Agroforestry as an alternative management system to strengthen crop sustainability

Funding Information: This work was supported by national funds of Camões – Instituto da Cooperação e da Língua (Portugal), Agência Brasileira de Cooperação (Brazil), and Parque Nacional da Gorongosa (Mozambique), through the project GorongosaCafé (TriCafé), as well as through Portuguese national funds from Fundação para a Ciência e a Tecnologia, I.P. (FCT), through the grants SFRH/BD/135357/2017 (C. Cassamo), and the Scientific Employment Stimulus - Individual Call (CEEC Individual) - 2021.01107.CEECIND/CP1689/CT0001 (I. Marques), and to the research units UIDB/00239/2020 (CEF), UIDB/00329/2020 (CE3C) and UIDP/04035/2020 (GeoBioTec). Funding from CNPq, Brazil (F. Partelli) is also greatly acknowledged. Funding Information: This work was supported by national funds of Camões – Instituto da Cooperação e da Língua (Portugal), Agência Brasileira de Cooperação (Brazil), and Parque Nacional da Gorongosa (Mozambique), through the project GorongosaCafé (TriCafé), as well as through Portuguese national funds from Fundação para a Ciência e a Tecnologia , I.P. (FCT), through the grants SFRH/BD/135357/2017 (C. Cassamo), and the Scientific Employment Stimulus - Individual Call (CEEC Individual) - 2021.01107.CEECIND/CP1689/CT0001 (I. Marques), and to the research units UIDB/00239/2020 (CEF), UIDB/00329/2020 (CE3C) and UIDP/04035/2020 (GeoBioTec). Funding from CNPq , Brazil (F. Partelli) is also greatly acknowledged. Publisher Copyright: © 2023 The AuthorsClimate changes (CC) are a main global phenomenon, with a worldwide impact on natural and agricultural ecosystems. The objective of this study was to analyse the potential impact of future CC on the suitability of areas for rainfed coffee growth, both at the Mozambique national scale and in the Gorongosa Mountain, under Agroforestry (AFS) and Full Sun (FS) management systems. The latter study site is part of the Gorongosa National Park (PNG), one of the most biodiverse places and an outstanding case of successful ecosystem restoration, including the rainforest from Gorongosa Mountain. Additionally, coffee cultivation in PNG under AFS is part of a strategy to strengthen the socio-economic sustainability of the local population, and the recovery of biodiversity in a degraded tropical rainforest ecosystem. Future climate assessments were elaborated through bioclimatic and biophysical variables (Elevation), with Coffea arabica L. being modeled under the current conditions and four global climate models (GCMs) using four Shared Socio-economic Pathways (SSPs). Isothermality, annual precipitation, and altitude were the most important variables influencing suitable areas in Mozambique. The analysis revealed that currently suitable areas where C. arabica is grown in Mozambique will be negatively affected under future scenarios (SSP126 to SSP585) in both systems (AFS and FS), although with clear worst impacts for FS. Under AFS, suitable areas will be reduced between about half and two-thirds by 2041–2060, and up to 91% by 2081–2100 (depending on scenarios) at the whole country level. Additionally, in Gorongosa Mountain, almost all scenarios point to a 30% reduction of the suitable area by 2041–2060, reaching 50% by 2081–2100, both in SSP126 and SSP245 scenarios. In sharp contrast, at the whole country level, the FS system is projected to be unsuitable for most of Mozambique, with area losses close to or above two-thirds already in 2021–2040, and greater than 80% by 2061–2080. Under this system, the projections were even more dramatic, pointing to a total absence of adequate areas at Gorongosa Mountain already by 2021–2040. Overall, our study provides clear evidence that the implementation of AFS greatly reduces CC deleterious impacts, being crucial to guarantee the sustainability of the coffee crop in the near future.publishersversionpublishe

Impact of climate changes in the suitable areas for Coffea arabica L. production in Mozambique: Agroforestry as an alternative management system to strengthen crop sustainability

