OS ADVOGADOS CÍVEIS SABEM UTILIZAR A CRASE?

A Língua Portuguesa possibilita inúmeros estudos no campo do Direito. Desta forma, o presente trabalho tem por objetivo revelar, de forma sucinta, se nas petições cíveis o acento indicativo de crase é empregado corretamente. Para investigar como os advogados empregam a crase, aplicou-se a pesquisa documental. Foram analisadas 10 (dez) petições, protocoladas em 2016 e 2017 na 3ª Vara Cível do Tribunal de Justiça do Distrito Federal e Territórios - Circunscrição Judiciária de Ceilândia. Pôdese concluir que uma quantia considerável de ocorrências do acento grave não estava em conformidade com o vernáculo

Desenvolvimento de aplicativo para construção automática de diagramas de reflexões de ondas viajantes

Trabalho de Conclusão de Curso (graduação)—Universidade de Brasília, Faculdade de Tecnologia, Departamento de Engenharia Elétrica, 2017.Nos últimos anos, algoritmos baseados na teoria das ondas viajantes (OVs) vêm ganhando importância em diversas aplicações relacionadas ao diagnóstico de distúrbios em linhas de transmissão (LTs), a exemplo de algoritmos de proteção e localização de faltas, os quais fre- quentemente requerem a análise de diagramas de reexões. Como consequência, metodologias capazes de viabilizar um melhor entendimento dos transitórios decorrentes da propagação de OVs em sistemas de transmissão têm despertado o interesse de concessionárias e pesquisado- res. Nesse sentido, apresenta-se neste trabalho um aplicativo capaz de construir diagramas de reexões de OVs em LTs com circuito simples, duplo ou com linhas adjacentes de forma auto- mática e intuitiva por meio de arquivos de simulação obtidos no software Alternative Transients Program (ATP). Para tanto, a metodologia apresentada faz uso de conceitos e técnicas de l- tragem consolidadas na análise de OVs em LTs, a exemplo da Transformada de Clarke e do ltro Dierentiator-Smoother (ltro DS). Os resultados revelam simplicidade e versatilidade do aplicativo proposto, denominado LAPPICE, o qual demonstra ser uma ferramenta útil durante a análise de transitórios em LTs.In recent decades, algorithms based on traveling waves theory have gained importance in transmission line disturbance diagnosis applications, such as protection and fault location algorithms. These applications often require the analysis of reection diagrams of traveling waves (TWs). Consequently, methodologies that provide a better understanding of transients caused by TWs propagation in transmission systems have aroused the interest of utilities and researchers. Therefore, this work presents an app which is able to automatically build reection diagrams of TWs in transmission lines (TLs) considering single circuit lines, double circuit lines or circuits with adjacents lines in a very intuitive manner through simulation les provided by the software Alternative Transients Program (ATP). The proposed methodology is based on consolidated concepts and ltering techniques widely used in the analysis of TWs, such as the Clarke's Transformation and the Dierentiator-Smoother lter (DS lter). The results reveal simplicity and versatility of the proposed app, called LAPPICE, which has shown to be a useful tool for transients analysis in TLs

Transcriptome analysis and postharvest behavior of the kiwifruit `Actinidia deliciosa¿ reveal the role of ethylene-related phytohormones during fruit ripening

Kiwifruit are climacteric fruit, so they must be harvested before they are fully ripe, allowing for the extension of their shelf-life via cold storage. Therefore, an adequate knowledge about how ethylene-induced fruit senescence is required to avoid significant economic losses. The main goal of the present study was to investigate the kiwifruit ripening process at the physiological and molecular levels by RNA-seq after 1-methylcyclopropene (1-MCP, ethylene inhibitor) and Ethrel® (ethylene stimulator) treatments. The results showed that Ethrel® (ethephon) treatment induced more accelerated fruit ripening, leading to rapid fruit senescence, meanwhile 1-MCP caused a slowing flesh softening, and thus a longer shelf-life period. The RNA-seq was carried out on the fruit after 4 and 13 days, considering day 4 as the most determinant in terms of differentially expressed genes (DEGs). The sequencing achieved 70.7% alignment with the `Hongyang¿ genome, obtaining 18,036 DEGs. The protein-protein interaction (PPI) network shows the interaction between different pathways in two main clusters: (1) pentose and glucoronate interconversions, citrate cycle, glycolysis and gluconeogenesis or starch, and sucrose metabolism and (2) porphyrin and chlorophyll metabolism. The first cluster is mainly interconnected by G6PD1 (pentose pathway); E1 ALPHA and ACLB-2 (citrate cycle); Achn209711 (pentose and glucoronate); LOS2 (glycolysis); HKL1 and HXK1 (glycolysis¿starch and sucrose); and PHS2 (starch and sucrose). In the second cluster, GUN5 through PORA is interacting with CRD1 and NYC1 which were overexpressed by 1-MCP in the porphyrin and chlorophyll metabolism. In addition, genes linked to PSBY and PSBP photosynthesis-linked proteins in photosystem 2 were overexpressed by 1-MCP which is undoubtedly related to chlorophyll degradation and fruit senescence. These results suggest that in kiwifruit, the main pathways that are regulated by ethylene-induced senescence comprise sugar catabolism and chlorophyll degradation.The authors would like to thank the National Commission for Scientific and Technological Research (CONICYT) of Chile government through the project FONDEF N° D09i-1136. Additionally, we thank the Seneca Foundation in the region of Murcia (Spain) through the Regional subprogram “Saavedra Fajardo” (20397/ SF/17) and Ministry of Science and Innovation through the project “Juan de la Cierva Incorporación” N° IJC2018-0366-

