Etude de l’effet antidépresseur de la décoction des rhizomes de Cyperus Articulatus (Cyperaceae) sur les souris blanches Mus musculus Swiss (Muridae)

La présente étude porte sur l’évaluation des effets pharmacologiques de la décoction des rhizomes de Cyperus Articulatus sur le système nerveux central. Cyperus Articulatus est une plante utilisée en médecine traditionnelle par certaines populations d’Asie et d’Afrique pour le traitement de plusieurs maladies telles que l’épilepsie, l’insomnie, les céphalées. Différentes doses (524 ; 262 ; 131 et 52,4 mg/kg) de la décoction des rhizomes C. Articulatus ont été administrées aux souris par voie orale et ces propriétés antidépressives ont été évaluées selon trois méthodes psychopharmacologiques à savoir : le test de la nage forcée ; le test de suspension caudale et le test de l’arène ouverte. L’administration de la décoction de C. Articulatus a entrainé une diminution significative de la durée d’immobilité dans le test de la nage forcée et le test de suspension caudale. Cette durée est passée respectivement de 232,40 ± 43,96 et 262,60 ± 84,00 secondes chez les souris du lot contrôle négatif à 150,00 ± 50,08 et 106,60 ± 56,86 secondes chez les souris traitées par la dose 524 mg/kg de la décoction. Dans le test de l’arène ouverte, nous n’avons pas noté une augmentation significative du nombre de rearing qui est de 16,60 ± 6,42 chez les souris du lot contrôle négatif et 21 ± 20 et 24,60 ± 9,23 secondes pour les doses 524 mg/kg de C. Articulatus et d’imipramine (20 mg/kg). Tous ces effets indiqueraient la présence dans cette décoction des rhizomes de C. Articulatus des composés qui posséderaient des propriétés antidépressives justifiant son utilisation en médecine traditionnelle. The present study reports the pharmacological effects of the decoction of Cyperus Articulatus rhizomes on the central nervous system. Cyperus Articulatus is a plant used in traditional medicine by some populations of Asia and Africa for the treatment of several diseases such as epilepsy, insomnia, and headache. The decoction of C. Articulatus were orally administered to mice at various doses (524; 262; 131 and 52.4 mg/kg) and its anti-depressive properties evaluated according to different psycho-pharmacological methods (forced swimming test, tail suspension test, and open field test). The administration of the decoction of C. Articulatus to the mice led to a significant reduction in the duration of immobility in the forced swimming test and the tail suspension test. This duration reduced respectively from 232.40 ± 43.96 and 262.60 ± 84.00 seconds in the mice of the negative controls batch to 150.00 ± 50.08 and 106.60 ± 56.86 seconds in the mice treated with the dose of 524 mg/kg of the decoction. In the open field test, we did not notice a significant increase in the number of rearing positions; these values were 16.60 ± 6.42 seconds in the negative control batch mice and only 24.60 ± 9.23 and 21 ± 20 seconds for the positive control batch and the dose of 524 mg/kg of C. Articulatus respectively. All these effects indicate the presence of bioactive molecules in this decoction of the rhizomes of C Articulatus which would have anti-depressive properties justifying its use in traditional medicine

ITIL-Based IT Service Support Process Reengineering

The Information Technology Infrastructure Library (ITIL) supports best practices, reengineering activities and IT service support processes. ITIL framework only provides recommendations, and companies need to utilize this framework to improve their IT service support processes and establish best practices. This study provides a methodology on how to apply the ITIL framework for evaluating the IT service support processes, its reengineering and alignment to best practices, and subsequent integration into a decision support system framework. A case study approach was used to identify a set of Key Performance Indicators (KPI) which were monitored by a decision support system (DSS) for triggering on-going reengineering of IT service support processes. This paper focuses on the implementation of the ITIL guidelines at the operational level, improvement of the service desk, and incident, problem, change, release, and configuration management. It also presents the implementation of the ITIL guidelines at the tactical level for the improvement of the service level, capacity, IT service continuity, service availability, and security management. We conclude by providing recommendations for future research

PROPIEDADES PSICOMÉTRICAS DE LA ESCALA DEL TRASTORNO DE ANSIEDAD GENERALIZADA: GAD-7 EN UNIVERSITARIOS DE LIMA, PERÚ

La ansiedad generalizada en estudiantes universitarios merece mayor atención debido al incremento de la prevalencia y a la gran comorbilidad con otros trastornos de ansiedad, por lo cual es necesario contar con instrumentos válidos y fiables. El objetivo fue evaluar las propiedades psicométricas del GAD-7 en universitarios de Lima, Perú. Se realizó un estudio psicométrico instrumental, transversal, descriptivo correlacional, en 300 universitarios de Lima, de uno u otro sexo, entre 18 y 50 años, quienes completaron la Escala GAD-7 en clases virtuales. Los resultados muestran que los coeficientes de correlación ítem-test corregida van de .853 a .875. El análisis factorial permitió confirmar que existe una sola dimensión, además, el alfa de Cronbach fue .87 y el omega de McDonald’s .88. La ansiedad severa fue 22.6%

