Aktivitas Social Media Engagement pada Akun Instagram Conclave

Kegiatan marketing dianggap sebagai hubungan yang lebih kuat antara produsen dan konsumen, tidak hanya sekedar transaksi. Hal ini dibuktikan dengan adanya bentuk pemasaran social media engagement yang memanfaatkan media sosial sebagai wadah yang memungkinan terjadinya kontektivitas dan interaktivitas antaridividu dan kelompok. Oleh karena itu, tujuan dari praktik kerja magang ini adalah untuk mengetahui dan memahami aktivitas Conclave dalam membangun engagement dengan audiens melalui media sosial Instagram. Dalam pelaksanaan praktik kerja magang, social media management memiliki tanggung jawab mengelola akun Instagram Conclave. Praktik kerja magang ini dilakukan selama 60 hari kerja dan peserta mendapatkan softskill dan hardskill selama masa magang. Adapun sofkill yang didapatkan selama kerja magang antara lain kemampuan untuk beradaptasi dengan cepat, kemampuan bekerja bersama tim dengan baik, dan kemampuan dalam mengambil keputusan dengan cermat. Selain itu, perserta juga mendapatkan hardskill antara lain pemahaman mendalam dan pengaplikasian social media engagement dalam suatu bisnis atau perusahaan

Perancangan Product Profile Video Promosi dan Collateral Product dalam Upaya Membangun Brand Awareness Kopi Gunung Puntang

Kopi Gunung Puntang merupakan kopi single origin yang berasal dari Jawa Barat, kopi ini memiliki keunggulan berupa keunikan rasa dan aromanya yang harum. Kualitas kopi ini telah diakui internasional pada kejuaraan kopi di tahun 2016 oleh Speciality Coffee Association of America. Meskipun mendunia, nyatanya kopi ini belum dikenal di dalam negeri. Oleh sebab itu, karya ini bertujuan untuk membangun brand awareness dengan menggunakan video promosi dan collateral product untuk menghasilkan eksposur bagi kopi Gunung Puntang. Konten video dan collateral product dirancang secara konseptual melalui konsep di antaranya komunikasi pemasaran, sinematografi, desain grafis, dan brand awareness. Dengan aspek rasio 9:16 yang berorientasi vertikal, video promosi diproduksi untuk menyesuaikan karakteristik smartphone dan Instagram. Karya ini merancang empat video yang dibuat melalui tahap pra produksi, produksi serta pasca produksi. Video pertama berjudul Keajaiban dari Tanah Sunda dengan durasi empat menit dan ketiga video lain berduasi satu menit dengan tema "Gunung Puntang Experience". Karya ini juga merancang desain ilustrasi poster, flyer, dan rack display yang ketiganya juga melewati proses perencanaan desain yang konseptual. Total tujuh karya yang terdiri dari video dan collateral product dirancang sebagai upaya membangun awareness produk kopi gunung puntang, empat video diunggah di media sosial Instagram dengan hasil yang baik berdasarkan data insight. Akun @rkopipalalangon mengalami peningkatan berdasarkan indikator reach dan impression. Pembuat karya juga menyebar kuesioner yang berisi pertanyaan seputar penilaian karya yang dibagikan kepada 21 responden dengan hasil yang baik pada setiap karya yang dibuat

Cytogenetic complexity in chronic lymphocytic leukemia: definitions, associations and clinical impact

