17 research outputs found
Compositional Assessments of Key Maize Populations: B73 Hybrids of the Nested Association Mapping Founder Lines and Diverse Landrace Inbred Lines
The
present study provides an assessment of the compositional diversity
in maize B73 hybrids derived both from the Nested Association Mapping
(NAM) founder lines and from a diverse collection of landrace accessions
from North and South America. The NAM founders represent a key population
of publicly available lines that are used extensively in the maize
community to investigate the genetic basis of complex traits. Landraces
are also of interest to the maize community as they offer the potential
to discover new alleles that could be incorporated into modern maize
lines. The compositional analysis of B73 hybrids from the 25 NAM founders
and 24 inbred lines derived from landraces included measurements of
proximates (protein, fat, ash, and starch), fibers, minerals, amino
acids, fatty acids, tocopherols (α-, γ-, and δ-),
β-carotene, phytic acid, and raffinose. Grain was harvested
from a replicated trial in New York, USA. For each data set (NAM and
landrace) canonical discriminant analysis allowed separation of distinct
breeding groups (tropical, temperate, flint, mixed/intermediate) within
each data set. Overall, results highlighted extensive variation in
all composition components assessed for both sets of hybrids. The
variation observed for some components within the landraces may therefore
be of value for increasing their levels in modern maize lines. The
study described here provided significant information on contributions
of conventional breeding to crop compositional variation, as well
as valuable information on key genetic resources for the maize community
in the development of new improved lines
Compositional Assessments of Key Maize Populations: B73 Hybrids of the Nested Association Mapping Founder Lines and Diverse Landrace Inbred Lines
The
present study provides an assessment of the compositional diversity
in maize B73 hybrids derived both from the Nested Association Mapping
(NAM) founder lines and from a diverse collection of landrace accessions
from North and South America. The NAM founders represent a key population
of publicly available lines that are used extensively in the maize
community to investigate the genetic basis of complex traits. Landraces
are also of interest to the maize community as they offer the potential
to discover new alleles that could be incorporated into modern maize
lines. The compositional analysis of B73 hybrids from the 25 NAM founders
and 24 inbred lines derived from landraces included measurements of
proximates (protein, fat, ash, and starch), fibers, minerals, amino
acids, fatty acids, tocopherols (α-, γ-, and δ-),
β-carotene, phytic acid, and raffinose. Grain was harvested
from a replicated trial in New York, USA. For each data set (NAM and
landrace) canonical discriminant analysis allowed separation of distinct
breeding groups (tropical, temperate, flint, mixed/intermediate) within
each data set. Overall, results highlighted extensive variation in
all composition components assessed for both sets of hybrids. The
variation observed for some components within the landraces may therefore
be of value for increasing their levels in modern maize lines. The
study described here provided significant information on contributions
of conventional breeding to crop compositional variation, as well
as valuable information on key genetic resources for the maize community
in the development of new improved lines
Compositional Assessments of Key Maize Populations: B73 Hybrids of the Nested Association Mapping Founder Lines and Diverse Landrace Inbred Lines
The
present study provides an assessment of the compositional diversity
in maize B73 hybrids derived both from the Nested Association Mapping
(NAM) founder lines and from a diverse collection of landrace accessions
from North and South America. The NAM founders represent a key population
of publicly available lines that are used extensively in the maize
community to investigate the genetic basis of complex traits. Landraces
are also of interest to the maize community as they offer the potential
to discover new alleles that could be incorporated into modern maize
lines. The compositional analysis of B73 hybrids from the 25 NAM founders
and 24 inbred lines derived from landraces included measurements of
proximates (protein, fat, ash, and starch), fibers, minerals, amino
acids, fatty acids, tocopherols (α-, γ-, and δ-),
β-carotene, phytic acid, and raffinose. Grain was harvested
from a replicated trial in New York, USA. For each data set (NAM and
landrace) canonical discriminant analysis allowed separation of distinct
breeding groups (tropical, temperate, flint, mixed/intermediate) within
each data set. Overall, results highlighted extensive variation in
all composition components assessed for both sets of hybrids. The
variation observed for some components within the landraces may therefore
be of value for increasing their levels in modern maize lines. The
study described here provided significant information on contributions
of conventional breeding to crop compositional variation, as well
as valuable information on key genetic resources for the maize community
in the development of new improved lines
Metabolomic Assessment of Key Maize Resources: GC-MS and NMR Profiling of Grain from B73 Hybrids of the Nested Association Mapping (NAM) Founders and of Geographically Diverse Landraces
The
present study expands metabolomic assessments of maize beyond
commercial lines to include two sets of hybrids used extensively in
the scientific community. One set included hybrids derived from the
nested association mapping (NAM) founder lines, a collection of 25
inbreds selected on the basis of genetic diversity and used to investigate
the genetic basis of complex plant traits. A second set included 24
hybrids derived from a collection of landraces representative of native
diversity from North and South America that may serve as a source
of new alleles for improving modern maize hybrids. Metabolomic analysis
of grain harvested from these hybrids utilized gas chromatography–time-of-flight
mass spectrometry (GC-TOF-MS) and <sup>1</sup>H nuclear magnetic resonance
spectroscopy (<sup>1</sup>H NMR) techniques. Results highlighted extensive
metabolomic variation in grain from both hybrid sets, but also demonstrated
that, within each hybrid set, subpopulations could be differentiated
in a pattern consistent with the known genetic and compositional variation
of these lines. Correlation analysis did not indicate a strong association
of the metabolomic data with grain nutrient composition, although
some metabolites did show moderately strong correlations with agronomic
features such as plant and ear height. Overall, this study provides
insights into the extensive metabolomic diversity associated with
conventional maize germplasm
Metabolomic Assessment of Key Maize Resources: GC-MS and NMR Profiling of Grain from B73 Hybrids of the Nested Association Mapping (NAM) Founders and of Geographically Diverse Landraces
The
present study expands metabolomic assessments of maize beyond
commercial lines to include two sets of hybrids used extensively in
the scientific community. One set included hybrids derived from the
nested association mapping (NAM) founder lines, a collection of 25
inbreds selected on the basis of genetic diversity and used to investigate
the genetic basis of complex plant traits. A second set included 24
hybrids derived from a collection of landraces representative of native
diversity from North and South America that may serve as a source
of new alleles for improving modern maize hybrids. Metabolomic analysis
of grain harvested from these hybrids utilized gas chromatography–time-of-flight
mass spectrometry (GC-TOF-MS) and <sup>1</sup>H nuclear magnetic resonance
spectroscopy (<sup>1</sup>H NMR) techniques. Results highlighted extensive
metabolomic variation in grain from both hybrid sets, but also demonstrated
that, within each hybrid set, subpopulations could be differentiated
in a pattern consistent with the known genetic and compositional variation
of these lines. Correlation analysis did not indicate a strong association
of the metabolomic data with grain nutrient composition, although
some metabolites did show moderately strong correlations with agronomic
features such as plant and ear height. Overall, this study provides
insights into the extensive metabolomic diversity associated with
conventional maize germplasm