Construction and characterization of a partial binary bacterial artificial chromosome (BIBAC) of Agave tequilana var. azul (2X) and its application for gene identification

The structure and organization of the genome of Agave is still unknown. To provide a genomic tool for searching sequences of the genus, we built and characterized a binary (BIBAC2) genomic library of Agave tequilana  Weber var. azul. Clones of the library had an average insert size of 170 Kb. The frequency of inserts with  internal Not I sites was 30% and only 5% of the library showed organelle contamination. The library was  assessed using probes with high homology to repeated regions (retroelements and rDNA regions), genes  involved in the resistance to diseases (NBS-LRR) and genes related to late embryogenesis (LEA).  Recombinant clones that hybridized with each of the probes were identified. Our results indicate that the  obtained genomic library is suitable for the identification of sequences of interest, for genetic mapping and for studies of gene regulation and expression.Key words: Binary bacterial artificial chromosome (BIBAC), Agave tequilana, genome, clones

Análisis bibliométrico sobre metodologías de costeo de proyectos de la industria creativa

Objective. To analyze the research trends in relation to the costing of creative economy projects. Methods. A bibliometric analysis was developed from the Scopus database, applying a search equation and calculating bibliometric indicators such as quantity, quality and identification of emerging topics from documents published between 1970 and 2020. Results. The creative industries has been a topic of interest to researchers since 1997; however, it is possible to observe a continuous growth from the year 2001, date that coincides with the first publications made by Professor John Howkins, in the same way it is identified that from the social sciences show a greater interest in the subject. Conclusions. There is evidence of an impact and growth in the area of study, which validates the convenience of research projects oriented to costing methodologies for creative industry projects.Objetivo. Analizar las tendencias investigativas en relación con el costeo de proyectos de la economía creativa. Métodos. Se desarrolló un análisis bibliométrico a partir de la base de datos Scopus, aplicando una ecuación de búsqueda y calculando indicadores de tipo bibliométricos como cantilidad, calidad e identificación de temas emergentes a partir de los documentos publicados entre 1970 y 2020. Resultados. Las industrias creativas ha sido un tema de interés de los investigadores desde el año 1997; sin embargo, se logra observar un crecimiento continuo a partir del año 2001, fecha que coincide con las primeras publicaciones realizadas por el profesor John Howkins, de igual forma se identifica que desde las ciencias sociales demuestran un mayor interés por la temática Conclusiones. Se evidencia un impacto y crecimiento del área de estudio, lo que valida la conveniencia de los proyectos de investigación orientados a las metodologías de costeo de proyectos de la industria creativa