Actas de Horticultura

En un cultivo hidropónico recirculado de rosas cv. Dallas, se ha efectuado un seguimiento hora a hora de los consumos de agua y nitrato, con el objetivo de conocer mejor la cinética de absorción mineral por las plantas, con vistas a perfeccionar el manejo del sistema. Los resultados indican relaciones más o menos complejas con la radiación solar, temperaturas del aire y de la solución nutritiva, principalmente, así como con la tasa transpiratoria, con la que se registra relación lineal positiva durante las horas del día, pero no así por la noche. En las horas nocturnas la concentración de absorción de nitrato es más alta que de día

The effect of strontium incorporation into sol-gel biomaterials on their protein adsorption and cell interactions

[EN] It is known strontium can both inhibit the osteoclast formation and stimulate the osteoblast maturation, so biomaterials containing this element can favour bone structure stabilisation. The addition of Sr to biomaterials could affect their interactions with proteins and cells. Here, a silica-hybrid sol-gel network doped with different amounts of SrCl2 and applied as coatings on titanium discs was examined. in vitro analysis was performed to determine the potential effect of Sr in the coatings, showing enhanced gene expression of osteogenic markers (alkaline phosphatase and transforming growth factor-beta) in MC3T3-E1 incubated with Sr-doped biomaterials. The examination of inflammatory markers (tumour necrosis factor-alpha and interleukin 10) in RAW 264.7 macrophages revealed an anti-inflammatory potential of these materials. Proteins adsorbed onto the coatings incubated with human serum (3 h at 37 degrees C) were also analysed; mass spectrometry was used to characterise the proteins adhering to materials with different Sr content. Adding Sr to the coatings increased their affinity to APOE and VTNC proteins (associated with anti-inflammatory and osteogenic functions). Moreover, the proteins involved in coagulation processes, such as prothrombin, were more abundant on the coatings containing Sr than on the base sol-gel surfaces. Correlations between gene expression and proteomic results were also examined.This work was supported by MINECO (MAT2017-86043-R); Universitat Jaume I (grant numbers Predoc/2014/25, UJI-B2017-37); Basque Government (grant numbers IT611-13, Predoc/2016/1/0141), and University of the Basque Country (UFI11/56). Authors would like to thank Antonio Coso and Jaime Franco (GMI-Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver, Jose Ortega (UJI), and Iraide Escobes (CIC bioGUNE) for their valuable technical assistance.Romero-Gavilán, F.; Araújo-Gomes, N.; García-Arnáez, I.; Martínez-Ramos, C.; Elortza, F.; Azkargorta, M.; Iloro, I.... (2019). The effect of strontium incorporation into sol-gel biomaterials on their protein adsorption and cell interactions. Colloids and Surfaces B Biointerfaces. 174:9-16. https://doi.org/10.1016/j.colsurfb.2018.10.075S91617