Recent evidence suggests that complex karyotype (CK) defined by the presence of 653 chromosomal aberrations (structural and/or numerical) identified by chromosome banding analysis (CBA) may be relevant for treatment decision-making in chronic lymphocytic leukemia (CLL). However, many challenges towards routine clinical application of CBA remain. In a retrospective study of 5290 patients with available CBA data, we explored both clinicobiological associations and the clinical impact of CK in CLL. We found that patients with 655 abnormalities, defined as high-CK, exhibit uniformly dismal clinical outcome, independently of clinical stage, TP53 aberrations (deletion of chromosome 17p and or TP53 mutations, TP53abs) and the expression of somatically hypermutated (M-CLL) or unmutated (U-CLL) immunoglobulin heavy variable genes (IGHV). Thus, they contrasted CK cases with 3 or 4 aberrations (low-CK and intermediate-CK, respectively) who followed aggressive disease courses only in the presence of TP53abs. At the other end of the spectrum, patients with CK and +12,+19 displayed an exceptionally indolent profile. Building upon CK, TP53abs and IGHV gene somatic hypermutation status, we propose a novel hierarchical model where patients with high-CK exhibit the worst prognosis, while M-CLL lacking CK or TP53abs as well as CK with +12,+19 show the longest overall survival. In conclusion, CK should not be axiomatically considered unfavorable in CLL, representing a heterogeneous group with variable clinical behavior. High-CK with 655 chromosomal aberrations emerges as prognostically adverse, independently of other biomarkers. Prospective clinical validation is warranted before finally incorporating high-CK in risk stratification of CLL

The holistic perspective of the INCISIVE project : artificial intelligence in screening mammography

Finding new ways to cost-effectively facilitate population screening and improve cancer diagnoses at an early stage supported by data-driven AI models provides unprecedented opportunities to reduce cancer related mortality. This work presents the INCISIVE project initiative towards enhancing AI solutions for health imaging by unifying, harmonizing, and securely sharing scattered cancer-related data to ensure large datasets which are critically needed to develop and evaluate trustworthy AI models. The adopted solutions of the INCISIVE project have been outlined in terms of data collection, harmonization, data sharing, and federated data storage in compliance with legal, ethical, and FAIR principles. Experiences and examples feature breast cancer data integration and mammography collection, indicating the current progress, challenges, and future directions

Different spectra of recurrent gene mutations in subsets of chronic lymphocytic leukemia harboring stereotyped B-cell receptors.

We report on markedly different frequencies of genetic lesions within subsets of chronic lymphocytic leukemia patients carrying mutated or unmutated stereotyped B-cell receptor immunoglobulins in the largest cohort (n=565) studied for this purpose. By combining data on recurrent gene mutations (BIRC3, MYD88, NOTCH1, SF3B1 and TP53) and cytogenetic aberrations, we reveal a subset-biased acquisition of gene mutations. More specifically, the frequency of NOTCH1 mutations was found to be enriched in subsets expressing unmutated immunoglobulin genes, i.e. #1, #6, #8 and #59 (22-34%), often in association with trisomy 12, and was significantly different (P<0.001) to the frequency observed in subset #2 (4%, aggressive disease, variable somatic hypermutation status) and subset #4 (1%, indolent disease, mutated immunoglobulin genes). Interestingly, subsets harboring a high frequency of NOTCH1 mutations were found to carry few (if any) SF3B1 mutations. This starkly contrasts with subsets #2 and #3 where, despite their immunogenetic differences, SF3B1 mutations occurred in 45% and 46% of cases, respectively. In addition, mutations within TP53, whilst enriched in subset #1 (16%), were rare in subsets #2 and #8 (both 2%), despite all being clinically aggressive. All subsets were negative for MYD88 mutations, whereas BIRC3 mutations were infrequent. Collectively, this striking bias and skewed distribution of mutations and cytogenetic aberrations within specific chronic lymphocytic leukemia subsets implies that the mechanisms underlying clinical aggressiveness are not uniform, but rather support the existence of distinct genetic pathways of clonal evolution governed by a particular stereotyped B-cell receptor selecting a certain molecular lesion(s

Disease-biased and shared characteristics of the immunoglobulin gene repertoires in marginal zone B cell lymphoproliferations.

