Effect of a Fibrolytic Enzymatic Extract from Cellulomonas flavigena on In Vitro Degradation and In Vivo Digestibility and Productive Performance of Lambs

An enzymatic extract from Cellulomonas flavigena was evaluated at 0, 2.5, 7.5, 12.5 mL/kg DM of total mixed ration (TMR) on the in vitro degradation of DM, NDF and ADF and in vivo at 0, 5.0 and 7.5 mL of extract per kg DM of TMR to determine the digestibility and productive performance of lambs fed a TMR made up of 60% forage. Twenty four Pelibuey-Kathadin lambs were used in the trial. The in vitro degradation of ADF showed a linear (P<0.05) response from 6 to 72 h. There was no effect on DM intake, daily gain or feed conversion. The enzymatic dose tended to linearly decrease the apparent digestibility of DM (P=0.06), NDF (P=0.10) and ADF (P=0.06). The N-NH3 concentration showed a linear decrease (P=0.002) and total VFA concentration was linearly (P<0.001) increased. The incorporation of extract of Cellulomonas flavigena in the diet increased in vitro degradation of cellulose in terms of ADF but did not increase the digestion or productive performance of lambs

Manifold analysis of the P-wave changes induced by pulmonary vein isolation during cryoballoon procedure

Background/Aim: In atrial fibrillation (AF) ablation procedures, it is desirable to know whether a proper disconnection of the pulmonary veins (PVs) was achieved. We hypothesize that information about their isolation could be provided by analyzing changes in P-wave after ablation. Thus, we present a method to detect PV disconnection using P-wave signal analysis. Methods: Conventional P-wave feature extraction was compared to an automatic feature extraction procedure based on creating low-dimensional latent spaces for cardiac signals with the Uniform Manifold Approximation and Projection (UMAP) method. A database of patients (19 controls and 16 AF individuals who underwent a PV ablation procedure) was collected. Standard 12-lead ECG was recorded, and P-waves were segmented and averaged to extract conventional features (duration, amplitude, and area) and their manifold representations provided by UMAP on a 3-dimensional latent space. A virtual patient was used to validate these results further and study the spatial distribution of the extracted characteristics over the whole torso surface. Results: Both methods showed differences between P-wave before and after ablation. Conventional methods were more prone to noise, P-wave delineation errors, and inter-patient variability. P-wave differences were observed in the standard leads recordings. However, higher differences appeared in the torso region over the precordial leads. Recordings near the left scapula also yielded noticeable differences. Conclusions: P-wave analysis based on UMAP parameters detects PV disconnection after ablation in AF patients and is more robust than heuristic parameterization. Moreover, additional leads different from the standard 12-lead ECG should be used to detect PV isolation and possible future reconnections better

Rol del aislamiento histórico y del cambio en el nicho climático en la diversificación de las plantas con flores en los Andes australes: El caso de Gomphrena (Amaranthaceae)

El levantamiento de la cordillera de los Andes es uno de los eventos que tuvo mayor impacto en la historia biogeográfica de la biota sudamericana, debido no sólo a su interposición física, sino también a su compleja topografía, la cual contribuyó al desarrollo de condiciones abióticas muy diversas a lo largo del continente. En el presente trabajo se analiza la influencia del levantamiento de los Andes australes en la diversificación de las especies del género Gomphrena (Amaranthaceae) que habitan el Cono Sur, evaluando el rol del cordón montañoso como generador de eventos de vicarianza, promoviendo el intercambio de elementos de la biota como un corredor, o bien promoviendo nuevas condiciones ambientales tanto dentro como fuera de la propia cordillera. Estudios previos han encontrado que las especies altoandinas de Gomphrena conforman un clado, mientras que las especies ampliamente distribuídas o de regiones bajas se agrupan en otro. A partir de las relaciones filogenéticas conocidas y de los datos de distribución disponibles para el género, se estimaron los tiempos de divergencia, las distribuciones ancestrales y la evolución del nicho climático a lo largo de los Andes australes. Finalmente, se compararon estos resultados con los observados en sistemas co-distribuidos con Gomphrena para determinar los principales procesos en los que el cordón montañoso podría haber actuado como generador de biodiversidad. Con ello, se espera obtener un panorama general en el tiempo y el espacio sobre el rol de la cordillera de los Andes en la diversificación de la flora sudamericana.Fil: Bena, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; ArgentinaFil: Baranzelli, Matias Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Cosacov Martinez, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Acosta, María Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Moreira Muñoz, Andrés Sergio. Pontificia Universidad Católica de Valparaíso; ChileLXIV Reunión Anual Sociedad de Biología de Chile; XXVII Reunión Anual Sociedad de Ecología de Chile y XXXII Reunión Anual Sociedad de Botánica de ChileSantiagoChileSociedad de Biología de ChileSociedad de Botánica de ChileSociedad de Ecología de Chil