Office of the State Auditor FY 2003-2004 Accountability Report

The South Carolina Office of the State Auditor reports to the Office of State Budget its annual accountability report that includes an executive summary, organizational profile including leadership system, program descriptions and budgets, and program results

Childhood pemphigus vulgaris is a challenging diagnosis

Pemphigus Vulgaris (PV) is an uncommon autoimmune and blistering mucocutaneous disease. Childhood Pemphigus Vulgaris (CPV) is a pediatric variant of PV, which affects children below 12 years, being very rare among children under 10 years of age. CPV has similar clinical, histological, and immunological features as seen in PV in adults. The mucocutaneous clinical presentation is the most common in both age groups. Vesicles and erosions arising from the disease usually cause pain. A few CPV cases have been reported in the literature. This study reports a case of an 8-year-old male patient with oral lesions since the age of 3 years, and the diagnosis of pemphigus was achieved only 2 years after the appearance of the initial lesions. CPV remains a rare disease, making the diagnosis of this clinical case a challenge due to its age of onset and clinical features presented by the patient. Therefore, dentists and physicians should know how to differentiate CPV from other bullous autoimmune diseases more common in childhood

Organ-on-a-chip platforms for drug screening and delivery in tumor cells: a systematic review

The development of cancer models that rectify the simplicity of monolayer or static cell cultures physiologic microenvironment and, at the same time, replicate the human system more accurately than animal models has been a challenge in biomedical research. Organ-on-a-chip (OoC) devices are a solution that has been explored over the last decade. The combination of microfluidics and cell culture allows the design of a dynamic microenvironment suitable for the evaluation of treatments’ efficacy and effects, closer to the response observed in patients. This systematic review sums the studies from the last decade, where OoC with cancer cell cultures were used for drug screening assays. The studies were selected from three databases and analyzed following the research guidelines for systematic reviews proposed by PRISMA. In the selected studies, several types of cancer cells were evaluated, and the majority of treatments tested were standard chemotherapeutic drugs. Some studies reported higher drug resistance of the cultures on the OoC devices than on 2D cultures, which indicates the better resemblance to in vivo conditions of the former. Several studies also included the replication of the microvasculature or the combination of different cell cultures. The presence of vasculature can influence positively or negatively the drug efficacy since it contributes to a greater diffusion of the drug and also oxygen and nutrients. Co-cultures with liver cells contributed to the evaluation of the systemic toxicity of some drugs metabolites. Nevertheless, few studies used patient cells for the drug screening assays.This work has been supported by the projects NORTE-01-0145-FEDER-030171 (project reference PTDC/EME-SIS/30171/2017), NORTE-01-0145-FEDER-029394 (project reference PTDC/EMDEMD/29394/2017), through the COMPETE2020, the Lisb@2020, the Programa Operacional Regional do Norte–Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (FEDER) and by Fundação para a Ciência e Tecnologia (FCT), and through FEDER and FCT, project references EXPL/EMD-EMD/0650/2021 and PTDC/EEI-EEE/2846/2021. The authors also acknowledge the partial financial support within the R&D Units Project Scope: UIDB/00319/2020, UIDB/04077/2020, UIDB/00690/2020, UIDB/04436/2020. This work was also funded by AMED-CREST Grant Number JP20gm1310001h0002. Raquel O. Rodrigues (R.O.R.) thanks FCT for her contract funding provided through 2020.03975.CEECIND

A multi-omics analysis of the grapevine pathogen Lasiodiplodia theobromae reveals that temperature affects the expression of virulence- and pathogenicity-related genes

Lasiodiplodia theobromae (Botryosphaeriaceae, Ascomycota) is a plant pathogen and human opportunist whose pathogenicity is modulated by temperature. The molecular effects of temperature on L. theobromae are mostly unknown, so we used a multi-omics approach to understand how temperature affects the molecular mechanisms of pathogenicity. The genome of L. theobromae LA-SOL3 was sequenced (Illumina MiSeq) and annotated. Furthermore, the transcriptome (Illumina TruSeq) and proteome (Orbitrap LC-MS/MS) of LA-SOL3 grown at 25 degrees C and 37 degrees C were analysed. Proteins related to pathogenicity (plant cell wall degradation, toxin synthesis, mitogen-activated kinases pathway and proteins involved in the velvet complex) were more abundant when the fungus grew at 25 degrees C. At 37 degrees C, proteins related to pathogenicity were less abundant than at 25 degrees C, while proteins related to cell wall organisation were more abundant. On the other hand, virulence factors involved in human pathogenesis, such as the SSD1 virulence protein, were expressed only at 37 degrees C. Taken together, our results showed that this species presents a typical phytopathogenic molecular profile that is compatible with a hemibiotrophic lifestyle. We showed that L. theobromae is equipped with the pathogenesis toolbox that enables it to infect not only plants but also animals