The B cell receptor immunoglobulin (BcR IG) gene repertoires of marginal zone (MZ) lymphoproliferations were analyzed in order to obtain insight into their ontogenetic relationships. Our cohort included cases with MZ lymphomas (n=488) i.e. splenic (SMZL), nodal (NMZL) and extranodal (ENMZL) as well as provisional entities (n=76) according to the World Health Organization classification. The most striking IG gene repertoire skewing was observed in SMZL. However, restrictions were also identified in all other MZ lymphomas studied, particularly ENMZL, with significantly different IG gene distributions depending on the primary site of involvement. Cross-entity comparisons of the MZ IG sequence dataset with a large dataset of IG sequences (MZ-related or not; n=65,837) revealed four major clusters of cases sharing homologous ('public') heavy variable complementarity-determining region 3. These clusters included rearrangements from SMZL, ENMZL (gastric, salivary gland, ocular adnexa), chronic lymphocytic leukemia but also rheumatoid factors and non-malignant spleen MZ cells. In conclusion, different MZ lymphomas display biased immunogenetic signatures indicating distinct antigen exposure histories. The existence of rare public stereotypes raises the intriguing possibility that common, pathogen-triggered, immune-mediated mechanisms, may result in diverse B lymphoproliferations due to targeting versatile progenitor B cells and/or operating in particular microenvironments.This work was supported in part by H2020 “AEGLE, An analytics framework for integrated and personalized healthcare services in Europe”, by the European Union (EU); H2020 No. 692298 project “MEDGENET, Medical Genomics and Epigenomics Network” by the EU; grant AZV 15-30015A from the Ministry of Health of the Czech Republic, and the project CEITEC2020 LQ1601 from the Ministry of Education, Youth, and Sports of the Czech Republic; Bloodwise Research Grant (15019); the Swedish Cancer Society, the Swedish Research Council, the Knut and Alice Wallenberg Foundation, Karolinska Institutet, Stockholm, the Lion’s Cancer Research Foundation, Uppsala, the Marcus Borgström Foundation and Selander’s Foundation, Uppsala

Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347Funder: Flemish Society for Critical Care NursesAbstract: Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat

Correction to: Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

The original version of this article unfortunately contained a mistake

Prevalence, associated factors and outcomes of pressure injuries in adult intensive care unit patients: the DecubICUs study

Funder: European Society of Intensive Care Medicine; doi: http://dx.doi.org/10.13039/501100013347Funder: Flemish Society for Critical Care NursesAbstract: Purpose: Intensive care unit (ICU) patients are particularly susceptible to developing pressure injuries. Epidemiologic data is however unavailable. We aimed to provide an international picture of the extent of pressure injuries and factors associated with ICU-acquired pressure injuries in adult ICU patients. Methods: International 1-day point-prevalence study; follow-up for outcome assessment until hospital discharge (maximum 12 weeks). Factors associated with ICU-acquired pressure injury and hospital mortality were assessed by generalised linear mixed-effects regression analysis. Results: Data from 13,254 patients in 1117 ICUs (90 countries) revealed 6747 pressure injuries; 3997 (59.2%) were ICU-acquired. Overall prevalence was 26.6% (95% confidence interval [CI] 25.9–27.3). ICU-acquired prevalence was 16.2% (95% CI 15.6–16.8). Sacrum (37%) and heels (19.5%) were most affected. Factors independently associated with ICU-acquired pressure injuries were older age, male sex, being underweight, emergency surgery, higher Simplified Acute Physiology Score II, Braden score 3 days, comorbidities (chronic obstructive pulmonary disease, immunodeficiency), organ support (renal replacement, mechanical ventilation on ICU admission), and being in a low or lower-middle income-economy. Gradually increasing associations with mortality were identified for increasing severity of pressure injury: stage I (odds ratio [OR] 1.5; 95% CI 1.2–1.8), stage II (OR 1.6; 95% CI 1.4–1.9), and stage III or worse (OR 2.8; 95% CI 2.3–3.3). Conclusion: Pressure injuries are common in adult ICU patients. ICU-acquired pressure injuries are associated with mainly intrinsic factors and mortality. Optimal care standards, increased awareness, appropriate resource allocation, and further research into optimal prevention are pivotal to tackle this important patient safety threat

Immunogenetic analysis of the T cell receptor repertoire of cytotoxic T lymphocytes with high throughput sequence

