Purification and properties of an endoglucanase from Thermoascus aurantiacus

AbstractAn Endo-cellulase was purified to homogeneity using ammonium sulfate precipitation, ion exchange and size exclusion chromatography from newly isolated strain of Thermoascus aurantiacus RBB-1. The recovery and purification fold were 13.3% and 6.6, respectively, after size exclusion chromatography. The purified cellulase has a molecular mass (M) of 35kDa. Optimum temperature for the enzyme was found to be 70°C and stability was upto 80°C for 1h. Along with higher stability at 80°C, enzyme showed half lives of 192h and 144h at 50 and 70°C respectively. The purified cellulase was optimally active at pH 4.0 and was stable over a broad pH range of 3.0–7.0. The enzyme purified showed apparent Km and Vmax values of 37mg/ml and 82.6U/min/mg protein respectively with higher salt tolerance of 10% for 1h

Effect of Soil and Foliar Application of Zn and Fe on Nutrient Status of Hybrid Maize (Zea mays L.)

The present experiment was carried out to study the effect of soil and foliar application of zinc and iron on nutrient status of maize at Sardar Vallabhbhai University, Meerut during Kharif season of 2017-18. The present study consists of soil application of eight nutrient treatments namely 100% NPK, NPK+ Zn and Fe each @ 5 kg/ha,@ 10 kg/ha,@ 20 kg/ha, NPK+ Zn and Fe each @10 kg/ha + vermicompost (1.5 t/ha), NPK+Vermicompost @ 1.5 t/ha, NPK+ Zn and Fe each @ 5 kg/ha + foliar spray each @ 0.1% at the tasselling stage and NPK+ Foliar spray Zn and Fe each @ 0.1% at 25 DAS and at tasselling stage. These treatments were tested in RBD with three replications, aiming to know the effect of micro-nutrients (Zinc and Iron) on performance nutrient content and uptake of the maize cultivar, ADV 755. The maximum nitrogen, phosphorus & potassium content and uptake were recorded in the treatment number T5(T1 +soil applied Zn and Fe each @10 kg/ha + vermicompost), the maximum Zn and Fe content and uptake were recorded in the treatment number T7(T1+Soil applied Zn and Fe each @ 5 kg/ha + foliar spray each @ 0.1% at tasseling stage) wherein N,P,K, Zn and Fe were applied with vermicompost.The application of N,P,K and vermicompostwould have also helped in increasing N,P and K content and uptake in plants. The maximum Zn and Fe, content and uptake were recorded in Treatment T7which may have happened due to better soil and foliage application availability of nutrients in it

Effects of substrate binding site residue substitutions of xynA from Bacillus amyloliquefaciens on substrate specificity

Abstract Background The aromatic residues of xylanase enzyme, W187, Y124, W144, Y128 and W63 of substrate binding pocket from Bacillus amyloliquefaciens were investigated for their role in substrate binding by homology modelling and sequence analysis. These residues are highly conserved and play an important role in substrate binding through steric hindrance. The substitution of these residues with alanine allows the enzyme to accommodate nonspecific substrates. Results Wild type and mutated genes were cloned and overexpressed in BL21. Optimum pH and temperature of rBAxn exhibited pH 9.0 and 50 °C respectively and it was stable up to 215 h. Along with the physical properties of rBAxn, kinetic parameters (K m 19.34 ± 0.72 mg/ml; k cat 6449.12 ± 155.37 min− 1 and k cat /K m 333.83 ± 6.78 ml min− 1 mg− 1) were also compared with engineered enzymes. Out of five mutations, W63A, Y128A and W144A lost almost 90% activity and Y124A and W187A retained almost 40–45% xylanase activity. Conclusions The site-specific single mutation, led to alteration in substrate specificity from xylan to CMC while in case of double mutant the substrate specificity was altered from xylan to CMC, FP and avicel, indicating the role of aromatic residues on substrate binding, catalytic process and overall catalytic efficiency

Abstracts of National Conference on Research and Developments in Material Processing, Modelling and Characterization 2020

This book presents the abstracts of the papers presented to the Online National Conference on Research and Developments in Material Processing, Modelling and Characterization 2020 (RDMPMC-2020) held on 26th and 27th August 2020 organized by the Department of Metallurgical and Materials Science in Association with the Department of Production and Industrial Engineering, National Institute of Technology Jamshedpur, Jharkhand, India. Conference Title: National Conference on Research and Developments in Material Processing, Modelling and Characterization 2020Conference Acronym: RDMPMC-2020Conference Date: 26–27 August 2020Conference Location: Online (Virtual Mode)Conference Organizer: Department of Metallurgical and Materials Engineering, National Institute of Technology JamshedpurCo-organizer: Department of Production and Industrial Engineering, National Institute of Technology Jamshedpur, Jharkhand, IndiaConference Sponsor: TEQIP-

Open data from the first and second observing runs of Advanced LIGO and Advanced Virgo

Advanced LIGO and Advanced Virgo are monitoring the sky and collecting gravitational-wave strain data with sufficient sensitivity to detect signals routinely. In this paper we describe the data recorded by these instruments during their first and second observing runs. The main data products are gravitational-wave strain time series sampled at 16384 Hz. The datasets that include this strain measurement can be freely accessed through the Gravitational Wave Open Science Center at http://gw-openscience.org, together with data-quality information essential for the analysis of LIGO and Virgo data, documentation, tutorials, and supporting software