Genetic structure of Tribolium castaneum populations in mills

We investigated the genetic diversity and differentiation among nine populations of Tribolium castaneum using eight polymorphic loci, including microsatellites and other insertion-deletion polymorphisms (=”indels”). Samples were collected in food processing/storage facilities located in Kansas, Nebraska, California, Louisiana, Florida and Puerto Rico. Standard population genetic analysis was applied, and an assignment test was used to assign individuals to their genetic population. All loci were polymorphic across populations, with the number of alleles per locus-population combination varying from three to fourteen. Among 72 locus-by-population combinations, 31 deviated significantly from Hardy-Weinberg equilibrium, which was associated with a deficiency in heterozygosity. Tribolium castaneum populations show some level of genetic structuring. Genetic differentiation between populations, using FST estimates, was significant, with FST varying from 0.018 to 0.149. AMOVA indicated that 8.32% of the variation in allele frequency resulted from comparisons among populations. Genetic distance was not significantly correlated with geographic distance. Correct assignment to the genetic population was possible in only 56% of all individuals. Together, these results revealed that geographically distinct populations of T. castaneum had low to moderate levels of genetic differentiation that was not correlated with geographic distance, and the genotypic profile of the individuals did not provide enough information for fingerprinting them with their source population. Keywords: Tribolium castaneum, Population genetics, Genetic structure, FST, Genetic fingerprintin

Impaired Bone Health in Inflammatory Bowel Disease: A Case-Control Study in 80 Pediatric Patients

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Response of \u3ci\u3eTribolium castaneum and Tribolium confusum \u3c/i\u3e adults to vertical black shapes and its potential to improve trap capture

Tribolium castaneum and Tribolium confusum can be monitored in food processing facilities using traps baited with pheromones and kairomones, but beetle response to traps might be enhanced by adding visual cues. Against a white background, T. castaneum adults were more likely to visit black pillars than white pillars when presented with a choice (e.g., 73% of beetles visited black and 17% visiting white pillar), and visits to black pillars increased with pillar height. When tested against a black background, beetles did not show a significant preference for either color pillar regardless of height. When comparing beetle’s captures in pheromone/kairomone baited traps placed in front of a white or black panel in a white arena under high, low, or dark light conditions, more beetles were captured in traps in front of black panels under both high and low light conditions, but not under dark conditions. A similar pattern of capture under low light and dark conditions was also found for the closely related species T. confusum. In a larger scale choice test, the same pattern of greater T. castaneum captures in traps in front of black panels than white panels was obtained, whether traps were placed in corners or along walls. Our results suggest that captures in monitoring traps could be increased by adding dark vertical shapes behind trap locations or placing traps near dark structures