Introduction: Mono-, oligo- and poly- clonal expansions of CD3+CD8+CD57+ T-LGL may be either idiopathic or develop within the clinical context of several conditions e.g. autoimmunity, viral infections, post-transplant and in hematologic malignancies. Whether this heterogeneity reflects a dynamic process of cytotoxic T-cell responses against auto- and exoantigens remains to be established. Aim: The limited analytical depth of Sanger-based analysis has hindered firm conclusions from being drawn regarding the pathogenesis of these entities. In order to overcome these limitations and obtain insight into the role of antigenic drive in T-LGL lymphoproliferations, we here exploited high-throughput next generation sequencing (NGS) to interrogate the TRB gene repertoire. In the era of high throughput sequencing, our perceptions about immune responses will be revised. However, the absence of standardized experimental procedure and dedicated bioinformatics pipeline, special designed for immunogenetics data doesn’t ensure the repeatability of our results. Material and Methods: Included in the study were (i) a father and a son with T-LGL leukemia, the first case of intra-family occurrence; a single blood sample from the father and 2 samples from the son spanning 5 years were analyzed; and, (ii) a patient with T-LGL leukemia of donor cell origin developing after allogeneic hematopoietic cell transplantation (allo-HCT) for Philadelphia-positive acute lymphoblastic leukemia: and (iii) 13 well characterized Chronic Idiopathic Neutropenia (CIN) cases. TRBV-TRBJ-TRBD rearrangements were amplified on gDNA or/and cDNA and subjected to paired end NGS, covering the CDR3 twice/sequence. To increase the consistency of results, raw NGS reads were analyzed by a purpose-built bioinformatics algorithm, performing: (i) quality filtering, (ii) merging of filtered in paired reads and (iii) quality filter of stitched sequences. Filtered-in sequences were submitted to IMGT/HighV-QUEST, and metadata was processed by an in-house dedicated bioinformatics pipeline. Results: Major findings in all cases included: (i) pronounced skewing of the TRBV repertoire; (ii) existence of more than one immunodominant clonotype; (iii) persisting clonotypes in different timepoints albeit with fluctuating frequencies (clonal drift); and, (iv) shared (‘public’)clonotypes between cases and the public databases, further suggest a limited number of antigens implicated in pathogenesis of T-LGL cases.Conclusions: The borders between polyclonal versus oligoclonal versus monoclonal T-LGL lymphoproliferations are not sharply demarcated, but rather the transition from a polyclonal cytotoxic response to the development of T-LGL leukemia is a gradual process. Repertoire restrictions, public clonotypes and clonal drift strongly indicate selection by restricted (perhaps also shared) antigens in T-LGL leukemia ontogeny and evolution.