Climate changes (CC) are a main global phenomenon, with a worldwide impact on natural and agricultural ecosystems. The objective of this study was to analyse the potential impact of future CC on the suitability of areas for rainfed coffee growth, both at the Mozambique national scale and in the Gorongosa Mountain, under Agroforestry (AFS) and Full Sun (FS) management systems. The latter study site is part of the Gorongosa National Park (PNG), one of the most biodiverse places and an outstanding case of successful ecosystem restoration, including the rainforest from Gorongosa Mountain. Additionally, coffee cultivation in PNG under AFS is part of a strategy to strengthen the socio-economic sustainability of the local population, and the recovery of biodiversity in a degraded tropical rainforest ecosystem. Future climate assessments were elaborated through bioclimatic and biophysical variables (Elevation), with Coffea arabica L. being modeled under the current conditions and four global climate models (GCMs) using four Shared Socio-economic Pathways (SSPs). Isothermality, annual precipitation, and altitude were the most important variables influencing suitable areas in Mozambique. The analysis revealed that currently suitable areas where C. arabica is grown in Mozambique will be negatively affected under future scenarios (SSP126 to SSP585) in both systems (AFS and FS), although with clear worst impacts for FS. Under AFS, suitable areas will be reduced between about half and two-thirds by 2041–2060, and up to 91% by 2081–2100 (depending on scenarios) at the whole country level. Additionally, in Gorongosa Mountain, almost all scenarios point to a 30% reduction of the suitable area by 2041–2060, reaching 50% by 2081–2100, both in SSP126 and SSP245 scenarios. In sharp contrast, at the whole country level, the FS system is projected to be unsuitable for most of Mozambique, with area losses close to or above two-thirds already in 2021–2040, and greater than 80% by 2061–2080. Under this system, the projections were even more dramatic, pointing to a total absence of adequate areas at Gorongosa Mountain already by 2021–2040. Overall, our study provides clear evidence that the implementation of AFS greatly reduces CC deleterious impacts, being crucial to guarantee the sustainability of the coffee crop in the near future.info:eu-repo/semantics/publishedVersio

Endothelial Neuropilin Disruption in Mice Causes DiGeorge Syndrome-Like Malformations via Mechanisms Distinct to Those Caused by Loss of Tbx1

The spectrum of human congenital malformations known as DiGeorge syndrome (DGS) is replicated in mice by mutation of Tbx1. Vegfa has been proposed as a modifier of DGS, based in part on the occurrence of comparable phenotypes in Tbx1 and Vegfa mutant mice. Many additional genes have been shown to cause DGS-like phenotypes in mice when mutated; these generally intersect in some manner with Tbx1, and therefore impact the same developmental processes in which Tbx1 itself is involved. In this study, using Tie2Cre, we show that endothelial-specific mutation of the gene encoding the VEGFA coreceptor neuropilin-1 (Nrp1) also replicates the most prominent terminal phenotypes that typify DGS. However, the developmental etiologies of these defects are fundamentally different from those caused by absence of TBX1. In Tie2Cre/Nrp1 mutants, initial pharyngeal organization is normal but subsequent pharyngeal organ growth is impaired, second heart field differentiation is normal but cardiac outflow tract cushion organization is distorted, neural crest cell migration is normal, and palatal mesenchyme proliferation is impaired with no change in apoptosis. Our results demonstrate that impairment of VEGF-dependent endothelial pathways leads to a spectrum of DiGeorge syndrome-type malformations, through processes that are distinguishable from those controlled by Tbx1

Genomic variation in baboons from central Mozambique unveils complex evolutionary relationships with other Papio species