3D printing techniques and their applications to organ-on-a-chip platforms: a systematic review

Three-dimensional (3D) in vitro models, such as organ-on-a-chip platforms, are an emerging and effective technology that allows the replication of the function of tissues and organs, bridging the gap amid the conventional models based on planar cell cultures or animals and the complex human system. Hence, they have been increasingly used for biomedical research, such as drug discovery and personalized healthcare. A promising strategy for their fabrication is 3D printing, a layer-by-layer fabrication process that allows the construction of complex 3D structures. In contrast, 3D bioprinting, an evolving biofabrication method, focuses on the accurate deposition of hydrogel bioinks loaded with cells to construct tissue-engineered structures. The purpose of the present work is to conduct a systematic review (SR) of the published literature, according to the guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses, providing a source of information on the evolution of organ-on-a-chip platforms obtained resorting to 3D printing and bioprinting techniques. In the literature search, PubMed, Scopus, and ScienceDirect databases were used, and two authors independently performed the search, study selection, and data extraction. The goal of this SR is to highlight the importance and advantages of using 3D printing techniques in obtaining organ-on-a-chip platforms, and also to identify potential gaps and future perspectives in this research field. Additionally, challenges in integrating sensors in organs-on-chip platforms are briefly investigated and discussed.The authors are grateful for the funding of FCT through the projects NORTE-01-0145- FEDER-029394, NORTE-01-0145-FEDER-030171 funded by COMPETE2020, NORTE2020, PORTUGAL2020, and FEDER. This work was also supported by Fundação para a Ciência e a Tecnologia (FCT) under the strategic grants UIDB/04077/2020, UIDB/00319/2020, UIDB/04436/2020 and UIDB/00532/2020. This work was also funded by AMED-CREST Grant Number JP20gm1310001h0002.Violeta Carvalho acknowledges the PhD scholarship UI/BD/151028/2021 attributed by FCT. Inês Gonçalves acknowledges the PhD scholarship BD/08646/2020 attributed by FCT

Conductividad y evaluación microestructural de Electrolitos sólidos SGDC sintetizados por Pechini y precipitación controlada

In this work, controlled precipitation synthesis methods and polymeric precursors (Pechini) were used to obtain gadolinium-doped cerium oxide solid electrolytes (Ce0.8Gd0.2O1.9) and double-doped cerium oxide with gadolinium and samarium (Ce0.8Gd0.2−xSmxO1.9 x = 0.01; 0.03 and 0.05). The main results indicate that the synthesized powders by the Pechini method present better microstructural characteristics such as larger specific surface area and smaller particle size. Test specimens were compacted (187 MPa) and sintered (1500 °C/6 h) and subsequently characterized. Through the impedance spectroscopy analysis, an increase in the percentage of the samaria caused an increase in the grain boundary and grain conductivity in all the samples under study. In the grain interior, samples without samaria showed heterogeneous regions, where the cation-vacancy pairs are tightly bound, precluding these vacancies from participating in ion conduction. In the samples with samaria, these defect groups are smaller, indicating structural homogeneity, resulting in greater ionic conductivity. Grain boundary specific conductivities show slight increase for samples codoped with samarium oxide. This effect suggests that Sm3+ additions change the charge equilibrium in space-charge layer favoring better dopant distribution.En este trabajo se utilizaron los métodos de síntesis precipitación controlada y precursores poliméricos (Pechini) para obtener electrolitos sólidos de ceria dopada con gadolinio (Ce0.8Gd0.2O1.9) y óxido de ceria doblemente dopada con gadolinio y samario (Ce0.8Gd0.2−xSmxO1.9 x = 0.01; 0.03 and 0.05). Los principales resultados indicaron que los polvos sintetizados por el método Pechini presentaron mejores características microestructurales: mayor área de superficie específica y menor tamaño de partícula. Los polvos fueron compactados (187 MPa), sinterizados (1500 °C/6 h) y, por fin, caracterizados. Con análisis de los espectros de impedancia se observó que el aumento del porcentaje de samario provocó aumento de la conductividad del grano y del contorno de grano en todas las muestras en estudio. En el interior del grano las muestras sin samaria mostraron regiones heterogéneas (grupos de defectos) donde pares catión-vacancia están estrechamente unidos, lo que impide su participación en la conducción iónica. En las muestras con samaria, estos grupos de defectos son más pequeños, indicando homogeneidad estructural, obteniendo una conductividad iónica mas alta. La conductividad específica del límite de grano muestra un ligero aumento para las muestras codopadas con óxido de samario. Este efecto sugiere que las adiciones de Sm3+ cambian el equilibrio de carga en la capa de carga espacial favoreciendo una mejor distribución del dopante