Direct detection of galaxy stellar halos : NGC 3957 as a test case

We present a direct detection of the stellar halo of the edge-on S0 galaxy NGC 3957, using ultra-deep VLT/VIMOS V and R images. This is achieved with a sky subtraction strategy based on infrared techniques. These observations allow us to reach unprecedented high signal-to-noise ratios up to 15 kpc away from the galaxy center, rendering photon-noise negligible. The 1 sigma detection limits are R = 30.6 mag/arcsec^2 and V = 31.4 mag/arcsec^2. We conduct a thorough analysis of the possible sources of systematic errors that could affect the data: flat-fielding, differences in CCD responses, scaling of the sky background, the extended halo itself, and PSF wings. We conclude that the V-R colour of the NGC 3957 halo, calculated between 5 and 8 kpc above the disc plane where the systematic errors are modest, is consistent with an old and preferentially metal-poor normal stellar population, like that revealed in nearby galaxy halos from studies of their resolved stellar content. We do not find support for the extremely red colours found in earlier studies of diffuse halo emission, which we suggest might have been due to residual systematic errors.Comment: Accepted for publication in A&A - "language edited

Fibroblast activation protein-α in fibrogenic disorders and cancer: more than a prolyl-specific peptidase?

Fibroblast activation protein-α (FAP-α) belongs to the family of prolyl-specific serine proteases. FAP-α displays both exopeptidase and endopeptidase/gelatinase/collagenase activities. FAP-α protein and/or activity have been associated with fibrosis, inflammation and cancer, but the protein is undetectable in most normal tissues. FAP-α is selectively expressed at sites of tissue remodeling and repair and enhances tumor progression, suggesting that this protease may be a therapeutic target to treat human disorders associated with fibrotic dysregulation. Areas covered: In this review, we summarize the mechanisms driving tissue fibrosis and describe some of the enzymes involved in fibrosis, concentrating on FAP-α. We describe its enzymatic properties, discuss the tools developed to control its activity and the problem of selectivity toward the other proteases of the family and outline its potential biological substrates. We also consider non-enzymatic functions of this protein and suggest that repression of FAP-α expression may represent therapeutic options. Expert opinion: Questions remain regarding the biological functions of FAP-α, either dependent or independent of its enzyme activity. However, as progress is underway to develop FAP-α-specific inhibitors and therapeutic antibodies, its role in diseases associated with fibrosis is starting to emerge, ultimately leading to novel therapeutic options for inflammatory and oncologic diseases

Chemical Composition of Extremely Metal-Poor Stars in the Sextans Dwarf Spheroidal Galaxy

Chemical abundances of six extremely metal-poor ([Fe/H]<-2.5) stars in the Sextans dwarf spheroidal galaxy are determined based on high resolution spectroscopy (R=40,000) with the Subaru Telescope High Dispersion Spectrograph. (1) The Fe abundances derived from the high resolution spectra are in good agreement with the metallicity estimated from the Ca triplet lines in low resolution spectra. The lack of stars with [Fe/H]=<-3 in Sextans, found by previous estimates from the Ca triplet, is confirmed by our measurements, although we note that high resolution spectroscopy for a larger sample of stars will be necessary to estimate the true fraction of stars with such low metallicity. (2) While one object shows an overabundance of Mg (similar to Galactic halo stars), the Mg/Fe ratios of the remaining five stars are similar to the solar value. This is the first time that low Mg/Fe ratios at such low metallicities have been found in a dwarf spheroidal galaxy. No evidence for over-abundances of Ca and Ti are found in these five stars, though the measurements for these elements are less certain. Possible mechanisms to produce low Mg/Fe ratios, with respect to that of Galactic halo stars, are discussed. (3) Ba is under-abundant in four objects, while the remaining two stars exhibit large and moderate excesses of this element. The abundance distribution of Ba in this galaxy is similar to that in the Galactic halo, indicating that the enrichment of heavy elements, probably by the r-process, started at metallicities [Fe/H] < -2.5, as found in the Galactic halo.Comment: 15 pages, 6 figures, 6 tables, A&A, in pres

Patellins 3 and 6, two members of the Plant Patellin family, interact with the movement protein of Alfalfa mosaic virus and interfere with viral movement

