The sustainability of small, medium and micro enterprises within the Nelson Mandela Bay Metropole

This research evaluates the sustainability of Small, Medium and Micro Enterprises (SMMEs) within the Nelson Mandela Bay Metropole. It seeks to identify whether the current government initiatives offered by the SEDA, DTI and CIDB are effectively addressing the major issues faced by SMMEs in order for them to remain sustainable. It seeks clarity on whether large construction companies should also take responsibility for keeping SMMEs sustainable. The challenges faced by SMMEs include limited financial resources, poor access to markets, little infrastructure, complex labour laws, rampant crime, shortage of skills and bureaucratic systems (SEDA 2016). Government has made concerted efforts to address these challenges so that affirmative action and Broad Based Black Economic Empowerment (B-BBEE) objectives are met. This led to the establishment of the SEDA, DTI and CIDB. Despite the above governments efforts, SMMEs are still failing, and the factors that contribute to this need to be identified. Not being sustainable restricts economic growth and further widens the gap between the haves and havenots. The research methodology included interviews with contractors, consultants and SMME employees. Data collected were analysed to establish patterns that emerged, and triangulation was employed to consolidate the analysis. The process was conducted against the backdrop of a literature review that explored definitions, international reviews of SMMEs, the experience of SMMEs in developing and developed nations and an evaluation of government support to SMMEs. The research confirmed that SMME employees were generally inexperienced, had a poor understanding of the construction industry and endured huge financial constraints. Appointed Professional Consultants did not include them in project planning, while large appointed Construction companies needed to acknowledge the vital role that they played in the overall state economy. Finally, recommendations are made to develop and sustain SMMEs so that they take their rightful place in the Nelson Mandela Bay Metropole

What is the most effective way of increasing the bioavailability of dietary long chain omega-3 fatty acids-daily vs. weekly administration of fish oil?

The recommendations on the intake of long chain omega-3 polyunsaturated fatty acids (n-3 LC-PUFA) vary from eating oily fish ("once to twice per week") to consuming specified daily amounts of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) ("250-500 mg per day"). It is not known if there is a difference in the uptake/bioavailability between regular daily consumption of supplementsvs. consuming fish once or twice per week. In this study, the bioavailability of a daily dose of n-3 LC-PUFA (Constant treatment), representing supplements, vs. a large weekly dose of n-3 LC-PUFA (Spike treatment), representing consuming once or twice per week, was assessed. Six-week old healthy male Sprague-Dawley rats were fed either a Constant treatment, a Spike treatment or Control treatment (no n-3 LC-PUFA), for six weeks. The whole body, tissues and faeces were analysed for fatty acid content. The results showed that the major metabolic fate of the n-3 LC-PUFA (EPA+docosapentaenoic acid (DPA) + DHA) was towards catabolism (β-oxidation) accounting for over 70% of total dietary intake, whereas deposition accounted less than 25% of total dietary intake. It was found that significantly more n-3 LC-PUFA were β-oxidised when originating from the Constant treatment (84% of dose), compared with the Spike treatment (75% of dose). Conversely, it was found that significantly more n-3 LC-PUFA were deposited when originating from the Spike treatment (23% of dose), than from the Constant treatment (15% of dose). These unexpected findings show that a large dose of n-3 LC-PUFA once per week is more effective in increasing whole body n-3 LC-PUFA content in rats compared with a smaller dose delivered daily

Flight hardware and software operations performance review for BAMMsat-on-BEXUS – a BioCubeSat prototype flown on BEXUS30

BAMMsat-on-BEXUS is a student-led project in which a CubeSat-compatible payload was designed, manufactured, and flown on the BEXUS30 stratospheric balloon. The prototype payload – BAMMsat (Biology, Astrobiology, Medicine, and Materials Science on satellite) – is a modular CubeSat-compatible miniaturised laboratory termed a bioCubeSat. The core flight objective was to perform technology demonstration of the bioCubeSat technology, demonstrating capability to perform experiments in space, and to understand system performance and identify future requirements. The mission aimed to validate pre-flight, flight, and post-flight operations, with a focus on biological and autonomous operations and the novel payload hardware. C. elegans samples were flown in the payload. The mission was partially successful, as the BAMMsat systems and autonomous software operated successfully despite challenging conditions and a large volume of payload performance data was collected; however there were issues maintaining the viability of the samples during flight and microfluidic system issues that impeded sample containment and imaging operations. Post-flight analysis has been performed, the root causes of the issues identified, and upgraded novel payload hardware is currently being developed and tested