Background Gorongosa National Park in Mozambique hosts a large population of baboons, numbering over 200 troops. Gorongosa baboons have been tentatively identified as part of Papio ursinus on the basis of previous limited morphological analysis and a handful of mitochondrial DNA sequences. However, a recent morphological and morphometric analysis of Gorongosa baboons pinpointed the occurrence of several traits intermediate between P. ursinus and P. cynocephalus, leaving open the possibility of past and/or ongoing gene flow in the baboon population of Gorongosa National Park. In order to investigate the evolutionary history of baboons in Gorongosa, we generated high and low coverage whole genome sequence data of Gorongosa baboons and compared it to available Papio genomes. Results We confirmed that P. ursinus is the species closest to Gorongosa baboons. However, the Gorongosa baboon genomes share more derived alleles with P. cynocephalus than P. ursinus does, but no recent gene flow between P. ursinus and P. cynocephalus was detected when available Papio genomes were analyzed. Our results, based on the analysis of autosomal, mitochondrial and Y chromosome data, suggest complex, possibly male-biased, gene flow between Gorongosa baboons and P. cynocephalus, hinting to direct or indirect contributions from baboons belonging to the “northern” Papio clade, and signal the presence of population structure within P. ursinus. Conclusions The analysis of genome data generated from baboon samples collected in central Mozambique highlighted a complex set of evolutionary relationships with other baboons. Our results provided new insights in the population dynamics that have shaped baboon diversity.info:eu-repo/semantics/publishedVersio

The effect of Holistic Planned Grazing™ on African rangelands: a case study from Zimbabwe

Holistic Planned Grazing™ (HPG) is purported to have positive long-term effects on rangelands, enhancing ecosystem services. Given comparable environmental templates, but different management regimes, vegetation monitoring and landscape function analysis showed the Africa Center for Holistic Management (ACHM) at Dimbangombe had a significantly higher rangeland condition (composition, cover, standing crop and soil health) than adjacent Sizinda (SCR) and Monde (MCR) communal rangelands. Overall grazer density on ACHM is 42% higher than that of SCR (no data for MCR). Finer-scale satellite collar data for ACHM yielded a calculated stocking rate of 0,55 LSU ha−1 y−1 or 24 590 kg km−2, which constitutes high-density grazing. An energy flow estimate shows that the grazing resource would, on average, not be limiting for livestock on ACHM but limiting on SCR. HPG may include an element where kraals are inserted into degraded rangelands for a short period. Overall, ACHM shows stable perennial composition with smaller tufts significantly closer together. A similar result was visible in SCR where maize yields were visibly higher on kraaled areas than on adjacent untreated fields. HPG yields positive long-term effects on ecosystem services (soils and vegetation) and points to the HPG approach enhancing the sustainability of livestock and wildlife in this environment.Keywords: basalt, kraal, landscape function, perennial grasses, stocking densit

Ecological legacies of civil war: 35-year increase in savanna tree cover following wholesale large-mammal declines

1. Large mammalian herbivores (LMH) exert strong effects on plants in tropical savannas, and many wild LMH populations are declining. However, predicting the impacts of these declines on vegetation structure remains challenging. 2. Experiments suggest that tree cover can increase rapidly following LMH exclusion. Yet it is unclear whether these results scale up to predict ecosystem-level impacts of LMH declines, which often alter fire regimes, trigger compensatory responses of other herbivores and accompany anthropogenic land-use changes. Moreover, theory predicts that grazers and browsers should have opposing effects on tree cover, further complicating efforts to forecast the outcomes of community-wide declines. 3. We used the near-extirpation of grazing and browsing LMH from Gorongosa National Park during the Mozambican Civil War (1977–1992) as a natural experiment to test whether megafaunal collapse increased tree cover. We classified herbaceous and tree cover in satellite images taken (a) at the onset of war in 1977 and (b) in 2012, two decades after hostilities ceased. 4. Throughout the 3620-km2 park, proportional tree cover increased by 34% (from 0.29 to 0.39) – an addition of 362 km2 . Four of the park’s five major habitat zones (including miombo woodland, Acacia–Combretum–palm savanna, and floodplain grassland) showed even greater increases in tree cover (51–134%), with an average increase of 94% in ecologically critical Rift Valley habitats. Only in the eastern Cheringoma Plateau, which had historically low wildlife densities, did tree cover decrease (by 5%). 5. The most parsimonious explanation for these results is that reduced browsing pressure enhanced tree growth, survival and/or recruitment; we found no directional trends in rainfall or fire that could explain increased tree cover. 6. Synthesis. Catastrophic large-herbivore die-offs in Mozambique’s flagship national park were followed by 35 years of woodland expansion, most severely in areas where pre-war wildlife biomass was greatest. These findings suggest that browsing release supersedes grazer–grass–fire feedbacks in governing ecosystem-level tree cover, consistent with smaller-scale experimental results, although the potentially complementary effect of CO2 fertilization cannot be definitively ruled out. Future work in Gorongosa will reveal whether recovering LMH populations reverse this trend, or alternatively whether woody encroachment hinders ongoing restoration efforts

