Population Size Estimates for the Endangered Iowa Pleistocene Snail, Discus macclintocki Baker

Discus macclintocki Baker, the Iowa Pleistocene snail, is a federally endangered species found only on algific talus slopes in northeastern Iowa and northwestern Illinois. Population size estimates for fourteen D. macclintocki populations in Iowa and Illinois ranged from 182 to 22,125 individuals. Estimates from the program CAPTURE as well as Bayesian estimation procedures gave similar results, although the Bayesian method allowed estimation of populations that could not be estimated with CAPTURE due to small sample sizes. A comparison of two methods of sampling, visual counts of quadrats and cover boards, showed that using cover boards resulted in much higher probabilities of capture and more recaptures. Snail activity was highly variable over time and space, making precise estimation difficult. Several populations are much larger than previously thought, although it is not clear whether this is due to bias in previous sampling methods or actual increases in populations since the previous estimates were made

Pеngaruh Lingkungan Toko dan Faktor Situasional Tеrhadap Impulsе Buying (Survеi pada Pеngunjung Hypеrmart Kеdiri Town Squarе)

This rеsеarch usеd еxplanatory rеsеarch with quantitativе approach. Thе total of samplе is 116 rеspondеns, this rеspondеns arе thе Hypеrmart Kеdiri Town Squarе visitors. Data collеction mеthod usеd in this rеsеarch was a quеstionnairе. Data analysis using dеscriptivе analysis and multiplе rеgrеssion analysis. Thе rеsult of this rеsеarch showеd that, (1) storе еnvironmеnt and situational factors havе significant еffеct on impulsе buying, (2) storе еnvironmеnt has no significant еffеct on impulsе buying, (3) situational factors has significant еffеct on impulsе buying. From thе rеsult of this rеsеarch, thе managеrial of Hypеrmart Kеdiri Town Squarе еxpеctеd to carе about thе storе еnvironmеnt both in tеrm of storе planning, mеrchandising, storе dеsign and visual communication in ordеr to makе thе visitors influеncеd by thе stimuli from thе storе еnvironmеnt which makе thеm making impulsе buying. Kеywords : storе еnvironmеnt, situational factors, impulsе buying АBSTRАK Pеnеlitian ini mеnggunakan jеnis pеnеlitian еxplanatory rеsеarch dеngan pеndеkatan kuantitatif. Sampеl yang digunakan dalam pеnеlitian ini bеrjumlah 116 rеspondеn yang mеrupakan pеngunjung Hypеrmart Kеdiri Town Squarе. Mеtodе pеngumpulan data yang digunakan dalam pеnеlitian ini adalah kuеsionеr. Analisis data mеnggunakan analisis dеskriptif dan analisis rеgrеsi liniеr bеrganda. Hasil dari pеnеlitian ini mеnunjukkan bahwa (1) variabеl lingkungan toko dan faktor situasional sеcara simultan bеrpеngaruh signifikan tеrhadap impulsе buying. (2) variabеl lingkungan toko sеcara parsial bеrpеngaruh tidak signifikan tеrhadap impulsе buying. (3) variabеl faktor situasional sеcara parsial bеrpеngaruh signifikan tеrhadap impulsе buying. Dari hasil pеnеlitian ini manajеmеn dari Hypеrmart Kеdiri Town Squarе diharapkan lеbih mеmpеrhatikan lingkungan toko baik dari sеgi pеrеncanaan toko (alokasi ruang dan layout toko), dеsain toko (dеsain luar dan dеsain dalam toko), mеrchandising / pеnataan barang, dan juga komunikasi visual bеrupa postеr agar para pеngunjung tеrpеngaruh olеh stimulus yang didapat dari lingkungan toko sеhingga mеnimbulkan impulsе buying. Kаtа Kunci: Lingkungan toko, faktor situasional, impulsе buying

Video Profile Jurusan Sistem Komputer Jenjang Strata Satu Pada STMIK Raharja Tangerang

