GaBoDS: The Garching-Bonn Deep Survey -- I. Anatomy of galaxy clusters in the background of NGC 300

The Garching-Bonn Deep Survey (GaBoDS) is a virtual 12 square degree cosmic shear and cluster lensing survey, conducted with the [email protected] MPG/ESO telescope at La Silla. It consists of shallow, medium and deep random fields taken in R-band in subarcsecond seeing conditions at high galactic latitude. A substantial amount of the data was taken from the ESO archive, by means of a dedicated ASTROVIRTEL program. In the present work we describe the main characteristics and scientific goals of GaBoDS. Our strategy for mining the ESO data archive is introduced, and we comment on the Wide Field Imager data reduction as well. In the second half of the paper we report on clusters of galaxies found in the background of NGC 300, a random archival field. We use weak gravitational lensing and the red cluster sequence method for the selection of these objects. Two of the clusters found were previously known and already confirmed by spectroscopy. Based on the available data we show that there is significant evidence for substructure in one of the clusters, and an increasing fraction of blue galaxies towards larger cluster radii. Two other mass peaks detected by our weak lensing technique coincide with red clumps of galaxies. We estimate their redshifts and masses.Comment: 20 pages, 16 figures, gzipped. An online postscript version with higher quality figures (3.3 MBytes) can be downloaded from http://www.mpa-garching.mpg.de/~mischa/ngc300/ngc300.ps.gz . Submitted to A&

Mass, Light and Colour of the Cosmic Web in the Supercluster SCL2243-0935 (z=0.447)

Context: In 2.2m MPG-ESO/WFI data we discovered several mass peaks through weak lensing, forming a possible supercluster at redshift 0.45. Through multi-colour wide-field imaging with CFHT/Megaprime and INT/WFC we identify early-type galaxies and trace the supercluster network with them. Through EMMI/NTT multi-object spectroscopy we verify the initial shear-selected cluster candidates. Using weak lensing we obtain mass estimates for the supercluster centre and the filaments. Results: We identified the centre of the SCL2243-0935 supercluster, MACS J2243-0935, which was found independently by Ebeling et al. (2010). 13 more clusters or overdensities are embedded in a filamentary network, half of them are already spectroscopically confirmed. Three (5-15) Mpc filaments are detected, and we estimate the global size of SCL2243 to 45x15x50 Mpc, making it one of the largest superclusters known at intermediate redshifts. Weak lensing yields r_200=(2.06+/-0.13) Mpc and M_200=(1.54+/-0.29)x10^15 M_sun for MACS J2243 with M/L=428+/-82, very similar to results from size-richness cluster scaling relations. Integrating the weak lensing surface mass density over the supercluster network (defined by increased i-band luminosity or g-i colours), we find (1.53+/-1.01)x10^15 M_sun and M/L=305+/-201 for the three main filaments, consistant with theoretical predictions. The filaments' projected surface mass density is 0.007-0.012, corresponding to 10-100 times the critical density. The greatly varying density of the cosmic web is also reflected in the mean colour of galaxies. Conclusions: SCL2243 is significantly larger and much more richly structured than other known superclusters such as A901/902 or MS0302 studied with weak lensing before. It is a text-book supercluster with little contamination along the line of sight, making it a perfect sandbox for testing new techniques probing the cosmic web.Comment: 26 pages, 16 figures, accepted for publication Astronomy and Astrophysics. Minor corrections implemented as requested by the refere

Spectral Diversity Successfully Estimates the α-Diversity of Biocrust-Forming Lichens

Biocrusts, topsoil communities formed by mosses, lichens, liverworts, algae, and cyanobacteria, are a key biotic component of dryland ecosystems worldwide. Experiments carried out with lichen- and moss-dominated biocrusts indicate that climate change may dramatically reduce their cover and diversity. Therefore, the development of reproducible methods to monitor changes in biocrust diversity and abundance across multiple spatio-temporal scales is key for evaluating how climate change may impact biocrust communities and the myriad of ecosystem functions and services that rely on them. In this study, we collected lichen-dominated biocrust samples from a semi-arid ecosystem in central Spain. Their α-diversity was then evaluated using very high spatial resolution hyperspectral images (pixel size of 0.091 mm) measured in laboratory under controlled conditions. Support vector machines were used to map the biocrust composition. Traditional α-diversity metrics (i.e., species richness, Shannon’s, Simpson’s, and Pielou’s indices) were calculated using lichen fractional cover data derived from their classifications in the hyperspectral imagery. Spectral diversity was calculated at different wavelength ranges as the coefficient of variation of different regions of the reflectance spectra of lichens and as the standard deviation of the continuum removal algorithm (SD_CR). The accuracy of the classifications of the images obtained was close to 100%. The results showed the best coefficient of determination (r2 = 0.47) between SD_CR calculated at 680 nm and the α-diversity calculated as the Simpson’s index, which includes species richness and their evenness. These findings indicate that this spectral diversity index could be used to track spatio-temporal changes in lichen-dominated biocrust communities. Thus, they are the first step to monitor α-diversity of biocrust-forming lichens at the ecosystem and regional levels, a key task for any program aiming to evaluate changes in biodiversity and associated ecosystem services in drylands.The research has received funding from the European Union’s Horizon 2020 research and innovation 514 program under the Marie Sklodowska-Curie grant agreement no. 721995. F.T.M. acknowledges support from the European Research Council grant agreement no. 647038 (BIODESERT)

