Influence of composting techniques on microbial succession, temperature and pH in a composting municipal solid waste

Composting of urban wastes was carried out using both passive aeration technique (PACT) and conventional pit method. Feacal coliforms, Pseudomonas, Streptococcus, Proteus , Seratia and Bacillus species as well as fungi were isolated at mesophilic stage of degradation. A number of these microorganisms did not grow at the thermophilic stage but grew at cooling down stage. The trends in microbial succession in the composting wastes in pot and pit were somewhat similar. There was, however, repeated re-heat after turning the wastes in the pit until about 5 months later. Each time the waste was turned in the pit, there was an increase in temperature until the 21st week. Temperature however, stabilized at the 7th week in the pot. pH also stabilized as the composting process progressed in the pit. Good quality compost was obtained in 5 weeks when PACT was used. Conventional pit method lasted over several weeks. Key Words: Municipal wastes; passive aeration; pit composting; temperature; microbial succession. African Journal of Biotechnology Vol. 3 (4), 2004: 239-24

Comparative effects of Apron plus 50DS and soil amendment on the growth, yield and food components of soybean

Comparative effects of Apron plus 50DS (a systemic fungicide) and soil amendment (poultry droppings) on growth yield and food components of soybean (TGX 1485-1D) were studied. Soybean seeds treated withrecommended dose of Apron plus 50DS had lower percentage germination when compared to the other treatments while the untreated seeds (control) had the highest percentage germination. Seeds treated with Apron plus and planted on amended soil gave the best performance in terms of development and yield. Biochemical analysis of harvested seeds showed an increase in protein content of seeds treated with recommended dose of Apron plus, planted on amended soil. Seeds treated with less than the recommended dose had the highest percentage carbohydrate content, while untreated seeds planted on amended soil had the lowest carbohydrate content. The control experiment had the highest percentage crude fibre while the lowestwas recorded for seeds treated with recommended dose of Apron plus, planted on amended soil. However, there is no significant difference among the treatments in percentage ash and moisture content

Evaluation of fallow and cover crops for nematode suppression in three agroecologies of south western Nigeria

A study was conducted in three agroecological zones of south-western Nigeria to evaluate the effect of siam weed (Chromolaena odorata) and mucuna (Mucuna utilis) cover/fallow crops on plant-parasitic nematode population. The natural bush regrowth was used as control. Plant-parasitic nematodes were identified and counted during the fallow periods. Eleven genera of nematodes were identified and three (Meloidogyne, Pratylenchus, and Helicotylenchus) species were predominant across the trial locations.Other important genera present were Scutellonema, Tylenchorhynchus and Rotylenchus species. Nematode population densities of pre-crop were lowest in Alagba soil (Rhodic lixisols) at Ikenne in the wet forest zone of Nigeria. The populations in Iwo soil (Rhodic haplustalf) at Ibadan, a dry forest zonewas lower than in the Temidire soil (Plinthic luvisol) at Ilora in the derived savannah of south-western Nigeria. There was significant suppression of nematode population densities under the different crops as the fallow period increased. The population reduction in the different locations depended on the nematode species and the cover crops. The natural bush re-growth had the least effect on the nematode suppression at the end of the fallow period. On the average, siam weed fallow reduced nematode population densities by 67-79%, mucuna by 64-72% and the natural bush by 30-49% across the trial locations. For effective nematode suppression with fallow cropping, proper determination of the principal nematode species predominant in an environment is essential

Post-consent assessment of dental subjects' understanding of informed consent in oral health research in Nigeria

Abstract Background Research participants may not adequately understand the research in which they agree to enroll. This could be due to a myriad of factors. Such a missing link in the informed consent process contravenes the requirement for an "informed" consent prior to the commencement of research. This study assessed the post consent understanding of Nigerian study participants of the oral health research they were invited to join. Methods A descriptive cross sectional study with research participants who had just consented to one of three ongoing research studies on oral health. Study sites included two centers, one in the northern and one in the southern part of Nigeria. Data were collected using a combination of quantitative and qualitative methods. Results A total of 113 research participants were interviewed. The southern part of the country had 58 respondents with the north having 55. The age range was 21 – 80 years. Mean age was 46.1 (SD16.3). The sample was predominantly male (69.9%) and married (64.6%). There was poor understanding of some key elements of the informed consent process such as involvement in research, benefits, contacts, confidentiality and voluntariness. Some identified factors potentially compromising understanding were poverty, illiteracy, therapeutic misconception and confusion about the dual roles of the Dentist and the researcher. Conclusion The participants recruited into the oral health research in Nigeria did not adequately understand the studies they were invited to join nor do they understand their rights as research participants. Measures should be taken to include research bioethics into the curricula of Dental schools and to train oral health researchers in the country on research ethics.</p