BAMMsat-on-BEXUS: A Technology and Operation Demonstration of a BioCubeSat Platform on a Stratospheric Balloon Flight Educational Program

This paper reports the current use of the REXUS/BEXUS educational program. The program allows university students across Europe to carry out scientific and technology experiments on research sounding rockets and balloons. BAMMsat-on-BEXUS (BoB) is an experiment from Cranfield University and University of Exeter performing a technology and operation demonstration of a bioCubeSat on a stratospheric balloon at an altitude of ~30km above the ground. BEXUS stands for Balloon Experiments for University Students and is realized under an agreement between the German Aerospace Centre (DLR), Swedish National Space Agency (SNSA), European Space Agency (ESA), and EuroLaunch. The term bioCubeSat could be used to refer to a nanosatellite in a CubeSat format with a biological experiment on-board. Over the last decade, a series of six bioCubeSats have been launched into orbit by NASA and a private company, SpacePharma, i.e., GeneSat, PharmaSat, O/OREOS, SporeSat, Dido-2, and EcAMSat. The BAMMsat concept (Bioscience, Astrobiology, Medicine and Material science on CubeSat) is a bioscience hardware platform which aims to advance the current state of the art technology, under development at Cranfield University, for application in LEO and beyond LEO. This generic platform can be flown as a free-flying CubeSat or hosted as a payload on a larger spacecraft. BAMMsat utilizes COTS sensors, actuators, and fluidic components to enable bioscience experiments by reproducing the features in a traditional laboratory into a miniaturized “laboratory.” It is designed to be compatible with the mass, volume, and power budget of a CubeSat payload and flexible for a broad range of applications and biological systems such as microorganisms, nematode worms, and mammalian cells cultures, including human cell cultures. The core features of BAMMsat are the ability to (i) house multiple samples, (ii) maintain samples in an appropriate local environment (ii) perturb sample fluidically, and (iv) monitor samples. BoB aims to perform a technology and operation demonstration of the BAMMsat bioCubeSat payload in an extreme environment such as the stratosphere. The experiment is to be flown on the BEXUS30 flight campaign in October 2020 from ESRANGE Space Centre, Sweden. The stratosphere can be used as an analog of some aspect of a relevant spaceflight physical environment such as reduced pressure (near-vacuum; ~11 mbar), and temperature (-50°C). The BEXUS flight campaign could also be used as an analog of pre-flight, flight and post-flight operation similar to orbital launch campaign. For bioscience experiments, the biological samples often imposed additional requirement during pre-flight to ensure its viability. BoB will house C. elegans in a 2U pressure vessel to demonstrate its functionality to provide a controlled thermal and fluidic environment with appropriate housekeeping control. This functionality reflects the hardware capability to maintain a viable biological sample. BoB has a 3U CubeSat form factor with 2U allocated for the BAMMsat hardware and 1U allocated as the BAMMsat-on-BEXUS bus. This paper reports progress at four months before flight campaign. The paper also discusses an overview of the experiment objectives and systems design, to build a representative CubeSat that is translatable into a free-flying orbital CubeSat

A Modular Hardware and Software Architecture for a Student-Designed BioCubeSat Prototype Using Autonomous Operations

BAMMsat-on-BEXUS is a student-led project aiming to design, manufacture, and fly a CubeSat-compatible payload on a stratospheric balloon. The payload – BAMMsat (Biology, Astrobiology, Medicine, and Materials Science on satellite) – is a modular CubeSat-format laboratory termed a bioCubeSat. The mission is realized under the bilateral REXUS/BEXUS programme run by the German Aerospace Center (DLR) and the Swedish National Space Agency (SNSA), with the Swedish payload share available to students through a European Space Agency (ESA) collaboration. The core objective of the prototype payload is to perform a technology demonstration of the core bioCubeSat technology, demonstrating its capability to support biological experiments in space. Additionally, the mission aims to validate pre-flight and flight operations, with a particular focus on biological operations. This will increase TRL for future bioCubeSat spaceflight with the goal to eventually enable better and cheaper biological, pharmaceutical, and materials science research in space environments. The BEXUS mission follows a typical space mission framework with reduced timeframe, therefore trade-offs prioritize commercial-off-the-shelf components and simple software using open-source solutions. The payload comprises a 2U pressurized laboratory payload (BAMMsat) and 1U avionics bus. The former contains experiment hardware including a Multi-Chamber Sample Disc, rotary mechanism, imager, the microfluidics system, active thermal control, and supporting avionics. The bus contains two flight computers, multiple custom avionics PCBs, and serves as the interface between BAMMsat and the BEXUS balloon gondola. The BAMMsat-on-BEXUS prototype will likely fly in October 2021. The prototype flight should prove that the system can perform varied microfluidics operations on multiple C. elegans samples, capture detailed imagery of the samples, provide general system housekeeping and communications, and provide life support for samples, including stable temperature and pressure despite operating within an extreme temperature and near-vacuum environment. The system and biological operations are designed to be fully automatic during flight, with some subsystems continually autonomously operating and others following sequenced events. Future work will aim for greater use of autonomous operations to reduce operating costs and enable more advanced system control, particularly for precise active thermal control and experiment sequencing. The next iteration of BAMMsat is targeting low Earth orbit missions, after further hardware upgrades and the inclusion of fluorescence microscopy and additional chemical sensors

