Clinical profile and treatment discontinuation in a tuberculosis control state programme in Brazil: preliminary results from SINAN database

Poster presented at the 45th ESCP Symposium on Clinical Pharmacy. 4-6 October 2016, Oslo, NorwayN/

Cure and discontinuation of treatment in a tuberculosis control state programme in Brazil: insights from dispensing data

Poster presented at the 45th ESCP Symposium on Clinical Pharmacy. 4-6 October 2016, Oslo, NorwayN/

Novel magnetic stimulation methodology for low-current implantable medical devices

Recent studies highlight the ability of inductive architectures to deliver therapeutic magnetic stimuli to target tissues and to be embedded into small-scale intracorporeal medical devices. However, to date, current micro-scale biomagnetic devices require very high electric current excitations (usually exceeding 1 A) to ensure the delivery of efficient magnetic flux densities. This is a critical problem as advanced implantable devices demand self-powering, stand-alone and long-term operation. This work provides, for the first time, a novel small-scale magnetic stimulation system that requires up to 50-fold lower electric current excitations than required by relevant biomagnetic technology recently proposed. Computational models were developed to analyse the magnetic stimuli distributions and densities delivered to cellular tissues during in vitro experiments, such that the feasibility of this novel stimulator can be firstly evaluated on cell culture tests. The results demonstrate that this new stimulative technology is able to deliver osteogenic stimuli (0.1-7 mT range) by current excitations in the 0.06-4.3 mA range. Moreover, it allows coil designs with heights lower than 1 mm without significant loss of magnetic stimuli capability. Finally, suitable core diameters and stimulator-stimulator distances allow to define heterogeneity or quasi-homogeneity stimuli distributions. These results support the design of high-sophisticated biomagnetic devices for a wide range of therapeutic applications.This work was funded by the Portuguese Foundation for Science and Technology (FCT), through the grant references SFRH/BPD/117475/2016, SFRH/BD/129340/2017 and IF/01089/2015, and by the European Structural and Investment Funds, through the project reference POCI-01-0145-FEDER-031132 and POCI-01-0145-FEDER-007679. It was also support by the TEMA - Centre for Mechanical Technology & Automation (UID/EMS/00481/2013-FCT and CENTRO-01-0145-FEDER-022083) and CICECO - Aveiro Institute of Materials (UID /CTM /50011/ 2013).in publicatio

Classificação fuzzy da vulnerabilidade aos processos costeiros em Pontal do Paraná, Brasil

The classification of the vulnerability for coastal processes considering both natural factors and anthropogenic interference can contribute for Integrated Coastal Zone Management (ICZM). This contribution develops a fuzzy system to classify the vulnerability of coastal processes in the coastal zone of Pontal do Sul, PR, Brazil. The study area was divided into three sectors with different characteristics, which provided diverse inputs to test this fuzzy system. Considered input variables are set as the follows: anthropogenic interference, average wave height, tidal range and the influence of the estuary in the region. The output of the final classification presents three linguistic labels, which represents three levels of vulnerability as stable, moderate and unstable. The results by the defuzzification in the fuzzy system are numerical values in a scale ranging from 0 to 8. The following values have been obtained as 5.9, 6.7 and 1.3; for the three sectors indicating unstable, unstable and stable classification respectively. In order to corroborate the efficacy of modeling, the results were compared with existing classification of the study area. The method can be an alternative approach for the expert’s role in decision-making in related studies.A classificação da vulnerabilidade aos processos costeiros considerando características tanto naturais quanto antrópicas serve de subsídio para a Gestão Integrada da Zona Costeira (GIZC). Nesta contribuição apresenta-se um sistema fuzzy para classificar a vulnerabilidade aos processos costeiros na região litorânea do Balneário de Pontal do Sul, PR, Brasil. A área de estudo foi dividida em três setores com características distintas, fornecendo diferentes valores para testar o sistema fuzzy. Este contém quatro variáveis de entrada: interferência antrópica, média de altura de ondas, amplitude de marés e a influência da desembocadura de um estuário. A classificação final do modelo apresenta como saída três variáveis linguísticas representando um determinado grau de vulnerabilidade, considerado como: estável, moderado e instável. Os resultados obtidos pelo sistema fuzzy, após a defuzzificação do sistema, são valores numéricos em uma escala de 0 a 8. Neste estudo, foram obtidos os seguintes valores: 5,9; 6,7; e 1,3 para os três setores avaliados, resultando numa classificação instável, instável e estável, respectivamente. Para corroborar a eficácia da modelagem, os resultados foram comparados com classificações existentes da área de estudo. Destaca-se que a metodologia é uma alternativa para contribuir com o papel do especialista e para tomada de decisão em estudos similares

Lathosterol oxidase (sterol C-5 desaturase) deletion confers resistance to amphotericin B and sensitivity to acidic stress in Leishmania major

Lathosterol oxidase (LSO) catalyzes the formation of the C-5-C-6 double bond in the synthesis of various types of sterols in mammals, fungi, plants, and protozoa. I

Differential Midgut Attachment of Leishmania (Viannia) braziliensis in the Sand Flies Lutzomyia (Nyssomyia) whitmani and Lutzomyia (Nyssomyia) intermedia

