Estagio supervisionado realizado na empresa marine maricultura do Nordeste

Trabalho de Conclusão de Curso - Universidade Federal de Santa Catarina. Curso de Agronomia

Modelo de planejamento para a gestão territorial da carcinicultura marinha

Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia Civil

Eco-efficiency assessment of shrimp aquaculture production in Mexico

Globally, human society faces the challenge of providing food to a growing population, at the same time that the effects of climate change and resource depletion must be addressed. Aquaculture allows to ensure a safe supply of different marine species and is a major technological and biological undertaking. Taking into account that in Sonora (Mexico), there are more than 200 aquaculture plants, the analysis of this sector implies a joint and harmonized assessment, considering not only life cycle assessment (LCA), but also data envelopment analysis (DEA). This study focuses on the application of LCA + DEA methodology to assess the ecoefficiency of 38 semi-intensive shrimp farms located in the state of Sonora. LCA results showed that feed management and electricity consumption are the main critical points in almost all the impact categories. Further improvement actions were evaluated, the replacement of wheat meal for Dried Distiller Grains with Solubles (DDGS) resulted in environmental impact reductions ranged from 2% to 57%, depending on the impact category. On the other hand, the installation of photovoltaic panels in the area was evaluated, looking for a shift towards a less carbon-intensive energy production. Overall, the implementation of these improvement measures will contribute to increased environmental protection and resource efficiencyThis research was supported by the Comité de Sanidad Acuícola del Estado de Sonora (COSAES). A. Cortés, C. Cambeses Franco, G. Feijoo and M.T. Moreira belong to CRETUS and the Galician Competitive Research Group GRC (ED431C 2017/29), co-founded by Xunta de Galicia and FEDER (EU)S

Prevendo os níveis de poluição fecal na água e nos moluscos produzidos em fazendas marinhas das baías da Ilha de Santa Catarina

Mathematical models have been used around the world to predict pollution levels in coastal areas and to communicate to the public when safety for bathing or shellfish consumption may be compromised. Recent studies conducted by Epagri have developed regression-based models that allow predicting the coliform levels in areas used for mollusc farming in the North and South bays of Santa Catarina Island. The present document presents the main findings of these studies in a didactic way and suggests practical applications for technicians in the area of mariculture, fishing and water resources management in the region.Modelos matemáticos vêm sendo utilizados ao redor do mundo para prever os níveis de poluição em áreas costeiras e comunicar ao público quando a segurança para banho ou consumo de moluscos comprometido. Estudos recentes da Epagri desenvolveram modelos baseados em regressão que permitem prever os níveis de coliformes em áreas utilizadas para o cultivo de moluscos nas baías Norte e Sul da Ilha de Santa Catarina. Este trabalho apresenta os principais achados desses estudos de forma didática e sugere aplicações práticas para técnicos da área de maricultura, pesca e gestão de recursos hídricos que atuam na região.

Assessment of algorithms for mitosis detection in breast cancer histopathology images

The proliferative activity of breast tumors, which is routinely estimated by counting of mitotic figures in hematoxylin and eosin stained histology sections, is considered to be one of the most important prognostic markers. However, mitosis counting is laborious, subjective and may suffer from low inter-observer agreement. With the wider acceptance of whole slide images in pathology labs, automatic image analysis has been proposed as a potential solution for these issues. In this paper, the results from the Assessment of Mitosis Detection Algorithms 2013 (AMIDA13) challenge are described. The challenge was based on a data set consisting of 12 training and 11 testing subjects, with more than one thousand annotated mitotic figures by multiple observers. Short descriptions and results from the evaluation of eleven methods are presented. The top performing method has an error rate that is comparable to the inter-observer agreement among pathologists

Substitution of fish meal in diets for marine shrimp grown in a biofloc system

O objetivo deste trabalho foi avaliar a substituição de dois níveis de inclusão de farinha de peixe por outros ingredientes, nas dietas de Litopenaeus vannamei cultivados em sistema bioflocos. Foram avaliadas dietas sem uso de farinha de peixe (dieta A, 100% de substituição), com inclusão de 12,5% de farinha de peixe (dieta B, 40% de substituição) e direta controle com inclusão de 21% (dieta C, 0% de substituição). Nas dietas A e B, o farelo de soja e as farinhas de carne e vísceras foram os principais substitutos proteicos. Foram analisados os índices de desempenho dos camarões e os parâmetros físicos e químicos da água de cultivo. Os camarões alimentados com a dieta B apresentaram maior peso final (11,63±1,38 g), em comparação aos camarões alimentados com a dieta A (peso final, 9,39±0,31 g) e com a dieta C (peso final, 10,20±1,10 g). Os demais parâmetros de desempenho como produtividade, conversão alimentar e sobrevivência não apresentaram diferenças entre os tratamentos. A redução de até 40,0% da farinha de peixe pode ser feita em cultivos superintensivos de L. vannamei com bioflocos, sem interferir em seu desempenho zootécnico e na qualidade de água do cultivo. The objective of this work was to evaluate the substitution of two fish meal inclusion levels by other ingredients, in the diets of Litopenaeus vannamei reared in a biofloc system. The following diets were evaluated: without fish meal (diet A, 100% replacent), with addition of 12.5% of fish meal (diet B, 40% replacent), and a control diet with addition of 21% (diet C, 0% replacent). In diets A and B, soybean meal and meat and viscera meals were the main protein replacement. The performance indexes of shrimps and the physical and chemical parameters of water were analyzed. Shrimp fed diet B had higher final weight (11.63±1.38 g), compared to shrimp fed a diet A (9.39±0.31 g final weight) or C (10.20±1.10 g final weight). The other parameters of performance such as yield, feed conversion and survival did not differ between treatments. The reduction of fish meal by 40.0% can be done in a L. vannamei superintensive biofloc system without interfering on their growth performance or on the water quality of the culture