Effectiveness of a multimodal intervention in functionally impaired older people with type 2 diabetes mellitus

Background: Type 2 diabetes, a highly prevalent chronic disease, is associated with increasing frailty and functional decline in older people. We aimed to evaluate the effectiveness of a multimodal intervention on functional performance in frail and pre-frail participants aged >= 70 years with type 2 diabetes mellitus. Methods: The MID-Frail study was a cluster-randomized multicenter clinical trial conducted in 74 trial sites across seven European countries. The trial recruited 964 participants who were aged >70 years [mean age in intervention group, 78.4 (SD 5.6) years, 49.2% male and 77.6 (SD 5.29) years, 52.4% male in usual care group], with type diabetes mellitus and determined to be frail or pre-frail using Fried's frailty phenotype. Participants were allocated by trial site to follow either usual care (UCG) or intervention procedures (IG). Intervention group participants received a multimodal intervention composed of (i) an individualized and progressive resistance exercise programme for 16 weeks; (ii) a structured diabetes and nutritional educational programme over seven sessions; and (iii) Investigator-linked training to ensure optimal diabetes care. Short Physical Performance Battery (SPPB) scores were used to assess change in functional performance at 12 months between the groups. An analysis of the cost-effectiveness of the intervention was undertaken using the incremental cost-effectiveness ratio (ICER). Secondary outcomes included mortality, hospitalization, institutionalization, quality of life, burden on caregivers, the frequency and severity of hypoglycaemia episodes, and the cost-effectiveness of the intervention. Results: After 12 months, IG participants had mean SPPB scores 0.85 points higher than those in the UCG (95% CI, 0.44 to 1.26, P < 0.0001). Dropouts were higher in frail participants and in the intervention group, but significant differences in SPPB between treatment groups remained consistent after sensitivity analysis. Estimates suggest a mean saving following intervention of 428.02 EUR (2016) per patient per year, with ICER analysis indicating a consistent benefit of the described health care intervention over usual care. No statistically significant differences between groups were detected in any of the other secondary outcomes. Conclusions: We have demonstrated that a 12 month structured multimodal intervention programme across several clinical settings in different European countries leads to a clinically relevant and cost-effective improvement in the functional status of older frail and pre-frail participants with type 2 diabetes mellitus

Eicosapentaenoic Acid, Arachidonic Acid and Eicosanoid Metabolism in Juvenile Barramundi Lates calcarifer

A two part experiment was conducted to assess the response of barramundi (Lates calcarifer; initial weight=10.3&plusmn;0.03g; mean&plusmn;S.D.) fed one of five diets with varying eicosapentaenoic acid (diets 1, 5, 10, 15 and 20g/kg) or one of four diets with varying arachidonic acid (1, 6, 12, 18g/kg) against a fish oil control diet. After 6weeks of feeding, the addition of EPA or ARA did not impact on growth performance or feed utilisation. Analysis of the whole body fatty acids showed that these reflected those of the diets. The ARA retention demonstrated an inversely related curvilinear response to either EPA or ARA. The calculated marginal utilisation efficiencies of EPA and ARA were high (62.1 and 91.9% respectively) and a dietary ARA requirement was defined (0.012g/kg0.796/day). The partial cDNA sequences of genes regulating eicosanoid biosynthesis were identified in barramundi tissues, namely cyclooxygenase 1 (Lc COX1a, Lc COX1b), cyclooxygenase 2 (Lc COX2) and lipoxygenase (Lc ALOX-5). BothLc COX2andLc ALOX-5expression in the liver tissue were elevated in response to increasing dietary ARA, meanwhile expression levels ofLcCOX2and the mitochondrial fatty acid oxidation gene carnitine palmitoyltransferase 1 (LcCPT1a) were elevated in the kidney. A low level of EPA increased the expression ofLc COX1bin the liver. Consideration should be given to the EPA to ARA balance for juvenile barramundi in light of nutritionally inducible nature of the cyclooxygenase and lipoxygenase enzymes

Complement Regulates Nutrient Influx and Metabolic Reprogramming during Th1 Cell Responses.