An Eulerian-Lagrangian open source solver for bubbly flow in vertical pipes

Air-water two-phase flow is present in natural and industrial processes of different nature as nuclear reactors. An accurate local prediction of the boiling flow could support safety and operation analyses of nuclear reactors. A new Eulerian-Lagrangian approach is investigated in this contribution. A new solver has been developed and implemented in the framework of the open source package OpenFOAM R and based on the PIMPLE algorithm coupled with the Lagrangian equation of motion has been implemented for computing incompressible bubbly flows. Each bubble is divided in equivolumetric volumes and tracked into the Eulerian mesh for an appropriate assignment of the effect of the bubble in the cell without resolving the interface. The coupling between phases is done considering in the momentum equation the interfacial forces and bubble induced contribution along the bubble path during an Eulerian time step. The bouncing of the bubbles between themselves and the wall is modeled with a dynamic soft sphere model. The computational results obtained for different flow conditions are validated with the recently released experimental data on upward pipe flow. The test section used is a 52 mm pipe of 5500 mm of length maintained under adiabatic conditions with air and water circulating fluids working with inlet velocity ranges of 0-2 m/s and 0-0.3 m/s for the continuous and dispersed phase respectively. Averaged results of radial distribution for void fraction, chord length, turbulence kinetic energy, dispersed and continuous velocity profiles show a good agreement among different flow conditions.Peña Monferrer, C.; Muñoz-Cobo González, JL.; Monrós Andreu, G.; Martinez Cuenca, R.; Chiva Vicent, S. (2014). An Eulerian-Lagrangian open source solver for bubbly flow in vertical pipes. Sociedad Nuclear Española. http://hdl.handle.net/10251/71943

Cross-cultural adaptation and psychometric evaluation of the early childhood oral health impact scale (ECOHIS) in chilean population

The Early Childhood Oral Health Impact Scale (ECOHIS) measures the impact of dental diseases on Oral Health-Related Quality of Life both in children and their families. The aim of this study was to develop a Chilean Spanish version of the ECOHIS that is conceptually equivalent to the original and to assess its acceptability, reliability and validity in the preschool population of Chile. The Chilean version of the ECOHIS was obtained through a process including forward and back-translation, expert panel, and cognitive debriefing interviews. To assess metric properties, a cross-sectional study was carried out in Carahue, Southern Chile (April-October 2016). Children younger than six years old without systemic diseases, disabilities or chronic medication from eleven public preschools were included. Parents were invited to complete the Chilean version of the ECOHIS, PedsQL™4.0 Generic Core and PedsQL Oral Health scales, and to answer global questions about their children's general and oral health. A subsample was administrated ECOHIS a second time 14-21 days after. A clinical examination was performed to assess dental caries, malocclusion, and traumatic dental injuries. Reliability was evaluated using measures of internal consistency (Cronbach's alpha) and reproducibility (Intraclass correlation coefficient - ICC). Construct validity was assessed by testing hypotheses based on available evidence about known groups and relationships between different instruments. The content comparison of the back-translation with the original ECOHIS showed that all items except one were conceptually and linguistically equivalent. The cognitive debriefing showed a suitable understanding of the Chilean version by the parents. In the total sample (n = 302), the ECOHIS total score median was 1 (IQR 6), floor effect was 41.6%, and ceiling effect 0%. Cronbach's alpha was 0.89 and the ICC was 0.84. The correlation between ECOHIS and PedsQL™4.0 Generic Core was weak (r = 0.21), while it was strong-moderate (r = 0.64) with the PedsQL Oral Health scale. In the known groups comparison, the ECOHIS total score was statistically higher in children with poor than excellent/very good oral health (median 11.6 vs 0, p < 0.01), and in the high severity than in the caries-free group (median 8 vs 0.5, p < 0.01). No differences were found according to malocclusion and traumatic dental injuries groups. These results supported the feasibility, reliability and validity of the Chilean version of ECOHIS questionnaire for preschool children through proxy