Cosmic shear from STIS Pure Parallels: I Data

Following the second HST servicing mission in 1997 when the STIS instrument was installed and the capability for parallel observations was enhanced, a substantial archive of non-proprietary parallel data has been accumulating. In this paper, we discuss the use of unfiltered STIS imaging data for a project that requires deep observations along as many independent lines-of-sight as possible. We have developed a technique to determine which datasets in the archive can safely be co-added together and have developed an iterative co-addition technique which enabled us to produce 498 high-quality, deep images. The principal motivation for this work is to measure the Cosmic Shear on small angular scales and a value derived from these data will be presented in a subsequent paper. A valuable by-product of this work is a set of high quality combined fields which can be used for other projects. The data are publicly available at http://www.stecf.org/projects/shear/Comment: 8 pages, 7 figures. accepted for publication in A&

Reduced occupancy of hedgehogs (Erinaceus europaeus) in rural England and Wales: the influence of habitat and an asymmetric intra-guild predator

Agricultural landscapes have become increasingly intensively managed resulting in population declines across a broad range of taxa, including insectivores such as the hedgehog (Erinaceus europaeus). Hedgehog declines have also been attributed to an increase in the abundance of badgers (Meles meles), an intra-guild predator. The status of hedgehogs across the rural landscape at large spatial scales is, however, unknown. In this study, we used footprint tracking tunnels to conduct the first national survey of rural hedgehog populations in England and Wales. Single and two-species occupancy modelling was used to quantify hedgehog occupancy in relation to habitat and predator covariates. Hedgehog occupancy was low (22% nationally), and significantly negatively related to badger sett density and positively related to the built environment. Hedgehogs were also absent from 71% of sites that had no badger setts, indicating that large areas of the rural landscape are not occupied by hedgehogs. Our results provide the first field based national survey of hedgehogs, providing a robust baseline for future monitoring. Furthermore, the combined effects of increasing badger abundance and intensive agriculture may have provided a perfect storm for hedgehogs in rural Britain, leading to worryingly low levels of occupancy over large spatial scales

ASXL2 is essential for haematopoiesis and acts as a haploinsufficient tumour suppressor in leukemia

Additional sex combs-like (ASXL) proteins are mammalian homologues of additional sex combs (Asx), a regulator of trithorax and polycomb function in Drosophila. While there has been great interest in ASXL1 due to its frequent mutation in leukemia, little is known about its paralog ASXL2, which is frequently mutated in acute myeloid leukemia patients bearing the RUNX1-RUNX1T1 (AML1-ETO) fusion. Here we report that ASXL2 is required for normal haematopoiesis with distinct, non-overlapping effects from ASXL1 and acts as a haploinsufficient tumour suppressor. While Asxl2 was required for normal haematopoietic stem cell self-renewal, Asxl2 loss promoted AML1-ETO leukemogenesis. Moreover, ASXL2 target genes strongly overlapped with those of RUNX1 and AML1-ETO and ASXL2 loss was associated with increased chromatin accessibility at putative enhancers of key leukemogenic loci. These data reveal that Asxl2 is a critical regulator of haematopoiesis and mediates transcriptional effects that promote leukemogenesis driven by AML1-ETO

Major Role for Amphotericin B–Flucytosine Combination in Severe Cryptococcosis

BACKGROUND: The Infectious Diseases Society of America published in 2000 practical guidelines for the management of cryptococcosis. However, treatment strategies have not been fully validated in the various clinical settings due to exclusion criteria during therapeutic trials. We assessed here the optimal therapeutic strategies for severe cryptococcosis using the observational prospective CryptoA/D study after analyzing routine clinical care of cryptococcosis in university or tertiary care hospitals. METHODOLOGY/PRINCIPAL FINDINGS: Patients were enrolled if at least one culture grew positive with Cryptococcus neoformans. Control of sterilization was warranted 2 weeks (Wk2) and 3 months (Mo3) after antifungal therapy onset. 208 HIV-positive or -negative adult patients were analyzed. Treatment failure (death or mycological failure) at Wk2 and Mo3 was the main outcome measured. Combination of amphotericin B+flucytosine (AMB+5FC) was the best regimen for induction therapy in patients with meningoencephalitis and in all patients with high fungal burden and abnormal neurology. In those patients, treatment failure at Wk2 was 26% in the AMB+5FC group vs. 56% with any other treatments (p<0.001). In patients treated with AMB+5FC, factors independently associated with Wk2 mycological failure were high serum antigen titer (OR [95%CI] = 4.43[1.21-16.23], p = 0.025) and abnormal brain imaging (OR = 3.89[1.23-12.31], p = 0.021) at baseline. Haematological malignancy (OR = 4.02[1.32-12.25], p = 0.015), abnormal neurology at baseline (OR = 2.71[1.10-6.69], p = 0.030) and prescription of 5FC for less than 14 days (OR = 3.30[1.12-9.70], p = 0.030) were independently associated with treatment failure at Mo3. CONCLUSION/SIGNIFICANCE: Our results support the conclusion that induction therapy with AMB+5FC for at least 14 days should be prescribed rather than any other induction treatments in all patients with high fungal burden at baseline regardless of their HIV serostatus and of the presence of proven meningoencephalitis