525 research outputs found
Observation of tropospheric clouds, dust aerosols and polar stratospheric clouds using thermal infrared spectra from TANSO-FTS/GOSAT
学位の種別: 課程博士審査委員会委員 : (主査)東京大学准教授 今須 良一, 東京大学教授 須貝 俊彦, 東京大学教授 高橋 正明, 東京大学准教授 芳村 圭, 情報通信研究機構主任研究員 笠井 康子University of Tokyo(東京大学
Gain in a quantum wire laser of high uniformity
A multi-quantum wire laser operating in the 1-D ground state has been
achieved in a very high uniformity structure that shows free exciton emission
with unprecedented narrow width and low lasing threshold. Under optical pumping
the spontaneous emission evolves from a sharp free exciton peak to a
red-shifted broad band. The lasing photon energy occurs about 5 meV below the
free exciton. The observed shift excludes free excitons in lasing and our
results show that Coulomb interactions in the 1-D electron-hole system shift
the spontaneous emission and play significant roles in laser gain.Comment: 4 pages, 4 figures, prepared by RevTe
Gas phase thermometry of hot turbulent jets using laser induced phosphorescence
This article is made available through the Brunel Open Access Publishing Fund. Copyright @ 2013 OSAThe temperature distributions of heated turbulent jets of air were determined using two dimensional (planar) laser induced phosphorescence. The jets were heated to specific temperature increments, ranging from 300 – 850 K and several Reynolds numbers were investigated at each temperature. The spectral ratio technique was used in conjunction with thermographic phosphors BAM and YAG:Dy, individually. Single shot and time averaged results are presented as two dimensional stacked images of turbulent jets. YAG:Dy did not produce a high enough signal for single shot measurements. The results allowed for a direct comparison between BAM and YAG:Dy, revealing that BAM is more suitable for relatively lower temperature, fast and turbulent regimes and that YAG:Dy is more suited to relatively higher temperature, steady flow situations
Photo-excitation band-structure engineering of 2H-NbSe probed by time- and angle-resolved photoemission spectroscopy
We investigated the nonequilibrium electronic structure of 2H-NbSe by
time- and angle-resolved photoemission spectroscopy. We find that the band
structure is distinctively modulated by strong photo-excitation, as indicated
by the unusual increase in the photoelectron intensities around E. In order
to gain insight into the observed photo-induced electronic state, we performed
DFT calculations with modulated lattice structures, and found that the
variation of the Se height from the Nb layer results in a significant change in
the effective mass and band gap energy. We further study the momentum-dependent
carrier dynamics. The results suggest that the relaxation is faster at the
K-centered Fermi surface than at the -centered Fermi surface, which can
be attributed to the stronger electron-lattice coupling at the K-centered Fermi
surface. Our demonstration of band structure engineering suggests a new role
for light as a tool for controlling the functionalities of solid-state
materials.Comment: 7 pages, 5 figure
Effectiveness and limitations of parameter tuning in reducing biases of top-of-atmosphere radiation and clouds in MIROC version 5
This study discusses how much of the biases in top-of-atmosphere (TOA) radiation and clouds can be removed by parameter tuning in the present-day simulation of a climate model in the Coupled Model Inter-comparison Project phase 5 (CMIP5) generation. We used output of a perturbed parameter ensemble (PPE) experiment conducted with an atmosphere–ocean general circulation model (AOGCM) without flux adjustment. The Model for Interdisciplinary Research on Climate version 5 (MIROC5) was used for the PPE experiment. Output of the PPE was compared with satellite observation data to evaluate the model biases and the parametric uncertainty of the biases with respect to TOA radiation and clouds. The results indicate that removing or changing the sign of the biases by parameter tuning alone is difficult. In particular, the cooling bias of the shortwave cloud radiative effect at low latitudes could not be removed, neither in the zonal mean nor at each latitude–longitude grid point. The bias was related to the overestimation of both cloud amount and cloud optical thickness, which could not be removed by the parameter tuning either. However, they could be alleviated by tuning parameters such as the maximum cumulus updraft velocity at the cloud base. On the other hand, the bias of the shortwave cloud radiative effect in the Arctic was sensitive to parameter tuning. It could be removed by tuning such parameters as albedo of ice and snow both in the zonal mean and at each grid point. The obtained results illustrate the benefit of PPE experiments which provide useful information regarding effectiveness and limitations of parameter tuning. Implementing a shallow convection parameterization is suggested as a potential measure to alleviate the biases in radiation and clouds
Down-Regulation of GEP100 Causes Increase in E-Cadherin Levels and Inhibits Pancreatic Cancer Cell Invasion