Εισαγωγή: Οι λεμφοϋπερπλασίες CD3+CD8+CD57+ μεγάλων Τ λεμφοκυττάρων με κοκκία (T-LGL) αναπτύσσονται σε ευρύ νοσολογικό ετερογενές φάσμα διαφόρων κλινικών οντοτήτων και χαρακτηρίζονται από επικάλυψη κλινικών συμπτωμάτων αλλά και εργαστηριακών ευρημάτων. Η ανοσογενετική ανάλυση των γονιδίων της β αλυσίδας του Τ κυτταρικού υποδοχέα των κυτταροτοξικών Τ λεμφοκυττάρων των οντοτήτων αυτών αναδεικνύει ενδείξεις περί αντιγονικής εμπλοκής στην οντογένεση και πιθανώς εξέλιξη της νόσου. Σκοπός: Οι εγγενείς αδυναμίες της αλληλούχησης χαμηλής κλίμακας δεν μας επέτρεπαν την εξαγωγή ασφαλών συμπερασμάτων σχετικά με την παθογένεια των οντοτήτων. Η εφαρμογή μεθόδων αλληλούχησης υψηλής απόδοσης προσφέρει μία πληρέστερη εικόνα του ρεπερτορίου του Τ κυτταρικού υποδοχέα που θα ανασκευάσει τις απόψεις περί της σύστασης των άνοσων αποκρίσεων. Παρόλα αυτά, η έλλειψη προτυποιημένης πειραματικής διαδικασίας αλλά και βιοπληροφορικής ανάλυσης ειδικής για ανοσογενετικά δεδομένα δεν εξασφαλίζουν την επαναληψιμότητα των αποτελεσμάτων μας και την οριστική εξαγωγή συμπερασμάτων. Υλικά και μέθοδοι: Στην παρούσα μελέτη επιχειρήθηκε η προτυποποίηση της πειραματικής προσέγγισης αλλά και της βιοπληροφορικής ανάλυσης ανοσογενετικών δεδομένων τα οποία προκύπτουν με μεθόδους αλληλούχησης επόμενης γενιάς. Το προτυποποιημένο πειραματικό πρωτόκολλο εφαρμόσθηκε στη συνέχεια σε δείγματα (i) πατέρα και υιού με οικογενή T-LGL λευχαιμία (ii) ενός ασθενή με Ph+ALL που ανέπτυξε T-LGL λευχαιμία από τα κύτταρα του δότη μετά από αλλογενή μεταμόσχευση αιμοποιητικών κυττάρων (allo-HCT) και σε (iii) σε ασθενείς με Χρόνια Ιδιοπαθή Ουδετεροπενία (CIN), προκειμένου να αναζητηθούν περαιτέρω ανοσογενετικές μοριακές ενδείξεις για το ρόλο της επιλογής από αντιγόνο στις T-LGL λεμφοϋπερπλασίες. Αναδιατάξεις TRBV-TRBD-TRDJ ενισχύθηκαν από γενωμικό DNA ή/και cDNA με το πρωτόκολλο Biomed-2. Τα προϊόντα PCR υποβλήθηκαν σε paired-end πρωτόκολλο NGS (MiSeq Illumina). Η επεξεργασία των αλληλουχιών πραγματοποιήθηκε με εξειδικευμένο αλγόριθμο βιοπληροφορικής που περιλάμβανε: (i) ποιοτικά φίλτρα, (ii) σύνθεση των paired-end αλληλουχιών, (iii) προετοιμασία των συντεθειμένων αλληλουχιών για το εργαλείο IMGT/HighV-QUEST, και (iv) ομαδοποίηση και ερμηνεία των αποτελεσμάτων.Αποτελέσματα: Η ανάλυση των δεδομένων και στις τρεις περιπτώσεις κλινικών οντοτήτων επιβεβαιώνει : (i.) την επιλεκτικότητα του ρεπερτορίου, (ii.) την παρουσία δημόσιων κλωνότυπων και (iii.) τη διαχρονική παραμονή των εκπτυγμένων κλωνότυπων, δεδομένα που υπαινίσσονται ισχυρά επιλογή από περιορισμένο αριθμό αντιγόνων στην ανάπτυξη και πιθανώς εξέλιξη της T-LGL ανά περίπτωση. Συμπεράσματα: Τα όρια μεταξύ πολύ-, όλιγο-, μονο-, κλωνικότητας δεν είναι σαφώς οριοθετημένα. Η μετάβαση από μία πολυκλωνική κατάσταση σε μονοκλωνική εξαλλαγή των κυτταροτοξικών Τ λεμφοκυττάρων είναι μία δυναμική, πολυπαραγοντική διαδικασία, με το μικροπεριβάλλον να διαδραματίζει βασικό ρόλο. Η ανοσογενετική μελέτη των κυτταροτοξικών Τ λεμφοκυττάρων με μεθόδους αλληλούχησης υψηλής απόδοσης θα οδηγήσει στην αναθεώρηση των απόψεων περί σύστασης των άνοσων αποκρίσεων και θα προσφέρει ισχυρές ενδείξεις για την εμπλοκή αντιγόνου στη λεμφωματογένεση. Η φύση των αντιγόνων αυτών παραμένει να διευκρινιστεί