412 research outputs found

    Writing in Translation: Robert Sullivan’s 'Star Waka' and Craig Santos Perez’s 'from unincorporated territory'

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    This article reads the multilingual poetics of Robert Sullivan's 'Star Waka' and Craig Santos Perez’s 'from unincorporated territory', showing how each poet deploys a range of formal, thematic, and imagistic strategies for expressing a contemporary transnationalism. Rather than identify a language of the metropole resisted by a threatened yet contestatory ‘local’ language, Sullivan and Perez cast apparently regional languages as equally traveled as the colonial languages that threaten to mask or silence them. In so doing, these poets argue not just for the vitality and resurgence of Maori and Chamorro respectively; they ultimately privilege neither ‘first’ nor ‘second’ language, neither ‘source’ nor ‘target,’ metropole nor colony, locating their argument for sovereignty in a kinetic space of translation, identifying the process of moving between heterogeneous languages which are irreducible to national literatures — even though they have been co-opted into nationalist discourses both oppressive and resistant — as equally valuable as the recourse to self-expression in an oppressed or minority language. This practice, which we term ‘writing in translation,’ offers evidence for a wider postcolonial turn, identified by critics such as Subramanian Shankar, Jacob Edmonds, and Gaytri Spivak, from seeing translation principally as evidence of colonial/imperial rupture and instead identifying within it a poetics of emergent discourse in which translation allows the multiple idioms and registers to co-exist, displaying a range of power structures and social hierarchies simultaneously

    Seeing Double? A Practice-Based Investigation into Twins experiences of Sporting Talent Development

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    While there is an established body of research on twins within the wider social science domain, scarce attention has been applied to this relationship within sport coaching practice. Specifically, this is apparent during talent development, despite a growing empirical interest toward the developmental impact of age-gapped siblings on sporting success. Accordingly, this study explored potential mechanisms through which the twin relationship may impact on talent development. Longitudinal observation of two twin sets (one monozygotic and one dizygotic) took place within a UK regional hockey performance centre training environment. Observations were used to inform semi-structured interviews with twins and their parents, which facilitated the interpretation of observations and exploration of the relationship, before a codebook thematic analysis was conducted. Findings revealed several themes (regularity of interaction, emotional interpersonal skills, rivalry, skill development, communication, and type of separation) consistent with previous studies, alongside two new themes; namely, conflict and identity. The study highlights the complex and individualized nature of the sibling subsystem, illuminating the possible impact of twin type on several themes, and highlights the potential for observations as a practice-based tool for coaches to consider when individualizing the talent development process

    Reduced Protein Expression of the Na+/Ca2++K+-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice

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    Exposure of forming enamel to fluoride results into formation of hypomineralized enamel. We tested whether enamel hypomineralization was caused by lower expression of the NCKX4/SLC24A4 Ca2+-transporter by ameloblasts. Three commercial antibodies against NCKX4 were tested on enamel organs of wild-type and Nckx4-null mice, one of which (a mouse monoclonal) was specific. This antibody gave a prominent staining of the apical plasma membranes of maturation ameloblasts, starting at early maturation. The layer of immuno-positive ameloblasts contained narrow gaps without immunostaining or with reduced staining. In fluorotic mouse incisors, the quantity of NCKX4 protein in ameloblasts as assessed by western blotting was not different from that in non-fluorotic ameloblasts. However, immunostaining of the apical plasma membranes of fluorotic ameloblasts was strongly reduced or absent suggesting that trafficking of NCKX4 to the apical membrane was strongly reduced. Exposure to fluoride may reduce NCKX4-mediated transport of Ca2+ by maturation stage ameloblasts which delays ameloblast modulation and reduces enamel mineralization

    Na+, K+-ATPase Subunit Composition in a Human Chondrocyte Cell Line; Evidence for the Presence of α1, α3, β1, β2 and β3 Isoforms