Proteomic analysis of calcium-enriched sol-gel biomaterials

[EN] Calcium is an element widely used in the development of biomaterials for bone tissue engineering as it plays important roles in bone metabolism and blood coagulation. The Ca ions can condition the microenvironment at the tissue-material interface, affecting the protein deposition process and cell responses. The aim of this study was to analyze the changes in the patterns of protein adsorption on the silica hybrid biomaterials supplemented with different amounts of CaCl2, which can function as release vehicles. This characterization was carried out by incubating the Ca-biomaterials with human serum. LC-MS/MS analysis was used to characterize the adsorbed protein layers and compile a list of proteins whose affinity for the surfaces might depend on the CaCl2 content. The attachment of pro- and anti-clotting proteins, such as THRB, ANT3, and PROC, increased significantly on the Ca-materials. Similarly, VTNC and APOE, proteins directly involved on osteogenic processes, attached preferentially to these surfaces. To assess correlations with the proteomic data, these formulations were tested in vitro regarding their osteogenic and inflammatory potential, employing MC3T3-E1 and RAW 264.7 cell lines, respectively. The results confirmed a Ca dose-dependent osteogenic and inflammatory behavior of the materials employed, in accordance with the protein attachment patterns.This work was supported by MINECO [MAT2017-86043-R]; Universitat Jaume I [Grant numbers Predoc/2014/25, UJI-B2017-37]; Basque Government [Grant numbers IT611-13, Predoc/2016/1/0141]; University of the Basque Country [Grant number UFI11/56]. CIC bioGUNE is supported by Basque Department of Industry, Tourism and Trade (Etortek and Elkartek programs), the Innovation Technology Department of the Bizkaia County; The ProteoRed-ISCIII (Grant PRB3 IPT17/0019); CIBERehd Network, and Severo Ochoa Grant (SEV-2016-0644). Authors would like to thank Antonio Coso and Jaime Franco (GMI-Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver, Jose Ortega (UJI) and Iraide Escobes (CIC bioGUNE) for their valuable technical assistance.Romero-Gavilán, F.; Araújo-Gomes, N.; Cerqueira, A.; García-Arnáez, I.; Martínez-Ramos, C.; Azkargorta, M.; Iloro, I.... (2019). Proteomic analysis of calcium-enriched sol-gel biomaterials. JBIC Journal of Biological Inorganic Chemistry. 24(4):563-574. https://doi.org/10.1007/s00775-019-01662-5S563574244Berridge MJ, Bootman MD, Lipp P (1998) Calcium—a life and death signal. Nature 395:645–648. https://doi.org/10.1038/27094Hoppe A, Güldal NS, Boccaccini AR (2011) A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials 32:2757–2774. https://doi.org/10.1016/j.biomaterials.2011.01.004Flynn A (2003) The role of dietary calcium in bone health. 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J Biol Inorg Chem 23:459–470. https://doi.org/10.1007/s00775-018-1553-9Romero-Gavilán F, Sanchez-Pérez AM, Araújo-Gomes N et al (2017) Proteomic analysis of silica hybrid sol-gel coatings: a potential tool for predicting the biocompatibility of implants in vivo. Biofouling 33:676–689. https://doi.org/10.1080/08927014.2017.1356289Araújo-Gomes N, Romero-Gavilán F, Sanchez-Pérez AM et al (2018) Characterization of serum proteins attached to distinct sol-gel hybrid surfaces. J Biomed Mater Res Part B Appl Biomater 106:1477–1485. https://doi.org/10.1002/jbm.b.33954Romero-Gavilan F, Araújo-Gomes N, Sánchez-Pérez AM et al (2017) Bioactive potential of silica coatings and its effect on the adhesion of proteins to titanium implants. Colloids Surfaces B Biointerfaces 162:316–325. https://doi.org/10.1016/j.colsurfb.2017.11.072Shiu HT, Goss B, Lutton C et al (2014) Formation of blood clot on biomaterial implants influences bone healing. 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Signs of Overload After an Intensified Training

[EN] This study investigated eff ects of a 9-week intensified aerobic training and 3-weeks of recovery on signs of overload in 9 healthy active young males. Blood and saliva samples were collected and psychological questionnaires were administered during baseline (T1), intermediate load (T2), maximal load (T3), and recovery (T4) periods. Maximal oxygen uptake increased and blood lactate concentration decreased in T3, while running time in a 3 000 m track fi eld test was significantly shorter. No signifi cant changes were found in hematocrit, haemoglobin concentration, white blood cell count, lactate dehydrogenase, transaminases, interleukin-6, tumour necrosis factor- α , myeloperoxidase and markers of oxidative stress in plasma, or salivary cortisol and testosterone. Increases in diff erent negative aff ectscales and in the total mood disturbance score of the Profi le of Mood States were observed during T3. Scores in the stress scales of the Recovery- Stress Questionnaire for Athletes and in the State Anxiety Scale of the State-Trait Anxiety Inventory also showed signifi cant increases during T3. The lack of eff ects in biomarkers together with the changes observed in psychological assessment indicates that an intensifi ed training can produce psychological disturbances prone to early overreaching development. Additionally, it seems that psychological parameters are sensitive markers to detect stress produced by load increases.SIThis work was supported by the Acción Estratégica Sobre el Deporte, Spain (grants n ° 2006-56141-C03-01 to J. G., n ° 2006- 56141-C03-02 to S.M., and n ° 2006-56141-C03-03 to F.S.

