for biosignatures in the subsurface. This experiment demonstrates the detectability of biomolecules by Raman spectroscopy in Mars regolith analogs after space exposure and lays the groundwork for a consolidated space-proven database of spectroscopy biosignatures in targeted environments

Tracking smell loss to identify healthcare workers with SARS-CoV-2 infection

Introduction Healthcare workers (HCW) treating COVID-19 patients are at high risk for infection and may also spread infection through their contact with vulnerable patients. Smell loss has been associated with SARS-CoV-2 infection, but it is unknown whether monitoring for smell loss can be used to identify asymptomatic infection among high risk individuals. In this study we sought to determine if tracking smell sensitivity and loss using an at-home assessment could identify SARS-CoV-2 infection in HCW. Methods and findings We performed a prospective cohort study tracking 473 HCW across three months to determine if smell loss could predict SARS-CoV-2 infection in this high-risk group. HCW subjects completed a longitudinal, behavioral at-home assessment of olfaction with household items, as well as detailed symptom surveys that included a parosmia screening questionnaire, and real-time quantitative polymerase chain reaction testing to identify SARS-CoV-2 infection. Our main measures were the prevalence of smell loss in SARS-CoV-2-positive HCW versus SARS-CoV- 2-negative HCW, and timing of smell loss relative to SARS-CoV-2 test positivity. SARS-CoV-2 was identified in 17 (3.6%) of 473 HCW. HCW with SARS-CoV-2 infection were more likely to report smell loss than SARS-CoV-2-negative HCW on both the at-home assessment and the screening questionnaire (9/17, 53% vs 105/456, 23%, P < .01). 6/9 (67%) of SARS-CoV-2-positive HCW reporting smell loss reported smell loss prior to having a positive SARS-CoV-2 test, and smell loss was reported a median of two days before testing positive. Neurological symptoms were reported more frequently among SARS-CoV-2-positive HCW who reported smell loss compared to those without smell loss (9/9, 100% vs 3/8, 38%, P < .01). Conclusions In this prospective study of HCW, self-reported changes in smell using two different measures were predictive of SARS-CoV-2 infection. Smell loss frequently preceded a positive test and was associated with neurological symptoms

Negation and the functional sequence

There exists a general restriction on admissible functional sequences which prevents adjacent identical heads. We investigate a particular instantiation of this restriction in the domain of negation. Empirically, it manifests itself as a restriction the stacking of multiple negative morphemes. We propose a principled account of this restriction in terms of the general ban on immediately consecutive identical heads in the functional sequence on the one hand, and the presence of a Neg feature inside negative morphemes on the other hand. The account predicts that the stacking of multiple negative morphemes should be possible provided they are separated by intervening levels of structure. We show that this prediction is borne out

Biosignature stability in space enables their use for life detection on Mars

Two rover missions to Mars aim to detect biomolecules as a sign of extinct or extant life with, among other instruments, Raman spectrometers. However, there are many unknowns about the stability of Raman-detectable biomolecules in the martian environment, clouding the interpretation of the results. To quantify Raman-detectable biomolecule stability, we exposed seven biomolecules for 469 days to a simulated martian environment outside the International Space Station. Ultraviolet radiation (UVR) strongly changed the Raman spectra signals, but only minor change was observed when samples were shielded from UVR. These findings provide support for Mars mission operations searching for biosignatures in the subsurface. This experiment demonstrates the detectability of biomolecules by Raman spectroscopy in Mars regolith analogs after space exposure and lays the groundwork for a consolidated space-proven database of spectroscopy biosignatures in targeted environments

Evaluating the Effects of SARS-CoV-2 Spike Mutation D614G on Transmissibility and Pathogenicity.

Global dispersal and increasing frequency of the SARS-CoV-2 spike protein variant D614G are suggestive of a selective advantage but may also be due to a random founder effect. We investigate the hypothesis for positive selection of spike D614G in the United Kingdom using more than 25,000 whole genome SARS-CoV-2 sequences. Despite the availability of a large dataset, well represented by both spike 614 variants, not all approaches showed a conclusive signal of positive selection. Population genetic analysis indicates that 614G increases in frequency relative to 614D in a manner consistent with a selective advantage. We do not find any indication that patients infected with the spike 614G variant have higher COVID-19 mortality or clinical severity, but 614G is associated with higher viral load and younger age of patients. Significant differences in growth and size of 614G phylogenetic clusters indicate a need for continued study of this variant