Modelling the effect of density-dependent chemical interference upon seed germination

Peer reviewe

Effects of Contaminated Soil on the Survival and Growth Performance of European (Populus tremula L.) and Hybrid Aspen (Populus tremula L. × Populus tremuloides Michx.) Clones Based on Stand Density

This study was conducted to assess the survival rates, growth, and chlorophyll fluorescence (Fv/Fm) of four hybrid aspen (14, 191, 27, 291) and two European aspen (R3 and R4) clones cultivated in creosote- and diesel oil-contaminated soil treatments under three different plant densities: one plant per pot (low density), two plants per pot (medium density), and six plants per pot (high density) over a period of two years and three months. Evaluating the survival, growth, and Fv/Fm values of different plants is a prerequisite for phytoremediation to remediate polluted soils for ecological restoration and soil health. The results revealed that contaminated soils affected all plants’ survival rates and growth. However, plants grown in the creosote-contaminated soil displayed a 99% survival rate, whereas plants cultivated in the diesel-contaminated soil showed a 22–59% survival rate. Low plant density resulted in a higher survival rate and growth than in the other two density treatments. In contrast, the medium- and high-density treatments did not affect the plant survival rate and growth to a greater extent, particularly in contaminated soil treatments. The effects of clonal variation on the survival rate, growth, and Fv/Fm values were evident in all treatments. The results suggested that hybrid aspen clones 14 and 291, and European aspen clone R3 were suitable candidates for the phytoremediation experiment, as they demonstrated reasonable survival rates, growth, and Fv/Fm values across all treatments. A superior survival rate for clone 291, height and diameter growth, and stem dry biomass production for clone 14 were observed in all soil treatments. Overall, a reasonable survival rate (~75%) and Fv/Fm value (>0.75) for all plants in all treatments, indicating European aspen and hybrid aspen have considerable potential for phytoremediation experiments. As the experiment was set up for a limited period, this study deserves further research to verify the growth potential of different hybrid aspen and European aspen clones in different soil and density treatment for the effective phytoremediation process to remediate the contaminated soil

The abundance of health-associated bacteria is altered in PAH polluted soils-Implications for health in urban areas?

Long-term exposure to polyaromatic hydrocarbons (PAHs) has been connected to chronic human health disorders. It is also well-known that i) PAH contamination alters soil bacterial communities, ii) human microbiome is associated with environmental microbiome, and iii) alteration in the abundance of members in several bacterial phyla is associated with adverse or beneficial human health effects. We hypothesized that soil pollution by PAHs altered soil bacterial communities that had known associations with human health. The rationale behind our study was to increase understanding and potentially facilitate reconsidering factors that lead to health disorders in areas characterized by PAH contamination. Large containers filled with either spruce forest soil, pine forest soil, peat, or glacial sand were left to incubate or contaminated with creosote. Biological degradation of PAHs was monitored using GC-MS, and the bacterial community composition was analyzed using 454 pyrosequencing. Proteobacteria had higher and Actinobacteria and Bacteroidetes had lower relative abundance in creosote contaminated soils than in non-contaminated soils. Earlier studies have demonstrated that an increase in the abundance of Proteobacteria and decreased abundance of the phyla Actinobacteria and Bacteroidetes are particularly associated with adverse health outcomes and immunological disorders. Therefore, we propose that pollution-induced shifts in natural soil bacterial community, like in PAH-polluted areas, can contribute to the prevalence of chronic diseases. We encourage studies that simultaneously address the classic "adverse toxin effect" paradigm and our novel "altered environmental microbiome" hypothesis.Peer reviewe

Urbanization reduces transfer of diverse environmental microbiota indoors

Expanding urbanization is a major factor behind rapidly declining biodiversity. It has been proposed that in urbanized societies, the rarity of contact with diverse environmental microbiota negatively impacts immune function and ultimately increases the risk for allergies and other immune-mediated disorders. Surprisingly, the basic assumption that urbanization reduces exposure to environmental microbiota and its transfer indoors has rarely been examined. We investigated if the land use type around Finnish homes affects the diversity, richness, and abundance of bacterial communities indoors. Debris deposited on standardized doormats was collected in 30 rural and 26 urban households in and near the city of Lahti, Finland, in August 2015. Debris was weighed, bacterial community composition determined by high throughput sequencing of bacterial 16S ribosomal RNA (rRNA) gene on the Illumina MiSeq platform, and the percentage of four different land use types (i.e., built area, forest, transitional, and open area) within 200 m and 2000 m radiuses from each household was characterized. The quantity of doormat debris was inversely correlated with coverage of built area. The diversity of total bacterial, Proteobacterial, Actinobacterial, Bacteroidetes, and Firmicutes communities decreased as the percentage of built area increased. Their richness followed the same pattern except for Firmicutes for which no association was observed. The relative abundance of Proteobacteria and particularly Gammaproteobacteria increased, whereas that of Actinobacteria decreased with increasing built area. Neither Phylum Firmicutes nor Bacteroidetes varied with coverage of built area. Additionally, the relative abundance of potentially pathogenic bacterial families and genera increased as the percentage of built area increased. Interestingly, having domestic animals (including pets) only altered the association between the richness of Gammaproteobacteria and diversity of Firmicutes with the built area coverage suggesting that animal ownership minimally affects transfer of environmental microbiota indoors from the living environment. These results support the hypothesis that people living in densely built areas are less exposed to diverse environmental microbiota than people living in more sparsely built areas.Peer reviewe