The interaction between Leishmania and sand flies has been demonstrated in many Old and New World species. Besides the morphological differentiation from procyclic to infective metacyclic promastigotes, the parasite undergoes biochemical transformations in its major surface lipophosphoglycan (LPG). An upregulation of β-glucose residues was previously shown in the LPG repeat units from procyclic to metacyclic phase in Leishmania (Viannia) braziliensis, which has not been reported in any Leishmania species. LPG has been implicated as an adhesion molecule that mediates the interaction with the midgut epithelium of the sand fly in the Subgenus Leishmania. These adaptations were explored for the first time in a species from the Subgenus Viannia, L. (V.) braziliensis with its natural vectors Lutzomyia (Nyssomyia) intermedia and Lutzomyia (Nyssomyia) whitmani. Using two in vitro binding techniques, phosphoglycans (PGs) derived from procyclic and metacyclic parasites were able to bind to the insect midgut and inhibit L. braziliensis attachment. Interestingly, L. braziliensis procyclic parasite attachment was ∼11-fold greater in the midgut of L. whitmani than in L. intermedia. The epidemiological relevance of L. whitmani as a vector of American Cutaneous Leishmaniasis (ACL) in Brazil is discussed

Development and application of a mechanistic nutrient-based model for precision fish farming

This manuscript describes and evaluates the FEEDNETICS model, a detailed mechanistic nutrient-based model that has been developed to be used as a data interpretation and decisionsupport tool by fish farmers, aquafeed producers, aquaculture consultants and researchers. The modelling framework comprises two main components: (i) fish model, that simulates at the individual level the fish growth, composition, and nutrient utilization, following basic physical principles and prior information on the organization and control of biochemical/metabolic processes; and (ii) farm model, that upscales all information to the population level. The model was calibrated and validated for five commercially relevant farmed fish species, i.e., gilthead seabream (Sparus aurata), European seabass (Dicentrarchus labrax), Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss), and Nile tilapia (Oreochromis niloticus), using data sets covering a wide range of rearing and feeding conditions. The results of the validation of the model for fish growth are consistent between species, presenting a mean absolute percentage error (MAPE) between 11.7 and 13.8%. Several uses cases are presented, illustrating how this tool can be used to complement experimental trial design and interpretation, and to evaluate nutritional and environmental effects at the farm level. FEEDNETICS provides a means of transforming data into useful information, thus contributing to more efficient fish farmingGrant agreement no. 818367; FEEDNETICS 4.0, funded by EUROSTARS-2 program; FEDER/ERDF, CRESC Algarve 2020 and NORTE 2020; PT-INNOVATION-0099; LA/P/0101/2020info:eu-repo/semantics/publishedVersio

Isolated familial somatotropinoma: 11q13-loh and gene/protein expression analysis suggests a possible involvement of aip also in non-pituitary tumorigenesis

OBJECTIVE: Non-pituitary tumors have been reported in a subset of patients harboring germline mutations in the aryl hydrocarbon receptor-interacting protein (AIP) gene. However, no detailed investigations of non-pituitary tumors of AIP-mutated patients have been reported so far. PATIENTS: We examined a MEN1- and p53-negative mother-daughter pair with acromegaly due to somatotropinoma. Subsequently, the mother developed a large virilizing adrenocortical carcinoma and a grade II B-cell non-Hodgkin's lymphoma. DESIGN: Mutational analysis was performed by automated sequencing. Loss-of-heterozygosity (LOH) analysis was carried out by sequencing and microsatellite analysis. AIP expression was assessed through quantitative PCR (qPCR) and immunohistochemistry. RESULTS: The functional inactivating mutation c.241C>T (R81X), which blocks the AIP protein from interacting with phosphodiesterase 4A (PDE4A), was identified in the heterozygous state in the leukocyte DNA of both patients. Analyzing the tumoral DNA revealed that the AIP wild-type allele was lost in the daughter's somatotropinoma and the mother's adrenocortical carcinoma. Both tumors displayed low AIP protein expression levels. Low AIP gene expression was confirmed by qPCR in the adrenocortical carcinoma. No evidence of LOH was observed in the DNA sample from the mother's B-cell lymphoma, and this tumor displayed normal AIP immunostaining. CONCLUSIONS: Our study presents the first molecular analysis of non-pituitary tumors in AIP-mutated patients. The finding of AIP inactivation in the adrenocortical tumor suggests that further investigation of the potential role of this recently identified tumor suppressor gene in non-pituitary tumors, mainly in those tumors in which the cAMP and the 11q13 locus are implicated, is likely to be worthwhile

Perfil da automedicação em município do Sul do Brasil

OBJETIVO: Caracterizar o usuário de medicamentos, especialmente aquele que se automedica. MATERIAL E MÉTODO: Foram entrevistadas 413 pessoas do Município de Santa Maria, Estado do Rio Grande do Sul, Brasil, sobre o consumo de medicamentos no último mês. RESULTADOS: Dos entrevistados, 69,9% utilizaram medicamentos e destes 76,1% o fizeram através de automedicação. Cefaléia (28,8%) foi a principal queixa motivadora de automedicação. O ácido acetilsalicílico foi a droga mais utilizada (25,4%). Dos fármacos utilizados na automedicação, 51,2% foram indicados por terceiros e 51,7% dessas indicações eram prescrições médicas emitidas em consultas anteriores. CONCLUSÃO: Idade, grau de escolaridade e acompanhamento médico periódico correlacionaram-se significativamente com automedicação.OBJECTIVE: The characterization of drug users, especially as regards self-medication and the determination of its prevalence in the population studied. MATERIAL AND METHOD: About 413 people that had used drugs in the previous month were interviewed. RESULTS: From the interviewed, 69.9% used medicines and of those 76.1% were self medicated. Headache (28.8%) was the main complaint among the self-medicated group. Acetilsalicilic acid was the most frequently used medicine (25.4%). As regards the drugs utilized, 51.2% of the users had received a recommendation from a third party and 51.7% used old prescriptions, given in previous consultations. CONCLUSION: Age, schooling and absence of periodic medical consultation were significant statistical factors in self-medication