ANALYSIS OF FIRE DETECTION MODULE FOR AUTOMATIC SECURITY SYSTEM AND EXTINGUISHING ROBOT

In the late years, mechanical technology has ended up being a fixing over which numerous individuals had demonstrated their advantage. Apply autonomy has picked up prevalence because of the headway of numerous advancements of processing and nano advances. Along these lines, we proposed to plan something that can make people life less demanding and agreeable. This venture, which is our attempt to plan a putting out fires robot. Involves a machine which not just has the essential elements of a robot, additionally can recognize fire and smother it. The need of great importance is to make a gadget which can identify fire, regardless of the possibility that it is little and make the vital move to put it off. Numerous house hold thing burst into flames when somebody is either resting or away and that lead numerous risky conditions if the flame is not putted off in time. In this way, we fill in as a gadgets specialist is to plan and manufacture framework that can consequently identify and smother fire. We have utilized extremely fundamental idea here, straightforward from the forthcoming of apprentices or for the bosses of this field

The First Genomic and Proteomic Characterization of a Deep-Sea Sulfate Reducer: Insights into the Piezophilic Lifestyle of Desulfovibrio piezophilus

Desulfovibrio piezophilus strain C1TLV30T is a piezophilic anaerobe that was isolated from wood falls in the Mediterranean deep-sea. D. piezophilus represents a unique model for studying the adaptation of sulfate-reducing bacteria to hydrostatic pressure. Here, we report the 3.6 Mbp genome sequence of this piezophilic bacterium. An analysis of the genome revealed the presence of seven genomic islands as well as gene clusters that are most likely linked to life at a high hydrostatic pressure. Comparative genomics and differential proteomics identified the transport of solutes and amino acids as well as amino acid metabolism as major cellular processes for the adaptation of this bacterium to hydrostatic pressure. In addition, the proteome profiles showed that the abundance of key enzymes that are involved in sulfate reduction was dependent on hydrostatic pressure. A comparative analysis of orthologs from the non-piezophilic marine bacterium D. salexigens and D. piezophilus identified aspartic acid, glutamic acid, lysine, asparagine, serine and tyrosine as the amino acids preferentially replaced by arginine, histidine, alanine and threonine in the piezophilic strain. This work reveals the adaptation strategies developed by a sulfate reducer to a deep-sea lifestyle

In-situ fluorescence spectroscopy indicates total bacterial abundance and dissolved organic carbon

We explore in-situ fluorescence spectroscopy as an instantaneous indicator of total bacterial abundance and faecal contamination in drinking water. Eighty-four samples were collected outside of the recharge season from groundwater-derived water sources in Dakar, Senegal. Samples were analysed for tryptophan-like (TLF) and humic-like (HLF) fluorescence in-situ, total bacterial cells by flow cytometry, and potential indicators of faecal contamination such as thermotolerant coliforms (TTCs), nitrate, and in a subset of 22 samples, dissolved organic carbon (DOC). Significant single-predictor linear regression models demonstrated that total bacterial cells were the most effective predictor of TLF, followed by on-site sanitation density; TTCs were not a significant predictor. An optimum multiple-predictor model of TLF incorporated total bacterial cells, nitrate, nitrite, on-site sanitation density, and sulphate (r2 0.68). HLF was similarly related to the same parameters as TLF, with total bacterial cells being the best correlated (ρs 0.64). In the subset of 22 sources, DOC clustered with TLF, HLF, and total bacterial cells, and a linear regression model demonstrated HLF was the best predictor of DOC (r2 0.84). The intergranular nature of the aquifer, timing of the study, and/or non-uniqueness of the signal to TTCs can explain the significant associations between TLF/HLF and indicators of faecal contamination such as on-site sanitation density and nutrients but not TTCs. The bacterial population that relates to TLF/HLF is likely to be a subsurface community that develops in-situ based on the availability of organic matter originating from faecal sources. In-situ fluorescence spectroscopy instantly indicates a drinking water source is impacted by faecal contamination but it remains unclear how that relates specifically to microbial risk in this setting

The Mechanisms of Codon Reassignments in Mitochondrial Genetic Codes

Many cases of non-standard genetic codes are known in mitochondrial genomes. We carry out analysis of phylogeny and codon usage of organisms for which the complete mitochondrial genome is available, and we determine the most likely mechanism for codon reassignment in each case. Reassignment events can be classified according to the gain-loss framework. The gain represents the appearance of a new tRNA for the reassigned codon or the change of an existing tRNA such that it gains the ability to pair with the codon. The loss represents the deletion of a tRNA or the change in a tRNA so that it no longer translates the codon. One possible mechanism is Codon Disappearance, where the codon disappears from the genome prior to the gain and loss events. In the alternative mechanisms the codon does not disappear. In the Unassigned Codon mechanism, the loss occurs first, whereas in the Ambiguous Intermediate mechanism, the gain occurs first. Codon usage analysis gives clear evidence of cases where the codon disappeared at the point of the reassignment and also cases where it did not disappear. Codon disappearance is the probable explanation for stop to sense reassignments and a small number of reassignments of sense codons. However, the majority of sense to sense reassignments cannot be explained by codon disappearance. In the latter cases, by analysis of the presence or absence of tRNAs in the genome and of the changes in tRNA sequences, it is sometimes possible to distinguish between the Unassigned Codon and Ambiguous Intermediate mechanisms. We emphasize that not all reassignments follow the same scenario and that it is necessary to consider the details of each case carefully.Comment: 53 pages (45 pages, including 4 figures + 8 pages of supplementary information). To appear in J.Mol.Evo