This is the accepted version of the following article: Peiró Morell, A.; Izquierdo Garcia, AC.; Sanchez Navarro, JA.; Pallás Benet, V.; Mulet Salort, JM.; Aparicio Herrero, F. (2014). Patellins 3 and 6, two members of the Plant Patellin family, interact with the movement protein of Alfalfa mosaic virus and interfere with viral movement. Molecular Plant Pathology. 15(9):881-891. doi:10.1111/mpp.12146., which has been published in final form at http://dx.doi.org/10.1111/mpp.12146.[EN] Movement proteins (MPs) encoded by plant viruses interact with host proteins to facilitate or interfere with intra- and/or intercellular viral movement. Using yeast two-hybrid and bimolecular fluorescence complementation assays, we herein present invivo evidence for the interaction between Alfalfa mosaic virus (AMV) MP and Arabidopsis Patellin 3 (atPATL3) and Patellin 6 (atPATL6), two proteins containing a Sec14 domain. Proteins with Sec14 domains are implicated in membrane trafficking, cytoskeleton dynamics, lipid metabolism and lipid-mediated regulatory functions. Interestingly, the overexpression of atPATL3 and/or atPATL6 interfered with the plasmodesmata targeting of AMV MP and correlated with reduced infection foci size. Consistently, the viral RNA levels increased in the single and double Arabidopsis knockout mutants for atPATL3 and atPATL6. Our results indicate that, in general, MP-PATL interactions interfere with the correct subcellular targeting of MP, thus rendering the intracellular transport of viral MP-containing complexes less efficient and diminishing cell-to-cell movement.AP was a recipient of a Pre-Doctoral Fellowship from the program JAE Pre-Doc of Consejo superior de Investigaciones Cientificas. ACI-G was a recipient of a Pre-Doctoral Fellowship associated with the project BFU2008-00604. FA was a recipient of a contract Ramon y Cajal (RYC-2010-06169) Program of the Ministerio de Educacion y Ciencia of Spain. We thank L. Corachan for excellent technical assistance. This work was supported by grants BIO2011-25018 from the Direccion General de Investigacion Cientifica y Tecnica, the Prometeo Program GV2011/003 from the Generalitat Valenciana and PAID-06-10-1496 from the Universitat Politecnica de Valencia (Spain).Peiró Morell, A.; Izquierdo García, AC.; Sanchez Navarro, JA.; Pallás Benet, V.; Mulet Salort, JM.; Aparicio Herrero, F. (2014). Patellins 3 and 6, two members of the Plant Patellin family, interact with the movement protein of Alfalfa mosaic virus and interfere with viral movement. Molecular Plant Pathology. 15(9):881-891. https://doi.org/10.1111/mpp.12146S88189115

Direct detection of galaxy stellar halos : NGC 3957 as a test case

We present a direct detection of the stellar halo of the edge-on S0 galaxy NGC 3957, using ultra-deep VLT/VIMOS V and R images. This is achieved with a sky subtraction strategy based on infrared techniques. These observations allow us to reach unprecedented high signal-to-noise ratios up to 15 kpc away from the galaxy center, rendering photon-noise negligible. The 1 sigma detection limits are R = 30.6 mag/arcsec^2 and V = 31.4 mag/arcsec^2. We conduct a thorough analysis of the possible sources of systematic errors that could affect the data: flat-fielding, differences in CCD responses, scaling of the sky background, the extended halo itself, and PSF wings. We conclude that the V-R colour of the NGC 3957 halo, calculated between 5 and 8 kpc above the disc plane where the systematic errors are modest, is consistent with an old and preferentially metal-poor normal stellar population, like that revealed in nearby galaxy halos from studies of their resolved stellar content. We do not find support for the extremely red colours found in earlier studies of diffuse halo emission, which we suggest might have been due to residual systematic errors.Comment: Accepted for publication in A&A - "language edited

Manganese in dwarf spheroidal galaxies

We provide manganese abundances (corrected for the effect of the hyperfine structure) for a large number of stars in the dwarf spheroidal galaxies Sculptor and Fornax, and for a smaller number in the Carina and Sextans dSph galaxies. Abundances had already been determined for a number of other elements in these galaxies, including alpha and iron-peak ones, which allowed us to build [Mn/Fe] and [Mn/alpha] versus [Fe/H] diagrams. The Mn abundances imply sub-solar [Mn/Fe] ratios for the stars in all four galaxies examined. In Sculptor, [Mn/Fe] stays roughly constant between [Fe/H]\sim -1.8 and -1.4 and decreases at higher iron abundance. In Fornax, [Mn/Fe] does not vary in any significant way with [Fe/H]. The relation between [Mn/alpha] and [Fe/H] for the dSph galaxies is clearly systematically offset from that for the Milky Way, which reflects the different star formation histories of the respective galaxies. The [Mn/alpha] behavior can be interpreted as a result of the metal-dependent Mn yields of type II and type Ia supernovae. We also computed chemical evolution models for star formation histories matching those determined empirically for Sculptor, Fornax, and Carina, and for the Mn yields of SNe Ia, which were assumed to be either constant or variable with metallicity. The observed [Mn/Fe] versus [Fe/H] relation in Sculptor, Fornax, and Carina can be reproduced only by the chemical evolution models that include a metallicity-dependent Mn yield from the SNe Ia.Comment: 19 pages, 10 figures, accepted for publication in Astronomy & Astrophysic

The NIR Ca II triplet at low metallicity:Searching for extremely low-metallicity stars in classical dwarf galaxies