AIMS: Invasion and metastasis are major reasons for pancreatic cancer death and identifying signaling molecules that are specifically used in tumor invasion is of great significance. The purpose of this study was to elucidate the role of GEP100 in pancreatic cancer cell invasion and metastasis and the corresponding molecular mechanism. METHODS: Stable cell lines with GEP100 knocked-down were established by transfecting GEP100 shRNA vector into PaTu8988 cells and selected by puromycin. qRT-PCR and Western blot were performed to detect gene expression. Matrigel-invasion assay was used to detect cancer cell invasion in vitro. Liver metastasis in vivo was determined by splenic injection of indicated cell lines followed by spleen resection. Immunofluorescence study was used to detect the intracellular localization of E-cadherin. RESULTS: We found that the expression level of GEP100 protein was closely related to the invasive ability of a panel of 6 different human pancreatic cancer cell lines. Down-regulation of GEP100 in PaTu8988 cells significantly decreased invasive activity by Matrigel invasion assay, without affecting migration, invasion and viability. The inhibited invasive activity was rescued by over-expression of GEP100 cDNA. In vivo study showed that liver metastasis was significantly decreased in the PaTu8988 cells with GEP100 stably knocked-down. In addition, an epithelial-like morphological change, mimicking a mesenchymal to epithelial transition (MET) was induced by GEP100 down-regulation. The expression of E-cadherin protein was increased 2-3 folds accompanied by its redistribution to the cell-cell contacts, while no obvious changes were observed for E-cadherin mRNA. Unexpectedly, the mRNA of Slug was increased by GEP100 knock-down. CONCLUSION: These findings provided important evidence that GEP100 plays a significant role in pancreatic cancer invasion through regulating the expression of E-cadherin and the process of MET, indicating the possibility of it becoming a potential therapeutic target against pancreatic cancer
Update on the GOSAT TANSO–FTS SWIR Level 2 retrieval algorithm
The National Institute for Environmental Studies has provided the column-averaged dry-air mole fraction of carbon dioxide and methane (XCO and XCH) products (L2 products) obtained from the Greenhouse gases Observing SATellite (GOSAT) for more than a decade. Recently, we updated the retrieval algorithm used to produce the new L2 product, V03.00. The main changes from the previous version (V02) of the retrieval algorithm are the treatment of cirrus clouds, the degradation model of the Thermal And Near-infrared Spectrometer for carbon Observation–Fourier Transform Spectrometer (TANSO–FTS), solar irradiance spectra, and gas absorption coefficient tables. The retrieval results from the updated algorithm showed improvements in fitting accuracies in the O A, weak CO, and CH bands of TANSO–FTS, although the residuals increase in the strong CO band over the ocean. The direct comparison of the new product obtained from the updated (V03) algorithm with the previous version V02.90/91 and the validations using the Total Carbon Column Observing Network revealed that the V03 algorithm increases the amount of data without diminishing the data qualities of XCO and XCH over land. However, the negative bias of XCO is larger than that of the previous version over the ocean, and bias correction is still necessary. Additionally, the V03 algorithm resolves the underestimation of the XCO growth rate compared with the in situ measurements over the ocean recently found using V02.90/91 and V02.95/96
A distinct lineage of giant viruses brings a rhodopsin photosystem to unicellular marine predators.
Giant viruses are remarkable for their large genomes, often rivaling those of small bacteria, and for having genes thought exclusive to cellular life. Most isolated to date infect nonmarine protists, leaving their strategies and prevalence in marine environments largely unknown. Using eukaryotic single-cell metagenomics in the Pacific, we discovered a Mimiviridae lineage of giant viruses, which infects choanoflagellates, widespread protistan predators related to metazoans. The ChoanoVirus genomes are the largest yet from pelagic ecosystems, with 442 of 862 predicted proteins lacking known homologs. They are enriched in enzymes for modifying organic compounds, including degradation of chitin, an abundant polysaccharide in oceans, and they encode 3 divergent type-1 rhodopsins (VirR) with distinct evolutionary histories from those that capture sunlight in cellular organisms. One (VirRDTS) is similar to the only other putative rhodopsin from a virus (PgV) with a known host (a marine alga). Unlike the algal virus, ChoanoViruses encode the entire pigment biosynthesis pathway and cleavage enzyme for producing the required chromophore, retinal. We demonstrate that the rhodopsin shared by ChoanoViruses and PgV binds retinal and pumps protons. Moreover, our 1.65-Å resolved VirRDTS crystal structure and mutational analyses exposed differences from previously characterized type-1 rhodopsins, all of which come from cellular organisms. Multiple VirR types are present in metagenomes from across surface oceans, where they are correlated with and nearly as abundant as a canonical marker gene from Mimiviridae Our findings indicate that light-dependent energy transfer systems are likely common components of giant viruses of photosynthetic and phagotrophic unicellular marine eukaryotes
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