Complement proteins regulating macrophage polarisation on biomaterials

[EN] One of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, distinct complement pathways can be activated to produce a foreign body reaction. In this article, we have designed different types of biomaterial surfaces to study the inflammation process. Here, we used different concentrations of (3-glycidoxypropyl)-trimethoxysilane (GPTMS), an organically-modified alkoxysilane as a precursor for the synthesis of various types of sol-gel materials functionalizing coatings for titanium implants to regulate biological responses. Our results showed that greater GPTMS surface concentrations induced greater secretion of TNF-alpha and IL-10 on RAW 264.7 macrophages. When implanted into rabbit tibia, osseointegration decreased with higher GPTMS concentrations. Interestingly, higher deposition of complement-related proteins C-reactive protein (CRP) and ficolin-2 (FCN2), two main activators of distinct complement pathways, was observed. Taking all together, inflammatory potential increase seems to be GPTMS concentration-dependent. Our results show that a greater adsorption of complement proteins can condition macrophage polarization.This work was supported by MINECO [MAT2017-86043-R]; Universitat Jaume I [Predoc/2014/25, UJI-B2017-37]; Basque Government [IT611-13, Predoc/2016/1/0141]; University of the Basque Country [UFI11/56]; CIC bioGUNE is supported by Basque Department of Industry, Tourism and Trade (Etortek and Elkartek programs), ProteoRed-ISCIII [PRB3 IPT17/0019]; CIBERehd Network and Severo Ochoa Grant [SEV-2016-0644]. Authors would like to thank Antonio Coso and Jaime Franco (GMI-Ilerimplant) for their inestimable contribution to this study, and Raquel Oliver, Jose Ortega (UJI), René van Rheden, Vicent Cuijpers (Radboudumc) and Iraide Escobes (CIC bioGUNE) for their valuable technical assistance.Araújo-Gomes, N.; Romero-Gavilán, F.; Zhang, Y.; Martínez-Ramos, C.; Elortza, F.; Azkargorta, M.; Martín De Llano, JJ.... (2019). Complement proteins regulating macrophage polarisation on biomaterials. Colloids and Surfaces B Biointerfaces. 181:125-133. https://doi.org/10.1016/j.colsurfb.2019.05.039S12513318

Expected Performance of the ATLAS Experiment - Detector, Trigger and Physics

A detailed study is presented of the expected performance of the ATLAS detector. The reconstruction of tracks, leptons, photons, missing energy and jets is investigated, together with the performance of b-tagging and the trigger. The physics potential for a variety of interesting physics processes, within the Standard Model and beyond, is examined. The study comprises a series of notes based on simulations of the detector and physics processes, with particular emphasis given to the data expected from the first years of operation of the LHC at CERN

Testosterone and Cortisol Release among Spanish Soccer Fans Watching the 2010 World Cup Final

This field study investigated the release of testosterone and cortisol of a vicarious winning experience in Spanish fans watching the finals between Spain and the Netherlands in the 2010 FIFA World Cup Soccer. Spanish fans (n = 50) watched the match with friends or family in a public place or at home and also participated in a control condition. Consistent with hypotheses, results revealed that testosterone and cortisol levels were higher when watching the match than on a control day. However, neither testosterone nor cortisol levels increased after the victory of the Spanish team. Moreover, the increase in testosterone secretion was not related to participants' sex, age or soccer fandom, but the increase in total cortisol secretion during the match was higher among men than among women and among fans that were younger. Also, increases in cortisol secretion were greater to the degree that people were a stronger fan of soccer. Level of fandom further appeared to account for the sex effect, but not for the age effect. Generally, the testosterone data from this study are in line with the challenge hypothesis, as testosterone levels of watchers increased to prepare their organism to defend or enhance their social status. The cortisol data from this study are in line with social self-preservation theory, as higher cortisol secretion among young and greater soccer fans suggests that especially they perceived that a negative outcome of the match would threaten their own social esteem

The Polycomb Protein and E3 Ubiquitin Ligase Ring1B Harbors an IRES in its Highly Conserved 5′ UTR

Ring1B is an essential member of the highly conserved Polycomb group proteins, which orchestrate developmental processes, cell growth and stem cell fate by modifying local chromatin structure. Ring1B was found to be the E3 ligase that monoubiquitinates histone H2A, which adds a new level of chromatin modification to Polycomb group proteins. Here we report that Ring1B belongs to the exclusive group of proteins that for their translation depend on a stable 5′ UTR sequence in their mRNA known as an Internal Ribosome Entry Site (IRES). In cell transfection assays the Ring1B IRES confers significantly higher expression levels of Ring1B than a Ring1B cDNA without the IRES. Also, dual luciferase assays show strong activity of the Ring1B IRES. Although our findings indicate Ring1B can be translated under conditions where cap-dependent translation is impaired, we found the Ring1B IRES to be cap-dependent. This raises the possibility that translational control of Ring1B is a multi-layered process and that translation of Ring1B needs to be maintained under varying conditions, which is in line with its essential role as an E3 ligase for monoubiquitination of histone H2A in the PRC1 Polycomb protein complex