Expansion and acquisition of Th1 cell effector function requires metabolic reprogramming; however, the signals instructing these adaptations remain poorly defined. Here we found that in activated human T cells, autocrine stimulation of the complement receptor CD46, and specifically its intracellular domain CYT-1, was required for induction of the amino acid (AA) transporter LAT1 and enhanced expression of the glucose transporter GLUT1. Furthermore, CD46 activation simultaneously drove expression of LAMTOR5, which mediated assembly of the AA-sensing Ragulator-Rag-mTORC1 complex and increased glycolysis and oxidative phosphorylation (OXPHOS), required for cytokine production. T cells from CD46-deficient patients, characterized by defective Th1 cell induction, failed to upregulate the molecular components of this metabolic program as well as glycolysis and OXPHOS, but IFN-γ production could be reinstated by retrovirus-mediated CD46-CYT-1 expression. These data establish a critical link between the complement system and immunometabolic adaptations driving human CD4(+) T cell effector function

Mission ORCA: orbit refinement for collision avoidance

Forecasting of collisions between resident space objects (RSOs) is becoming critical for the future exploitation of near-Earth space. A constellation of 28 spacecrafts (plus in-orbit spares) in sun synchronous orbits is proposed as a solution for improving the current space situational awareness capabilities. Each satellite uses an optical payload to track target RSOs, with the satellite's position precisely determined. Multiple pictures of the RSO are taken, and the spacecraft attitude used to calculate the target's position relative to the spacecraft. The target's orbit is then determined from the movement of the target through the field of view over time. The system outputs orbit state vectors of the tracked object, allowing precise orbit characterisation and collision forecasting to be delivered. The constellation's design allows high temporal resolution, so reliable information can be supplied to end-users. The paper shows the results of the system design of a demonstration mission meant to verify the feasibility of the concept, performed by a team of students of Cranfield University. The exercise addresses all the aspects of the preliminary design, including the definition of the mission and system requirements, the selection of the overall mission architecture, operations, and mission phases. A cap on the overall cost allows for the realisation of the platform within a university budget. The outline of the design includes not only the selection and sizing of all the subsystems and payload but also suggests a new strategy for deploying the constellation if the demonstration mission is successful. The utilisation of high TRL and COTS components, as well as mass, power, and link budgets, demonstrate the feasibility of the overall mission concept

Mission ORCA: Orbit Refinement for Collision Avoidance

With new launches every year, and the use of 'mega-constellations' becoming commonplace, there is an increasing number of active satellites and other resident space objects (RSOs) in low Earth orbit. However, a collision between objects could be disastrous, having wide-ranging impacts on the collision orbit and all the satellites users within it. Collision forecasting currently has large degrees of uncertainty, causing satellite operators to often ignore collision warnings. It is therefore critical that a system becomes operational to track RSOs and determine the likelihood of collisions with greater accuracy than is currently available. The proposed solution uses a constellation of 28 spacecraft (plus in-orbit spares) in Sun Synchronous Orbits. CubeSats will be used to reduce the cost and the time required for the constellation to become operational. Each satellite uses an optical payload to track target RSOs, with the satellite's position precisely determined. Multiple pictures of the RSO are taken, and the spacecraft attitude used to calculate the target's position relative to the spacecraft. The target's orbit is then determined from the movement of the target through the field of view over time. The system outputs orbit state vectors of the tracked object, allowing precise orbit characterisation and collision forecasting to be delivered. The constellation's design allows high temporal resolution, so reliable information can be supplied to end-users. The paper shows the results of the system design of a demonstration mission meant to verify the feasibility of the concept, performed by a team of students of Cranfield University. The exercise addresses all the aspects of the preliminary design, including the definition of the mission and system requirements, the selection of the overall mission architecture, operations, and mission phases. A cap on the overall cost allows for the realisation of the platform within a university budget. The outline of the design includes not only the selection and sizing of all the subsystems and payload but also suggests a new strategy for deploying the constellation if the demonstration mission is successful. The utilisation of high TRL and COTS components, as well as mass, power, and link budgets, demonstrate the feasibility of the overall mission concep