Más allá de la apariencia: imagen corporal asociada a las actitudes y conductas de riesgo de trastorno alimentario en adolescentes

Artículo de investigación publicado en la Revista de Psicología de la Universidad del Estado de MéxicoAnte los cambios biopsicosociales de la adolescencia algunas personas presentan alteraciones psicológicas asociadas al peso, alimentación e imagen corporal. Por tal motivo, el presente estudio tuvo como objetivo, identificar la insatisfacción corporal y el nivel de riesgo de trastornos alimentarios, así como, analizar la relación de la imagen corporal con las actitudes y conductas alimentarias de riesgo en adolescentes de ambos sexos, habitantes del Estado de México. Participaron 131 adolescentes de ambos sexos, que contestaron tres instrumentos: Cuestionario de imagen corporal, Cuestionario de actitudes alimentarias y Cuestionario de conductas alimentarias de riesgo. Los resultados indican no existen diferencias significativas en las variables evaluadas entre mujeres y hombres. Se encontró un 8.4% de insatisfacción corporal y 6.1% actitudes alimentarias de riesgo, no existieron casos de riesgo alto en las conductas alimentarias de riesgo, ni conductas compensatoria; la insatisfacción corporal se relaciona con la preocupación por peso y figura y la dieta restrictiva. Se concluye, que la sobrevaloración de la imagen corporal se asocia con actitudes y conductas que pueden perjudicar el desarrollo saludable del adolescente. Se requieren intervenciones de promoción desarrollo saludable del adolescente y prevención de trastornos alimentarios en ambientes educativos y comunitarios

Relationship between psychopathological symptoms and quality of life in women and men

Los problemas de salud mental afectan el desarrollo biopsicosocial de las personas. Dichos problemas empeoran debido a que se acude tardíamente a los servicios de salud, evidenciando la necesidad de detecciones tempranas en población abierta. Por ello, el objetivo de esta investigación fue evaluar la relación entre síntomas psicopatológicos y calidad de vida en mujeres y hombres. Participaron 465 personas, habitantes del Estado de México, quienes contestaron el SCL-90-R y el WHOQOL-BREF. En los resultados, las mujeres presentan mayores puntuaciones de síntomas psicopatológicos y menor calidad de vida con respecto a los hombres. Los problemas de salud mental afectan la calidad de vida por diferentes vías de influencia de acuerdo al sexo; en las mujeres destaca la depresión y la sintomatología global; y en los hombres, la somatización, la sintomatología global y la depresión. Se discute sobre la necesidad de prevención y detección precoz de los síntomas incipientes a través del trabajo comunitario.El presente estudio ha sido financiado por la Universidad Autónoma del Estado de México y por la Secretaria de Educación Pública

Effects of different plyometric training frequencies on physical performance in youth male volleyball players: a randomized trial