Currently, many formal and non formal education institutions are competing to get new students, so the profile of an educational institution becomes an important identity in introducing the institution to the general public. Various ways are used to socialize his profile, one of them STMIK Raharja Tangerang. In this modern era, multimedia plays an important role in the field of marketing as a promotion. The purpose of this study is, for prospective students / i new interested in the Department of Computer Systems, as well as a means of information and promotion effective and attractive to the general public. The problem is, the previous SK's promotional program only uses the brochure and banners media which information is not complete and interesting, for that SK Department requires video profile media capable of providing detailed information and interesting about the Department of Computer Systems, The research methods are: problem analysis, data, media design analysis and, media production concept (KPM). The results of his research in the form of video video profile Department of Computer Systems, which explains the profile of Computer Systems Department STMIK Raharja include CCIT and Computer System concentration, learning methods, excellence, and facilities. With the design of video media profile majors Computer Systems STMIK Raharja can be an attraction to increase interest in prospective new students in the Department, and become an informative and useful media for the general public

Analisa Pengaruh Tingkat Kecemasan dan Depresi Pasien dengan Keluarga (Caregiver) terhadap Kualitas Hidup Pasien Kanker di Malang

Background: Cancer patients' quality of life is influenced by many factors, such as physical, social, and psychological. Both the patient and the caregiver can experience anxiety and depression. This study aims to know the impact of anxiety and depression levels in both patients and their caregivers on the patients' quality of life.Methods: Descriptive analytic cross-sectional study at the 3rd and 4th stage of cancer patients from 2015-2019 in Poli Onkologi Terpadu Rumah Sakit Umum Daerah Dr. Saiful Anwar (RSSA) Malang. Respondents chosen by purposive sampling method (n = 50) assessed using the Hospital Anxiety and Depression Scale (HADS) for anxiety and depression. Meanwhile, the quality of life assessed by the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire-C30 (EORTC QLQ-C30). Data will be analyzed using multiple regression tests, which was continued by Pearson's correlation with p-value 0,279 dan p < 0,05).Conclusion: Anxiety and depression in patients and families did not affect the patients’ quality of life. Meanwhile, in this study, there was a correlation between anxiety and depression in cancer patients and their familiesKeywords: Anxiety, depression, patient, caregiver, cancer patients’ quality of lif

Nonlinearity of Mechanochemical Motions in Motor Proteins

The assumption of linear response of protein molecules to thermal noise or structural perturbations, such as ligand binding or detachment, is broadly used in the studies of protein dynamics. Conformational motions in proteins are traditionally analyzed in terms of normal modes and experimental data on thermal fluctuations in such macromolecules is also usually interpreted in terms of the excitation of normal modes. We have chosen two important protein motors - myosin V and kinesin KIF1A - and performed numerical investigations of their conformational relaxation properties within the coarse-grained elastic network approximation. We have found that the linearity assumption is deficient for ligand-induced conformational motions and can even be violated for characteristic thermal fluctuations. The deficiency is particularly pronounced in KIF1A where the normal mode description fails completely in describing functional mechanochemical motions. These results indicate that important assumptions of the theory of protein dynamics may need to be reconsidered. Neither a single normal mode, nor a superposition of such modes yield an approximation of strongly nonlinear dynamics.Comment: 10 pages, 6 figure

Allosteric Regulation of DNA Cleavage and Sequence-Specificity through Run-On Oligomerization

SummarySgrAI is a sequence specific DNA endonuclease that functions through an unusual enzymatic mechanism that is allosterically activated 200- to 500-fold by effector DNA, with a concomitant expansion of its DNA sequence specificity. Using single-particle transmission electron microscopy to reconstruct distinct populations of SgrAI oligomers, we show that in the presence of allosteric, activating DNA, the enzyme forms regular, repeating helical structures characterized by the addition of DNA-binding dimeric SgrAI subunits in a run-on manner. We also present the structure of oligomeric SgrAI at 8.6 Å resolution, demonstrating the conformational state of SgrAI in its activated form. Activated and oligomeric SgrAI displays key protein-protein interactions near the helix axis between its N termini, as well as allosteric protein-DNA interactions that are required for enzymatic activation. The hybrid approach reveals an unusual mechanism of enzyme activation that explains SgrAI’s oligomerization and allosteric behavior