Data from: Ecological legacies of civil war: 35-year increase in savanna tree cover following wholesale large-mammal declines

1. Large mammalian herbivores (LMH) exert strong effects on plants in tropical savannas, and many wild LMH populations are declining. However, predicting the impacts of these declines on vegetation structure remains challenging. 2. Experiments suggest that tree cover can increase rapidly following LMH exclusion. Yet it is unclear whether these results scale up to predict ecosystem-level impacts of LMH declines, which often alter fire regimes, trigger compensatory responses of other herbivores, and accompany anthropogenic land-use changes. Moreover, theory predicts that grazers and browsers should have opposing effects on tree cover, further complicating efforts to forecast the outcomes of community-wide declines. 3. We used the near-extirpation of grazing and browsing LMH from Gorongosa National Park during the Mozambican Civil War (1977-1992) as a natural experiment to test whether megafaunal collapse increased tree cover. We classified herbaceous and tree cover in satellite images taken (a) at the onset of war in 1977 and (b) in 2012, two decades after hostilities ceased. 4. Throughout the 3620-km2 park, proportional tree cover increased by 34% (from 0.29 to 0.39)—an addition of 362 km2. Four of the park's five major habitat zones (including miombo woodland, Acacia-Combretum-palm savanna, and floodplain grassland) showed even greater increases in tree cover (51–134%), with an average increase of 94% in ecologically critical Rift Valley habitats. Only in the eastern Cheringoma Plateau, which had historically low wildlife densities, did tree cover decrease (by 5%). 5. The most parsimonious explanation for these results is that reduced browsing pressure enhanced tree growth, survival, and/or recruitment; we found no directional trends in rainfall or fire that could explain increased tree cover. 6. Synthesis: Catastrophic large-herbivore die-offs in Mozambique's flagship national park were followed by 35 years of woodland expansion, most severely in areas where pre-war wildlife biomass was greatest. These findings suggest that browsing release supersedes grazer-grass-fire feedbacks in governing ecosystem-level tree cover, consistent with smaller-scale experimental results, although the potentially complementary effect of CO2 fertilization cannot be definitively ruled out. Future work in Gorongosa will reveal whether recovering LMH populations reverse this trend, or alternatively whether woody encroachment hinders ongoing restoration efforts

Trypan blue not toxic for retinal pigment epithelium in vitro

PURPOSE: To investigate whether trypan blue has a toxic effect on cultured retinal pigment epithelial (retinal pigment epithelium) cells. DESIGN: Experimental study with a direct live/dead cell staining technique using fluorescent dyes. METHODS: Cultured human retinal pigment epithelium cells were exposed for 5 minutes to various concentrations of trypan blue (0.06%, 0.15%, 0.30%), and cell viability was confocally measured. RESULTS: No increased cell death was found in cultures incubated in any of the trypan blue concentrations used. CONCLUSION: These findings indicate that a short exposure of trypan blue does not have a toxic effect on cultured retinal pigment epithelium cells.status: publishe