GABAergic signaling as therapeutic target for autism spectrum disorders

gamma-Aminobutyric acid (GABA), the main inhibitory neurotransmitter in the adult brain, early in postnatal life exerts a depolarizing and excitatory action. This depends on accumulation of chloride inside the cell via the cation chloride importer NKCC1, being the expression of the chloride exporter KCC2 very low at birth. The developmentally regulated expression of KCC2 results in extrusion of chloride with age and a shift of GABA from the depolarizing to the hyperpolarizing direction. The depolarizing action of GABA leads to intracellular calcium rise through voltage-dependent calcium channels and/or N-methyl-D-aspartate receptors. GABA-mediated calcium signals regulate a variety of developmental processes from cell proliferation migration, differentiation, synapse maturation, and neuronal wiring. Therefore, it is not surprising that some forms of neuro-developmental disorders such as autism spectrum disorders (ASDs) are associated with alterations of GABAergic signaling and impairment of the excitatory/inhibitory balance in selective neuronal circuits. In this review, we will discuss how changes of GABAA-mediated neurotransmission affect several forms of ASDs including the Fragile X, the Angelman, and Rett syndromes. Then, we will describe various animal models of ASDs with GABAergic dysfunctions, highlighting their behavioral deficits and the possibility to rescue them by targeting selective components of the GABAergic synapse. In particular, we will discuss how in some cases, reverting the polarity of GABA responses from the depolarizing to the hyperpolarizing direction with the diuretic bumetanide, a selective blocker of NKCC1, may have beneficial effects on ASDs, thus opening new therapeutic perspectives for the treatment of these devastating disorders

Oxidovanadium(v) complexes with l-proline-based amino acid phenolates

L-proline was used to prepare chiral, tridentate amino acid phenol proligands H2L1—4. These proligands react with vanadium precursors VO(acac)2, VOSO4 ∙ 5 H2O and VO(OPr)3 in methanol to form the corresponding oxidoalkoxidovanadium(V) complexes 1—4. The complexes crystallize from methanol, and are octahedrally coordinated with a general formula [VO(L1—4)(OMe)(MeOH)]. In solution, however, they adopt several different conformations or isomeric structures depending on the solvent.peerReviewe

Effects of Contaminated Soil on the Survival and Growth Performance of European (Populus tremula L.) and Hybrid Aspen (Populus tremula L. × Populus tremuloides Michx.) Clones Based on Stand Density

This study was conducted to assess the survival rates, growth, and chlorophyll fluorescence (Fv/Fm) of four hybrid aspen (14, 191, 27, 291) and two European aspen (R3 and R4) clones cultivated in creosote- and diesel oil-contaminated soil treatments under three different plant densities: one plant per pot (low density), two plants per pot (medium density), and six plants per pot (high density) over a period of two years and three months. Evaluating the survival, growth, and Fv/Fm values of different plants is a prerequisite for phytoremediation to remediate polluted soils for ecological restoration and soil health. The results revealed that contaminated soils affected all plants’ survival rates and growth. However, plants grown in the creosote-contaminated soil displayed a 99% survival rate, whereas plants cultivated in the diesel-contaminated soil showed a 22–59% survival rate. Low plant density resulted in a higher survival rate and growth than in the other two density treatments. In contrast, the medium- and high-density treatments did not affect the plant survival rate and growth to a greater extent, particularly in contaminated soil treatments. The effects of clonal variation on the survival rate, growth, and Fv/Fm values were evident in all treatments. The results suggested that hybrid aspen clones 14 and 291, and European aspen clone R3 were suitable candidates for the phytoremediation experiment, as they demonstrated reasonable survival rates, growth, and Fv/Fm values across all treatments. A superior survival rate for clone 291, height and diameter growth, and stem dry biomass production for clone 14 were observed in all soil treatments. Overall, a reasonable survival rate (~75%) and Fv/Fm value (>0.75) for all plants in all treatments, indicating European aspen and hybrid aspen have considerable potential for phytoremediation experiments. As the experiment was set up for a limited period, this study deserves further research to verify the growth potential of different hybrid aspen and European aspen clones in different soil and density treatment for the effective phytoremediation process to remediate the contaminated soil

NMR metabolomics of ripened and developing oilseed rape (Brassica napus) and turnip rape (Brassica rapa)

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