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    Membrane transport systems participate in fundamental activities such as cell cycle control, proliferation, survival, volume regulation, pH maintenance and regulation of extracellular matrix synthesis. Multiple isoforms of Na+, K+-ATPase are expressed in primary chondrocytes. Some of these isoforms have previously been reported to be expressed exclusively in electrically excitable cells (i.e., cardiomyocytes and neurons). Studying the distribution of Na+, K+-ATPase isoforms in chondrocytes makes it possible to document the diversity of isozyme pairing and to clarify issues concerning Na+, K+-ATPase isoform abundance and the physiological relevance of their expression. In this study, we investigated the expression of Na+, K+-ATPase in a human chondrocyte cell line (C-20/A4) using a combination of immunological and biochemical techniques. A panel of well-characterized antibodies revealed abundant expression of the α1, β1 and β2 isoforms. Western blot analysis of plasma membranes confirmed the above findings. Na+, K+-ATPase consists of multiple isozyme variants that endow chondrocytes with additional homeostatic control capabilities. In terms of Na+, K+-ATPase expression, the C-20/A4 cell line is phenotypically similar to primary and in situ chondrocytes. However, unlike freshly isolated chondrocytes, C-20/A4 cells are an easily accessible and convenient in vitro model for the study of Na+, K+-ATPase expression and regulation in chondrocytes

    Prefrontal Cortex Lesions Impair Object-Spatial Integration

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    How and where object and spatial information are perceptually integrated in the brain is a central question in visual cognition. Single-unit physiology, scalp EEG, and fMRI research suggests that the prefrontal cortex (PFC) is a critical locus for object-spatial integration. To test the causal participation of the PFC in an object-spatial integration network, we studied ten patients with unilateral PFC damage performing a lateralized object-spatial integration task. Consistent with single-unit and neuroimaging studies, we found that PFC lesions result in a significant behavioral impairment in object-spatial integration. Furthermore, by manipulating inter-hemispheric transfer of object-spatial information, we found that masking of visual transfer impairs performance in the contralesional visual field in the PFC patients. Our results provide the first evidence that the PFC plays a key, causal role in an object-spatial integration network. Patient performance is also discussed within the context of compensation by the non-lesioned PFC

    Surface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticles

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    This document is the Accepted Manuscript version of a Published Work that appeared in final form in Langmuir copyright © American Chemical Society after peer review and technical editing by publisher. To access the final edited and published work see Zhang, X., Servos, M. R., & Liu, J. (2012). Surface Science of DNA Adsorption onto Citrate-Capped Gold Nanoparticles. Langmuir, 28(8), 3896–3902. https://doi.org/10.1021/la205036pSingle-stranded DNA can be adsorbed by citrate capped gold nanoparticles (AuNPs), resulting in increased AuNP stability, which forms the basis of a number of biochemical and analytical applications, but the fundamental interaction of this adsorption reaction remains unclear. In this study, we measured DNA adsorption kinetics, capacity, and isotherms, demonstrating that the adsorption process is governed by electrostatic forces. The charge repulsion among DNA strands and between DNA and AuNPs can be reduced by adding salt, reducing pH or by using noncharged peptide nucleic acid (PNA). Langmuir adsorption isotherms are obtained, indicating the presence of both adsorption and desorption of DNA from AuNPs. While increasing salt concentration facilitates DNA adsorption, the desorption rate is also enhanced in higher salt due to DNA compaction. DNA adsorption capacity is determined by DNA oligomer length, DNA concentration, and salt. Previous studies indicated faster adsorption of short DNA oligomers by AuNPs, we find that once adsorbed, longer DNAs are much more effective in protecting AuNPs from aggregation. DNA adsorption is also facilitated by using low pH buffers and high alcohol concentrations. A model based on electrostatic repulsion on AuNPs is proposed to rationalize the DNA adsorption/desorption behavior.University of Waterloo || Canadian Foundation for Innovation || Ontario Ministry of Research & Innovation || Canadian Institutes of Health Research || Natural Sciences and Engineering Research Council |
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