This study aimed to analyze the effect of plyometric training (PT) at different frequencies on jump performance, running sprint speed, and service speed in youth male volleyball players. The participants were randomly assigned to one PT session per week (Experimental Group 1, EG1, n = 15), two PT sessions per week (Experimental Group 2, EG2, n = 14), and a control group (CG, n = 13). The total weekly jumping ranged between 98 and 196 jumps (equalized between, EG1 and, EG2). The assessments performed were squat jump (SJ), countermovement jump (CMJ), CMJ-arms, drop jump (DJ), 5-m sprint, 10-m sprint, and service speed. The intragroup comparisons showed that, EG1 significantly (p &lt; 0.001) improved SJ (Δ = 12.74%; d = 1.30), CMJ (Δ = 11.94%; d = 1.71), CMJ-arms (Δ = 12.02%; d = 1.47), DJ (Δ = 10.93%; d = 1.30), 5-m sprint (Δ = −4.61%; d = 0.29), 10-m sprint (Δ = −3.95%; d = 0.40) and service speed (Δ = 8.17%; d = 1.53). Similarly, EG2 significantly (p˂ 0.001) improved SJ (Δ = 11.52%; d = 1.25), CMJ (Δ = 11.29%; d = 1.38), CMJ-arms (Δ = 11.42%; d = 1.26), DJ (Δ = 13.90%; d = 2.17), 5-m sprint (Δ = −3.85%; d = 0.25), 10-m sprint (Δ = −2.73%; d = 0.25) and service speed (Δ = 6.77%; d = 1.44). The CG significantly (p &lt; 0.05) improved SJ (Δ = 2.68; d = 0.28), CMJ-arms (Δ = 2.30; d = 0.35), 5-m sprint (Δ = −1.27; d = 0.10) and service speed (Δ = 1.42; d = 0.30). Intergroup comparisons revealed significantly greater improvements in all variables (p &lt; 0.001) in, EG1 and, EG2 concerning to CG. However, no significant differences were found between, EG1 and, EG2. A moderate weekly PT volume, distributed in one or two sessions per week, seems equally effective

Afrontamiento y síntomas de estrés postraumático en niños mexicanos después del sismo de 2017.

Se analiza la relación entre el afrontamiento frente a riesgos extremos y los síntomas de estrés postraumático en niños que experimentaron el terremoto de México de 2017. Al mes del evento, se evaluaron 178 niños(as) entre 11 y 15 años de edad (M = 12.90, DE = 1.05) de Malinalco, municipio al sur del Estado de México, con la escala de afrontamiento frente a riesgos extremos y la escala infantil de síntomas del trastorno de estrés postraumático (TEPT). Los resultados mostraron puntajes altos en TEPT en las niñas. Asimismo, el afrontamiento tanto activo como pasivo aumenta débilmente la presencia de síntomas de TEPT. Se sugiere analizar la capacidad resiliente ante este tipo de desastres naturales, así como darle un seguimiento.Esta investigación forma parte del Proyecto de Investigación titulado “Mediadores y Moderadores de la Resiliencia en niños y adolescentes”. y registrado en la UAEM con clave 4222/2016SF, así como del proyecto con clave 4645/2019SF y con registro del Comité de Etica de la UAEM (Clave: 2019/05)

The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity

The RNA polymerase II-associated protein 1 (RPAP1) is conserved across metazoa and required for stem cell differentiation in plants; however, very little is known about its mechanism of action or its role in mammalian cells. Here, we report that RPAP1 is essential for the expression of cell identity genes and for cell viability. Depletion of RPAP1 triggers cell de-differentiation, facilitates reprogramming toward pluripotency, and impairs differentiation. Mechanistically, we show that RPAP1 is essential for the interaction between RNA polymerase II (RNA Pol II) and Mediator, as well as for the recruitment of important regulators, such as the Mediator-specific RNA Pol II factor Gdown1 and the C-terminal domain (CTD) phosphatase RPAP2. In agreement, depletion of RPAP1 diminishes the loading of total and Ser5-phosphorylated RNA Pol II on many genes, with super-enhancer-driven genes among the most significantly downregulated. We conclude that Mediator/RPAP1/RNA Pol II is an ancient module, conserved from plants to mammals, critical for establishing and maintaining cell identity.We are grateful to Elisa Varela for assistance with morula and blastocyst fixa- tion. Work in the laboratory of M.S. is funded by the CNIO and the IRB and by grants from the Spanish Ministry of Economy co-funded by the European Regional Development Fund (ERDF) (SAF2013-48256-R), the European Research Co uncil (ERC-2014-AdG/66 9622), the Region al Government of Ma- drid co-funded by the Euro pean Social Fund (ReCaRe project), the Euro pean Union (RISK-IR project), the Botin Foundation and Banco Santander (Santander Universities Glo bal Division), the Ramon Areces Found ation, and the AXA Foundation. S.R. was funded by a contract from the Ramon y Cajal Program(RYC-2011-09242) and by the Spanish Ministry of Economy co- funded by the ERDF (SAF2013-49147- P and SAF2016-80874-PS