Repeated Assessments of Informed Consent Comprehension among HIV-Infected Participants of a Three-Year Clinical Trial in Botswana

Informed consent (IC) has been an international standard for decades for the ethical conduct of clinical trials. Yet frequently study participants have incomplete understanding of key issues, a problem exacerbated by language barriers or lack of familiarity with research concepts. Few investigators measure participant comprehension of IC, while even fewer conduct interim assessments once a trial is underway.We assessed comprehension of IC using a 20-question true/false quiz administered in 6-month intervals in the context of a placebo-controlled, randomized trial for the prevention of tuberculosis among HIV-infected adults in Botswana (2004-2009). Quizzes were offered in both Setswana and English. To enroll in the TB trial, participants were required to have ≥ 16/20 correct responses. We examined concepts understood and the degree to which understanding changed over three-years. We analyzed 5,555 quizzes from 1,835 participants. The participants' highest education levels were: 28% primary, 59% secondary, 9% tertiary and 7% no formal education. Eighty percent of participants passed the enrollment quiz (Quiz1) on their first attempt and the remainder passed on their second attempt. Those having higher than primary education and those who took the quiz in English were more likely to receive a passing score on their first attempt (adjusted odds ratios and 95% confidence intervals, 3.1 (2.4-4.0) and 1.5 (1.2, 1.9), respectively). The trial's purpose or procedures were understood by 90-100% of participants, while 44-77% understood randomization, placebos, or risks. Participants who failed Quiz1 on their initial attempt were more likely to fail quizzes later in the trial. Pass rates improved with quiz re-administration in subsequent years.Administration of a comprehension quiz at enrollment and during follow-up was feasible in a large, international collaboration and efficiently determined IC comprehension by trial participants. Strategies to improve understanding of concepts like placebos and randomization are needed. Comprehension assessments throughout a study may reinforce key concepts

Pooled analysis of WHO Surgical Safety Checklist use and mortality after emergency laparotomy

Background The World Health Organization (WHO) Surgical Safety Checklist has fostered safe practice for 10 years, yet its place in emergency surgery has not been assessed on a global scale. The aim of this study was to evaluate reported checklist use in emergency settings and examine the relationship with perioperative mortality in patients who had emergency laparotomy. Methods In two multinational cohort studies, adults undergoing emergency laparotomy were compared with those having elective gastrointestinal surgery. Relationships between reported checklist use and mortality were determined using multivariable logistic regression and bootstrapped simulation. Results Of 12 296 patients included from 76 countries, 4843 underwent emergency laparotomy. After adjusting for patient and disease factors, checklist use before emergency laparotomy was more common in countries with a high Human Development Index (HDI) (2455 of 2741, 89.6 per cent) compared with that in countries with a middle (753 of 1242, 60.6 per cent; odds ratio (OR) 0.17, 95 per cent c.i. 0.14 to 0.21, P <0001) or low (363 of 860, 422 per cent; OR 008, 007 to 010, P <0.001) HDI. Checklist use was less common in elective surgery than for emergency laparotomy in high-HDI countries (risk difference -94 (95 per cent c.i. -11.9 to -6.9) per cent; P <0001), but the relationship was reversed in low-HDI countries (+121 (+7.0 to +173) per cent; P <0001). In multivariable models, checklist use was associated with a lower 30-day perioperative mortality (OR 0.60, 0.50 to 073; P <0.001). The greatest absolute benefit was seen for emergency surgery in low- and middle-HDI countries. Conclusion Checklist use in emergency laparotomy was associated with a significantly lower perioperative mortality rate. Checklist use in low-HDI countries was half that in high-HDI countries.Peer reviewe