On the conservation of the slow conformational dynamics within the amino acid kinase family: NAGK the paradigm

N-Acetyl-L-Glutamate Kinase (NAGK) is the structural paradigm for examining the catalytic mechanisms and dynamics of amino acid kinase family members. Given that the slow conformational dynamics of the NAGK (at the microseconds time scale or slower) may be rate-limiting, it is of importance to assess the mechanisms of the most cooperative modes of motion intrinsically accessible to this enzyme. Here, we present the results from normal mode analysis using an elastic network model representation, which shows that the conformational mechanisms for substrate binding by NAGK strongly correlate with the intrinsic dynamics of the enzyme in the unbound form. We further analyzed the potential mechanisms of allosteric signalling within NAGK using a Markov model for network communication. Comparative analysis of the dynamics of family members strongly suggests that the low-frequency modes of motion and the associated intramolecular couplings that establish signal transduction are highly conserved among family members, in support of the paradigm sequence→structure→dynamics→function © 2010 Marcos et al

On the origin of the Boson peak in globular proteins

We study the Boson Peak phenomenology experimentally observed in globular proteins by means of elastic network models. These models are suitable for an analytic treatment in the framework of Euclidean Random Matrix theory, whose predictions can be numerically tested on real proteins structures. We find that the emergence of the Boson Peak is strictly related to an intrinsic mechanical instability of the protein, in close similarity to what is thought to happen in glasses. The biological implications of this conclusion are also discussed by focusing on a representative case study.Comment: Proceedings of the X International Workshop on Disordered Systems, Molveno (2006

Forces During Bacteriophage DNA Packaging and Ejection

The conjunction of insights from structural biology, solution biochemistry, genetics and single molecule biophysics has provided a renewed impetus for the construction of quantitative models of biological processes. One area that has been a beneficiary of these experimental techniques is the study of viruses. In this paper we describe how the insights obtained from such experiments can be utilized to construct physical models of processes in the viral life cycle. We focus on dsDNA bacteriophages and show that the bending elasticity of DNA and its electrostatics in solution can be combined to determine the forces experienced during packaging and ejection of the viral genome. Furthermore, we quantitatively analyze the effect of fluid viscosity and capsid expansion on the forces experienced during packaging. Finally, we present a model for DNA ejection from bacteriophages based on the hypothesis that the energy stored in the tightly packed genome within the capsid leads to its forceful ejection. The predictions of our model can be tested through experiments in vitro where DNA ejection is inhibited by the application of external osmotic pressure

Motion and Flexibility in Human Cytochrome P450 Aromatase

The crystal structures of human placental aromatase in complex with the substrate androstenedione and exemestane have revealed an androgen-specific active site and the structural basis for higher order organization. However, X-ray structures do not provide accounts of movements due to short-range fluctuations, ligand binding and protein-protein association. In this work, we conduct normal mode analysis (NMA) revealing the intrinsic fluctuations of aromatase, deduce the internal modes in membrane-free and membrane-integrated monomers as well as the intermolecular modes in oligomers, and propose a quaternary organization for the endoplasmic reticulum (ER) membrane integration. Dynamics of the crystallographic oligomers from NMA is found to be in agreement with the isotropic thermal factors from the X-ray analysis. Calculations of the root mean square fluctuations of the C-alpha atoms from their equilibrium positions confirm that the rigid-core structure of aromatase is intrinsic regardless of the changes in steroid binding interactions, and that aromatase self-association does not deteriorate the rigidity of the catalytic cleft. Furthermore, NMA on membrane-integrated aromatase shows that the internal modes in all likelihood contribute to breathing of the active site access channel. The collective intermolecular hinge bending and twisting modes provide the flexibility in the quaternary association necessary for membrane integration of the aromatase oligomers. Taken together, fluctuations of the active site, the access channel, and the heme-proximal cavity, and a dynamic quaternary organization could all be essential components of the functional aromatase in its role as an ER membrane-embedded steroidogenic enzyme