The NIR Ca II triplet absorption lines have proven to be an important tool for quantitative spectroscopy of individual red giant branch stars in the Local Group, providing a better understanding of metallicities of stars in the Milky Way and dwarf galaxies and thereby an opportunity to constrain their chemical evolution processes. An interesting puzzle in this field is the significant lack of extremely metal-poor stars, below [Fe/H] = -3, found in classical dwarf galaxies around the Milky Way using this technique. The question arises whether these stars are really absent, or if the empirical Ca II triplet method used to study these systems is biased in the low-metallicity regime. Here we present results of synthetic spectral analysis of the Ca II triplet, that is focused on a better understanding of spectroscopic measurements of low-metallicity giant stars. Our results start to deviate strongly from the widely-used and linear empirical calibrations at [Fe/H] &lt;-2. We provide a new calibration for Ca II triplet studies which is valid for -0.5 &gt;= [Fe/H] &gt;= -4. We subsequently apply this new calibration to current data sets and suggest that the classical dwarf galaxies are not so devoid of extremely low-metallicity stars as was previously thought.</p

Extremely metal-poor stars in classical dwarf spheroidal galaxies: Fornax, Sculptor and Sextans

We present the results of a dedicated search for extremely metal-poor stars in the Fornax, Sculptor and Sextans dSphs. Five stars were selected from two earlier VLT/Giraffe and HET/HRS surveys and subsequently followed up at high spectroscopic resolution with VLT/UVES. All of them turned out to have [Fe/H] <= -3 and three stars are below [Fe/H] -3.5. This constitutes the first evidence that the classical dSphs Fornax and Sextans join Sculptor in containing extremely metal-poor stars and suggests that all of the classical dSphs contain extremely metal-poor stars. One giant in Sculptor at [Fe/H]=-3.96 +- 0.10 is the most metal-poor star ever observed in an external galaxy. We carried out a detailed analysis of the chemical abundances of the alpha, iron peak, and the heavy elements, and we performed a comparison with the Milky Way halo and the ultra faint dwarf stellar populations. Carbon, barium and strontium show distinct features characterized by the early stages of galaxy formation and can constrain the origin of their nucleosynthesis.Comment: In A&A. This version corrects a few typographical errors in the coordinates of some of our stars (Table 1

Global Proteome Analysis of Leptospira interrogans

Comparative global proteome analyses were performed on Leptospira interrogans serovar Copenhageni grown under conventional in vitro conditions and those mimicking in vivo conditions (iron limitation and serum presence). Proteomic analyses were conducted using iTRAQ and LC-ESI-tandem mass spectrometry complemented with two-dimensional gel electrophoresis and MALDI-TOF mass spec-trometry. A total of 563 proteins were identified in this study. Altered expression of 65 proteins, including upregulation of the L. interrogans virulence factor Loa22 and 5 novel proteins with homology to virulence factors found in other pathogens, was observed between the comparative conditions. Immunoblot analyses confirmed upregulation of 5 of the known or putative virulence factors in L. interrogans exposed to the in vivo-like environmental conditions. Further, ELISA analyses using serum from patients with leptospirosis and immunofluorescence studies performed on liver sections derived from L. interrogans-infected hamsters verified expression of all but one of the identified proteins during infection. These studies, which represent the first documented comparative global proteome analysis of Leptospira, demonstrated proteome alterations under conditions that mimic in vivo infection and allowed for the identification of novel putative L. interrogans virulence factors

The Involvement of SMILE/TMTC3 in Endoplasmic Reticulum Stress Response

The state of operational tolerance has been detected sporadically in some renal transplanted patients that stopped immunosuppressive drugs, demonstrating that allograft tolerance might exist in humans. Several years ago, a study by Brouard et al. identified a molecular signature of several genes that were significantly differentially expressed in the blood of such patients compared with patients with other clinical situations. The aim of the present study is to analyze the role of one of these molecules over-expressed in the blood of operationally tolerant patients, SMILE or TMTC3, a protein whose function is still unknown.We first confirmed that SMILE mRNA is differentially expressed in the blood of operationally tolerant patients with drug-free long term graft function compared to stable and rejecting patients. Using a yeast two-hybrid approach and a colocalization study by confocal microscopy we furthermore report an interaction of SMILE with PDIA3, a molecule resident in the endoplasmic reticulum (ER). In accordance with this observation, SMILE silencing in HeLa cells correlated with the modulation of several transcripts involved in proteolysis and a decrease in proteasome activity. Finally, SMILE silencing increased HeLa cell sensitivity to the proteasome inhibitor Bortezomib, a drug that induces ER stress via protein overload, and increased transcript expression of a stress response protein, XBP-1, in HeLa cells and keratinocytes.In this study we showed that SMILE is involved in the endoplasmic reticulum stress response, by modulating proteasome activity and XBP-1 transcript expression. This function of SMILE may influence immune cell behavior in the context of transplantation, and the analysis of endoplasmic reticulum stress in transplantation may reveal new pathways of regulation in long-term graft acceptance thereby increasing our understanding of tolerance