Methods for determining the apparent digestibility of diets for marine shrimp supplemented with probiotic

O objetivo deste trabalho foi determinar o método de coleta de fezes mais adequado para camarões marinhos (Litopenaeus vannamei) na determinação do coeficiente de digestibilidade aparente da proteína bruta e da matéria seca de dietas comerciais, e avaliar o efeito da suplementação de Lactobacillus plantarum na dieta sobre o coeficiente de digestibilidade aparente da proteína, da energia e da matéria seca. Foram testados três sistemas de coletas de fezes: decantação, sifonamento e dissecação dos animais. A coleta por decantação foi o sistema mais adequado para a determinação in vivo dos coeficientes de digestibilidade aparente da proteína bruta e da matéria seca das dietas, por apresentar baixo grau de lixiviação das fezes e por ser de fácil execução. Nos camarões alimentados com a dieta suplementada com L. plantarum, foi observado maior coeficiente de digestibilidade aparente da proteína (84,18±0,35%) e da energia (81,28±0,16%), quando comparado ao grupo controle (82,73±1,01 e 78,63±0,93%, respectivamente). O uso de L. plantarum como aditivo probiótico na ração pode trazer benefícios nutricionais a L. vannamei, com melhor aproveitamento dos nutrientes.The objective of this work was to determine the most adequate feces collection method for sea shrimp (Litopenaeus vannamei) in the determination of the apparent digestibility coefficients of crude protein and dry matter of commercial diets, and to evaluate the effect of supplementation of Lactobacillus plantarum to the diet on the apparent digestibility coefficient of protein, energy, and dry matter. Three feces collection methods were tested: decanting, siphoning, and animal dissection. Decanting was the method best suited for the in vivo determination of the apparent digestibility coeffcients of crude protein and dry matter of commercial diets, since it promoted lower nutrient leaching from feces and was easy to perform. Shrimp fed the diet supplemented with probiotic showed higher apparent digestibility of crude protein (84.18±0.35%) and energy (81.28±0.16%) when compared to the control group (82.73±1.01 and 78.63±0.93%, respectively). The use of L. plantarum as a probiotic additive in shrimp diets can provide nutritional benefits to L. vannamei, with better nutrient utilization

Prebiotic, probiotic, and symbiotic‑supplemented diet for marine shrimp farming

O objetivo deste trabalho foi avaliar o uso de suplemento prebiótico, probiótico e simbiótico, na dieta de camarões marinhos (Litopenaeus vannamei) e seus efeitos sobre o crescimento, a microbiota intestinal, a resposta imune e a resistência ao desafio experimental com Vibrio alginolyticus. Foram utilizados quatro tratamentos: prebiótico inulina; probiótico Lactobacillus plantarum; simbiótico Lactobacillus plantarum + inulina; e controle. Os camarões foram distribuídos em 16 tanques de dez mil litros de água, povoados com 200 camarões cada, cultivados por seis semanas. Avaliaram-se a microbiologia do trato intestinal dos camarões e a reposta imune, antes e após o desafio com V. alginolyticus. A concentração de Vibrio spp. no trato digestório foi menor em camarões alimentados com dieta suplementada com prebiótico, probiótico e simbiótico, enquanto a concentração de bactérias acidoláticas foi superior somente nos camarões alimentados com probiótico e simbiótico. O título aglutinante do soro contra V. alginolyticus aumentou no grupo probiótico e simbiótico, antes da infecção, e foi maior em todos os tratamentos após infecção com V. alginolyticus, em comparação ao controle. Não foi observada diferença entre os tratamentos quanto aos demais parâmetros avaliados. As dietas probióticas, prebióticas e simbióticas alteram a microbiota intestinal e aumentam o título aglutinante do soro contra V. alginolyticus; contudo, não alteram a resistência ao desafio nem o crescimento dos camarões.The objective of this work was to evaluate the use of prebiotic, probiotic, and symbiotic supplements, in the diet of shrimp (Litopenaeus vannamei), and their effects on growth, intestinal microbiota, immune response, and resistance to experimental challenge with Vibrio alginolyticus. Four treatments were used: prebiotic (inulin); probiotic (Lactobacillus plantarum); symbiotic (Lactobacillus plantarum + inulin); and a control. Shrimp were distributed in 16 tanks with ten thousand liters of water, stocked with 200 shrimp each, cultivated for six weeks. Microbiology of the intestinal tract and the immune response of shrimp were evaluated before and after the challenge with V. alginolyticus. The concentration of Vibrio spp. in the digestive tract was lower in shrimp fed prebiotic, probiotic, and symbiotic‑supplemented diet, while concentration of lactic acid bacteria was higher only in shrimp fed probiotic and symbiotic‑supplemented diet. The title of agglutinating serum against V. alginolyticus increased in shrimp fed the probiotic and symbiotic‑supplemented diet prior to infection, and was higher in all treatments after infection with V. alginolyticus, in comparison to the control. No difference was observed between treatments for the remaining parameters. Probiotic, prebiotic, and symbiotic diets change intestinal microbiota, and increase the agglutinating serum title against V. alginolyticus; however, they change neither the shrimp resistance to challenge by V. alginolyticus nor shrimp growth