189 research outputs found
The preparation of citrulline by hydrolysis of arginine
The theoretical hydrolysis of arginine to citrulline in vitro has been accomplished and discussed. The details for rapidly and conveniently preparing a 65 gm. quantity of citrulline with the aid of this single reaction have been presented
What Are Antibiotics?
Farmers all over Iowa have read about and heard about antibiotics. And many farmers are using them. What are these substances? Where did they come from? How are they produced? Here\u27s their story up to the present time
Adjuvanted multi-epitope vaccines protect HLA-A*11:01 transgenic mice against Toxoplasma gondii
We created and tested multi-epitope DNA or protein vaccines with TLR4 ligand emulsion adjuvant (gluco glucopyranosyl lipid adjuvant in a stable emulsion [GLA-SE]) for their ability to protect against Toxoplasma gondii in HLA transgenic mice. Our constructs each included 5 of our best down-selected CD8(+) T cell-eliciting epitopes, a universal CD4(+) helper T lymphocyte epitope (PADRE), and a secretory signal, all arranged for optimal MHC-I presentation. Their capacity to elicit immune and protective responses was studied using immunization of HLA-A*11:01 transgenic mice. These multi-epitope vaccines increased memory CD8(+) T cells that produced IFN-γ and protected mice against parasite burden when challenged with T. gondii. Endocytosis of emulsion-trapped protein and cross presentation of the antigens must account for the immunogenicity of our adjuvanted protein. Thus, our work creates an adjuvanted platform assembly of peptides resulting in cross presentation of CD8(+) T cell-eliciting epitopes in a vaccine that prevents toxoplasmosis
The Institutional Sustainability in Protected Area Tourism-Case Studies of Jiuzhaigou National Scenic Area, China and New Forest National Park, United Kingdom
This article considers sustainable tourism development in two protected areas, Jiuzhaigou National Scenic Area in China and the New Forest National Park in the United Kingdom. An inductive approach is used to explore the "fourth component" of sustainable tourism development that is institutional sustainability. Primary data from in-depth interviews, together with a range of secondary data sources, are analyzed to understand the governance and management of each area. These reveal that whilst each area is committed to sustainable development, their approaches differ because of the political, economic, and socio-cultural contexts. The implications for policy and practice are then discussed. © Taylor & Francis Group, LLC
LSST Science Book, Version 2.0
A survey that can cover the sky in optical bands over wide fields to faint
magnitudes with a fast cadence will enable many of the exciting science
opportunities of the next decade. The Large Synoptic Survey Telescope (LSST)
will have an effective aperture of 6.7 meters and an imaging camera with field
of view of 9.6 deg^2, and will be devoted to a ten-year imaging survey over
20,000 deg^2 south of +15 deg. Each pointing will be imaged 2000 times with
fifteen second exposures in six broad bands from 0.35 to 1.1 microns, to a
total point-source depth of r~27.5. The LSST Science Book describes the basic
parameters of the LSST hardware, software, and observing plans. The book
discusses educational and outreach opportunities, then goes on to describe a
broad range of science that LSST will revolutionize: mapping the inner and
outer Solar System, stellar populations in the Milky Way and nearby galaxies,
the structure of the Milky Way disk and halo and other objects in the Local
Volume, transient and variable objects both at low and high redshift, and the
properties of normal and active galaxies at low and high redshift. It then
turns to far-field cosmological topics, exploring properties of supernovae to
z~1, strong and weak lensing, the large-scale distribution of galaxies and
baryon oscillations, and how these different probes may be combined to
constrain cosmological models and the physics of dark energy.Comment: 596 pages. Also available at full resolution at
http://www.lsst.org/lsst/sciboo
Multi-ancestry genome-wide association study accounting for gene-psychosocial factor interactions identifies novel loci for blood pressure traits
Psychological
and social factors are known to influence blood pressure (BP) and risk
of hypertension and associated cardiovascular diseases. To identify
novel BP loci, we carried out genome-wide association meta-analyses of
systolic, diastolic, pulse, and mean arterial BP, taking into account
the interaction effects of genetic variants with three psychosocial
factors: depressive symptoms, anxiety symptoms, and social support.
Analyses were performed using a two-stage design in a sample of up to
128,894 adults from five ancestry groups. In the combined meta-analyses
of stages 1 and 2, we identified 59 loci (p value < 5e−8), including
nine novel BP loci. The novel associations were observed mostly with
pulse pressure, with fewer observed with mean arterial pressure. Five
novel loci were identified in African ancestry, and all but one showed
patterns of interaction with at least one psychosocial factor.
Functional annotation of the novel loci supports a major role for genes
implicated in the immune response (PLCL2), synaptic function and neurotransmission (LIN7A and PFIA2), as well as genes previously implicated in neuropsychiatric or stress-related disorders (FSTL5 and CHODL).
These findings underscore the importance of considering psychological
and social factors in gene discovery for BP, especially in non-European
populations
Miłość jako teologiczne i filozoficzne pojęcie w koncepcji Jana Pawła II
Humankind has been asking the questin about love during the ages. John Paul II took up the theme of Christian love in depth and based it on the theology of body. Pope brought back its real significance by relating this meaning to the betrothal love between woman and man. In his philosophy person is not a means to an end but an end in itself. This love is marked by the common aim, which biases couple in favour of marriage
Multi-ancestry sleep-by-SNP interaction analysis in 126,926 individuals reveals lipid loci stratified by sleep duration.
Both short and long sleep are associated with an adverse lipid profile, likely through different biological pathways. To elucidate the biology of sleep-associated adverse lipid profile, we conduct multi-ancestry genome-wide sleep-SNP interaction analyses on three lipid traits (HDL-c, LDL-c and triglycerides). In the total study sample (discovery + replication) of 126,926 individuals from 5 different ancestry groups, when considering either long or short total sleep time interactions in joint analyses, we identify 49 previously unreported lipid loci, and 10 additional previously unreported lipid loci in a restricted sample of European-ancestry cohorts. In addition, we identify new gene-sleep interactions for known lipid loci such as LPL and PCSK9. The previously unreported lipid loci have a modest explained variance in lipid levels: most notable, gene-short-sleep interactions explain 4.25% of the variance in triglyceride level. Collectively, these findings contribute to our understanding of the biological mechanisms involved in sleep-associated adverse lipid profiles
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